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The map label for this gene is stcE [H]

Identifier: 147674173

GI number: 147674173

Start: 484366

End: 487239

Strand: Direct

Name: stcE [H]

Synonym: VC0395_A0455

Alternate gene names: 147674173

Gene position: 484366-487239 (Clockwise)

Preceding gene: 147673097

Following gene: 147673705

Centisome position: 16.02

GC content: 47.15

Gene sequence:

>2874_bases
ATGACGTCTCACTATATTGCACTTGCTGTCGGTCTTTTGTCGTTGAGTAGTAACGTGGTTCAAGCCACAACGAATGAAGC
CGAAGGGTGTATTATCAGCCGCCTGAATGGCGAAAAATATTGTTTAAAAGTGGGAGAGCGCTCGGGCTATTCCCTCCCTA
GCTGGATTTATGCTCATCCAGTAGATGTTCAGGCTCCGAGTGGTGTCTCTGTCATGCTAAGTGATTGGGATAACCTGTCT
TATAACCGTTTGGCGGTGTTCGATCGTTATACTGGTAACGAAGATCTTAAGAATGTCAAAGCCTACAACGGTGCTTATTT
AGATTTTTCTAAACCACGTTCAATGCGAGTACTGGCTTCTGAAACTTATCCTGAGGCTTGTATTGTGAGTCGTCAAACGG
GTGAACGTTTCTGTTTAAAAGAGGGTGAGCGCTCTGGTTACTCGCTACCAGCGTACATTTATGGCCATGAGGTTGATGTT
GAAGCACCTCTAGGTTTGGGGGTCATGCTGAGTGATTGGGATAACCTTTCATACAACCGTCTTGCGGTGTTTGGTGGTAA
CACCCAGAACGAGCAAATGCGTGCAGTCAAAGCCTACAATGGCGAAACGCTTGATTTCTCAAAGCCACGCTCAATGCGCG
TTGTCCCTTATGACGGTGACAGCTCGGCACTCAATATGAAGCTTAAGTGGTCTTGGCAAGGCAGTGCTTTCCAACCTAAC
TCTAACCAAGTGATGGTAACGCCTATTGTCGCGCAACTGAACGATGACAATGGTGATGGCAAGATTGATGAAAAAGATGT
CGCAGATTTGATCGTAGTGACGTTTGAAGGAAACAAATACGCCAATGGCGGCTTAGTGCGTGCTTTAAGTGGTGTGGATG
GTTCTGAGCTATGGAGCTATGCGAATGGTGGTGTGATCGCGGATGCGCGTTATTCGCCAGCGGTAGGCGATCTGGACGGT
GACGGAATCGTTGAGATTGTGACTACCAACAATCGCGATCAATTCATCACGATTTTGGATAACCAAGGCAACATTAAAAA
ACAGATCCCAACGACAGAAAGTGGCTGGCGTATTGTCGGTGATATCACGTTGGCGGATTTAGACCACGATGGCAGTGTAG
AAATTCTGGCTGCAGATGGTGTGTATAACTACCACAGCGGATTGGTCTTTAATCATCCTTGGGCGCCGTCCTCGATCAAC
