Click here to switch to the map view.

The map label for this gene is rumA

Identifier: 147674864

GI number: 147674864

Start: 2186285

End: 2187607

Strand: Reverse

Name: rumA

Synonym: VC0395_A2030

Alternate gene names: 147674864

Gene position: 2187607-2186285 (Counterclockwise)

Preceding gene: 147674222

Following gene: 147673921

Centisome position: 72.34

GC content: 50.57

Gene sequence:

>1323_bases
ATGGCACGTTTCTTTCAACCGAAAAAGCACAGCACGTTAGACACAAAGCATCAGCCGGTCACTATTGAACGGTTGGATCA
CCAAGGCAGCGGACTGGCCTTTTTACACAAGAAACCCTTGTTTGTTGACGGCGCTTTGCCGGGGGAAGAGGTGCTGATTC
AGCTCACTGAAAACAAGAGCAAATACGCGCGCGGTCAACTGATTAAAGTGTTGAAACCCAGCGCTGAGCGTGTTGCACCT
TTCTGTGCGCATTATGCGCAATGTGGTGGTTGTGATTTACAGCATCTTGACCGAGCGGGACAAATCCATCACAAGCAGCA
AGCCTTAAGTCAATTAATGGTGAAGTTTGCAGGGCAGAGTCTTGCTCTGTCTGCGCCAGTTTGTTCTGATGATCAAGGCT
ACCGTCGTCGCGCACGCTTAAGCCTGATGTGGGATAAGAAAACTCAGCAACTACAATTGGGTTTTCGCCGCAAGCAGAGC
AAAGCGATTGTTAATGTGACCGATTGCCCCGTGCTGGAGCCGAGTTTAAATGCGCTGTTGCCTGATTTGAACGCTTTGTT
GAGTGAGTGGAGTCAACCTGAGCGGTTGGGGCATGTCGAATTGGTGAAAGGGGATAACACTCGCGTGTTGGTGCTGCGCC
ATTTAGGCGCGCTGATTGAGCAAGATCAGCAGCGTTTGACTGACTTTGCTTCGCAAAACCAACTAACCTTATATCTGATG
CTGGAAGCCGGCGAGTTGCAACATGTGCAAGGTGAAGCGCCATACTGTGAAGAAACAGGCAGCCGCTTAAGTTTCTTGCC
CAGCCATTTTATTCAGGTCAATCGCGCGGTGAATCAGCACATGGTCGCGCAAGCCTTGAATTGGCTTGAGGTTAGCCCAC
AAGAACACGTGCTCGATCTCTTCTGTGGCTTAGGAAATTTCACGCTGCCGCTGGCCAAACAAGCGCAAGCGGTGGTGGGT
GTTGAAGGTGTTGATGAGATGGTGCAACACGCCACTCATAACGCGAAATTGAACCAAATCAATAATGTGGCGTTTTATCA
GGCCAATTTGGAACAAGATATGACGAGCGCCTCATGGGCGCAGCAGAAATTTGCTAAAGTACTGCTCGACCCAGCCCGCG
CAGGCGCAGAAGGGATTGTGGATCAACTCTCTGCGTTAGGTGCCAAGCGGGTTGTGTACGTGTCGTGTAACCCTGCGACA
TTAGCACGAGACAGTCAAAGCTTGCTGAGCCAAGGATTTCGCTTAGAGAAACTGGGGATGCTCGATATGTTCCCGCACAC
CAGTCATCTCGAGTCCATGGCTTTGTTTGTGAAAAAGGGCTAG

Upstream 100 bases:

>100_bases
GCGTTAGCTTAATCAACTCCCTCATCTTCGACAATCTCCCCCGTGCAGAATAGTGATGCCGGGGCGAAAACAAATCTATT
TCAACACTTGAGCACAGAGC

Downstream 100 bases:

>100_bases
CGATTGAACTCCTGATACGAGCGTCAGTCGAAGGCTAAAAATACAATTATTAGGACGATATCAATGGTTGCGGTACGAAG
CGCACACTTAAACCCAGACC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MARFFQPKKHSTLDTKHQPVTIERLDHQGSGLAFLHKKPLFVDGALPGEEVLIQLTENKSKYARGQLIKVLKPSAERVAP
FCAHYAQCGGCDLQHLDRAGQIHHKQQALSQLMVKFAGQSLALSAPVCSDDQGYRRRARLSLMWDKKTQQLQLGFRRKQS
KAIVNVTDCPVLEPSLNALLPDLNALLSEWSQPERLGHVELVKGDNTRVLVLRHLGALIEQDQQRLTDFASQNQLTLYLM
LEAGELQHVQGEAPYCEETGSRLSFLPSHFIQVNRAVNQHMVAQALNWLEVSPQEHVLDLFCGLGNFTLPLAKQAQAVVG
VEGVDEMVQHATHNAKLNQINNVAFYQANLEQDMTSASWAQQKFAKVLLDPARAGAEGIVDQLSALGAKRVVYVSCNPAT
LARDSQSLLSQGFRLEKLGMLDMFPHTSHLESMALFVKKG

Sequences:

