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The map label for this gene is bcr [H]

Identifier: 146342487

GI number: 146342487

Start: 5836032

End: 5837315

Strand: Direct

Name: bcr [H]

Synonym: BRADO5648

Alternate gene names: 146342487

Gene position: 5836032-5837315 (Clockwise)

Preceding gene: 146342486

Following gene: 146342507

Centisome position: 78.27

GC content: 67.13

Gene sequence:

>1284_bases
ATGCATGGAATGATCAGCAGACCGCCGGGAGCCGTCGCACAGCGCGCGGCTCCGTCGCGCACGCTGCTGGTGCTGCTCGT
CGTCATGACCGGACTGGCGCCGATCTCGCTGTACCTGCTGGTGCCGGCGCTGCCGTTGCTCGCGACCACCTTCGGGCGCG
ACGCCTCGGTGGTGCAGATGACGGTGTCGTTCTACATGATCGGCATTGCCATCTCGCAGCTGTTGCTGGGCCCGCTGTCC
GATCGCTTCGGCCGCCGTCCGGTGATGCTCGCCGGCCTTGCGCTCTCCATCATCGCGAGCTTTGGCTGCATCTTGGCCGA
CACCCTGCCGCAGCTGATCGCGGCGCGCTTCTTTCAGGCGCTCGGCGGCGCCAGCGGCATGGTGATCAGCAGGGCGATCA
TCCGCGACCTCTATCCGCGCGAGCGCATTGGCGCGATGATCAGCCTCGTCGTCGCCGTCATGATGATCGCGCAGATGCTG
TCGCCGCTGACCGGCGGCCTGATCGAGATCGCGTTCGGCTGGCATGCCATCTTCTACGTGGTGGCAGCGGTGTCGATTGC
CGTCGGCATCGCTGTAACGCTGGCGCTGCCGGAAACCCGCCCAGCCCGCGTCGAGGGCGCCAGCTTCCGCCGCGACGTGG
TCATACTCGTCAAGAGCCGCGCCTTTCTGGGCTACATGCTGTGCCAGGTGCTGGCGTCGCAGATCATCTTCGCCTTTGCC
GGCGGCGGCCCCTACATCGTCGAGGTGCAGATGGCCCGCTCGTCGGCAGAGTATGGCGCCTGGTTCGCGACGACAGGCTT
TGCCTATTTCGTCGGGAATCTGTTCTGCGTCCGCTACGCGCCGCGGCACGCGCTCGACCGGCTGATCTGGTTCGGCCTGA
CCTTGCAGCTGACCAGCGCCGCGCTGAATTTCACCTGGGGCATCTTCGGTCTCAACCAGATGCCGTCGGTGCTGTTCGGC
ACGCAGATGCTGCTGATGTTCGCCAACGCCTTCGTGATGGCAAACTCCGCCGCCGGCGCCATCAGCGTGCGGCCGGGCGC
CGCCGGCACCGCCTCGGGCGCGATGGGCTTTCTGCAGATGGGGCTGGGCGCGCTGACCTCGCAGCTCGGGGCGTATCTCG
GCGGTCACTATGCGACCACGCTGCCCCTGACGTCGGCGATGCTGACGCTGTCGCTGGCCTGCGCCGGCTGCATGATCTTC
CTCGTTCCCCGCACGCCGGTCACGGTGACGGTGACCAAGGAGCTGATCGAAGCGGCGGAGGAGGACGAGGGCGGGGTGAT
GTGA

Upstream 100 bases:

>100_bases
GCCATGGTCCCGCTGCCGGTATGGGCGCATGCAGAAACAGGCGTTTCCGTGACGCGGAAGGCCGCTACAGTCGCGGCGTC
CACCTGATTTGAGCGGCACG

Downstream 100 bases:

>100_bases
GGGATCGCGCGCTTTCGCATGTCTCTCGGTGTCATACCCGCGAAGGCGGGTATCCAGTACGCCGCGGCGGCGCGGCTCCA
TCACAACCGCCTCTGGAATA

Product: Bcr/CflA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MHGMISRPPGAVAQRAAPSRTLLVLLVVMTGLAPISLYLLVPALPLLATTFGRDASVVQMTVSFYMIGIAISQLLLGPLS
DRFGRRPVMLAGLALSIIASFGCILADTLPQLIAARFFQALGGASGMVISRAIIRDLYPRERIGAMISLVVAVMMIAQML
SPLTGGLIEIAFGWHAIFYVVAAVSIAVGIAVTLALPETRPARVEGASFRRDVVILVKSRAFLGYMLCQVLASQIIFAFA
GGGPYIVEVQMARSSAEYGAWFATTGFAYFVGNLFCVRYAPRHALDRLIWFGLTLQLTSAALNFTWGIFGLNQMPSVLFG
TQMLLMFANAFVMANSAAGAISVRPGAAGTASGAMGFLQMGLGALTSQLGAYLGGHYATTLPLTSAMLTLSLACAGCMIF
LVPRTPVTVTVTKELIEAAEEDEGGVM

Sequences:

>Translated_427_residues
MHGMISRPPGAVAQRAAPSRTLLVLLVVMTGLAPISLYLLVPALPLLATTFGRDASVVQMTVSFYMIGIAISQLLLGPLS
DRFGRRPVMLAGLALSIIASFGCILADTLPQLIAARFFQALGGASGMVISRAIIRDLYPRERIGAMISLVVAVMMIAQML
SPLTGGLIEIAFGWHAIFYVVAAVSIAVGIAVTLALPETRPARVEGASFRRDVVILVKSRAFLGYMLCQVLASQIIFAFA
GGGPYIVEVQMARSSAEYGAWFATTGFAYFVGNLFCVRYAPRHALDRLIWFGLTLQLTSAALNFTWGIFGLNQMPSVLFG
TQMLLMFANAFVMANSAAGAISVRPGAAGTASGAMGFLQMGLGALTSQLGAYLGGHYATTLPLTSAMLTLSLACAGCMIF
LVPRTPVTVTVTKELIEAAEEDEGGVM
>Mature_427_residues
MHGMISRPPGAVAQRAAPSRTLLVLLVVMTGLAPISLYLLVPALPLLATTFGRDASVVQMTVSFYMIGIAISQLLLGPLS
DRFGRRPVMLAGLALSIIASFGCILADTLPQLIAARFFQALGGASGMVISRAIIRDLYPRERIGAMISLVVAVMMIAQML
SPLTGGLIEIAFGWHAIFYVVAAVSIAVGIAVTLALPETRPARVEGASFRRDVVILVKSRAFLGYMLCQVLASQIIFAFA
GGGPYIVEVQMARSSAEYGAWFATTGFAYFVGNLFCVRYAPRHALDRLIWFGLTLQLTSAALNFTWGIFGLNQMPSVLFG
TQMLLMFANAFVMANSAAGAISVRPGAAGTASGAMGFLQMGLGALTSQLGAYLGGHYATTLPLTSAMLTLSLACAGCMIF
LVPRTPVTVTVTKELIEAAEEDEGGVM

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=366, Percent_Identity=31.6939890710383, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI48994889, Length=380, Percent_Identity=27.8947368421053, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1790146, Length=347, Percent_Identity=32.2766570605187, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI87082312, Length=328, Percent_Identity=28.0487804878049, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787065, Length=313, Percent_Identity=28.4345047923323, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1790195, Length=153, Percent_Identity=30.718954248366, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790794, Length=274, Percent_Identity=24.0875912408759, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87081983, Length=171, Percent_Identity=29.2397660818713, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=159, Percent_Identity=30.188679245283, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322071, Length=213, Percent_Identity=26.2910798122066, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325414, Length=140, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321577, Length=148, Percent_Identity=27.7027027027027, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322070, Length=197, Percent_Identity=28.4263959390863, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45121; Mature: 45121

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGMISRPPGAVAQRAAPSRTLLVLLVVMTGLAPISLYLLVPALPLLATTFGRDASVVQM
CCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TVSFYMIGIAISQLLLGPLSDRFGRRPVMLAGLALSIIASFGCILADTLPQLIAARFFQA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGASGMVISRAIIRDLYPRERIGAMISLVVAVMMIAQMLSPLTGGLIEIAFGWHAIFYV
HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VAAVSIAVGIAVTLALPETRPARVEGASFRRDVVILVKSRAFLGYMLCQVLASQIIFAFA
HHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHEE
GGGPYIVEVQMARSSAEYGAWFATTGFAYFVGNLFCVRYAPRHALDRLIWFGLTLQLTSA
CCCCEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ALNFTWGIFGLNQMPSVLFGTQMLLMFANAFVMANSAAGAISVRPGAAGTASGAMGFLQM
HHHHHHHHHCHHHCHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCHHHHHHHHH
GLGALTSQLGAYLGGHYATTLPLTSAMLTLSLACAGCMIFLVPRTPVTVTVTKELIEAAE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEHHHHHHHHC
EDEGGVM
CCCCCCC
>Mature Secondary Structure
MHGMISRPPGAVAQRAAPSRTLLVLLVVMTGLAPISLYLLVPALPLLATTFGRDASVVQM
CCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TVSFYMIGIAISQLLLGPLSDRFGRRPVMLAGLALSIIASFGCILADTLPQLIAARFFQA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGASGMVISRAIIRDLYPRERIGAMISLVVAVMMIAQMLSPLTGGLIEIAFGWHAIFYV
HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VAAVSIAVGIAVTLALPETRPARVEGASFRRDVVILVKSRAFLGYMLCQVLASQIIFAFA
HHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHEE
GGGPYIVEVQMARSSAEYGAWFATTGFAYFVGNLFCVRYAPRHALDRLIWFGLTLQLTSA
CCCCEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ALNFTWGIFGLNQMPSVLFGTQMLLMFANAFVMANSAAGAISVRPGAAGTASGAMGFLQM
HHHHHHHHHCHHHCHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCHHHHHHHHH
GLGALTSQLGAYLGGHYATTLPLTSAMLTLSLACAGCMIFLVPRTPVTVTVTKELIEAAE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEHHHHHHHHC
EDEGGVM
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]