BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is livF [H]

Identifier: 146342434

GI number: 146342434

Start: 5777321

End: 5778058

Strand: Reverse

Name: livF [H]

Synonym: BRADO5593

Alternate gene names: 146342434

Gene position: 5778058-5777321 (Counterclockwise)

Preceding gene: 146342435

Following gene: 146342433

Centisome position: 77.49

GC content: 65.72

Gene sequence:

>738_bases
GTGAGCGAACCCATTCTCGATGTCCAGAACCTGGTCGGCGGCTACGGCAAGATGACGATCCTGAACGGCACGACCTTTTC
GGTGCCGAAGGGATCGATCACCACCGTCATCGGTCCGAACGGCGCCGGCAAGTCGACCGTGTTCAAGGCGATCTTCGGGC
TTCTCAAGCTCCGCGAAGGCAAGGTCGTATTCAAGGGCCGTGATGTCACGGGCCTCAGCCAGCGCGCGCTGCTCGGCGCC
GGCATCTGCTACATTCCGCAGGGCCGCAACATCTTCGGCGAGCTGTCGGTGCGCCACAACATCGAGCTCGGCGGCGTGGC
CGCGCCGGCCAACATCGATCTGCCGGCTCGGGTCGAGGCGGCGCTCGACGTGTTTCCGGTGCTGCGCAAGAAGGCGGATC
GGCAGGCCTCGACACTGTCGGGCGGCGAGCAGAAGCAGCTCGAGATCGCGCGCGGTCTCTTGCTGGAGCCGCAGCTCGTG
CTGATCGACGAGCCTTCGATCGGGCTGTCGCCGCTGATGGTGCAGCAGACCTTCGACATGCTGAAGCAGCTGCGCGACAA
GGGCGTGTCGGTGCTGCTGATCGAGCAGAACGCGCGCTCGGCTTTGGAGATCTCCGACTACGGCATCGTCCTGGAGCTCG
GCCGCACCCGGATCGTCGACAAGGCTGATCGCGTGCTGGCCGATCCGCGCATCGGACAGCTGTTCCTGGGCGGCGCGATG
GAGGAGACGGCGGCATGA

Upstream 100 bases:

>100_bases
TCATGGCGGAAGGCAAGGTCTTGGCGATCGGCAAGCCGGACGAGATCAGGGCCAATCCGGCCGTGGTCGAAGCCTATCTC
GGACACTGACGGAGGCGGCC

Downstream 100 bases:

>100_bases
ACCAGCGCTCGATCGCAACGGTCTCCATCTCAGGTGCCCTCGACGAGAAGCTCAAGGCGATCGCCGCGGCCGGCTTCGAA
CTGGTCGAGATCTTCGAAAA

Product: putative branched-chain amino acid ABC transporter ATP-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein F [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSEPILDVQNLVGGYGKMTILNGTTFSVPKGSITTVIGPNGAGKSTVFKAIFGLLKLREGKVVFKGRDVTGLSQRALLGA
GICYIPQGRNIFGELSVRHNIELGGVAAPANIDLPARVEAALDVFPVLRKKADRQASTLSGGEQKQLEIARGLLLEPQLV
LIDEPSIGLSPLMVQQTFDMLKQLRDKGVSVLLIEQNARSALEISDYGIVLELGRTRIVDKADRVLADPRIGQLFLGGAM
EETAA

Sequences:

>Translated_245_residues
MSEPILDVQNLVGGYGKMTILNGTTFSVPKGSITTVIGPNGAGKSTVFKAIFGLLKLREGKVVFKGRDVTGLSQRALLGA
GICYIPQGRNIFGELSVRHNIELGGVAAPANIDLPARVEAALDVFPVLRKKADRQASTLSGGEQKQLEIARGLLLEPQLV
LIDEPSIGLSPLMVQQTFDMLKQLRDKGVSVLLIEQNARSALEISDYGIVLELGRTRIVDKADRVLADPRIGQLFLGGAM
EETAA
>Mature_244_residues
SEPILDVQNLVGGYGKMTILNGTTFSVPKGSITTVIGPNGAGKSTVFKAIFGLLKLREGKVVFKGRDVTGLSQRALLGAG
ICYIPQGRNIFGELSVRHNIELGGVAAPANIDLPARVEAALDVFPVLRKKADRQASTLSGGEQKQLEIARGLLLEPQLVL
IDEPSIGLSPLMVQQTFDMLKQLRDKGVSVLLIEQNARSALEISDYGIVLELGRTRIVDKADRVLADPRIGQLFLGGAME
ETAA

Specific function: Component of the leucine-specific transport system [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=175, Percent_Identity=29.7142857142857, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI27436953, Length=213, Percent_Identity=28.6384976525822, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI31657092, Length=206, Percent_Identity=26.6990291262136, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI150417984, Length=226, Percent_Identity=28.3185840707965, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI6005701, Length=197, Percent_Identity=29.9492385786802, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI42490749, Length=216, Percent_Identity=25, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI27477115, Length=183, Percent_Identity=30.0546448087432, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI9955963, Length=236, Percent_Identity=27.9661016949153, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI21536376, Length=219, Percent_Identity=27.3972602739726, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI47078218, Length=221, Percent_Identity=26.2443438914027, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI45446740, Length=221, Percent_Identity=26.2443438914027, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI105990541, Length=187, Percent_Identity=27.807486631016, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI27262624, Length=178, Percent_Identity=29.2134831460674, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI27262626, Length=178, Percent_Identity=29.2134831460674, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI9665248, Length=207, Percent_Identity=28.9855072463768, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI148612844, Length=208, Percent_Identity=27.8846153846154, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI255708477, Length=208, Percent_Identity=27.8846153846154, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI171184400, Length=239, Percent_Identity=26.7782426778243, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI87082268, Length=238, Percent_Identity=35.7142857142857, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1789864, Length=256, Percent_Identity=28.125, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789593, Length=237, Percent_Identity=29.535864978903, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI87081709, Length=239, Percent_Identity=27.1966527196653, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1788761, Length=230, Percent_Identity=27.3913043478261, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1790162, Length=235, Percent_Identity=28.0851063829787, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788225, Length=238, Percent_Identity=27.7310924369748, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789672, Length=237, Percent_Identity=27.8481012658228, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786872, Length=229, Percent_Identity=27.9475982532751, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1786398, Length=238, Percent_Identity=30.2521008403361, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790190, Length=244, Percent_Identity=26.2295081967213, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI48994943, Length=223, Percent_Identity=28.6995515695067, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1788472, Length=216, Percent_Identity=25.9259259259259, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081782, Length=212, Percent_Identity=27.8301886792453, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790525, Length=225, Percent_Identity=28, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994997, Length=238, Percent_Identity=27.3109243697479, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789873, Length=196, Percent_Identity=33.1632653061224, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787089, Length=220, Percent_Identity=28.1818181818182, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787029, Length=215, Percent_Identity=24.6511627906977, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787792, Length=225, Percent_Identity=26.2222222222222, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788644, Length=261, Percent_Identity=28.3524904214559, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081834, Length=218, Percent_Identity=29.3577981651376, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790467, Length=232, Percent_Identity=27.5862068965517, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1787370, Length=216, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788210, Length=223, Percent_Identity=26.457399103139, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789586, Length=245, Percent_Identity=26.9387755102041, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI48995001, Length=228, Percent_Identity=28.0701754385965, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787712, Length=227, Percent_Identity=26.431718061674, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789991, Length=229, Percent_Identity=25.764192139738, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786319, Length=191, Percent_Identity=31.9371727748691, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787105, Length=222, Percent_Identity=26.5765765765766, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786698, Length=212, Percent_Identity=26.4150943396226, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081791, Length=239, Percent_Identity=23.8493723849372, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788897, Length=205, Percent_Identity=27.3170731707317, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1790739, Length=233, Percent_Identity=27.8969957081545, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788450, Length=207, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786563, Length=220, Percent_Identity=26.8181818181818, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790544, Length=269, Percent_Identity=24.5353159851301, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI145693107, Length=234, Percent_Identity=28.6324786324786, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788165, Length=212, Percent_Identity=27.8301886792453, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789962, Length=221, Percent_Identity=26.2443438914027, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789032, Length=212, Percent_Identity=26.4150943396226, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI2367384, Length=172, Percent_Identity=29.6511627906977, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786253, Length=199, Percent_Identity=26.6331658291457, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082267, Length=203, Percent_Identity=24.6305418719212, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788053, Length=168, Percent_Identity=31.547619047619, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788506, Length=219, Percent_Identity=24.2009132420091, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787972, Length=245, Percent_Identity=27.7551020408163, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786345, Length=205, Percent_Identity=25.3658536585366, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786981, Length=201, Percent_Identity=29.3532338308458, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=217, Percent_Identity=26.7281105990783, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17565586, Length=189, Percent_Identity=28.042328042328, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17510237, Length=200, Percent_Identity=28.5, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71983510, Length=199, Percent_Identity=26.1306532663317, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71983516, Length=199, Percent_Identity=26.1306532663317, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71983523, Length=199, Percent_Identity=26.1306532663317, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115534520, Length=226, Percent_Identity=26.9911504424779, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI115533592, Length=189, Percent_Identity=29.6296296296296, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17539902, Length=208, Percent_Identity=28.8461538461538, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193208177, Length=234, Percent_Identity=25.6410256410256, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323959, Length=239, Percent_Identity=28.4518828451883, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI85725262, Length=179, Percent_Identity=30.7262569832402, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI221512771, Length=213, Percent_Identity=29.5774647887324, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24650855, Length=212, Percent_Identity=26.4150943396226, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24650853, Length=212, Percent_Identity=26.4150943396226, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI281362751, Length=212, Percent_Identity=26.4150943396226, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24643674, Length=182, Percent_Identity=29.1208791208791, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24666092, Length=215, Percent_Identity=25.5813953488372, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21357907, Length=170, Percent_Identity=30, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648314, Length=215, Percent_Identity=26.9767441860465, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24582014, Length=216, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582012, Length=216, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI85724864, Length=193, Percent_Identity=30.5699481865285, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580555, Length=193, Percent_Identity=30.5699481865285, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI85816269, Length=193, Percent_Identity=30.5699481865285, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580553, Length=193, Percent_Identity=30.5699481865285, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574533, Length=208, Percent_Identity=26.4423076923077, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24662800, Length=212, Percent_Identity=29.2452830188679, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI161076684, Length=210, Percent_Identity=28.0952380952381, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581617, Length=210, Percent_Identity=28.0952380952381, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581615, Length=210, Percent_Identity=28.0952380952381, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581191, Length=207, Percent_Identity=26.5700483091787, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24581189, Length=207, Percent_Identity=26.5700483091787, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24581193, Length=207, Percent_Identity=26.5700483091787, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24581195, Length=207, Percent_Identity=26.5700483091787, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28573573, Length=219, Percent_Identity=25.5707762557078, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI28573571, Length=219, Percent_Identity=25.5707762557078, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI221500365, Length=222, Percent_Identity=27.027027027027, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI116007184, Length=222, Percent_Identity=27.027027027027, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26201; Mature: 26070

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPILDVQNLVGGYGKMTILNGTTFSVPKGSITTVIGPNGAGKSTVFKAIFGLLKLREG
CCCCCHHHHHHHCCCCEEEEECCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC
KVVFKGRDVTGLSQRALLGAGICYIPQGRNIFGELSVRHNIELGGVAAPANIDLPARVEA
CEEEECCCCCCCHHHHHHHCCEEEECCCCCEEEEEEEEECCEECCEECCCCCCCCHHHHH
ALDVFPVLRKKADRQASTLSGGEQKQLEIARGLLLEPQLVLIDEPSIGLSPLMVQQTFDM
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCEECCCEEEEEECCCCCCCHHHHHHHHHH
LKQLRDKGVSVLLIEQNARSALEISDYGIVLELGRTRIVDKADRVLADPRIGQLFLGGAM
HHHHHHCCCEEEEEECCCCCEEEECCCEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCH
EETAA
HHHCC
>Mature Secondary Structure 
SEPILDVQNLVGGYGKMTILNGTTFSVPKGSITTVIGPNGAGKSTVFKAIFGLLKLREG
CCCCHHHHHHHCCCCEEEEECCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC
KVVFKGRDVTGLSQRALLGAGICYIPQGRNIFGELSVRHNIELGGVAAPANIDLPARVEA
CEEEECCCCCCCHHHHHHHCCEEEECCCCCEEEEEEEEECCEECCEECCCCCCCCHHHHH
ALDVFPVLRKKADRQASTLSGGEQKQLEIARGLLLEPQLVLIDEPSIGLSPLMVQQTFDM
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCEECCCEEEEEECCCCCCCHHHHHHHHHH
LKQLRDKGVSVLLIEQNARSALEISDYGIVLELGRTRIVDKADRVLADPRIGQLFLGGAM
HHHHHHCCCEEEEEECCCCCEEEECCCEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCH
EETAA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]