>Translated_440_residues
MARFFQPKKHSTLDTKHQPVTIERLDHQGSGLAFLHKKPLFVDGALPGEEVLIQLTENKSKYARGQLIKVLKPSAERVAP
FCAHYAQCGGCDLQHLDRAGQIHHKQQALSQLMVKFAGQSLALSAPVCSDDQGYRRRARLSLMWDKKTQQLQLGFRRKQS
KAIVNVTDCPVLEPSLNALLPDLNALLSEWSQPERLGHVELVKGDNTRVLVLRHLGALIEQDQQRLTDFASQNQLTLYLM
LEAGELQHVQGEAPYCEETGSRLSFLPSHFIQVNRAVNQHMVAQALNWLEVSPQEHVLDLFCGLGNFTLPLAKQAQAVVG
VEGVDEMVQHATHNAKLNQINNVAFYQANLEQDMTSASWAQQKFAKVLLDPARAGAEGIVDQLSALGAKRVVYVSCNPAT
LARDSQSLLSQGFRLEKLGMLDMFPHTSHLESMALFVKKG
>Mature_439_residues
ARFFQPKKHSTLDTKHQPVTIERLDHQGSGLAFLHKKPLFVDGALPGEEVLIQLTENKSKYARGQLIKVLKPSAERVAPF
CAHYAQCGGCDLQHLDRAGQIHHKQQALSQLMVKFAGQSLALSAPVCSDDQGYRRRARLSLMWDKKTQQLQLGFRRKQSK
AIVNVTDCPVLEPSLNALLPDLNALLSEWSQPERLGHVELVKGDNTRVLVLRHLGALIEQDQQRLTDFASQNQLTLYLML
EAGELQHVQGEAPYCEETGSRLSFLPSHFIQVNRAVNQHMVAQALNWLEVSPQEHVLDLFCGLGNFTLPLAKQAQAVVGV
EGVDEMVQHATHNAKLNQINNVAFYQANLEQDMTSASWAQQKFAKVLLDPARAGAEGIVDQLSALGAKRVVYVSCNPATL
ARDSQSLLSQGFRLEKLGMLDMFPHTSHLESMALFVKKG

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=203, Percent_Identity=32.512315270936, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI51173878, Length=203, Percent_Identity=32.512315270936, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI269784680, Length=208, Percent_Identity=25.9615384615385, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI269784676, Length=208, Percent_Identity=25.9615384615385, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI20149688, Length=208, Percent_Identity=25.9615384615385, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI269784678, Length=208, Percent_Identity=25.9615384615385, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1789148, Length=438, Percent_Identity=48.6301369863014, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1787083, Length=384, Percent_Identity=27.6041666666667, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790403, Length=183, Percent_Identity=31.1475409836066, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24666579, Length=218, Percent_Identity=30.2752293577982, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_VIBC3 (A5F5I8)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F5I8
- SMR:   A5F5I8
- STRING:   A5F5I8
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2030
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   TFERIVY
- ProtClustDB:   PRK13168
- BioCyc:   VCHO345073:VC0395_A2030-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 48940; Mature: 48809

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 396-396 BINDING 272-272 BINDING 301-301 BINDING 306-306 BINDING 322-322 BINDING 349-349 BINDING 370-370

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFFQPKKHSTLDTKHQPVTIERLDHQGSGLAFLHKKPLFVDGALPGEEVLIQLTENKS
CCCCCCCCCCCCCCCCCCCEEHEECCCCCCCEEEEECCCEEEECCCCCHHEEEEECCCCC
KYARGQLIKVLKPSAERVAPFCAHYAQCGGCDLQHLDRAGQIHHKQQALSQLMVKFAGQS
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LALSAPVCSDDQGYRRRARLSLMWDKKTQQLQLGFRRKQSKAIVNVTDCPVLEPSLNALL
EEEECCCCCCCHHHHHHHHEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
PDLNALLSEWSQPERLGHVELVKGDNTRVLVLRHLGALIEQDQQRLTDFASQNQLTLYLM
HHHHHHHHCCCCCHHCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
LEAGELQHVQGEAPYCEETGSRLSFLPSHFIQVNRAVNQHMVAQALNWLEVSPQEHVLDL
EECCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FCGLGNFTLPLAKQAQAVVGVEGVDEMVQHATHNAKLNQINNVAFYQANLEQDMTSASWA
HHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCEEEEECCHHHHHHHHHHH
QQKFAKVLLDPARAGAEGIVDQLSALGAKRVVYVSCNPATLARDSQSLLSQGFRLEKLGM
HHHHHHHHHCHHHCCHHHHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHCCCCEECCCC
LDMFPHTSHLESMALFVKKG
HHCCCCHHHHHHHHHHEECC
>Mature Secondary Structure 
ARFFQPKKHSTLDTKHQPVTIERLDHQGSGLAFLHKKPLFVDGALPGEEVLIQLTENKS
CCCCCCCCCCCCCCCCCCEEHEECCCCCCCEEEEECCCEEEECCCCCHHEEEEECCCCC
KYARGQLIKVLKPSAERVAPFCAHYAQCGGCDLQHLDRAGQIHHKQQALSQLMVKFAGQS
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LALSAPVCSDDQGYRRRARLSLMWDKKTQQLQLGFRRKQSKAIVNVTDCPVLEPSLNALL
EEEECCCCCCCHHHHHHHHEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
PDLNALLSEWSQPERLGHVELVKGDNTRVLVLRHLGALIEQDQQRLTDFASQNQLTLYLM
HHHHHHHHCCCCCHHCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
LEAGELQHVQGEAPYCEETGSRLSFLPSHFIQVNRAVNQHMVAQALNWLEVSPQEHVLDL
EECCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FCGLGNFTLPLAKQAQAVVGVEGVDEMVQHATHNAKLNQINNVAFYQANLEQDMTSASWA
HHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCEEEEECCHHHHHHHHHHH
QQKFAKVLLDPARAGAEGIVDQLSALGAKRVVYVSCNPATLARDSQSLLSQGFRLEKLGM
HHHHHHHHHCHHHCCHHHHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHCCCCEECCCC
LDMFPHTSHLESMALFVKKG
HHCCCCHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA