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The map label for this gene is murA [H]

Identifier: 147674274

GI number: 147674274

Start: 2255557

End: 2256816

Strand: Reverse

Name: murA [H]

Synonym: VC0395_A2096

Alternate gene names: 147674274

Gene position: 2256816-2255557 (Counterclockwise)

Preceding gene: 147673378

Following gene: 147674609

Centisome position: 74.63

GC content: 51.59

Gene sequence:

>1260_bases
ATGGAAAAGTTTCGAGTTATTGGGTCCACGCAGCCACTCCAAGGCGAAGTGACCATTTCAGGCGCAAAGAATGCCGCCCT
ACCGATTTTATTTGCTTCAATTTTGGCGGAAGAGCCAGTTGAAGTGGCCAATGTGCCTCACCTACGTGACATCGATACCA
CTATGGAACTGCTTGAGCGTTTAGGCGCGAAAGTTGAGCGTAATGGCTCTGTGCATGTGGACGCAGGTCCAATCAACCAA
TATTGCGCACCTTATGATTTGGTAAAAACCATGCGTGCCTCGATTTGGGCCTTAGGTCCATTGGTGGCGCGTTTTGGTCA
AGGTCAAGTTTCTCTGCCGGGGGGCTGTGCAATTGGTGCCCGCCCTGTGGATCTGCATATCCATGGTTTGGAGCAGCTTG
GCGCAACCATTACCTTGGAAGATGGCTATGTAAAAGCCCACGTTGATGGTCGTTTGCAAGGCGCGCATATTGTGATGGAT
AAAGTGAGCGTGGGTGCCACGATCACCATCATGTGTGCCGCAACACTCGCAGAAGGCACTACCGTACTGGATAACGCGGC
GCGTGAACCTGAGATTGTGGATACAGCTATGTTCCTCAATAAACTGGGTGCCAAAATCTCTGGTGCCGGCACGGACAGCA
TTACCATTGAGGGCGTAGAACGTCTTGGCGGTGGTAAGCATGCTGTGGTTCCCGATCGTATCGAAACCGGCACCTTCTTG
GTCGCCGCTGCCGTATCGCGTGGCAAAATTGTTTGCCGTAACACTCATGCACATCTATTGGAAGCGGTCTTAGCTAAGCT
TGAAGAAGCGGGTGCTGAGATTGAGTGTGGCGAAGATTGGATCAGTTTGGATATGACAGGGCGTGAGCTCAAAGCGGTTA
CGGTACGTACTGCGCCGCACCCAGGTTTTCCAACCGACATGCAAGCGCAGTTCACACTGCTGAACATGATGGCGAAAGGC
GGTGGCGTGATCACCGAAACCATTTTCGAAAACCGCTTTATGCATGTACCTGAGTTAAAACGCATGGGTGCCAAAGCGGA
AATCGAAGGCAATACCGTGATTTGCGGTGATGTCGATCGTTTAAGTGGCGCGCAAGTGATGGCGACCGATCTGCGTGCTT
CTGCAAGTCTGGTCATTGCAGGTTGTATCGCCAAAGGCGAAACCATTGTCGATCGTATCTATCACATTGATCGTGGTTAC
GAGCGAATTGAGGACAAACTCTCAGCGCTTGGTGCCAATATTGAGCGTTTCCGCGACTAG

Upstream 100 bases:

>100_bases
GGTTACATCCAACGTAATGATATTCATGCGGTCACAATGAAGACGTTTACCCCACAAGAGTGGGCACGCAATAAGAAGTT
GATGTCCCTTTAAGGTTTTT

Downstream 100 bases:

>100_bases
CGTTAAACTCCTCATTCATGAATGGGCCGAAATCCAAGGGTTTCGGCCTTTTTCTTCCAGTGCCTTCGGAGAAGTTGCAT
GATTGCGTTATTGCGAATCT

Product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Products: NA

Alternate protein names: Enoylpyruvate transferase; UDP-N-acetylglucosamine enolpyruvyl transferase; EPT [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MEKFRVIGSTQPLQGEVTISGAKNAALPILFASILAEEPVEVANVPHLRDIDTTMELLERLGAKVERNGSVHVDAGPINQ
YCAPYDLVKTMRASIWALGPLVARFGQGQVSLPGGCAIGARPVDLHIHGLEQLGATITLEDGYVKAHVDGRLQGAHIVMD
KVSVGATITIMCAATLAEGTTVLDNAAREPEIVDTAMFLNKLGAKISGAGTDSITIEGVERLGGGKHAVVPDRIETGTFL
VAAAVSRGKIVCRNTHAHLLEAVLAKLEEAGAEIECGEDWISLDMTGRELKAVTVRTAPHPGFPTDMQAQFTLLNMMAKG
GGVITETIFENRFMHVPELKRMGAKAEIEGNTVICGDVDRLSGAQVMATDLRASASLVIAGCIAKGETIVDRIYHIDRGY
ERIEDKLSALGANIERFRD

Sequences:

