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The map label for this gene is cysP [H]

Identifier: 147673555

GI number: 147673555

Start: 60744

End: 61745

Strand: Direct

Name: cysP [H]

Synonym: VC0395_A0065

Alternate gene names: 147673555

Gene position: 60744-61745 (Clockwise)

Preceding gene: 147674644

Following gene: 147674327

Centisome position: 2.01

GC content: 48.9

Gene sequence:

>1002_bases
ATGAACCGAATTAAATCGACATTAACAGCGTTACTGCTAGCAGGCTCGTTCCAAGCCTCTGCGGCCGATCAAACCATTCT
CAACTCATCTTATGATATTGCTCGTGAGCTGTTTGTGGCTTACAACCCTGTGTTTGCTGAGCATTGGCAACAAAAAACAG
GGAAGACGGTCGAAATTAAACAGTCGCACGCTGGATCATCGGCTCAAGCGCGTTCTATTCTGCAAGGCCTAGCGGCAGAT
GTGGTGACGTTTAACCAAGTGACCGATGTGCAAATTCTGCACGACAAAGGCAAGCTGATCTCAGCAAACTGGCAAACCCT
GCTACCAAACGCAAGCTCGCCTTATTACTCCACCACCGCATTTTTGGTGCGCAAAGGCAACCCAAAGAACATCCAAGATT
GGAGTGATTTGGTACGTGATGATGTGAAAAGTGTGTTCCCGAACCCGAAAACCTCAGGTAATGCGCGTTACACCTACTTG
GCAGCGCTAGGCTTTGCACAAAAACAATTTGGTCAGGATAACCAAGTGCAGCAAGATGAGTTTTTGAAGAAGTTTCTGGC
TAACGTCGCGGTATTTGATACTGGCGGTCGTGGTGCAACCACATCATTCGTTGAGCGTGGGATTGGCGATGTGCTGATCA
CCTTTGAATCGGAAGTGAATAACATTCGTCAGCAATACGGTGCGGATGATTACCAAGTGGTGGTGCCGAAAACCTCGATT
TTGGCTGAATTCCCTGTCGCGGTAGTGGAGAAAGTGGCAAAACGTAATGGGACTTATGATGTGGCGACCGAATACGTTTC
ATACCTGTACAGTGAGCCAGCACAGCGTTTGCTTGCAGAATTTAACTACCGTGTGCACGATGCCAAAGTACAAGCTGAGT
TTGCTGAGCGTTTTCCCGCCGTTGAATTGCTGACTGTGGAAGGGATCGCCGGTGGCTGGGAGCAAGCGATGAAAACCCAG
TTCGCCAATGGGGCTAAATTGGATCAACTGCTGCGCCGCTAA

Upstream 100 bases:

>100_bases
AAAACGTTCTATTAATGTTCTTTTTTATAAACGATTTTCCTCATAATCTAGCAGCATAAAGGAAGCCATCTTGGCACTTG
TGATTCTGGAGTAGAACAAC

Downstream 100 bases:

>100_bases
TCTTGTCTGACATTTCTCTAGGTTGCCCAATGGATGGGCAACCTTAATTCCACCCTGATGTATCGAAAGAGGTTTTATGG
GTCAAACGCTTGCACACTCT

Product: thiosulfate ABC transporter, periplasmic thiosulfate-binding protein

Products: SO42- [Cytoplasm]; phosphate; ADP; S2O32- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNRIKSTLTALLLAGSFQASAADQTILNSSYDIARELFVAYNPVFAEHWQQKTGKTVEIKQSHAGSSAQARSILQGLAAD
VVTFNQVTDVQILHDKGKLISANWQTLLPNASSPYYSTTAFLVRKGNPKNIQDWSDLVRDDVKSVFPNPKTSGNARYTYL
AALGFAQKQFGQDNQVQQDEFLKKFLANVAVFDTGGRGATTSFVERGIGDVLITFESEVNNIRQQYGADDYQVVVPKTSI
LAEFPVAVVEKVAKRNGTYDVATEYVSYLYSEPAQRLLAEFNYRVHDAKVQAEFAERFPAVELLTVEGIAGGWEQAMKTQ
FANGAKLDQLLRR

