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The map label for this gene is era [H]

Identifier: 147674346

GI number: 147674346

Start: 2195711

End: 2196685

Strand: Reverse

Name: era [H]

Synonym: VC0395_A2038

Alternate gene names: 147674346

Gene position: 2196685-2195711 (Counterclockwise)

Preceding gene: 147674970

Following gene: 147673381

Centisome position: 72.64

GC content: 49.03

Gene sequence:

>975_bases
ATGGCTGATCAAGAATTTGATATCGATGCATACTTTGCTTCTCGCAGCGAAGGTCAAACGGCGACATCGACACCAGAGGA
CCAACACTGCGGCTTTGTCGCAATTGTCGGTCGTCCTAACGTGGGGAAATCGACCCTGCTGAATAACCTTTTAGGGCAGA
AGATTTCGATCACCTCGCGTAAACCACAAACTACTCGCCACCGTATTATGGGCGTGGAGACCGATGGCAATTATCAGGCG
ATATACGTTGATACCCCAGGACTACACATTGAAGAAAAACGCGCCATTAACCGTTTGATGAACCGTGCGGCGTCAAGCTC
ATTGAGTGATGTCAACTTAGTGCTGTTTGTGGTGGAAGGTACTCACTGGACCGCCGATGACGAGATGGTGTTCAACAAGC
TGCAAAAAGCCAACTTCCCGGTGGTGCTTTGTGTGAACAAGGTGGATCAGGTGAAAGATCGTAATGAAGTGATGCTGCAC
ATGCTGGAGCTTTCTAAGCGCATGCAGTTTGTCGATATCGTACCGATTTCCGCTAAACAGGGTAAAAACACCGACGTGCT
GAAAAAGCATGTACGCGACCATTTACCGAAAGCTGTGCACCATTTCCCTGAAGAGTATGTGACGGATCGTTCACAGCGCT
TTATGGCATCTGAAATCGTGCGTGAAAAGCTGATGCGTTTTACTGGCGAAGAGCTGCCGTATTCGGTGACGGTGGAAATC
GAGCGTTTCGATTACAACCCAGATACCGATGGTTTCCACATCAACGCCCTGATTTTGGTTGAGCGTATCGGCCAGAAGAA
AATGGTGATTGGCAAAAACGGCGAGAAGATTAAAACCATCGGTCGTGAAGCACGTCTCGATATGGAAGAGCTGTTTGGCC
GCAAAGTCTACCTTGAGACTTGGGTTAAAGTGAAATCTGGGTGGGCGGATGATGAGCGCGCCCTGCGTTCACTCGGCTAT
ATCGACGATCTCTAA

Upstream 100 bases:

>100_bases
TGTGAAGTTGCAGGTATGGACACGCCTGTGATCGGTAAAGGCACCAGCCGCCGCAAGGCAGAGCAAGCGGCTGCAGAAAC
GGCTCTGGAGCAATTAACTC

Downstream 100 bases:

>100_bases
ACACGAGAGGTGGCAACGGTGTTGCCACCTTTTTCATTTCTACCTTCAACTGCATGATAGAAAGGGAAAATGTCAGACGG
ACTGCAACGCTGTTTTGTTC

Product: GTP-binding protein Era

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MADQEFDIDAYFASRSEGQTATSTPEDQHCGFVAIVGRPNVGKSTLLNNLLGQKISITSRKPQTTRHRIMGVETDGNYQA
IYVDTPGLHIEEKRAINRLMNRAASSSLSDVNLVLFVVEGTHWTADDEMVFNKLQKANFPVVLCVNKVDQVKDRNEVMLH
MLELSKRMQFVDIVPISAKQGKNTDVLKKHVRDHLPKAVHHFPEEYVTDRSQRFMASEIVREKLMRFTGEELPYSVTVEI
ERFDYNPDTDGFHINALILVERIGQKKMVIGKNGEKIKTIGREARLDMEELFGRKVYLETWVKVKSGWADDERALRSLGY
IDDL

Sequences:

>Translated_324_residues
MADQEFDIDAYFASRSEGQTATSTPEDQHCGFVAIVGRPNVGKSTLLNNLLGQKISITSRKPQTTRHRIMGVETDGNYQA
IYVDTPGLHIEEKRAINRLMNRAASSSLSDVNLVLFVVEGTHWTADDEMVFNKLQKANFPVVLCVNKVDQVKDRNEVMLH
MLELSKRMQFVDIVPISAKQGKNTDVLKKHVRDHLPKAVHHFPEEYVTDRSQRFMASEIVREKLMRFTGEELPYSVTVEI
ERFDYNPDTDGFHINALILVERIGQKKMVIGKNGEKIKTIGREARLDMEELFGRKVYLETWVKVKSGWADDERALRSLGY
IDDL
>Mature_323_residues
ADQEFDIDAYFASRSEGQTATSTPEDQHCGFVAIVGRPNVGKSTLLNNLLGQKISITSRKPQTTRHRIMGVETDGNYQAI
YVDTPGLHIEEKRAINRLMNRAASSSLSDVNLVLFVVEGTHWTADDEMVFNKLQKANFPVVLCVNKVDQVKDRNEVMLHM
LELSKRMQFVDIVPISAKQGKNTDVLKKHVRDHLPKAVHHFPEEYVTDRSQRFMASEIVREKLMRFTGEELPYSVTVEIE
RFDYNPDTDGFHINALILVERIGQKKMVIGKNGEKIKTIGREARLDMEELFGRKVYLETWVKVKSGWADDERALRSLGYI
DDL

Specific function: An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism [H]

COG id: COG1159

COG function: function code R; GTPase

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KH type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI24307899, Length=321, Percent_Identity=24.9221183800623, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788919, Length=296, Percent_Identity=72.2972972972973, Blast_Score=446, Evalue=1e-127,
Organism=Escherichia coli, GI87082120, Length=182, Percent_Identity=30.7692307692308, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646434, Length=308, Percent_Identity=23.7012987012987, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005662
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004044
- InterPro:   IPR002917
- InterPro:   IPR005225 [H]

Pfam domain/function: PF07650 KH_2; PF01926 MMR_HSR1 [H]

EC number: NA

Molecular weight: Translated: 37026; Mature: 36895

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50823 KH_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQEFDIDAYFASRSEGQTATSTPEDQHCGFVAIVGRPNVGKSTLLNNLLGQKISITSR
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEECCC
KPQTTRHRIMGVETDGNYQAIYVDTPGLHIEEKRAINRLMNRAASSSLSDVNLVLFVVEG
CCCCCHHEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEC
THWTADDEMVFNKLQKANFPVVLCVNKVDQVKDRNEVMLHMLELSKRMQFVDIVPISAKQ
CEECCCHHHHHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHHHHCCEEEEEEECCCCC
GKNTDVLKKHVRDHLPKAVHHFPEEYVTDRSQRFMASEIVREKLMRFTGEELPYSVTVEI
CCCHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
ERFDYNPDTDGFHINALILVERIGQKKMVIGKNGEKIKTIGREARLDMEELFGRKVYLET
EEECCCCCCCCEEEEHEEEEHHCCCCEEEECCCCCHHHHHHHHHCCCHHHHCCCEEEEHH
WVKVKSGWADDERALRSLGYIDDL
HHHHHCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADQEFDIDAYFASRSEGQTATSTPEDQHCGFVAIVGRPNVGKSTLLNNLLGQKISITSR
CCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEECCC
KPQTTRHRIMGVETDGNYQAIYVDTPGLHIEEKRAINRLMNRAASSSLSDVNLVLFVVEG
CCCCCHHEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEC
THWTADDEMVFNKLQKANFPVVLCVNKVDQVKDRNEVMLHMLELSKRMQFVDIVPISAKQ
CEECCCHHHHHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHHHHCCEEEEEEECCCCC
GKNTDVLKKHVRDHLPKAVHHFPEEYVTDRSQRFMASEIVREKLMRFTGEELPYSVTVEI
CCCHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
ERFDYNPDTDGFHINALILVERIGQKKMVIGKNGEKIKTIGREARLDMEELFGRKVYLET
EEECCCCCCCCEEEEHEEEEHHCCCCEEEECCCCCHHHHHHHHHCCCHHHHCCCEEEEHH
WVKVKSGWADDERALRSLGYIDDL
HHHHHCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]