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The map label for this gene is cysW [H]

Identifier: 147673965

GI number: 147673965

Start: 62685

End: 63548

Strand: Direct

Name: cysW [H]

Synonym: VC0395_A0067

Alternate gene names: 147673965

Gene position: 62685-63548 (Clockwise)

Preceding gene: 147674327

Following gene: 147674663

Centisome position: 2.07

GC content: 51.5

Gene sequence:

>864_bases
ATGGCACATTCTCAGCCTTTACGTGTTGGTGAAAAGCCGTGGGTGAAATGGAGTTTGATCGCATTGGCTCTGCTATTGGT
GGCCGTACTGCTGCTGATCCCACTGCTGAGCATTTTCCAACAAGCGTTTGCCAACGGCATTGGCGCTTATTTTAGTTACT
TTAATGACCCCGATACGCGCCACGCGATTGGGTTAACGCTGGTGGTGGCGCTACTGACAGTGCCAATCAATCTGGTGTTT
GGTGTGCTGCTCTCTTGGCTAGTGACCCGCTTTGAATTTGTTGGTCGCAAGTTTTTGATCACCTTGATTGATATTCCGTT
CGCTGTTTCTCCGGTGGTGGCGGGGTTACTTTACCTGCTGCTCTATGGCACCAGTGGCTGGTTGGGTGAATGGTTGTATG
AGCGCGATCTGCAAATCATGTTTGCTTGGCCGGGCATTGTACTGGTGACTGTGTTTGTGACCTGTCCGTTTGTGGCGCGT
GAGCTAATTCCACTTATGCAGCAGCAAGGACGTTCGGATGAAGAAGCCGCAGTGATTTTGGGCGCATCATGGTGGCAACT
GTTTCGCCGAGTCACTCTGCCTAACATTAAGTGGGCGCTGATTTATGGCGTGATCCTCACCAATGCTCGCGCGGTGGGGG
AGTTTGGCGCGGTAGCGGTGGTTTCAGGCAACATTCGTGGTGAAACCAATACCTTGCCTTTGCATGTACAGCTTCTGTAC
GAGGATTATCAATCGCAAGCGGCCTTTGCTAGCGCTTCGCTACTGGCGTTTATCGCCCTATTAACACTATTACTGAAAGC
TTTGGTCGAGTGGCGTCAAGAGCGCCTTCAGGCGGTCGAGCATAATGAGCAGGGAACCTTATGA

Upstream 100 bases:

>100_bases
CCTCGGTTGTGCTGCTGACTTCACTTTTGTTACTGCTGATGATTAACCTCTGGCAAGGCGCTTATCTGCGTCGCATTCAC
GGACGATAAGGAATCAGTGT

Downstream 100 bases:

>100_bases
GCATTCGTCTAGATAATATCTCTAAACATTTTGGCCAGTTTCAGGCGCTTGCCCCGCTTTCTCTCAAGATTGAAGATGGT
GAAATGATTGGTTTGTTAGG

Product: sulfate ABC transporter, permease protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MAHSQPLRVGEKPWVKWSLIALALLLVAVLLLIPLLSIFQQAFANGIGAYFSYFNDPDTRHAIGLTLVVALLTVPINLVF
GVLLSWLVTRFEFVGRKFLITLIDIPFAVSPVVAGLLYLLLYGTSGWLGEWLYERDLQIMFAWPGIVLVTVFVTCPFVAR
ELIPLMQQQGRSDEEAAVILGASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLY
EDYQSQAAFASASLLAFIALLTLLLKALVEWRQERLQAVEHNEQGTL

Sequences:

>Translated_287_residues
MAHSQPLRVGEKPWVKWSLIALALLLVAVLLLIPLLSIFQQAFANGIGAYFSYFNDPDTRHAIGLTLVVALLTVPINLVF
GVLLSWLVTRFEFVGRKFLITLIDIPFAVSPVVAGLLYLLLYGTSGWLGEWLYERDLQIMFAWPGIVLVTVFVTCPFVAR
ELIPLMQQQGRSDEEAAVILGASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLY
EDYQSQAAFASASLLAFIALLTLLLKALVEWRQERLQAVEHNEQGTL
>Mature_286_residues
AHSQPLRVGEKPWVKWSLIALALLLVAVLLLIPLLSIFQQAFANGIGAYFSYFNDPDTRHAIGLTLVVALLTVPINLVFG
VLLSWLVTRFEFVGRKFLITLIDIPFAVSPVVAGLLYLLLYGTSGWLGEWLYERDLQIMFAWPGIVLVTVFVTCPFVARE
LIPLMQQQGRSDEEAAVILGASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLYE
DYQSQAAFASASLLAFIALLTLLLKALVEWRQERLQAVEHNEQGTL

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=287, Percent_Identity=58.5365853658537, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1788764, Length=204, Percent_Identity=39.7058823529412, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1786980, Length=219, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32003; Mature: 31872

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHSQPLRVGEKPWVKWSLIALALLLVAVLLLIPLLSIFQQAFANGIGAYFSYFNDPDTR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
HAIGLTLVVALLTVPINLVFGVLLSWLVTRFEFVGRKFLITLIDIPFAVSPVVAGLLYLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LYGTSGWLGEWLYERDLQIMFAWPGIVLVTVFVTCPFVARELIPLMQQQGRSDEEAAVIL
HHCCCCHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
GASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLY
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCCCCEEEHHHHH
EDYQSQAAFASASLLAFIALLTLLLKALVEWRQERLQAVEHNEQGTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AHSQPLRVGEKPWVKWSLIALALLLVAVLLLIPLLSIFQQAFANGIGAYFSYFNDPDTR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
HAIGLTLVVALLTVPINLVFGVLLSWLVTRFEFVGRKFLITLIDIPFAVSPVVAGLLYLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LYGTSGWLGEWLYERDLQIMFAWPGIVLVTVFVTCPFVARELIPLMQQQGRSDEEAAVIL
HHCCCCHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
GASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLY
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCCCCEEEHHHHH
EDYQSQAAFASASLLAFIALLTLLLKALVEWRQERLQAVEHNEQGTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]