GTTGATGTCGATGGCGATCAGCAACAAGAAGTTTTTTCGGGTGGTACTCTGTTTCAAAATAACGGTGCGATCAATTGGCA
GTATCAAGCGAACGATGCGGTTTGGTTCTCTTCGCTAGTTAACTTAGATAATGATGCTGAGCCTGAGATTGTGGCCTCTG
TACCTGCCACTTTTGCGACAGGCGATAACGCACGTTTTGCTGTGCTAGAACACGATGGCACCATTAAATGGGAAATCAAC
AACACAGCAAACCCTGGTGGTGGTGTGCAGGCGGTATCTAACTTTTTAGGTAAAGCTCAAGCCGTTGAAACGAGTGAGTT
CTCAAAAGTCTATGGTTATCAGCCTAATAACAATCCTGCTTCGATTGCTTTGGCCGTCGATGGCAAGATCTCGGTTCGTT
CAGGATTTGCGATTGATGCGATTGGTGCTTCTGCTTCGACGTTAGTCGGTGGTACGGGAGGCAATCTTAATGCTGCGGTT
AACGTCAAAGATATTAAGGCGATTGACCTGACTTGGGGTAAATACTATTGGGGTGGTTATCATCTATTAGCGCTCGATTT
TAGAATGAGCAATGGTAGCGTGATTTCTATGGGCTCTAAAAACTATGCTTACTCCAAGCAGACCGAGCGCTTTACTGTGC
CAGCAGGTAGCCGCATTAAAGGCATCAAAGCTTGGACCGCGGGTTGGTTGTTGGACGGTGTTCAATTTGAGTTAGCTACG
CAAAATGGTACCAATGATCTGGATGTAAAAGGGATTGTTTACGCAGGCTATGCCGCTGTCGATATGTATAACAGCAAAGG
CGAGCGTGTTTGGTCTGTGGCGAATGACGACACTGGCAGCGGTAAAATTGGTGTCTCTGCGTATGACTTTGATAACGATG
GTATCGATGAAGTACTCGTGCAAGATCATGCACGCGTGCGTGTATTAGATGGCAAAACGGGCAAAGAACGTGCGTCGCTA
GCGCACTCCACGGCAACACTTTGGGAATACCCTATCGTGGTCGATCTGGAAGGTGATAACAACGCAGAGTTGATCGTGGC
AGCGAACGATTTCGATCGCCAATATTCGATTAACCACGGGGTTTATGTTTACCAATCAGCGGACAGTTCGAAACCTTGGA
AGAATGCGACTCGTATTTGGAACCAGCATGCTTTCCATCTCACCAACATCAATCAAGATGGTACTTTGCCGACTTTTGTT
GAGCCAAGTTGGTTGAGCCATAATACCTACCGTTCAAGCACATTACGCGCTGCAGTGGGTGGTGAGTCTCCAATTTTCGG
TTACTCAAATACTCAACAATCGCAACGTGTCGTGACGGCGGATAACCAGATGTATCTGCGTTCAGGTTTTGCGATCGATG
CCATTGGTACTACCGTCAACAACTTAGTCGGCGGGCCGGTGCAAGGCACTAACGGTGGTGTGTTAAGAGCACCGATTGCA
CTTGATCAATTGCAATCAGTGGAGGTCACCTCTGGCCTTTATAACTGGGGTGGTTACCACATCGTGGCGATTAAATTCAC
GATGAAGGATGGCTCTAGCGTGTTATTGGGCTCAACCCACTATGCGAGCAATAAGAAAGTGGAAACATACACTGTGCCGC
AAGGTAAACGTATCAAGCAGATTAATGTATGGACCGGCGGTTGGTTAGTTGAAGGCTTCCAGTTTGTCTACTAA

