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The map label for this gene is cysT [H]

Identifier: 147674327

GI number: 147674327

Start: 61822

End: 62673

Strand: Direct

Name: cysT [H]

Synonym: VC0395_A0066

Alternate gene names: 147674327

Gene position: 61822-62673 (Clockwise)

Preceding gene: 147673555

Following gene: 147673965

Centisome position: 2.04

GC content: 51.29

Gene sequence:

>852_bases
ATGGGTCAAACGCTTGCACACTCTCGCCCGCGCCAGCAACGGGTTTTACCCGGTTTTGCGTTGAGCCTCGGTGTCTCTTT
GCTGTTTGTCAGCTTGATCCTGCTGCTGCCTGCCACTGGGCTTATCATGCAGACCAGCAACATGACCTTTAGCGAGTATT
GGCAAGTGATTGCCGACCCTCGTGTGGTGGCAAGTTATAAAGTAACGGTTGGCTCGGCCTTGATTGCTTCACTGTTTAAT
GGCTTGTTTGGTTTGCTGCTTGCTTGGGTGTTGGTGCGTTACACTTTTCCCGGCAAACGCATTCTTGATGCGCTGGTCGA
TCTTCCTTTTGCGCTGCCCACCGCGGTGGCAGGGATTACCTTGGCAACGCTGTATTCGGCCAATGGCTGGATTGGTAGCG
TACTTGCTGAGTTAGGCATCAAGGTCGCTTATACGCCACTTGGTATTATTGTGGCGATGGCTTTTACCAGTATTCCCTTT
GTGGTGCGCACGGTTCAGCCTGTGTTAGAAGAAATTTCCCGTGAAGAAGAAGAAGCGGGCATGACGTTAGGTGCATCCGA
CGCTGCTGTGTTTTGGAAAGTGATTCTCCCTTCACTCAAACCCGCTTTATTGGTCGGAACCGCGTTGTCTTTTACGCGCA
GTTTAGGGGAGTTCGGAGCGGTGATCTTTATTGCCGGCAACATGCCTTACATCAGCGAAATTACCTCGTTAATGATCTTC
GTGCGTCTGCAAGAGTTTGATTTCCCAGCGGCGAGTGCGATTGCCTCGGTTGTGCTGCTGACTTCACTTTTGTTACTGCT
GATGATTAACCTCTGGCAAGGCGCTTATCTGCGTCGCATTCACGGACGATAA

Upstream 100 bases:

>100_bases
TTGGATCAACTGCTGCGCCGCTAATCTTGTCTGACATTTCTCTAGGTTGCCCAATGGATGGGCAACCTTAATTCCACCCT
GATGTATCGAAAGAGGTTTT

Downstream 100 bases:

>100_bases
GGAATCAGTGTATGGCACATTCTCAGCCTTTACGTGTTGGTGAAAAGCCGTGGGTGAAATGGAGTTTGATCGCATTGGCT
CTGCTATTGGTGGCCGTACT

Product: sulfate ABC transporter, permease protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MGQTLAHSRPRQQRVLPGFALSLGVSLLFVSLILLLPATGLIMQTSNMTFSEYWQVIADPRVVASYKVTVGSALIASLFN
GLFGLLLAWVLVRYTFPGKRILDALVDLPFALPTAVAGITLATLYSANGWIGSVLAELGIKVAYTPLGIIVAMAFTSIPF
VVRTVQPVLEEISREEEEAGMTLGASDAAVFWKVILPSLKPALLVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIF
VRLQEFDFPAASAIASVVLLTSLLLLLMINLWQGAYLRRIHGR

Sequences:

>Translated_283_residues
MGQTLAHSRPRQQRVLPGFALSLGVSLLFVSLILLLPATGLIMQTSNMTFSEYWQVIADPRVVASYKVTVGSALIASLFN
GLFGLLLAWVLVRYTFPGKRILDALVDLPFALPTAVAGITLATLYSANGWIGSVLAELGIKVAYTPLGIIVAMAFTSIPF
VVRTVQPVLEEISREEEEAGMTLGASDAAVFWKVILPSLKPALLVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIF
VRLQEFDFPAASAIASVVLLTSLLLLLMINLWQGAYLRRIHGR
>Mature_282_residues
GQTLAHSRPRQQRVLPGFALSLGVSLLFVSLILLLPATGLIMQTSNMTFSEYWQVIADPRVVASYKVTVGSALIASLFNG
LFGLLLAWVLVRYTFPGKRILDALVDLPFALPTAVAGITLATLYSANGWIGSVLAELGIKVAYTPLGIIVAMAFTSIPFV
VRTVQPVLEEISREEEEAGMTLGASDAAVFWKVILPSLKPALLVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIFV
RLQEFDFPAASAIASVVLLTSLLLLLMINLWQGAYLRRIHGR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=271, Percent_Identity=67.8966789667897, Blast_Score=327, Evalue=7e-91,
Organism=Escherichia coli, GI87082099, Length=239, Percent_Identity=35.5648535564854, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1786980, Length=232, Percent_Identity=34.9137931034483, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787369, Length=241, Percent_Identity=24.4813278008299, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787080, Length=223, Percent_Identity=27.3542600896861, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786254, Length=228, Percent_Identity=25, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30588; Mature: 30457

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQTLAHSRPRQQRVLPGFALSLGVSLLFVSLILLLPATGLIMQTSNMTFSEYWQVIADP
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCC
RVVASYKVTVGSALIASLFNGLFGLLLAWVLVRYTFPGKRILDALVDLPFALPTAVAGIT
HHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHH
LATLYSANGWIGSVLAELGIKVAYTPLGIIVAMAFTSIPFVVRTVQPVLEEISREEEEAG
HHHHHHCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MTLGASDAAVFWKVILPSLKPALLVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIF
CEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHH
VRLQEFDFPAASAIASVVLLTSLLLLLMINLWQGAYLRRIHGR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GQTLAHSRPRQQRVLPGFALSLGVSLLFVSLILLLPATGLIMQTSNMTFSEYWQVIADP
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCC
RVVASYKVTVGSALIASLFNGLFGLLLAWVLVRYTFPGKRILDALVDLPFALPTAVAGIT
HHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHH
LATLYSANGWIGSVLAELGIKVAYTPLGIIVAMAFTSIPFVVRTVQPVLEEISREEEEAG
HHHHHHCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MTLGASDAAVFWKVILPSLKPALLVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIF
CEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHH
VRLQEFDFPAASAIASVVLLTSLLLLLMINLWQGAYLRRIHGR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]