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The map label for this gene is amiD [H]

Identifier: 146342507

GI number: 146342507

Start: 5864268

End: 5865119

Strand: Direct

Name: amiD [H]

Synonym: BRADO5669

Alternate gene names: 146342507

Gene position: 5864268-5865119 (Clockwise)

Preceding gene: 146342487

Following gene: 146342513

Centisome position: 78.65

GC content: 65.02

Gene sequence:

>852_bases
ATGCCAAATTCCTCCGACAAGCCGGATTCGAAGCGTCTTCGCGCGCTGCCGATGCCCAAGGGCGTGTCTGACCTGCCCAT
GTTCACGCCGGACTCGTCGATCGCGTCCGATGTGATCCCGTCGGCCAATTTCGGCGAACGCAAGGGCGGCCGTCAGCCGG
ACATGATCATCCTCCACTATACCGGGATGCCGGATGCCGAGGGTGCGATCAACAAACTTTGCACCGCCGGAACCGAAGTG
TCTGCGCACTACATCGTTCTCGAGGACGGCCGCATCGTCCAATGCGTGCCCGAGGCTCTGCGCGCCTGGCATGCGGGGCT
TTCGTCCTGGGGCGGCGAGGAGGACATCAATTCGTGCTCGATCGGGATCGAGATCGTCAACCGCGGCCACGACTGGGGCT
ACCCGGATTTTCCGCCACGCCAGATCGCAGCCGTCATCGCGCTGTGCCGCGGCATCATGCTCCGCCGCAACGTGCCGGCG
CACCGCGTGCTCGGGCATTCCGATGTGGCGCCGTCGCGCAAGAAGGACCCGGGCGAGAAGTTTCCCTGGCACTCCCTCGC
CAATTCCGGCGTGGGCCACTGGGTCCAGCCGGCGGCGATCACCAAGGGCGAGACCCTGAAGCTCGGGAGCATCGGCGATG
ACGTCAAAGGCTTGCAGAAGGCGCTCGCGAAATATGGCTATGGCGTGCCGATCACCGGCAAGTTCGATGGTCTCACGATG
GAGGTCGTCACGGCGTTCCAGCGCCACTTTCGTCCCGAGCGGGTCGACGGCATCGCCGACCGCTCGACTTTGTGGACGCT
GCATGCGCTGCTGACCAGTCTGCCGGCCGAAGAGACCGTGCCGCGGGCTTGA

Upstream 100 bases:

>100_bases
GTTCGCATGCGATTTCGATTGCGGCTGGGACATGTAAAGGAGTAGTGCCACCCCATATTCGATTTGAGCCATTGCCCTCG
CCGCCCGCTTCGAGTCGTTC

Downstream 100 bases:

>100_bases
CGCGTCAAATTGTGGCATCGAGTTGCCCGATCCGGCGCGCATAGAGTCGCTTGAGCGGAGCAAGCTGCGTCGCATCGCTC
GCCGCCTGATAGGCCACACG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MPNSSDKPDSKRLRALPMPKGVSDLPMFTPDSSIASDVIPSANFGERKGGRQPDMIILHYTGMPDAEGAINKLCTAGTEV
SAHYIVLEDGRIVQCVPEALRAWHAGLSSWGGEEDINSCSIGIEIVNRGHDWGYPDFPPRQIAAVIALCRGIMLRRNVPA
HRVLGHSDVAPSRKKDPGEKFPWHSLANSGVGHWVQPAAITKGETLKLGSIGDDVKGLQKALAKYGYGVPITGKFDGLTM
EVVTAFQRHFRPERVDGIADRSTLWTLHALLTSLPAEETVPRA

Sequences:

>Translated_283_residues
MPNSSDKPDSKRLRALPMPKGVSDLPMFTPDSSIASDVIPSANFGERKGGRQPDMIILHYTGMPDAEGAINKLCTAGTEV
SAHYIVLEDGRIVQCVPEALRAWHAGLSSWGGEEDINSCSIGIEIVNRGHDWGYPDFPPRQIAAVIALCRGIMLRRNVPA
HRVLGHSDVAPSRKKDPGEKFPWHSLANSGVGHWVQPAAITKGETLKLGSIGDDVKGLQKALAKYGYGVPITGKFDGLTM
EVVTAFQRHFRPERVDGIADRSTLWTLHALLTSLPAEETVPRA
>Mature_282_residues
PNSSDKPDSKRLRALPMPKGVSDLPMFTPDSSIASDVIPSANFGERKGGRQPDMIILHYTGMPDAEGAINKLCTAGTEVS
AHYIVLEDGRIVQCVPEALRAWHAGLSSWGGEEDINSCSIGIEIVNRGHDWGYPDFPPRQIAAVIALCRGIMLRRNVPAH
RVLGHSDVAPSRKKDPGEKFPWHSLANSGVGHWVQPAAITKGETLKLGSIGDDVKGLQKALAKYGYGVPITGKFDGLTME
VVTAFQRHFRPERVDGIADRSTLWTLHALLTSLPAEETVPRA

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=234, Percent_Identity=37.6068376068376, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1786300, Length=163, Percent_Identity=36.1963190184049, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 30713; Mature: 30582

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNSSDKPDSKRLRALPMPKGVSDLPMFTPDSSIASDVIPSANFGERKGGRQPDMIILHY
CCCCCCCCHHHHHEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
TGMPDAEGAINKLCTAGTEVSAHYIVLEDGRIVQCVPEALRAWHAGLSSWGGEEDINSCS
CCCCCHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHE
IGIEIVNRGHDWGYPDFPPRQIAAVIALCRGIMLRRNVPAHRVLGHSDVAPSRKKDPGEK
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCC
FPWHSLANSGVGHWVQPAAITKGETLKLGSIGDDVKGLQKALAKYGYGVPITGKFDGLTM
CCHHHHHCCCCCCCCCCCEECCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHH
EVVTAFQRHFRPERVDGIADRSTLWTLHALLTSLPAEETVPRA
HHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PNSSDKPDSKRLRALPMPKGVSDLPMFTPDSSIASDVIPSANFGERKGGRQPDMIILHY
CCCCCCCHHHHHEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
TGMPDAEGAINKLCTAGTEVSAHYIVLEDGRIVQCVPEALRAWHAGLSSWGGEEDINSCS
CCCCCHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHE
IGIEIVNRGHDWGYPDFPPRQIAAVIALCRGIMLRRNVPAHRVLGHSDVAPSRKKDPGEK
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCC
FPWHSLANSGVGHWVQPAAITKGETLKLGSIGDDVKGLQKALAKYGYGVPITGKFDGLTM
CCHHHHHCCCCCCCCCCCEECCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHH
EVVTAFQRHFRPERVDGIADRSTLWTLHALLTSLPAEETVPRA
HHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]