Upstream 100 bases:

>100_bases
CCAGTTCCTAGGTTTTAAAATAATAATTACAACGATCAACCGGATGCATATTGTTTCTGGCTTTGAATAAAATTAACACA
AGGTAAAGGGAGTCTTACAA

Downstream 100 bases:

>100_bases
CGACTCATCGCTTTACTGAACTGCGAATAAAAACACCCCGATGCTTCGGGGTGTTTTTTTATTGTCATCCCTAAACTCCG
CTTTGAACGTAAGTGATTGT

Product: hypothetical protein

Products: NA

Alternate protein names: Mucinase; Neutral zinc metalloprotease stcE; Secreted protease of C1 esterase inhibitor from EHEC [H]

Number of amino acids: Translated: 957; Mature: 956

Protein sequence:

>957_residues
MTSHYIALAVGLLSLSSNVVQATTNEAEGCIISRLNGEKYCLKVGERSGYSLPSWIYAHPVDVQAPSGVSVMLSDWDNLS
YNRLAVFDRYTGNEDLKNVKAYNGAYLDFSKPRSMRVLASETYPEACIVSRQTGERFCLKEGERSGYSLPAYIYGHEVDV
EAPLGLGVMLSDWDNLSYNRLAVFGGNTQNEQMRAVKAYNGETLDFSKPRSMRVVPYDGDSSALNMKLKWSWQGSAFQPN
SNQVMVTPIVAQLNDDNGDGKIDEKDVADLIVVTFEGNKYANGGLVRALSGVDGSELWSYANGGVIADARYSPAVGDLDG
DGIVEIVTTNNRDQFITILDNQGNIKKQIPTTESGWRIVGDITLADLDHDGSVEILAADGVYNYHSGLVFNHPWAPSSIN
VDVDGDQQQEVFSGGTLFQNNGAINWQYQANDAVWFSSLVNLDNDAEPEIVASVPATFATGDNARFAVLEHDGTIKWEIN
NTANPGGGVQAVSNFLGKAQAVETSEFSKVYGYQPNNNPASIALAVDGKISVRSGFAIDAIGASASTLVGGTGGNLNAAV
NVKDIKAIDLTWGKYYWGGYHLLALDFRMSNGSVISMGSKNYAYSKQTERFTVPAGSRIKGIKAWTAGWLLDGVQFELAT
QNGTNDLDVKGIVYAGYAAVDMYNSKGERVWSVANDDTGSGKIGVSAYDFDNDGIDEVLVQDHARVRVLDGKTGKERASL
AHSTATLWEYPIVVDLEGDNNAELIVAANDFDRQYSINHGVYVYQSADSSKPWKNATRIWNQHAFHLTNINQDGTLPTFV
EPSWLSHNTYRSSTLRAAVGGESPIFGYSNTQQSQRVVTADNQMYLRSGFAIDAIGTTVNNLVGGPVQGTNGGVLRAPIA
LDQLQSVEVTSGLYNWGGYHIVAIKFTMKDGSSVLLGSTHYASNKKVETYTVPQGKRIKQINVWTGGWLVEGFQFVY

Sequences:

