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The map label for this gene is braE [H]

Identifier: 146342436

GI number: 146342436

Start: 5778810

End: 5779808

Strand: Reverse

Name: braE [H]

Synonym: BRADO5595

Alternate gene names: 146342436

Gene position: 5779808-5778810 (Counterclockwise)

Preceding gene: 146342437

Following gene: 146342435

Centisome position: 77.51

GC content: 63.66

Gene sequence:

>999_bases
ATGAGCAAGCATAGCAGACTGCTCCTCGCCGCGCTGGCGGTCGTCGTCATTGCCGGCCTGATCATCATCCCGATGAACTT
CAACCGCTATGGCCTGTACATCTTGAGCCAATGGGCTGTCATGACCATCGCCGCGATGGGGCTCAATTTGACGCTCGGCT
ATGCCGGCCAGGTGTCGCTGGCGCAGGGCGCCTTCGTCGGCATGGGTGCGTATGGCGCCGCCATCCTGACCACCCACGGC
TGGCCGCTGATCGCGGCCCTCGGGCTCGTCATCGTGATCTGCTTCATCGTCGGCTGGCTGCTCGGTTATCCGGCGCTGCG
CGTGCAGCATCACTATCTCGCTTTCGTGACGCTAGCGTTCTCGACCCTGATGTTCCTGGTGTTCCGCAACGAGACCTGGC
TGACCAACGGCATCTATGGCATCAGCAACATCCCGCGTCCCGAGGTCTTTGGCTTTCCGACCCGCAAGCCACTGCCGTTC
TACTACCTCTGCCTCGGCTCGCTCGCGCTGGTGACGCTTGCGATGTGGTGGCTGATCCGCTCGCCCTGGGGCCGCGCCTT
CATGGCGCTGCGCGAGAATCCAATCCGCGCGCAGTCGCTCGGCATCGACACCCGCCGCTACACCTTGATGGCCTTCGCGA
TCGGCTCTGTGCTCGGCGGCATCGCCGGCGTGCTCTATGCGCCGCTCACGCAATATGTCGATCCCGTCCCGTTCAACCTG
CAGCTCTCGCTCGATCTCTTGATGATGGTGATCGTCGGCGGGGCCGGCTTCATGCTCGGACCATTCCTCGGGGCGATGAT
CGCGGTGCTGCTGCCGGAATGGCTGCGCGTGGCCGAGGGTTACTATCTGATGCTCTATGCGATCGCGGTCATCGCGCTGC
TGATCTACTCGCCGACCGGCATTCTCGGCATCCTCGATCGTTATCTCAGCTCACGCCGGACACAGGCGGCGTCCGCGTTG
CGCGCAGCGGCGCAATCCAAGCTGGAGACCGCGCAATGA

Upstream 100 bases:

>100_bases
TCTCCACGCAGTATCGCGCCGCCGTTCCGATGGTTCTGCTGATCCTGGTCATCCTGTTCCGGCCGCAGGGCCTGCTCGGT
CGGGCCGAGGAGCGCACGGT

Downstream 100 bases:

>100_bases
CCGCAGTGCTCGATGTCCGCGACGTCAAGAAGAGCTTCGGCGGCATCACCGCCGTCGACGGCGTCAGCTTCGACGTGCAG
GAGGGCGAGATCCTCGGCCT

Product: branched chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSKHSRLLLAALAVVVIAGLIIIPMNFNRYGLYILSQWAVMTIAAMGLNLTLGYAGQVSLAQGAFVGMGAYGAAILTTHG
WPLIAALGLVIVICFIVGWLLGYPALRVQHHYLAFVTLAFSTLMFLVFRNETWLTNGIYGISNIPRPEVFGFPTRKPLPF
YYLCLGSLALVTLAMWWLIRSPWGRAFMALRENPIRAQSLGIDTRRYTLMAFAIGSVLGGIAGVLYAPLTQYVDPVPFNL
QLSLDLLMMVIVGGAGFMLGPFLGAMIAVLLPEWLRVAEGYYLMLYAIAVIALLIYSPTGILGILDRYLSSRRTQAASAL
RAAAQSKLETAQ

Sequences:

>Translated_332_residues
MSKHSRLLLAALAVVVIAGLIIIPMNFNRYGLYILSQWAVMTIAAMGLNLTLGYAGQVSLAQGAFVGMGAYGAAILTTHG
WPLIAALGLVIVICFIVGWLLGYPALRVQHHYLAFVTLAFSTLMFLVFRNETWLTNGIYGISNIPRPEVFGFPTRKPLPF
YYLCLGSLALVTLAMWWLIRSPWGRAFMALRENPIRAQSLGIDTRRYTLMAFAIGSVLGGIAGVLYAPLTQYVDPVPFNL
QLSLDLLMMVIVGGAGFMLGPFLGAMIAVLLPEWLRVAEGYYLMLYAIAVIALLIYSPTGILGILDRYLSSRRTQAASAL
RAAAQSKLETAQ
>Mature_331_residues
SKHSRLLLAALAVVVIAGLIIIPMNFNRYGLYILSQWAVMTIAAMGLNLTLGYAGQVSLAQGAFVGMGAYGAAILTTHGW
PLIAALGLVIVICFIVGWLLGYPALRVQHHYLAFVTLAFSTLMFLVFRNETWLTNGIYGISNIPRPEVFGFPTRKPLPFY
YLCLGSLALVTLAMWWLIRSPWGRAFMALRENPIRAQSLGIDTRRYTLMAFAIGSVLGGIAGVLYAPLTQYVDPVPFNLQ
LSLDLLMMVIVGGAGFMLGPFLGAMIAVLLPEWLRVAEGYYLMLYAIAVIALLIYSPTGILGILDRYLSSRRTQAASALR
AAAQSKLETAQ

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=303, Percent_Identity=30.6930693069307, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36244; Mature: 36113

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHSRLLLAALAVVVIAGLIIIPMNFNRYGLYILSQWAVMTIAAMGLNLTLGYAGQVSL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEH
AQGAFVGMGAYGAAILTTHGWPLIAALGLVIVICFIVGWLLGYPALRVQHHYLAFVTLAF
HCCCCEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
STLMFLVFRNETWLTNGIYGISNIPRPEVFGFPTRKPLPFYYLCLGSLALVTLAMWWLIR
HHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SPWGRAFMALRENPIRAQSLGIDTRRYTLMAFAIGSVLGGIAGVLYAPLTQYVDPVPFNL
CCHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
QLSLDLLMMVIVGGAGFMLGPFLGAMIAVLLPEWLRVAEGYYLMLYAIAVIALLIYSPTG
EHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCH
ILGILDRYLSSRRTQAASALRAAAQSKLETAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKHSRLLLAALAVVVIAGLIIIPMNFNRYGLYILSQWAVMTIAAMGLNLTLGYAGQVSL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEH
AQGAFVGMGAYGAAILTTHGWPLIAALGLVIVICFIVGWLLGYPALRVQHHYLAFVTLAF
HCCCCEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
STLMFLVFRNETWLTNGIYGISNIPRPEVFGFPTRKPLPFYYLCLGSLALVTLAMWWLIR
HHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SPWGRAFMALRENPIRAQSLGIDTRRYTLMAFAIGSVLGGIAGVLYAPLTQYVDPVPFNL
CCHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
QLSLDLLMMVIVGGAGFMLGPFLGAMIAVLLPEWLRVAEGYYLMLYAIAVIALLIYSPTG
EHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCH
ILGILDRYLSSRRTQAASALRAAAQSKLETAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]