| Definition | Bradyrhizobium sp. ORS278 chromosome, complete genome. |
|---|---|
| Accession | NC_009445 |
| Length | 7,456,587 |
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The map label for this gene is braF [H]
Identifier: 146342435
GI number: 146342435
Start: 5778070
End: 5778813
Strand: Reverse
Name: braF [H]
Synonym: BRADO5594
Alternate gene names: 146342435
Gene position: 5778813-5778070 (Counterclockwise)
Preceding gene: 146342436
Following gene: 146342434
Centisome position: 77.5
GC content: 65.32
Gene sequence:
>744_bases ATGACCGCAGTGCTCGATGTCCGCGACGTCAAGAAGAGCTTCGGCGGCATCACCGCCGTCGACGGCGTCAGCTTCGACGT GCAGGAGGGCGAGATCCTCGGCCTGATCGGGCCCAACGGCTGCGGCAAGTCGACCTTGTTCAACTGCATCCTCGGCCAGC TCGTACCGAGTGCGGGTGAAGTGCGGGTCGATGGCAAGCTCGTCACCGGACAGCGGCCGGCGGAGCTCAACCGGCTCGGC GTCAGCCGCACCTTCCAGCTGCTGCAGGTATTTCCGAAACTCTCGGTGCGCGACAATCTGATCCTCGCCGGACAGGAGCA TCGCGGCAGCATGCTGTCGCGCCTGTTCGGTCCGTCCGATGCGGGCCTCACCGAAACCGCCGATCAGATGATCGGCTTCT TCAAGCTGGAGCATCTCGCCAACGAGCCGGCCGGCGGCCTGTCCTACGGCCAGCAGAAGCTGCTGGATGCCGCCATGGCG TTCATGGGCGGCCCGCGGCTGGTGCTGCTCGACGAGCCCGCAGGCGGCGTCAATCCGACCATGCTGGGCGACCTCAAGGA GCGGCTGATCGCGATCAACCGCGAGCGTCGCGCCACCTTCGTCGTGATCGAGCACAACATGGAGTTCGTGATGTCGCTGT GCTCGCGCGTCATGGTCATGGCGGAAGGCAAGGTCTTGGCGATCGGCAAGCCGGACGAGATCAGGGCCAATCCGGCCGTG GTCGAAGCCTATCTCGGACACTGA
Upstream 100 bases:
>100_bases CCGGCATTCTCGGCATCCTCGATCGTTATCTCAGCTCACGCCGGACACAGGCGGCGTCCGCGTTGCGCGCAGCGGCGCAA TCCAAGCTGGAGACCGCGCA
Downstream 100 bases:
>100_bases CGGAGGCGGCCGTGAGCGAACCCATTCTCGATGTCCAGAACCTGGTCGGCGGCTACGGCAAGATGACGATCCTGAACGGC ACGACCTTTTCGGTGCCGAA
Product: putative branched-chain amino acid ABC transporter ATP-binding protein
Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]
Alternate protein names: NA
Number of amino acids: Translated: 247; Mature: 246
Protein sequence:
>247_residues MTAVLDVRDVKKSFGGITAVDGVSFDVQEGEILGLIGPNGCGKSTLFNCILGQLVPSAGEVRVDGKLVTGQRPAELNRLG VSRTFQLLQVFPKLSVRDNLILAGQEHRGSMLSRLFGPSDAGLTETADQMIGFFKLEHLANEPAGGLSYGQQKLLDAAMA FMGGPRLVLLDEPAGGVNPTMLGDLKERLIAINRERRATFVVIEHNMEFVMSLCSRVMVMAEGKVLAIGKPDEIRANPAV VEAYLGH
Sequences:
>Translated_247_residues MTAVLDVRDVKKSFGGITAVDGVSFDVQEGEILGLIGPNGCGKSTLFNCILGQLVPSAGEVRVDGKLVTGQRPAELNRLG VSRTFQLLQVFPKLSVRDNLILAGQEHRGSMLSRLFGPSDAGLTETADQMIGFFKLEHLANEPAGGLSYGQQKLLDAAMA FMGGPRLVLLDEPAGGVNPTMLGDLKERLIAINRERRATFVVIEHNMEFVMSLCSRVMVMAEGKVLAIGKPDEIRANPAV VEAYLGH >Mature_246_residues TAVLDVRDVKKSFGGITAVDGVSFDVQEGEILGLIGPNGCGKSTLFNCILGQLVPSAGEVRVDGKLVTGQRPAELNRLGV SRTFQLLQVFPKLSVRDNLILAGQEHRGSMLSRLFGPSDAGLTETADQMIGFFKLEHLANEPAGGLSYGQQKLLDAAMAF MGGPRLVLLDEPAGGVNPTMLGDLKERLIAINRERRATFVVIEHNMEFVMSLCSRVMVMAEGKVLAIGKPDEIRANPAVV EAYLGH
Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]
COG id: COG0411
COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component
Gene ontology:
Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 ABC transporter domain [H]
Homologues:
Organism=Homo sapiens, GI150417984, Length=234, Percent_Identity=32.0512820512821, Blast_Score=109, Evalue=3e-24, Organism=Homo sapiens, GI27881501, Length=221, Percent_Identity=29.4117647058824, Blast_Score=103, Evalue=2e-22, Organism=Homo sapiens, GI30795238, Length=221, Percent_Identity=29.4117647058824, Blast_Score=103, Evalue=2e-22, Organism=Homo sapiens, GI105990541, Length=237, Percent_Identity=31.2236286919831, Blast_Score=101, Evalue=6e-22, Organism=Homo sapiens, GI27436953, Length=217, Percent_Identity=29.9539170506912, Blast_Score=98, Evalue=8e-21, Organism=Homo sapiens, GI31657092, Length=225, Percent_Identity=30.6666666666667, Blast_Score=96, Evalue=2e-20, Organism=Homo sapiens, GI27262624, Length=230, Percent_Identity=26.9565217391304, Blast_Score=93, Evalue=2e-19, Organism=Homo sapiens, GI27262626, Length=230, Percent_Identity=26.9565217391304, Blast_Score=93, Evalue=2e-19, Organism=Homo sapiens, GI47078218, Length=240, Percent_Identity=29.1666666666667, Blast_Score=92, Evalue=3e-19, Organism=Homo sapiens, GI45446740, Length=240, Percent_Identity=29.1666666666667, Blast_Score=92, Evalue=4e-19, Organism=Homo sapiens, GI116734710, Length=224, Percent_Identity=27.6785714285714, Blast_Score=90, Evalue=2e-18, Organism=Homo sapiens, GI153792144, Length=219, Percent_Identity=30.1369863013699, Blast_Score=90, Evalue=2e-18, Organism=Homo sapiens, GI6005701, Length=219, Percent_Identity=30.5936073059361, Blast_Score=86, Evalue=2e-17, Organism=Homo sapiens, GI21536376, Length=216, Percent_Identity=28.7037037037037, Blast_Score=86, Evalue=4e-17, Organism=Homo sapiens, GI27477115, Length=219, Percent_Identity=30.1369863013699, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI11967971, Length=239, Percent_Identity=25.1046025104602, Blast_Score=72, Evalue=5e-13, Organism=Homo sapiens, GI11967969, Length=221, Percent_Identity=24.4343891402715, Blast_Score=65, Evalue=5e-11, Organism=Homo sapiens, GI4557481, Length=224, Percent_Identity=25, Blast_Score=65, Evalue=6e-11, Organism=Escherichia coli, GI1789864, Length=250, Percent_Identity=36.4, Blast_Score=160, Evalue=6e-41, Organism=Escherichia coli, GI87081791, Length=240, Percent_Identity=30, Blast_Score=124, Evalue=7e-30, Organism=Escherichia coli, GI1789593, Length=244, Percent_Identity=30.7377049180328, Blast_Score=117, Evalue=6e-28, Organism=Escherichia coli, GI1788761, Length=236, Percent_Identity=28.3898305084746, Blast_Score=113, Evalue=1e-26, Organism=Escherichia coli, GI48994943, Length=247, Percent_Identity=31.5789473684211, Blast_Score=112, Evalue=2e-26, Organism=Escherichia coli, GI87081782, Length=242, Percent_Identity=31.404958677686, Blast_Score=108, Evalue=4e-25, Organism=Escherichia coli, GI87082268, Length=244, Percent_Identity=30.7377049180328, Blast_Score=108, Evalue=4e-25, Organism=Escherichia coli, GI87081709, Length=235, Percent_Identity=25.1063829787234, Blast_Score=107, Evalue=9e-25, Organism=Escherichia coli, GI1787370, Length=229, Percent_Identity=29.6943231441048, Blast_Score=105, Evalue=2e-24, Organism=Escherichia coli, GI1790467, Length=237, Percent_Identity=26.5822784810127, Blast_Score=105, Evalue=3e-24, Organism=Escherichia coli, GI1788210, Length=250, Percent_Identity=26.4, Blast_Score=102, Evalue=3e-23, Organism=Escherichia coli, GI1789672, Length=238, Percent_Identity=28.1512605042017, Blast_Score=96, Evalue=1e-21, Organism=Escherichia coli, GI87082267, Length=247, Percent_Identity=27.1255060728745, Blast_Score=96, Evalue=2e-21, Organism=Escherichia coli, GI1787712, Length=238, Percent_Identity=27.3109243697479, Blast_Score=96, Evalue=3e-21, Organism=Escherichia coli, GI1790190, Length=231, Percent_Identity=26.8398268398268, Blast_Score=95, Evalue=4e-21, Organism=Escherichia coli, GI1788472, Length=227, Percent_Identity=29.5154185022026, Blast_Score=94, Evalue=6e-21, Organism=Escherichia coli, GI48995001, Length=223, Percent_Identity=25.5605381165919, Blast_Score=94, Evalue=7e-21, Organism=Escherichia coli, GI1788450, Length=237, Percent_Identity=28.2700421940928, Blast_Score=94, Evalue=8e-21, Organism=Escherichia coli, GI1788225, Length=243, Percent_Identity=26.3374485596708, Blast_Score=94, Evalue=9e-21, Organism=Escherichia coli, GI1789032, Length=230, Percent_Identity=28.695652173913, Blast_Score=93, Evalue=2e-20, Organism=Escherichia coli, GI1786803, Length=224, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=3e-20, Organism=Escherichia coli, GI1787029, Length=250, Percent_Identity=26, Blast_Score=92, Evalue=3e-20, Organism=Escherichia coli, GI1789586, Length=237, Percent_Identity=29.535864978903, Blast_Score=92, Evalue=4e-20, Organism=Escherichia coli, GI1790739, Length=238, Percent_Identity=30.2521008403361, Blast_Score=91, Evalue=6e-20, Organism=Escherichia coli, GI1787500, Length=245, Percent_Identity=27.7551020408163, Blast_Score=89, Evalue=2e-19, Organism=Escherichia coli, GI1786872, Length=237, Percent_Identity=29.957805907173, Blast_Score=89, Evalue=2e-19, Organism=Escherichia coli, GI1787792, Length=254, Percent_Identity=25.9842519685039, Blast_Score=89, Evalue=3e-19, Organism=Escherichia coli, GI1788165, Length=244, Percent_Identity=27.8688524590164, Blast_Score=88, Evalue=5e-19, Organism=Escherichia coli, GI1790162, Length=248, Percent_Identity=27.8225806451613, Blast_Score=88, Evalue=6e-19, Organism=Escherichia coli, GI1788897, Length=223, Percent_Identity=30.9417040358744, Blast_Score=86, Evalue=3e-18, Organism=Escherichia coli, GI1790544, Length=244, Percent_Identity=25.8196721311475, Blast_Score=85, Evalue=4e-18, Organism=Escherichia coli, GI87081834, Length=214, Percent_Identity=28.0373831775701, Blast_Score=84, Evalue=8e-18, Organism=Escherichia coli, GI1786398, Length=224, Percent_Identity=28.125, Blast_Score=83, Evalue=2e-17, Organism=Escherichia coli, GI1787089, Length=225, Percent_Identity=27.5555555555556, Blast_Score=83, Evalue=2e-17, Organism=Escherichia coli, GI1786563, Length=222, Percent_Identity=26.5765765765766, Blast_Score=82, Evalue=3e-17, Organism=Escherichia coli, GI1786253, Length=223, Percent_Identity=27.8026905829596, Blast_Score=81, Evalue=6e-17, Organism=Escherichia coli, GI48994997, Length=238, Percent_Identity=25.2100840336134, Blast_Score=81, Evalue=7e-17, Organism=Escherichia coli, GI1789991, Length=235, Percent_Identity=26.3829787234043, Blast_Score=80, Evalue=1e-16, Organism=Escherichia coli, GI48994883, Length=252, Percent_Identity=25.3968253968254, Blast_Score=80, Evalue=1e-16, Organism=Escherichia coli, GI1788644, Length=254, Percent_Identity=28.3464566929134, Blast_Score=79, Evalue=3e-16, Organism=Escherichia coli, GI1789962, Length=236, Percent_Identity=26.6949152542373, Blast_Score=76, Evalue=2e-15, Organism=Escherichia coli, GI1787105, Length=254, Percent_Identity=27.1653543307087, Blast_Score=76, Evalue=2e-15, Organism=Escherichia coli, GI1786703, Length=233, Percent_Identity=27.8969957081545, Blast_Score=75, Evalue=5e-15, Organism=Escherichia coli, GI1786345, Length=228, Percent_Identity=27.6315789473684, Blast_Score=74, Evalue=6e-15, Organism=Escherichia coli, GI1787112, Length=241, Percent_Identity=29.045643153527, Blast_Score=74, Evalue=6e-15, Organism=Escherichia coli, GI1790525, Length=250, Percent_Identity=24.4, Blast_Score=74, Evalue=7e-15, Organism=Escherichia coli, GI145693107, Length=226, Percent_Identity=26.5486725663717, Blast_Score=72, Evalue=3e-14, Organism=Escherichia coli, GI1787547, Length=258, Percent_Identity=23.6434108527132, Blast_Score=72, Evalue=4e-14, Organism=Escherichia coli, GI1788540, Length=228, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=6e-13, Organism=Escherichia coli, GI1788506, Length=257, Percent_Identity=24.9027237354086, Blast_Score=68, Evalue=7e-13, Organism=Escherichia coli, GI1787164, Length=218, Percent_Identity=25.2293577981651, Blast_Score=68, Evalue=7e-13, Organism=Escherichia coli, GI2367384, Length=224, Percent_Identity=27.6785714285714, Blast_Score=68, Evalue=7e-13, Organism=Escherichia coli, GI1789963, Length=255, Percent_Identity=25.8823529411765, Blast_Score=65, Evalue=4e-12, Organism=Escherichia coli, GI1789873, Length=221, Percent_Identity=28.0542986425339, Blast_Score=64, Evalue=9e-12, Organism=Escherichia coli, GI1788053, Length=226, Percent_Identity=26.5486725663717, Blast_Score=61, Evalue=6e-11, Organism=Escherichia coli, GI1787182, Length=232, Percent_Identity=28.0172413793103, Blast_Score=60, Evalue=9e-11, Organism=Caenorhabditis elegans, GI71983523, Length=241, Percent_Identity=27.3858921161826, Blast_Score=104, Evalue=5e-23, Organism=Caenorhabditis elegans, GI71983516, Length=241, Percent_Identity=27.3858921161826, Blast_Score=104, Evalue=5e-23, Organism=Caenorhabditis elegans, GI71983510, Length=241, Percent_Identity=27.3858921161826, Blast_Score=104, Evalue=5e-23, Organism=Caenorhabditis elegans, GI115533608, Length=239, Percent_Identity=29.2887029288703, Blast_Score=97, Evalue=1e-20, Organism=Caenorhabditis elegans, GI17565586, Length=214, Percent_Identity=29.9065420560748, Blast_Score=89, Evalue=3e-18, Organism=Caenorhabditis elegans, GI17510237, Length=237, Percent_Identity=24.8945147679325, Blast_Score=86, Evalue=2e-17, Organism=Caenorhabditis elegans, GI115534520, Length=245, Percent_Identity=27.3469387755102, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI193208177, Length=224, Percent_Identity=25.8928571428571, Blast_Score=66, Evalue=2e-11, Organism=Drosophila melanogaster, GI28571209, Length=228, Percent_Identity=28.5087719298246, Blast_Score=94, Evalue=9e-20, Organism=Drosophila melanogaster, GI116007328, Length=220, Percent_Identity=31.3636363636364, Blast_Score=91, Evalue=6e-19, Organism=Drosophila melanogaster, GI28574150, Length=220, Percent_Identity=31.3636363636364, Blast_Score=91, Evalue=6e-19, Organism=Drosophila melanogaster, GI24666092, Length=227, Percent_Identity=28.1938325991189, Blast_Score=86, Evalue=2e-17, Organism=Drosophila melanogaster, GI24643674, Length=226, Percent_Identity=30.0884955752212, Blast_Score=85, Evalue=4e-17, Organism=Drosophila