>Translated_957_residues
MTSHYIALAVGLLSLSSNVVQATTNEAEGCIISRLNGEKYCLKVGERSGYSLPSWIYAHPVDVQAPSGVSVMLSDWDNLS
YNRLAVFDRYTGNEDLKNVKAYNGAYLDFSKPRSMRVLASETYPEACIVSRQTGERFCLKEGERSGYSLPAYIYGHEVDV
EAPLGLGVMLSDWDNLSYNRLAVFGGNTQNEQMRAVKAYNGETLDFSKPRSMRVVPYDGDSSALNMKLKWSWQGSAFQPN
SNQVMVTPIVAQLNDDNGDGKIDEKDVADLIVVTFEGNKYANGGLVRALSGVDGSELWSYANGGVIADARYSPAVGDLDG
DGIVEIVTTNNRDQFITILDNQGNIKKQIPTTESGWRIVGDITLADLDHDGSVEILAADGVYNYHSGLVFNHPWAPSSIN
VDVDGDQQQEVFSGGTLFQNNGAINWQYQANDAVWFSSLVNLDNDAEPEIVASVPATFATGDNARFAVLEHDGTIKWEIN
NTANPGGGVQAVSNFLGKAQAVETSEFSKVYGYQPNNNPASIALAVDGKISVRSGFAIDAIGASASTLVGGTGGNLNAAV
NVKDIKAIDLTWGKYYWGGYHLLALDFRMSNGSVISMGSKNYAYSKQTERFTVPAGSRIKGIKAWTAGWLLDGVQFELAT
QNGTNDLDVKGIVYAGYAAVDMYNSKGERVWSVANDDTGSGKIGVSAYDFDNDGIDEVLVQDHARVRVLDGKTGKERASL
AHSTATLWEYPIVVDLEGDNNAELIVAANDFDRQYSINHGVYVYQSADSSKPWKNATRIWNQHAFHLTNINQDGTLPTFV
EPSWLSHNTYRSSTLRAAVGGESPIFGYSNTQQSQRVVTADNQMYLRSGFAIDAIGTTVNNLVGGPVQGTNGGVLRAPIA
LDQLQSVEVTSGLYNWGGYHIVAIKFTMKDGSSVLLGSTHYASNKKVETYTVPQGKRIKQINVWTGGWLVEGFQFVY
>Mature_956_residues
TSHYIALAVGLLSLSSNVVQATTNEAEGCIISRLNGEKYCLKVGERSGYSLPSWIYAHPVDVQAPSGVSVMLSDWDNLSY
NRLAVFDRYTGNEDLKNVKAYNGAYLDFSKPRSMRVLASETYPEACIVSRQTGERFCLKEGERSGYSLPAYIYGHEVDVE
APLGLGVMLSDWDNLSYNRLAVFGGNTQNEQMRAVKAYNGETLDFSKPRSMRVVPYDGDSSALNMKLKWSWQGSAFQPNS
NQVMVTPIVAQLNDDNGDGKIDEKDVADLIVVTFEGNKYANGGLVRALSGVDGSELWSYANGGVIADARYSPAVGDLDGD
GIVEIVTTNNRDQFITILDNQGNIKKQIPTTESGWRIVGDITLADLDHDGSVEILAADGVYNYHSGLVFNHPWAPSSINV
DVDGDQQQEVFSGGTLFQNNGAINWQYQANDAVWFSSLVNLDNDAEPEIVASVPATFATGDNARFAVLEHDGTIKWEINN
TANPGGGVQAVSNFLGKAQAVETSEFSKVYGYQPNNNPASIALAVDGKISVRSGFAIDAIGASASTLVGGTGGNLNAAVN
VKDIKAIDLTWGKYYWGGYHLLALDFRMSNGSVISMGSKNYAYSKQTERFTVPAGSRIKGIKAWTAGWLLDGVQFELATQ
NGTNDLDVKGIVYAGYAAVDMYNSKGERVWSVANDDTGSGKIGVSAYDFDNDGIDEVLVQDHARVRVLDGKTGKERASLA
HSTATLWEYPIVVDLEGDNNAELIVAANDFDRQYSINHGVYVYQSADSSKPWKNATRIWNQHAFHLTNINQDGTLPTFVE
PSWLSHNTYRSSTLRAAVGGESPIFGYSNTQQSQRVVTADNQMYLRSGFAIDAIGTTVNNLVGGPVQGTNGGVLRAPIAL
DQLQSVEVTSGLYNWGGYHIVAIKFTMKDGSSVLLGSTHYASNKKVETYTVPQGKRIKQINVWTGGWLVEGFQFVY

Specific function: Virulence factor that contributes to intimate adherence of enterohemorrhagic E.coli (EHEC) O157:H7 to host cells. Is able to cleave the secreted human mucin 7 (MUC7) and the glycoprotein 340 (DMBT1/GP340). Also cleaves human C1 inhibitor (SERPING1), a reg

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Secreted via the etp type II secretion pathway [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase M66 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019503 [H]

Pfam domain/function: PF10462 Peptidase_M66 [H]

