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The map label for this gene is holB [H]

Identifier: 147674380

GI number: 147674380

Start: 1722371

End: 1723345

Strand: Reverse

Name: holB [H]

Synonym: VC0395_A1601

Alternate gene names: 147674380

Gene position: 1723345-1722371 (Counterclockwise)

Preceding gene: 147673617

Following gene: 147674791

Centisome position: 56.99

GC content: 47.9

Gene sequence:

>975_bases
GTGTCTAATCTCAATTCTCTTTACCCTTGGTTAGTTCCCGTTTGGCAGCCTTGGCAAGCGGGATTAGCTGCAGGAAAAAT
CTCGTCAGCGACGTTAATTCAGGCATCAGAAGGAGTAGGCGTTGAGAGTTTGGTTGAACTCATGGCCCGCACCTTGATGT
GTACGAGTAGCCAAAGTGAGCCATGCGGTTTTTGCCATAGTTGCGGTCTAATGCAATCGGGAAATCATCCCGACTTTCAT
GTTGTTAAGCCTGAAAAGATCGGGAAATCGATCACGGTGGAGCAAATTCGCCAGATGAATCGTATCGCCCAAGAGTCGTC
CCAGCTCTCCGGTTATCGCTTGATTGTGATTGAGCCTGCCGATGCGATGAATGAATCTTCCGCCAACGCGCTACTGAAAA
CGCTCGAAGAGCCTGCACCAAACTGTCTATTTATTTTGGTTACCTCTCGGATTAAGCATCTTTTGCCGACGATAGTTAGC
CGTTGCCAAAGATTAGTGCTCCCTGCGCCAACCACTGCGTTGGTGGTTGAGTGGCTAAAAGGGCAGGGGATCACGACGCC
TGCTTACGCCTTACATCTTTGCGCAGATTCACCGCTCAAAACCCGTGCCTTTATGCTAGAAGGGGGCGCGGAAAAATATC
ATGAGCTTGAAAGCCAACTGATGAATGCGCTGAGTGGCGATGTGAATGCGCAACTTAAATGTATCGCACTCATCGATGCC
GATCTGACCACGCATTTGTATTGGGTTTGGTGTGTTTTGACCGATGCGCAAAAAATTCACTTTGGTGTGCAGCAAGACTA
TTATCCCCCAGCAAGTGCCGCATTAGCTGGCCGTTTTACCTATTCTAAACTTCATGTGCAGACAGCGAGCTTGGAAAGGC
TGATGGAACAATTGAACCAGTTCAGTGGATTGAATACAGAACTGCTGTTGCTGCAATGGTTATATCAATTTAGTGATGAG
GAAACATGTTTGTAG

Upstream 100 bases:

>100_bases
AGCGATGATTCTGTGGTTATGATTGATGCAGCACAGTCGATTGAACAGGTCACGGCCGATATTCGTCGTGCTTTACAAGA
TTGGTTGAGTCAAGTTAACC

Downstream 100 bases:

>100_bases
ATTCTCATTGTCATCTCGACAAGTTGGATTACCAAGACTTACACGCGGATGTCAGCGATGTGCTCGACAAAGCGCACCAA
GCCAAAGTGGAATATTTACT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSNLNSLYPWLVPVWQPWQAGLAAGKISSATLIQASEGVGVESLVELMARTLMCTSSQSEPCGFCHSCGLMQSGNHPDFH
VVKPEKIGKSITVEQIRQMNRIAQESSQLSGYRLIVIEPADAMNESSANALLKTLEEPAPNCLFILVTSRIKHLLPTIVS
RCQRLVLPAPTTALVVEWLKGQGITTPAYALHLCADSPLKTRAFMLEGGAEKYHELESQLMNALSGDVNAQLKCIALIDA
DLTTHLYWVWCVLTDAQKIHFGVQQDYYPPASAALAGRFTYSKLHVQTASLERLMEQLNQFSGLNTELLLLQWLYQFSDE
ETCL

Sequences:

>Translated_324_residues
MSNLNSLYPWLVPVWQPWQAGLAAGKISSATLIQASEGVGVESLVELMARTLMCTSSQSEPCGFCHSCGLMQSGNHPDFH
VVKPEKIGKSITVEQIRQMNRIAQESSQLSGYRLIVIEPADAMNESSANALLKTLEEPAPNCLFILVTSRIKHLLPTIVS
RCQRLVLPAPTTALVVEWLKGQGITTPAYALHLCADSPLKTRAFMLEGGAEKYHELESQLMNALSGDVNAQLKCIALIDA
DLTTHLYWVWCVLTDAQKIHFGVQQDYYPPASAALAGRFTYSKLHVQTASLERLMEQLNQFSGLNTELLLLQWLYQFSDE
ETCL
>Mature_323_residues
SNLNSLYPWLVPVWQPWQAGLAAGKISSATLIQASEGVGVESLVELMARTLMCTSSQSEPCGFCHSCGLMQSGNHPDFHV
VKPEKIGKSITVEQIRQMNRIAQESSQLSGYRLIVIEPADAMNESSANALLKTLEEPAPNCLFILVTSRIKHLLPTIVSR
CQRLVLPAPTTALVVEWLKGQGITTPAYALHLCADSPLKTRAFMLEGGAEKYHELESQLMNALSGDVNAQLKCIALIDAD
LTTHLYWVWCVLTDAQKIHFGVQQDYYPPASAALAGRFTYSKLHVQTASLERLMEQLNQFSGLNTELLLLQWLYQFSDEE
TCL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=321, Percent_Identity=31.1526479750779, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786676, Length=163, Percent_Identity=32.5153374233129, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 35851; Mature: 35720

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLNSLYPWLVPVWQPWQAGLAAGKISSATLIQASEGVGVESLVELMARTLMCTSSQSE
CCCCCCCCHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCC
PCGFCHSCGLMQSGNHPDFHVVKPEKIGKSITVEQIRQMNRIAQESSQLSGYRLIVIEPA
CCCHHHHCCCCCCCCCCCEEEECHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCC
DAMNESSANALLKTLEEPAPNCLFILVTSRIKHLLPTIVSRCQRLVLPAPTTALVVEWLK
HHCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
GQGITTPAYALHLCADSPLKTRAFMLEGGAEKYHELESQLMNALSGDVNAQLKCIALIDA
CCCCCCHHHHHEECCCCCCHHHHEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
DLTTHLYWVWCVLTDAQKIHFGVQQDYYPPASAALAGRFTYSKLHVQTASLERLMEQLNQ
CHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHHHCEEHHHHHHHHHHHHHHHHHHHH
FSGLNTELLLLQWLYQFSDEETCL
HCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SNLNSLYPWLVPVWQPWQAGLAAGKISSATLIQASEGVGVESLVELMARTLMCTSSQSE
CCCCCCCHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCC
PCGFCHSCGLMQSGNHPDFHVVKPEKIGKSITVEQIRQMNRIAQESSQLSGYRLIVIEPA
CCCHHHHCCCCCCCCCCCEEEECHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCC
DAMNESSANALLKTLEEPAPNCLFILVTSRIKHLLPTIVSRCQRLVLPAPTTALVVEWLK
HHCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
GQGITTPAYALHLCADSPLKTRAFMLEGGAEKYHELESQLMNALSGDVNAQLKCIALIDA
CCCCCCHHHHHEECCCCCCHHHHEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
DLTTHLYWVWCVLTDAQKIHFGVQQDYYPPASAALAGRFTYSKLHVQTASLERLMEQLNQ
CHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHHHCEEHHHHHHHHHHHHHHHHHHHH
FSGLNTELLLLQWLYQFSDEETCL
HCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]