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The map label for this gene is dosC [H]

Identifier: 147674222

GI number: 147674222

Start: 2190550

End: 2191842

Strand: Reverse

Name: dosC [H]

Synonym: VC0395_A2031

Alternate gene names: 147674222

Gene position: 2191842-2190550 (Counterclockwise)

Preceding gene: 147673797

Following gene: 147674864

Centisome position: 72.48

GC content: 47.33

Gene sequence:

>1293_bases
ATGACTGATCAAACGCGCACTTCGCCACTGGTTAATCGCTTACTAAAGATGATTTCACTGTGTGCGCTTGGGTATTTGCT
GATCATTTTGGCCATCATTATTCCACGCGGCATACAGAGTGTTGAGCAGCAAAAACAAGAGCTGGAAGAGAAGCTTGCGC
TCTCCTTGAGTAACTCGGCGGCCATTGCACTGTATGTGAATAATTACGATATTGCCTCCGAGGTGATGGATGCGCTGCTG
CTCCATCAAGAGATCAATGCGGTTAAGTTAGCCAGCGTGGATGGCATTGTCTTTGAACGAACCACCATGCCCACAACCTA
CAAAGAACAGAATTATTGGAGTGATGCGAACCGCTACCGATTAGATTCTCCCGTAGATGGTAATTTGATTGGCTATCTGA
TGATTCATGAGGATCATCAGGTAATTCGTCAGCAAGCCATTAATCAGATTCTTGATCAGATAGCCGTGGTTTTGATCCAG
TTTCTCGTGACTTTTATTGCGCTTATCTGGATCGTTCGCCGCTTGGTCGGTAAACCCCTGACGGATCTTTCACAGGCGTT
ATCCGAAGTGCGGCCAGATCATGATCGCAAAGTGGCGGTAGCCGCAGAAGATCACCACAACGAGATTGGTTTGGTAGCCA
AAAGCATCAATGAATTTATCGACGCTTCCCATCAAGCCTTGCTGCGTGAGCGCGAGCTGCGTCAACAGATTGAACGTTGG
GAAAGCTACTATCGCACCATTGCCGAACAAGACACTCTGACGGGACTAAAAAACCGTTTAGGTTGTGAGAAGTTCGTGCT
CCGCAAACAGCGTGCTAGCACAACCATGGTACTGCTATTGATTGATTTGGATGGTTTTAAGCAGGTGAATGATACCTTGG
GGCATGCGGCTGGTGATGAGGTATTACGTGAAATCGCTAAGCGTTTTTATGCCTTGGCGCAAACGCACTTTTCTGATTTT
GTGGTCGGACGGCTCGGTGGCGATGAGTTTGCTATTTACATTCCGCTTGATGAATTTGTTGCCGAATCTATCGAGCGGTT
TGCGGCTCAGTTGATCGAATCCTCCTCTGAGCCGATTGCATTGGGTCAGCAGAGTGCGCAAGTGGGTTGCAGTATCGGGA
TTAGTCATATGAACAGTCTGCACATTGACTTAGAAAAACTGCTGCTACAAGCCGATAAAGCCATGTACTGGGTCAAATAC
AGAGGCAAACATGGCTATCATATTTATCGAATGGGGGAAGAGATCCTGCCTCTTCCCAGTGCGAAAGAACTCAAGCAGGA
TCAGTTCAGATAG

Upstream 100 bases:

>100_bases
AGTGAACAAGAGCTGATTGCCAAATTAGAAGAGAATCCGTTCTCGATTGGCTATACCGATCAAGTGCCCAATGACGCGCT
GAAAGTATTACTGGTGCTCT

Downstream 100 bases:

>100_bases
TCGCGAGAGGCTTCGAGCACTTTCGCCATCTCATCTTGCAGCTCAAATAACTCAGGCTCTAACTCATCGATGCTCGCCCC
ATTGCGCAGCGCTTGCTCCA

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTDQTRTSPLVNRLLKMISLCALGYLLIILAIIIPRGIQSVEQQKQELEEKLALSLSNSAAIALYVNNYDIASEVMDALL
LHQEINAVKLASVDGIVFERTTMPTTYKEQNYWSDANRYRLDSPVDGNLIGYLMIHEDHQVIRQQAINQILDQIAVVLIQ
FLVTFIALIWIVRRLVGKPLTDLSQALSEVRPDHDRKVAVAAEDHHNEIGLVAKSINEFIDASHQALLRERELRQQIERW
ESYYRTIAEQDTLTGLKNRLGCEKFVLRKQRASTTMVLLLIDLDGFKQVNDTLGHAAGDEVLREIAKRFYALAQTHFSDF
VVGRLGGDEFAIYIPLDEFVAESIERFAAQLIESSSEPIALGQQSAQVGCSIGISHMNSLHIDLEKLLLQADKAMYWVKY
RGKHGYHIYRMGEEILPLPSAKELKQDQFR

Sequences:

>Translated_430_residues
MTDQTRTSPLVNRLLKMISLCALGYLLIILAIIIPRGIQSVEQQKQELEEKLALSLSNSAAIALYVNNYDIASEVMDALL
LHQEINAVKLASVDGIVFERTTMPTTYKEQNYWSDANRYRLDSPVDGNLIGYLMIHEDHQVIRQQAINQILDQIAVVLIQ
FLVTFIALIWIVRRLVGKPLTDLSQALSEVRPDHDRKVAVAAEDHHNEIGLVAKSINEFIDASHQALLRERELRQQIERW
ESYYRTIAEQDTLTGLKNRLGCEKFVLRKQRASTTMVLLLIDLDGFKQVNDTLGHAAGDEVLREIAKRFYALAQTHFSDF
VVGRLGGDEFAIYIPLDEFVAESIERFAAQLIESSSEPIALGQQSAQVGCSIGISHMNSLHIDLEKLLLQADKAMYWVKY
RGKHGYHIYRMGEEILPLPSAKELKQDQFR
>Mature_429_residues
TDQTRTSPLVNRLLKMISLCALGYLLIILAIIIPRGIQSVEQQKQELEEKLALSLSNSAAIALYVNNYDIASEVMDALLL
HQEINAVKLASVDGIVFERTTMPTTYKEQNYWSDANRYRLDSPVDGNLIGYLMIHEDHQVIRQQAINQILDQIAVVLIQF
LVTFIALIWIVRRLVGKPLTDLSQALSEVRPDHDRKVAVAAEDHHNEIGLVAKSINEFIDASHQALLRERELRQQIERWE
SYYRTIAEQDTLTGLKNRLGCEKFVLRKQRASTTMVLLLIDLDGFKQVNDTLGHAAGDEVLREIAKRFYALAQTHFSDFV
VGRLGGDEFAIYIPLDEFVAESIERFAAQLIESSSEPIALGQQSAQVGCSIGISHMNSLHIDLEKLLLQADKAMYWVKYR
GKHGYHIYRMGEEILPLPSAKELKQDQFR

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=187, Percent_Identity=33.6898395721925, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788381, Length=167, Percent_Identity=35.9281437125749, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788956, Length=424, Percent_Identity=26.4150943396226, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787541, Length=174, Percent_Identity=28.735632183908, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787802, Length=178, Percent_Identity=27.5280898876405, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786584, Length=163, Percent_Identity=30.0613496932515, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 48840; Mature: 48709

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQTRTSPLVNRLLKMISLCALGYLLIILAIIIPRGIQSVEQQKQELEEKLALSLSNSA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
AIALYVNNYDIASEVMDALLLHQEINAVKLASVDGIVFERTTMPTTYKEQNYWSDANRYR
EEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCCCCCCCCCCCCCCCCCEE
LDSPVDGNLIGYLMIHEDHQVIRQQAINQILDQIAVVLIQFLVTFIALIWIVRRLVGKPL
CCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TDLSQALSEVRPDHDRKVAVAAEDHHNEIGLVAKSINEFIDASHQALLRERELRQQIERW
HHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESYYRTIAEQDTLTGLKNRLGCEKFVLRKQRASTTMVLLLIDLDGFKQVNDTLGHAAGDE
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCHHH
VLREIAKRFYALAQTHFSDFVVGRLGGDEFAIYIPLDEFVAESIERFAAQLIESSSEPIA
HHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCEE
LGQQSAQVGCSIGISHMNSLHIDLEKLLLQADKAMYWVKYRGKHGYHIYRMGEEILPLPS
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCHHCCCCC
AKELKQDQFR
HHHHHHHCCC
>Mature Secondary Structure 
TDQTRTSPLVNRLLKMISLCALGYLLIILAIIIPRGIQSVEQQKQELEEKLALSLSNSA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
AIALYVNNYDIASEVMDALLLHQEINAVKLASVDGIVFERTTMPTTYKEQNYWSDANRYR
EEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCCCCCCCCCCCCCCCCCEE
LDSPVDGNLIGYLMIHEDHQVIRQQAINQILDQIAVVLIQFLVTFIALIWIVRRLVGKPL
CCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TDLSQALSEVRPDHDRKVAVAAEDHHNEIGLVAKSINEFIDASHQALLRERELRQQIERW
HHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESYYRTIAEQDTLTGLKNRLGCEKFVLRKQRASTTMVLLLIDLDGFKQVNDTLGHAAGDE
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCHHH
VLREIAKRFYALAQTHFSDFVVGRLGGDEFAIYIPLDEFVAESIERFAAQLIESSSEPIA
HHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCEE
LGQQSAQVGCSIGISHMNSLHIDLEKLLLQADKAMYWVKYRGKHGYHIYRMGEEILPLPS
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCHHCCCCC
AKELKQDQFR
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]