melanogaster, GI116007184, Length=225, Percent_Identity=29.3333333333333, Blast_Score=81, Evalue=5e-16, Organism=Drosophila melanogaster, GI221500365, Length=225, Percent_Identity=29.3333333333333, Blast_Score=81, Evalue=5e-16, Organism=Drosophila melanogaster, GI28573571, Length=228, Percent_Identity=26.3157894736842, Blast_Score=81, Evalue=6e-16, Organism=Drosophila melanogaster, GI221512771, Length=222, Percent_Identity=27.9279279279279, Blast_Score=81, Evalue=6e-16, Organism=Drosophila melanogaster, GI24650855, Length=210, Percent_Identity=27.6190476190476, Blast_Score=80, Evalue=9e-16, Organism=Drosophila melanogaster, GI24650853, Length=210, Percent_Identity=27.6190476190476, Blast_Score=80, Evalue=9e-16, Organism=Drosophila melanogaster, GI85725262, Length=243, Percent_Identity=26.7489711934156, Blast_Score=80, Evalue=2e-15, Organism=Drosophila melanogaster, GI281362751, Length=210, Percent_Identity=27.6190476190476, Blast_Score=79, Evalue=2e-15, Organism=Drosophila melanogaster, GI24648314, Length=225, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=2e-15, Organism=Drosophila melanogaster, GI28573573, Length=223, Percent_Identity=26.457399103139, Blast_Score=78, Evalue=4e-15, Organism=Drosophila melanogaster, GI24582014, Length=222, Percent_Identity=28.3783783783784, Blast_Score=78, Evalue=7e-15, Organism=Drosophila melanogaster, GI24582012, Length=222, Percent_Identity=28.3783783783784, Blast_Score=78, Evalue=7e-15,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003439 - InterPro: IPR017871 - InterPro: IPR003593 [H]
Pfam domain/function: PF00005 ABC_tran [H]
EC number: NA
Molecular weight: Translated: 26517; Mature: 26386
Theoretical pI: Translated: 6.14; Mature: 6.14
Prosite motif: PS50893 ABC_TRANSPORTER_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 4.0 %Met (Translated Protein) 5.3 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 3.7 %Met (Mature Protein) 4.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTAVLDVRDVKKSFGGITAVDGVSFDVQEGEILGLIGPNGCGKSTLFNCILGQLVPSAGE CCCEEHHHHHHHHHCCEEEECCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCC VRVDGKLVTGQRPAELNRLGVSRTFQLLQVFPKLSVRDNLILAGQEHRGSMLSRLFGPSD EEECCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCC AGLTETADQMIGFFKLEHLANEPAGGLSYGQQKLLDAAMAFMGGPRLVLLDEPAGGVNPT CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH MLGDLKERLIAINRERRATFVVIEHNMEFVMSLCSRVMVMAEGKVLAIGKPDEIRANPAV HHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHH VEAYLGH HHHCCCC >Mature Secondary Structure TAVLDVRDVKKSFGGITAVDGVSFDVQEGEILGLIGPNGCGKSTLFNCILGQLVPSAGE CCEEHHHHHHHHHCCEEEECCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCC VRVDGKLVTGQRPAELNRLGVSRTFQLLQVFPKLSVRDNLILAGQEHRGSMLSRLFGPSD EEECCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCC AGLTETADQMIGFFKLEHLANEPAGGLSYGQQKLLDAAMAFMGGPRLVLLDEPAGGVNPT CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH MLGDLKERLIAINRERRATFVVIEHNMEFVMSLCSRVMVMAEGKVLAIGKPDEIRANPAV HHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHH VEAYLGH HHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]
Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 7.0
TargetDB status: NA
Availability: NA
References: 2120183; 10984043 [H]