EC number: NA

Molecular weight: Translated: 104005; Mature: 103874

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHYIALAVGLLSLSSNVVQATTNEAEGCIISRLNGEKYCLKVGERSGYSLPSWIYAHP
CCCCEEEHHHHHHHHCCCEEEEECCCCCCEEEEECCCCEEEEEECCCCCCCCCCEEEECC
VDVQAPSGVSVMLSDWDNLSYNRLAVFDRYTGNEDLKNVKAYNGAYLDFSKPRSMRVLAS
CCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHCCCEEEECCCCCCEEEEEC
ETYPEACIVSRQTGERFCLKEGERSGYSLPAYIYGHEVDVEAPLGLGVMLSDWDNLSYNR
CCCCCEEEEECCCCCEEHHCCCCCCCCCCEEEEEEEEEEEECCCCCEEEEECCCCCCCCE
LAVFGGNTQNEQMRAVKAYNGETLDFSKPRSMRVVPYDGDSSALNMKLKWSWQGSAFQPN
EEEECCCCCCCCEEEEEECCCCEEECCCCCCEEEEEECCCCCEEEEEEEEEECCCCCCCC
SNQVMVTPIVAQLNDDNGDGKIDEKDVADLIVVTFEGNKYANGGLVRALSGVDGSELWSY
CCCEEEEEEEEEECCCCCCCCCCCCCCEEEEEEEEECCEECCCCEEEEECCCCHHHHHHH
ANGGVIADARYSPAVGDLDGDGIVEIVTTNNRDQFITILDNQGNIKKQIPTTESGWRIVG
CCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCEEEECCCCCCCCEEEE
DITLADLDHDGSVEILAADGVYNYHSGLVFNHPWAPSSINVDVDGDQQQEVFSGGTLFQN
EEEEEECCCCCCEEEEEECCEEECCCCEEEECCCCCCEEEEEECCCCHHHHHCCCCEEEC
NGAINWQYQANDAVWFSSLVNLDNDAEPEIVASVPATFATGDNARFAVLEHDGTIKWEIN
CCEEEEEEECCCCEEEHHHHCCCCCCCCCEEEECCCEEECCCCCEEEEEECCCEEEEEEC
NTANPGGGVQAVSNFLGKAQAVETSEFSKVYGYQPNNNPASIALAVDGKISVRSGFAIDA
CCCCCCCHHHHHHHHHCCCCEECCCCHHHHCCCCCCCCCEEEEEEECCEEEEECCCEEEE
IGASASTLVGGTGGNLNAAVNVKDIKAIDLTWGKYYWGGYHLLALDFRMSNGSVISMGSK
CCCCCEEEEECCCCCCEEEEEEEEEEEEEEECCCEEECCEEEEEEEEEECCCCEEEECCC
NYAYSKQTERFTVPAGSRIKGIKAWTAGWLLDGVQFELATQNGTNDLDVKGIVYAGYAAV
CCCCCCCCCEEECCCCCCEEEEEEEECCEEECCEEEEEEECCCCCCCCEEEEEEECEEEE
DMYNSKGERVWSVANDDTGSGKIGVSAYDFDNDGIDEVLVQDHARVRVLDGKTGKERASL
EEECCCCCEEEEECCCCCCCCEEEEEEEECCCCCHHHHHHCCCCEEEEEECCCCCHHHHH
AHSTATLWEYPIVVDLEGDNNAELIVAANDFDRQYSINHGVYVYQSADSSKPWKNATRIW
HHCCCEEEECCEEEEEECCCCCEEEEEECCCCCEEECCCCEEEEECCCCCCCCHHHHHHH
NQHAFHLTNINQDGTLPTFVEPSWLSHNTYRSSTLRAAVGGESPIFGYSNTQQSQRVVTA
CCCEEEEEECCCCCCCCCEECCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCEEEEE
DNQMYLRSGFAIDAIGTTVNNLVGGPVQGTNGGVLRAPIALDQLQSVEVTSGLYNWGGYH
CCEEEEECCCEEEEHHHHHHHHCCCCCCCCCCCEEECCEEHHHHCCEEECCCEECCCCEE
IVAIKFTMKDGSSVLLGSTHYASNKKVETYTVPQGKRIKQINVWTGGWLVEGFQFVY
EEEEEEEECCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEEEECCEEECCEEECC
>Mature Secondary Structure 
TSHYIALAVGLLSLSSNVVQATTNEAEGCIISRLNGEKYCLKVGERSGYSLPSWIYAHP
CCCEEEHHHHHHHHCCCEEEEECCCCCCEEEEECCCCEEEEEECCCCCCCCCCEEEECC
VDVQAPSGVSVMLSDWDNLSYNRLAVFDRYTGNEDLKNVKAYNGAYLDFSKPRSMRVLAS
CCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHCCCEEEECCCCCCEEEEEC
ETYPEACIVSRQTGERFCLKEGERSGYSLPAYIYGHEVDVEAPLGLGVMLSDWDNLSYNR
CCCCCEEEEECCCCCEEHHCCCCCCCCCCEEEEEEEEEEEECCCCCEEEEECCCCCCCCE
LAVFGGNTQNEQMRAVKAYNGETLDFSKPRSMRVVPYDGDSSALNMKLKWSWQGSAFQPN
EEEECCCCCCCCEEEEEECCCCEEECCCCCCEEEEEECCCCCEEEEEEEEEECCCCCCCC
SNQVMVTPIVAQLNDDNGDGKIDEKDVADLIVVTFEGNKYANGGLVRALSGVDGSELWSY
CCCEEEEEEEEEECCCCCCCCCCCCCCEEEEEEEEECCEECCCCEEEEECCCCHHHHHHH
ANGGVIADARYSPAVGDLDGDGIVEIVTTNNRDQFITILDNQGNIKKQIPTTESGWRIVG
CCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCEEEECCCCCCCCEEEE
DITLADLDHDGSVEILAADGVYNYHSGLVFNHPWAPSSINVDVDGDQQQEVFSGGTLFQN
EEEEEECCCCCCEEEEEECCEEECCCCEEEECCCCCCEEEEEECCCCHHHHHCCCCEEEC
NGAINWQYQANDAVWFSSLVNLDNDAEPEIVASVPATFATGDNARFAVLEHDGTIKWEIN
CCEEEEEEECCCCEEEHHHHCCCCCCCCCEEEECCCEEECCCCCEEEEEECCCEEEEEEC
NTANPGGGVQAVSNFLGKAQAVETSEFSKVYGYQPNNNPASIALAVDGKISVRSGFAIDA
CCCCCCCHHHHHHHHHCCCCEECCCCHHHHCCCCCCCCCEEEEEEECCEEEEECCCEEEE
IGASASTLVGGTGGNLNAAVNVKDIKAIDLTWGKYYWGGYHLLALDFRMSNGSVISMGSK
CCCCCEEEEECCCCCCEEEEEEEEEEEEEEECCCEEECCEEEEEEEEEECCCCEEEECCC
NYAYSKQTERFTVPAGSRIKGIKAWTAGWLLDGVQFELATQNGTNDLDVKGIVYAGYAAV
CCCCCCCCCEEECCCCCCEEEEEEEECCEEECCEEEEEEECCCCCCCCEEEEEEECEEEE
DMYNSKGERVWSVANDDTGSGKIGVSAYDFDNDGIDEVLVQDHARVRVLDGKTGKERASL
EEECCCCCEEEEECCCCCCCCEEEEEEEECCCCCHHHHHHCCCCEEEEEECCCCCHHHHH
AHSTATLWEYPIVVDLEGDNNAELIVAANDFDRQYSINHGVYVYQSADSSKPWKNATRIW
HHCCCEEEECCEEEEEECCCCCEEEEEECCCCCEEECCCCEEEEECCCCCCCCHHHHHHH
NQHAFHLTNINQDGTLPTFVEPSWLSHNTYRSSTLRAAVGGESPIFGYSNTQQSQRVVTA
CCCEEEEEECCCCCCCCCEECCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCEEEEE
DNQMYLRSGFAIDAIGTTVNNLVGGPVQGTNGGVLRAPIALDQLQSVEVTSGLYNWGGYH
CCEEEEECCCEEEEHHHHHHHHCCCCCCCCCCCEEECCEEHHHHCCEEECCCEECCCCEE
IVAIKFTMKDGSSVLLGSTHYASNKKVETYTVPQGKRIKQINVWTGGWLVEGFQFVY
EEEEEEEECCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEEEECCEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9722640; 9628576; 12123444 [H]