Sequences:

>Translated_333_residues
MNRIKSTLTALLLAGSFQASAADQTILNSSYDIARELFVAYNPVFAEHWQQKTGKTVEIKQSHAGSSAQARSILQGLAAD
VVTFNQVTDVQILHDKGKLISANWQTLLPNASSPYYSTTAFLVRKGNPKNIQDWSDLVRDDVKSVFPNPKTSGNARYTYL
AALGFAQKQFGQDNQVQQDEFLKKFLANVAVFDTGGRGATTSFVERGIGDVLITFESEVNNIRQQYGADDYQVVVPKTSI
LAEFPVAVVEKVAKRNGTYDVATEYVSYLYSEPAQRLLAEFNYRVHDAKVQAEFAERFPAVELLTVEGIAGGWEQAMKTQ
FANGAKLDQLLRR
>Mature_333_residues
MNRIKSTLTALLLAGSFQASAADQTILNSSYDIARELFVAYNPVFAEHWQQKTGKTVEIKQSHAGSSAQARSILQGLAAD
VVTFNQVTDVQILHDKGKLISANWQTLLPNASSPYYSTTAFLVRKGNPKNIQDWSDLVRDDVKSVFPNPKTSGNARYTYL
AALGFAQKQFGQDNQVQQDEFLKKFLANVAVFDTGGRGATTSFVERGIGDVLITFESEVNNIRQQYGADDYQVVVPKTSI
LAEFPVAVVEKVAKRNGTYDVATEYVSYLYSEPAQRLLAEFNYRVHDAKVQAEFAERFPAVELLTVEGIAGGWEQAMKTQ
FANGAKLDQLLRR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. This protein specifically binds thiosulfate and is involved in its transmembrane transport [H]

COG id: COG4150

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1788765, Length=307, Percent_Identity=61.8892508143323, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1790351, Length=333, Percent_Identity=46.2462462462462, Blast_Score=298, Evalue=5e-82,

Paralogues:

None

Copy number: 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36911; Mature: 36911

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIKSTLTALLLAGSFQASAADQTILNSSYDIARELFVAYNPVFAEHWQQKTGKTVEIK
CCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEE
QSHAGSSAQARSILQGLAADVVTFNQVTDVQILHDKGKLISANWQTLLPNASSPYYSTTA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCEECCCCCCCCEEEEE
FLVRKGNPKNIQDWSDLVRDDVKSVFPNPKTSGNARYTYLAALGFAQKQFGQDNQVQQDE
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCHHH
FLKKFLANVAVFDTGGRGATTSFVERGIGDVLITFESEVNNIRQQYGADDYQVVVPKTSI
HHHHHHHHEEEEECCCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCHHH
LAEFPVAVVEKVAKRNGTYDVATEYVSYLYSEPAQRLLAEFNYRVHDAKVQAEFAERFPA
HHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
VELLTVEGIAGGWEQAMKTQFANGAKLDQLLRR
EEEEEECCCCCHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure
MNRIKSTLTALLLAGSFQASAADQTILNSSYDIARELFVAYNPVFAEHWQQKTGKTVEIK
CCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEE
QSHAGSSAQARSILQGLAADVVTFNQVTDVQILHDKGKLISANWQTLLPNASSPYYSTTA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCEECCCCCCCCEEEEE
FLVRKGNPKNIQDWSDLVRDDVKSVFPNPKTSGNARYTYLAALGFAQKQFGQDNQVQQDE
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCHHH
FLKKFLANVAVFDTGGRGATTSFVERGIGDVLITFESEVNNIRQQYGADDYQVVVPKTSI
HHHHHHHHEEEEECCCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCHHH
LAEFPVAVVEKVAKRNGTYDVATEYVSYLYSEPAQRLLAEFNYRVHDAKVQAEFAERFPA
HHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
VELLTVEGIAGGWEQAMKTQFANGAKLDQLLRR
EEEEEECCCCCHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: SO42- [Periplasm]; H2O; ATP; S2O32- [Periplasm] [C]

Specific reaction: SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188959; 9205837; 9278503; 9298646 [H]