>Translated_419_residues
MEKFRVIGSTQPLQGEVTISGAKNAALPILFASILAEEPVEVANVPHLRDIDTTMELLERLGAKVERNGSVHVDAGPINQ
YCAPYDLVKTMRASIWALGPLVARFGQGQVSLPGGCAIGARPVDLHIHGLEQLGATITLEDGYVKAHVDGRLQGAHIVMD
KVSVGATITIMCAATLAEGTTVLDNAAREPEIVDTAMFLNKLGAKISGAGTDSITIEGVERLGGGKHAVVPDRIETGTFL
VAAAVSRGKIVCRNTHAHLLEAVLAKLEEAGAEIECGEDWISLDMTGRELKAVTVRTAPHPGFPTDMQAQFTLLNMMAKG
GGVITETIFENRFMHVPELKRMGAKAEIEGNTVICGDVDRLSGAQVMATDLRASASLVIAGCIAKGETIVDRIYHIDRGY
ERIEDKLSALGANIERFRD
>Mature_419_residues
MEKFRVIGSTQPLQGEVTISGAKNAALPILFASILAEEPVEVANVPHLRDIDTTMELLERLGAKVERNGSVHVDAGPINQ
YCAPYDLVKTMRASIWALGPLVARFGQGQVSLPGGCAIGARPVDLHIHGLEQLGATITLEDGYVKAHVDGRLQGAHIVMD
KVSVGATITIMCAATLAEGTTVLDNAAREPEIVDTAMFLNKLGAKISGAGTDSITIEGVERLGGGKHAVVPDRIETGTFL
VAAAVSRGKIVCRNTHAHLLEAVLAKLEEAGAEIECGEDWISLDMTGRELKAVTVRTAPHPGFPTDMQAQFTLLNMMAKG
GGVITETIFENRFMHVPELKRMGAKAEIEGNTVICGDVDRLSGAQVMATDLRASASLVIAGCIAKGETIVDRIYHIDRGY
ERIEDKLSALGANIERFRD

Specific function: Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine [H]

COG id: COG0766

COG function: function code M; UDP-N-acetylglucosamine enolpyruvyl transferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family. MurA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789580, Length=418, Percent_Identity=80.8612440191388, Blast_Score=692, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001986
- InterPro:   IPR013792
- InterPro:   IPR005750 [H]

Pfam domain/function: PF00275 EPSP_synthase [H]

EC number: =2.5.1.7 [H]

Molecular weight: Translated: 44687; Mature: 44687

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFRVIGSTQPLQGEVTISGAKNAALPILFASILAEEPVEVANVPHLRDIDTTMELLER
CCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHH
LGAKVERNGSVHVDAGPINQYCAPYDLVKTMRASIWALGPLVARFGQGQVSLPGGCAIGA
HCCCEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC
RPVDLHIHGLEQLGATITLEDGYVKAHVDGRLQGAHIVMDKVSVGATITIMCAATLAEGT
EEEEEEEECHHHCCCEEEEECCEEEEEECCCCCCCEEEEEECCCCCEEEEEEEHHHHCCC
TVLDNAAREPEIVDTAMFLNKLGAKISGAGTDSITIEGVERLGGGKHAVVPDRIETGTFL
HHHHCCCCCCHHHHHHHHHHHHCCEEECCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCEE
VAAAVSRGKIVCRNTHAHLLEAVLAKLEEAGAEIECGEDWISLDMTGRELKAVTVRTAPH
EEEECCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCEEEEEEEEECCC
PGFPTDMQAQFTLLNMMAKGGGVITETIFENRFMHVPELKRMGAKAEIEGNTVICGDVDR
CCCCCCCHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCHHHHCCCCEEECCCEEEECCHHH
LSGAQVMATDLRASASLVIAGCIAKGETIVDRIYHIDRGYERIEDKLSALGANIERFRD
CCCCEEEEHHHHCCCEEEEEEHHHCCHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MEKFRVIGSTQPLQGEVTISGAKNAALPILFASILAEEPVEVANVPHLRDIDTTMELLER
CCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHH
LGAKVERNGSVHVDAGPINQYCAPYDLVKTMRASIWALGPLVARFGQGQVSLPGGCAIGA
HCCCEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC
RPVDLHIHGLEQLGATITLEDGYVKAHVDGRLQGAHIVMDKVSVGATITIMCAATLAEGT
EEEEEEEECHHHCCCEEEEECCEEEEEECCCCCCCEEEEEECCCCCEEEEEEEHHHHCCC
TVLDNAAREPEIVDTAMFLNKLGAKISGAGTDSITIEGVERLGGGKHAVVPDRIETGTFL
HHHHCCCCCCHHHHHHHHHHHHCCEEECCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCEE
VAAAVSRGKIVCRNTHAHLLEAVLAKLEEAGAEIECGEDWISLDMTGRELKAVTVRTAPH
EEEECCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCEEEEEEEEECCC
PGFPTDMQAQFTLLNMMAKGGGVITETIFENRFMHVPELKRMGAKAEIEGNTVICGDVDR
CCCCCCCHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCHHHHCCCCEEECCCEEEECCHHH
LSGAQVMATDLRASASLVIAGCIAKGETIVDRIYHIDRGYERIEDKLSALGANIERFRD
CCCCEEEEHHHHCCCEEEEEEHHHCCHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA