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The map label for this gene is lptD [H]

Identifier: 146342593

GI number: 146342593

Start: 5957057

End: 5959531

Strand: Direct

Name: lptD [H]

Synonym: BRADO5756

Alternate gene names: 146342593

Gene position: 5957057-5959531 (Clockwise)

Preceding gene: 146342592

Following gene: 146342596

Centisome position: 79.89

GC content: 65.41

Gene sequence:

>2475_bases
ATGACGAAGCCTGCCCGCGTGACCGACCGCTGCTTTGCTCGCTCCCGAACGGCGTCGACGGACTCGCGCCGCGTGCGGGC
CCTCAGGAGGAGCCCCGCTGCGCTCGCCGTGTGCGTTGTGCTGGCCGCCGGGGCGGTCCTGCCGGCGACCGCGTACGCCC
AGAGCTTCACCTACAATCCGCTCCCGCCGAAGCCGCCGCCACCGCGCGTCGCCAACGACAACCAGATGCTGGTGCAGGCG
ACCACCGTCGAATACGACGCCAACAATTCGCGCGTCGCGGCGGTCGGCAACGTGCAGCTGTTCTACAACGGCTCCTCGGT
CGAGGCCGACCGGGTGATCTACGACCAGAAGACCAAGCGCCTACATGCGGAAGGCAACATCCGCATGACCGACGCCGACG
GCAAGATCACCTACGCCGACAGCCTCGATCTCTCCGACGACTACCGCGATGGCTTCGTCGATTCCTTGCGCGTCGAGACC
GAGGACCAGACGCGAATGGCCGCGACCCGCGCCGACCGCAGCAAGGGCAACACCACGGTCTTCGAGAACGGCGTCTACAC
CGCCTGCGCGCCGTGCCGGGACGATCCGAAAAAGCCGCCGCTGTGGCAGGTGAAGGGGGCGCGGATCATCCACGACCAGA
CCGAGAAGATGCTGTATTTCGAGAACGCCCAGCTCGAAATGTTCGGCGTCCCGCTCGCCTACATCCCGTATTTCTCGACG
CCCGATCCGACGGTCAAGCGCAAGAGCGGCTTCCTGATGCCGGGCTTCTCGCAGAACAACGCCTATGGCATCGGCGTCGA
CATCCCCTATTACTGGGCGATCGACGGGAGCTACGACGCGACCTTCACGCCGCGCCTCACCTCCAGGCAGGGCGTGCTGG
TTCAGACCGAATTCCGCCAGCGGCTCGACAATGGCAGCTATCAGGTGCGCTTCTACGGCATCGATCAGCTCGATCGCAAC
GCCTTCGCCGGACAGCCGGGCGATCGCCAGCTGCGCGGCGGCATCGACAGCAGGGGCCAGCTCGCGCTGTCGGACAAATG
GACGTTCGGCTGGGACGGCGTCCTGCTGTCCGACTACACGTTCCTGTCGGACTATCGGCTCGTGCAGTACCGCGATCCGT
TCAACGCCTTCATGACTCTGCCGACGGAAGCGATCTCGCAGCTGTATCTGACCGGCGTCGGCAGCCGCAGCGTCTTCGAC
ATCAGGGCGATGCACTATCTGAGCTTCTCGGGCAACCAGCAGACAGTTCCGATCGTCTATCCGGTGCTGGACTATTCAAA
CGTGCTGAACTCTCCCGTGCTCGGCGGCGAGTTCAGCACCAAGACCAACTTCGTCAATCTCACCCGCGAGCAGGCGGTGT
TCGATCCGGTCACCACCACGGCCAACACCAACGGCCTGTGCACGACGGCCTCGGCGGATCCGCTGGCGCGGCTGCCATCG
CAGTGCCTGCTGCGCGCGGCGCCCGGCACCTATACGCGGCTGACCGCGCAGGCGGAGTGGCGGAAGTCGTTCACCGATCC
GATGGGCGAGATCTGGACGCCGTTCGCGATCCTGCGCGCCGACGCGATCAATGCCAACGTAACCGCGCAGCCTGGCGTCG
GCAACTTCCTGCCGACCGGCGATACCCAGGCGTTGCGGGTGACGCCGACGGTCGGCCTGGACTATCGCTACCCGTTCATC
AACGTGCAGTCATGGGGCACCACCACGGTCGAGCCGATCGCGCAGCTCATCGTCCGGCCGAACGAGACCTCGGCCGGCCG
ACTGCCGAACGAGGACGCGCAGAGCCTCGTGTTCGACGCCAGCAATCTGTTCAGCGTCGACAAGTTCTCCGGCTACGACC
GCGTCGCGGGCGGCGGCCGCGCCAATATTGGCGCGCAGGCGACAACGCAGTTCGATCGCGGCGGCACCGTCAATCTGCTG
TTCGGCCAGTCGACCCAGCTGTTCGGGCTGAACTCCTACGCCGTCCAGGACGCCAACAACACCGGTATCGGTTCCGGTCT
CGACAAGACCATGTCCGACTACGTGACGCGGGTGGACTATTCGCCGAACCGGACCTACACACTCAGCATGCGCAGCCGGC
TCGACGAACAGAGCCTCAACGTCCAGCGCTTCGAGGCCGAGGGCAAAGTCAATTTCGACCGCTGGTCCGCGGCGCTGACC
TACGGCAACTACGCGGCCCAGCCGGCGATCGGCAATCTCGACCGGCGCGAGGGCCTGCTCGGCAACGGCTCGGTCAAGGT
GGCGCAGAACTGGGTCGTCTCCGGCGGCGCGCGCTGGGATCTGGTGGCCAACAAGATGAACCAATATCTCGTCGGCGCCG
GCTATGTCGACGACTGCTTCACCTTGGCCGCGAGCTATGTCACCTCGACGAGTTACGCCATCGCCGGCGCGACGCCGCAG
ACCAATCAGACAGTCATGCTGACCTTGGGGCTGCGGACCATCGGCAATGTGTCATTGCCGGTACCGGTCAATTAG

