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The map label for this gene is wcaJ [H]

Identifier: 147673705

GI number: 147673705

Start: 489981

End: 491378

Strand: Direct

Name: wcaJ [H]

Synonym: VC0395_A0458

Alternate gene names: 147673705

Gene position: 489981-491378 (Clockwise)

Preceding gene: 147674173

Following gene: 147675750

Centisome position: 16.2

GC content: 45.06

Gene sequence:

>1398_bases
GTGATGAAGGAAAAAAGCAGAATACGCATTACTAACTATCACGGTAAATTTTTCTACCGATTAATTGATAGCATTTTTAT
ATTAGTAATTATGTATATATCGATTAAGTCGAATCAGCATGTTGTTACCATGTCTTATATTTCGGTTGCCTTGCTAGGGG
TGCTTTTTTACTCCTACATTGCAGAAAGTTTAGATATTTACAGTGGTTGGCGAACAGCAAAGCTGCGTTCACTTTCTTTA
CATACGGCATTCTGTTGGGCTGCGACCATCGCTAGCTTGACCCTATTGGCTTACTTCTCCAAAACGGGGATCGAGTTTTC
TCGCATGGTGATGGGCGCTTGGTTTGTCGGTTCTTTTATTGGCTTGATTGGTTGGCGTGTATTGGCATTTGCGACCATTC
ACTACATGCACAAAAAGGGTCTGCATACCAAGAACGCGGTCATTATCGGTATGACTACTCAAGGGCAAGAGTTATCAAAT
AATCTGCTCAAAAACCCTGAACTAGGGATTGTGATGCAAGGTTTTTATGATGACCGTGCGCCTGAGCGCCTTAAAGAGGG
GGCGCCTGTGCTGGGCAATATCAATGATGCATTAAGCCTTGCGAAAACTGGGCAGGTGCAAAATGTCTATATTGCTTTGC
CGATGCAAGCGCAGCGCCGTATCAACCAAATTTTGGATGCGTTTTCGGACAGTACGGTCAATACCTACATAGTGCCTGAC
TTTTTTACTTTTAATTTACTGCACTCACGTTGGTACACGATTGGTGATGTCAACGCGTTTAGCATTTTTGATACCCCCTT
TAATGGTTTGCTCAACTGGGTGAAACGCTTTGAAGATCTGGTGCTGAGCAGCTTGATTTTGCTCTTGATCAGCCCAGTGT
TATTGGCCGTCGCTATTGGCGTTAAGCTGAGCTCGCCTGGCCCCATCATTTTTAAACAAAACCGCTATGGTTTAGATGGC
AAGCCAATCCAAGTTTGGAAATTTCGCAGTATGCGAGTGATGGATAATGGCAGTCATGTGCAGCAAGCCACCAAAGGCGA
TCCACGAGTTACACGATTTGGTGCGTTCATTCGTCGAACATCGCTGGATGAGTTACCGCAGTTTTTCAATGTGTTGCAAG
GAAGTATGTCGATCGTGGGGCCAAGGCCACATGCCGTTGCGCACAACGAGCAATATCGCACCATAGTGAATCGCTACATG
CTGCGTCACAAAGTGAAGCCCGGCATTACTGGATGGGCACAGATCAATGGTTGGCGTGGTGAAACCGATACGCTCGACAA
GATGGAGAAGCGAGTGCAGTTCGATCTGGACTACATCCATCGCTGGTCGCTGTGGTTTGATCTGAAAATCGTCTTCCTGA
CTATTTTCAAAGGATTTGTTGGAAAAAACGCGTATTAA

Upstream 100 bases:

>100_bases
TGATGCAGATATTTCTATTGATAAAGATGTAGTCTTTAAAAAAGAATTTAATACATTCCAATTATTCTTCGGGAAATCTT
ACATCGGTTAGGAGCGTCTA

Downstream 100 bases:

>100_bases
GACAGGGCACTTGCGTCGTAAGTGCGAGTGCTACTCTTGAATAAATATCGATTTAAATAAGTGCAGGCAAATGGAACCGA
TTAAACTGACTTCTCTTTTT

Product: putative capsular polysaccharide biosynthesis glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MMKEKSRIRITNYHGKFFYRLIDSIFILVIMYISIKSNQHVVTMSYISVALLGVLFYSYIAESLDIYSGWRTAKLRSLSL
HTAFCWAATIASLTLLAYFSKTGIEFSRMVMGAWFVGSFIGLIGWRVLAFATIHYMHKKGLHTKNAVIIGMTTQGQELSN
NLLKNPELGIVMQGFYDDRAPERLKEGAPVLGNINDALSLAKTGQVQNVYIALPMQAQRRINQILDAFSDSTVNTYIVPD
FFTFNLLHSRWYTIGDVNAFSIFDTPFNGLLNWVKRFEDLVLSSLILLLISPVLLAVAIGVKLSSPGPIIFKQNRYGLDG
KPIQVWKFRSMRVMDNGSHVQQATKGDPRVTRFGAFIRRTSLDELPQFFNVLQGSMSIVGPRPHAVAHNEQYRTIVNRYM
LRHKVKPGITGWAQINGWRGETDTLDKMEKRVQFDLDYIHRWSLWFDLKIVFLTIFKGFVGKNAY

Sequences:

>Translated_465_residues
MMKEKSRIRITNYHGKFFYRLIDSIFILVIMYISIKSNQHVVTMSYISVALLGVLFYSYIAESLDIYSGWRTAKLRSLSL
HTAFCWAATIASLTLLAYFSKTGIEFSRMVMGAWFVGSFIGLIGWRVLAFATIHYMHKKGLHTKNAVIIGMTTQGQELSN
NLLKNPELGIVMQGFYDDRAPERLKEGAPVLGNINDALSLAKTGQVQNVYIALPMQAQRRINQILDAFSDSTVNTYIVPD
FFTFNLLHSRWYTIGDVNAFSIFDTPFNGLLNWVKRFEDLVLSSLILLLISPVLLAVAIGVKLSSPGPIIFKQNRYGLDG
KPIQVWKFRSMRVMDNGSHVQQATKGDPRVTRFGAFIRRTSLDELPQFFNVLQGSMSIVGPRPHAVAHNEQYRTIVNRYM
LRHKVKPGITGWAQINGWRGETDTLDKMEKRVQFDLDYIHRWSLWFDLKIVFLTIFKGFVGKNAY
>Mature_465_residues
MMKEKSRIRITNYHGKFFYRLIDSIFILVIMYISIKSNQHVVTMSYISVALLGVLFYSYIAESLDIYSGWRTAKLRSLSL
HTAFCWAATIASLTLLAYFSKTGIEFSRMVMGAWFVGSFIGLIGWRVLAFATIHYMHKKGLHTKNAVIIGMTTQGQELSN
NLLKNPELGIVMQGFYDDRAPERLKEGAPVLGNINDALSLAKTGQVQNVYIALPMQAQRRINQILDAFSDSTVNTYIVPD
FFTFNLLHSRWYTIGDVNAFSIFDTPFNGLLNWVKRFEDLVLSSLILLLISPVLLAVAIGVKLSSPGPIIFKQNRYGLDG
KPIQVWKFRSMRVMDNGSHVQQATKGDPRVTRFGAFIRRTSLDELPQFFNVLQGSMSIVGPRPHAVAHNEQYRTIVNRYM
LRHKVKPGITGWAQINGWRGETDTLDKMEKRVQFDLDYIHRWSLWFDLKIVFLTIFKGFVGKNAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=426, Percent_Identity=48.8262910798122, Blast_Score=414, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 53099; Mature: 53099

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKEKSRIRITNYHGKFFYRLIDSIFILVIMYISIKSNQHVVTMSYISVALLGVLFYSYI
CCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
AESLDIYSGWRTAKLRSLSLHTAFCWAATIASLTLLAYFSKTGIEFSRMVMGAWFVGSFI
HHHHHHHCCCCHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GLIGWRVLAFATIHYMHKKGLHTKNAVIIGMTTQGQELSNNLLKNPELGIVMQGFYDDRA
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEECCCCCCC
PERLKEGAPVLGNINDALSLAKTGQVQNVYIALPMQAQRRINQILDAFSDSTVNTYIVPD
HHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEECCC
FFTFNLLHSRWYTIGDVNAFSIFDTPFNGLLNWVKRFEDLVLSSLILLLISPVLLAVAIG
HHHHHHHHHCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VKLSSPGPIIFKQNRYGLDGKPIQVWKFRSMRVMDNGSHVQQATKGDPRVTRFGAFIRRT
CEECCCCCEEEECCCCCCCCCCEEEEEEECEEEECCCCHHHHHCCCCCCHHHHHHHHHHC
SLDELPQFFNVLQGSMSIVGPRPHAVAHNEQYRTIVNRYMLRHKVKPGITGWAQINGWRG
CHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
ETDTLDKMEKRVQFDLDYIHRWSLWFDLKIVFLTIFKGFVGKNAY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMKEKSRIRITNYHGKFFYRLIDSIFILVIMYISIKSNQHVVTMSYISVALLGVLFYSYI
CCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
AESLDIYSGWRTAKLRSLSLHTAFCWAATIASLTLLAYFSKTGIEFSRMVMGAWFVGSFI
HHHHHHHCCCCHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GLIGWRVLAFATIHYMHKKGLHTKNAVIIGMTTQGQELSNNLLKNPELGIVMQGFYDDRA
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEECCCCCCC
PERLKEGAPVLGNINDALSLAKTGQVQNVYIALPMQAQRRINQILDAFSDSTVNTYIVPD
HHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEECCC
FFTFNLLHSRWYTIGDVNAFSIFDTPFNGLLNWVKRFEDLVLSSLILLLISPVLLAVAIG
HHHHHHHHHCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VKLSSPGPIIFKQNRYGLDGKPIQVWKFRSMRVMDNGSHVQQATKGDPRVTRFGAFIRRT
CEECCCCCEEEECCCCCCCCCCEEEEEEECEEEECCCCHHHHHCCCCCCHHHHHHHHHHC
SLDELPQFFNVLQGSMSIVGPRPHAVAHNEQYRTIVNRYMLRHKVKPGITGWAQINGWRG
CHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
ETDTLDKMEKRVQFDLDYIHRWSLWFDLKIVFLTIFKGFVGKNAY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]