Upstream 100 bases:

>100_bases
AGACGGCACAAGCGCGCCAAAAAAGCCACCAGCGCCGCGCAACATGGATTCGCCTTGCCGCGTCAGCAATCTTTTGCGTG
AATCGATGTCGAAGCGTCCC

Downstream 100 bases:

>100_bases
TCCTGTTTTTCCACTGGAACCAGCTGTTGTTTCTGGGTGCGCTCTCGCCGTCTCCACCACTGTCATGCTCCGCGAAGGCG
GAGCATCCAGTACTCCGCGG

Product: putative organic solvent tolerance protein (OstA)

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 824; Mature: 823

Protein sequence:

>824_residues
MTKPARVTDRCFARSRTASTDSRRVRALRRSPAALAVCVVLAAGAVLPATAYAQSFTYNPLPPKPPPPRVANDNQMLVQA
TTVEYDANNSRVAAVGNVQLFYNGSSVEADRVIYDQKTKRLHAEGNIRMTDADGKITYADSLDLSDDYRDGFVDSLRVET
EDQTRMAATRADRSKGNTTVFENGVYTACAPCRDDPKKPPLWQVKGARIIHDQTEKMLYFENAQLEMFGVPLAYIPYFST
PDPTVKRKSGFLMPGFSQNNAYGIGVDIPYYWAIDGSYDATFTPRLTSRQGVLVQTEFRQRLDNGSYQVRFYGIDQLDRN
AFAGQPGDRQLRGGIDSRGQLALSDKWTFGWDGVLLSDYTFLSDYRLVQYRDPFNAFMTLPTEAISQLYLTGVGSRSVFD
IRAMHYLSFSGNQQTVPIVYPVLDYSNVLNSPVLGGEFSTKTNFVNLTREQAVFDPVTTTANTNGLCTTASADPLARLPS
QCLLRAAPGTYTRLTAQAEWRKSFTDPMGEIWTPFAILRADAINANVTAQPGVGNFLPTGDTQALRVTPTVGLDYRYPFI
NVQSWGTTTVEPIAQLIVRPNETSAGRLPNEDAQSLVFDASNLFSVDKFSGYDRVAGGGRANIGAQATTQFDRGGTVNLL
FGQSTQLFGLNSYAVQDANNTGIGSGLDKTMSDYVTRVDYSPNRTYTLSMRSRLDEQSLNVQRFEAEGKVNFDRWSAALT
YGNYAAQPAIGNLDRREGLLGNGSVKVAQNWVVSGGARWDLVANKMNQYLVGAGYVDDCFTLAASYVTSTSYAIAGATPQ
TNQTVMLTLGLRTIGNVSLPVPVN

Sequences:

>Translated_824_residues
MTKPARVTDRCFARSRTASTDSRRVRALRRSPAALAVCVVLAAGAVLPATAYAQSFTYNPLPPKPPPPRVANDNQMLVQA
TTVEYDANNSRVAAVGNVQLFYNGSSVEADRVIYDQKTKRLHAEGNIRMTDADGKITYADSLDLSDDYRDGFVDSLRVET
EDQTRMAATRADRSKGNTTVFENGVYTACAPCRDDPKKPPLWQVKGARIIHDQTEKMLYFENAQLEMFGVPLAYIPYFST
PDPTVKRKSGFLMPGFSQNNAYGIGVDIPYYWAIDGSYDATFTPRLTSRQGVLVQTEFRQRLDNGSYQVRFYGIDQLDRN
AFAGQPGDRQLRGGIDSRGQLALSDKWTFGWDGVLLSDYTFLSDYRLVQYRDPFNAFMTLPTEAISQLYLTGVGSRSVFD
IRAMHYLSFSGNQQTVPIVYPVLDYSNVLNSPVLGGEFSTKTNFVNLTREQAVFDPVTTTANTNGLCTTASADPLARLPS
QCLLRAAPGTYTRLTAQAEWRKSFTDPMGEIWTPFAILRADAINANVTAQPGVGNFLPTGDTQALRVTPTVGLDYRYPFI
NVQSWGTTTVEPIAQLIVRPNETSAGRLPNEDAQSLVFDASNLFSVDKFSGYDRVAGGGRANIGAQATTQFDRGGTVNLL
FGQSTQLFGLNSYAVQDANNTGIGSGLDKTMSDYVTRVDYSPNRTYTLSMRSRLDEQSLNVQRFEAEGKVNFDRWSAALT
YGNYAAQPAIGNLDRREGLLGNGSVKVAQNWVVSGGARWDLVANKMNQYLVGAGYVDDCFTLAASYVTSTSYAIAGATPQ
TNQTVMLTLGLRTIGNVSLPVPVN
>Mature_823_residues
TKPARVTDRCFARSRTASTDSRRVRALRRSPAALAVCVVLAAGAVLPATAYAQSFTYNPLPPKPPPPRVANDNQMLVQAT
TVEYDANNSRVAAVGNVQLFYNGSSVEADRVIYDQKTKRLHAEGNIRMTDADGKITYADSLDLSDDYRDGFVDSLRVETE
DQTRMAATRADRSKGNTTVFENGVYTACAPCRDDPKKPPLWQVKGARIIHDQTEKMLYFENAQLEMFGVPLAYIPYFSTP
DPTVKRKSGFLMPGFSQNNAYGIGVDIPYYWAIDGSYDATFTPRLTSRQGVLVQTEFRQRLDNGSYQVRFYGIDQLDRNA
FAGQPGDRQLRGGIDSRGQLALSDKWTFGWDGVLLSDYTFLSDYRLVQYRDPFNAFMTLPTEAISQLYLTGVGSRSVFDI
RAMHYLSFSGNQQTVPIVYPVLDYSNVLNSPVLGGEFSTKTNFVNLTREQAVFDPVTTTANTNGLCTTASADPLARLPSQ
CLLRAAPGTYTRLTAQAEWRKSFTDPMGEIWTPFAILRADAINANVTAQPGVGNFLPTGDTQALRVTPTVGLDYRYPFIN
VQSWGTTTVEPIAQLIVRPNETSAGRLPNEDAQSLVFDASNLFSVDKFSGYDRVAGGGRANIGAQATTQFDRGGTVNLLF
GQSTQLFGLNSYAVQDANNTGIGSGLDKTMSDYVTRVDYSPNRTYTLSMRSRLDEQSLNVQRFEAEGKVNFDRWSAALTY
GNYAAQPAIGNLDRREGLLGNGSVKVAQNWVVSGGARWDLVANKMNQYLVGAGYVDDCFTLAASYVTSTSYAIAGATPQT
NQTVMLTLGLRTIGNVSLPVPVN

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=368, Percent_Identity=27.7173913043478, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 90487; Mature: 90356

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPARVTDRCFARSRTASTDSRRVRALRRSPAALAVCVVLAAGAVLPATAYAQSFTYNP
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCC
LPPKPPPPRVANDNQMLVQATTVEYDANNSRVAAVGNVQLFYNGSSVEADRVIYDQKTKR
CCCCCCCCCCCCCCEEEEEEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCEEECCHHHE
LHAEGNIRMTDADGKITYADSLDLSDDYRDGFVDSLRVETEDQTRMAATRADRSKGNTTV
EECCCCEEEECCCCEEEEECCCCCCCCHHCCCCHHEEECCCCHHHHHHHHHHCCCCCCEE
FENGVYTACAPCRDDPKKPPLWQVKGARIIHDQTEKMLYFENAQLEMFGVPLAYIPYFST
EECCCEEEECCCCCCCCCCCEEEECCCEEEECCCCEEEEEECCEEEEECCCCEECCCCCC
PDPTVKRKSGFLMPGFSQNNAYGIGVDIPYYWAIDGSYDATFTPRLTSRQGVLVQTEFRQ
CCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCEECCCCCCCCCEEEEHHHHH
RLDNGSYQVRFYGIDQLDRNAFAGQPGDRQLRGGIDSRGQLALSDKWTFGWDGVLLSDYT
HHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCEEEEECCEECCCCCEEEECEE
FLSDYRLVQYRDPFNAFMTLPTEAISQLYLTGVGSRSVFDIRAMHYLSFSGNQQTVPIVY
EECCEEEEEECCCHHHEEECCHHHHHHEEEECCCCCCEEEEEEEEEEEECCCCEEEEEEE
PVLDYSNVLNSPVLGGEFSTKTNFVNLTREQAVFDPVTTTANTNGLCTTASADPLARLPS
EECCHHHHHCCCCCCCCCCCCCCEEEEEHHHHEECCCCCCCCCCCEEEECCCCHHHHCCH
QCLLRAAPGTYTRLTAQAEWRKSFTDPMGEIWTPFAILRADAINANVTAQPGVGNFLPTG
HHHEECCCCCEEEEEECHHHHHHHCCCHHHHHCCCEEEEECCCCCCEEECCCCCCCCCCC
DTQALRVTPTVGLDYRYPFINVQSWGTTTVEPIAQLIVRPNETSAGRLPNEDAQSLVFDA
CCCEEEEECEECCCEECCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEC
SNLFSVDKFSGYDRVAGGGRANIGAQATTQFDRGGTVNLLFGQSTQLFGLNSYAVQDANN
CCCEEEHHCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCEEEECCCEEEECCCC
TGIGSGLDKTMSDYVTRVDYSPNRTYTLSMRSRLDEQSLNVQRFEAEGKVNFDRWSAALT
CCCCCCHHHHHHHHHEEEECCCCCEEEEEHHHCCCHHCCCEEEEECCCCCCHHHHEEEEE
YGNYAAQPAIGNLDRREGLLGNGSVKVAQNWVVSGGARWDLVANKMNQYLVGAGYVDDCF
ECCEECCCCCCCCHHHCCCCCCCCEEEEEEEEECCCCEEEHHHHHHCCEEEECCCHHHHH
TLAASYVTSTSYAIAGATPQTNQTVMLTLGLRTIGNVSLPVPVN
HHHHHHHHCCCEEEECCCCCCCCEEEEEEECHHCCCEEECCCCC
>Mature Secondary Structure 
TKPARVTDRCFARSRTASTDSRRVRALRRSPAALAVCVVLAAGAVLPATAYAQSFTYNP
CCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCC
LPPKPPPPRVANDNQMLVQATTVEYDANNSRVAAVGNVQLFYNGSSVEADRVIYDQKTKR
CCCCCCCCCCCCCCEEEEEEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCEEECCHHHE
LHAEGNIRMTDADGKITYADSLDLSDDYRDGFVDSLRVETEDQTRMAATRADRSKGNTTV
EECCCCEEEECCCCEEEEECCCCCCCCHHCCCCHHEEECCCCHHHHHHHHHHCCCCCCEE
FENGVYTACAPCRDDPKKPPLWQVKGARIIHDQTEKMLYFENAQLEMFGVPLAYIPYFST
EECCCEEEECCCCCCCCCCCEEEECCCEEEECCCCEEEEEECCEEEEECCCCEECCCCCC
PDPTVKRKSGFLMPGFSQNNAYGIGVDIPYYWAIDGSYDATFTPRLTSRQGVLVQTEFRQ
CCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCEECCCCCCCCCEEEEHHHHH
RLDNGSYQVRFYGIDQLDRNAFAGQPGDRQLRGGIDSRGQLALSDKWTFGWDGVLLSDYT
HHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCEEEEECCEECCCCCEEEECEE
FLSDYRLVQYRDPFNAFMTLPTEAISQLYLTGVGSRSVFDIRAMHYLSFSGNQQTVPIVY
EECCEEEEEECCCHHHEEECCHHHHHHEEEECCCCCCEEEEEEEEEEEECCCCEEEEEEE
PVLDYSNVLNSPVLGGEFSTKTNFVNLTREQAVFDPVTTTANTNGLCTTASADPLARLPS
EECCHHHHHCCCCCCCCCCCCCCEEEEEHHHHEECCCCCCCCCCCEEEECCCCHHHHCCH
QCLLRAAPGTYTRLTAQAEWRKSFTDPMGEIWTPFAILRADAINANVTAQPGVGNFLPTG
HHHEECCCCCEEEEEECHHHHHHHCCCHHHHHCCCEEEEECCCCCCEEECCCCCCCCCCC
DTQALRVTPTVGLDYRYPFINVQSWGTTTVEPIAQLIVRPNETSAGRLPNEDAQSLVFDA
CCCEEEEECEECCCEECCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEC
SNLFSVDKFSGYDRVAGGGRANIGAQATTQFDRGGTVNLLFGQSTQLFGLNSYAVQDANN
CCCEEEHHCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCEEEECCCEEEECCCC
TGIGSGLDKTMSDYVTRVDYSPNRTYTLSMRSRLDEQSLNVQRFEAEGKVNFDRWSAALT
CCCCCCHHHHHHHHHEEEECCCCCEEEEEHHHCCCHHCCCEEEEECCCCCCHHHHEEEEE
YGNYAAQPAIGNLDRREGLLGNGSVKVAQNWVVSGGARWDLVANKMNQYLVGAGYVDDCF
ECCEECCCCCCCCHHHCCCCCCCCEEEEEEEEECCCCEEEHHHHHHCCEEEECCCHHHHH
TLAASYVTSTSYAIAGATPQTNQTVMLTLGLRTIGNVSLPVPVN
HHHHHHHHCCCEEEECCCCCCCCEEEEEEECHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA