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The map label for this gene is livK [C]

Identifier: 146342357

GI number: 146342357

Start: 5695249

End: 5696472

Strand: Reverse

Name: livK [C]

Synonym: BRADO5513

Alternate gene names: 146342357

Gene position: 5696472-5695249 (Counterclockwise)

Preceding gene: 146342358

Following gene: 146342356

Centisome position: 76.4

GC content: 64.79

Gene sequence:

>1224_bases
ATGACGTTGAGCTCGAAGTCACTGTCACGCCGCACCGTGCTCAAAAGCGGTGTCGCGCTCACCTCGATGCTGGCGGCGCC
TGGCCTGCTGCGCGCCGAGCCGGCGCCGGTCAAGGTCGGCCTGCTGCAGCCGATCTCCGGCGCCTTCGCGCTCGACGGCG
ATCTCGCCAAGATCGGCGCGGAGTTCGCGATCAAGGAGATCAACGACGCCGGCGGCATCAAGGCGCTGGGCGGCGCCAAG
CTCGAGCTTATCGTCGGCGACAGCCGCTCGAACGCGGAGGCCGGCGCGCAGGCGACCGAGGAGTTGCAATCGGCCGGCGT
GGCCGCCGTGCTCGGCGGCTTTGCCTCGGGCATTGCTCTGACCGCCACGCAAACCGCCTCCCGCTACGATCTGCCCTTCG
TGGTCGACTGTGCGGTGGCGGATACGATCACCGAGCGCGGCCTCAAGAACACCTTCCGCTTCAATCCGAATTTCAGCATG
GCGACCGCGGTCGCGCTGAAGAACCTCGTCAAGCTCAACGACGACGCCGGCAAGCCGATCAAGACGGTGGCGATCGTGCA
CGAAGACGGCCTCTTCGGCTCGGGCCTCGCCAAGATCATGCAGGAGAAGCTGCCGCCGCTCGGCTTCCAGATCGTCGAGA
CCATCGCGCATCCGACCCCGGCGCGCGACATGACCAACGTCGTGCTGCGGCTGCGCGCGCTCAATCCAGATCTGATCGTG
CCGTCGCATTACTTCAACGAGTTCGTGCTGATGGCCCGCACTCTGCAGCAGCAGCGCGTGCGCCCGAAGGGCATTTACGC
CGTGTTCGGCGGCGCGGCATCGAGCTATCGCTTCGTGAACGAGTTCCCCGAGGCTGCCCAGGGCGTGATGGACTGCAACC
ATTGGGGTGATCCGAAGAGCCCGATCACGGCCAAGCTGCGCGAGACCGTGACCGCTGCCGGCAAGTTCTACGCCTACAAT
ACGCCGATCAACTATTCGCTGGTGAAGGTGTTCGCTCAGGCTGTCGAGAATGCCGGCAGCGCCGATCGTGCCAAGATCAT
CGAGGCGCTGGCAGCGAACGAATTCGACAGCGGCGTCATGCCCTATGGCAAGACCAAGTTCGACGCCAAAGGCCAGAACA
CCAGCGCACTGCCGCTGAACACGCAGGTGCAAGGCAAGGACATCAAAGTGATCTACCCGGCGGACTACGCCGACGCAAAG
CCGGTGTTCCCGATCAACGGGTGA

Upstream 100 bases:

>100_bases
GGACGGGCGACGAAGGGCACCGGCACTGGAGCCCGACCGCCGAGGTGTACCGCAGGGAGCGCCCGCGCCGGCGGCCGCCA
GTCAGGAAAGTGGGGAAGTC

Downstream 100 bases:

>100_bases
TCGGGGGGCACGACTCCCGCTTCGGTAAAAAAGCCGTGGCGATTGCGGTAGACTTGGCCATCCGTCGAGACGCCCGGCCT
CGCCGGGCTCCTCAGGATCA

Product: putative leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MTLSSKSLSRRTVLKSGVALTSMLAAPGLLRAEPAPVKVGLLQPISGAFALDGDLAKIGAEFAIKEINDAGGIKALGGAK
LELIVGDSRSNAEAGAQATEELQSAGVAAVLGGFASGIALTATQTASRYDLPFVVDCAVADTITERGLKNTFRFNPNFSM
ATAVALKNLVKLNDDAGKPIKTVAIVHEDGLFGSGLAKIMQEKLPPLGFQIVETIAHPTPARDMTNVVLRLRALNPDLIV
PSHYFNEFVLMARTLQQQRVRPKGIYAVFGGAASSYRFVNEFPEAAQGVMDCNHWGDPKSPITAKLRETVTAAGKFYAYN
TPINYSLVKVFAQAVENAGSADRAKIIEALAANEFDSGVMPYGKTKFDAKGQNTSALPLNTQVQGKDIKVIYPADYADAK
PVFPING

Sequences:

>Translated_407_residues
MTLSSKSLSRRTVLKSGVALTSMLAAPGLLRAEPAPVKVGLLQPISGAFALDGDLAKIGAEFAIKEINDAGGIKALGGAK
LELIVGDSRSNAEAGAQATEELQSAGVAAVLGGFASGIALTATQTASRYDLPFVVDCAVADTITERGLKNTFRFNPNFSM
ATAVALKNLVKLNDDAGKPIKTVAIVHEDGLFGSGLAKIMQEKLPPLGFQIVETIAHPTPARDMTNVVLRLRALNPDLIV
PSHYFNEFVLMARTLQQQRVRPKGIYAVFGGAASSYRFVNEFPEAAQGVMDCNHWGDPKSPITAKLRETVTAAGKFYAYN
TPINYSLVKVFAQAVENAGSADRAKIIEALAANEFDSGVMPYGKTKFDAKGQNTSALPLNTQVQGKDIKVIYPADYADAK
PVFPING
>Mature_406_residues
TLSSKSLSRRTVLKSGVALTSMLAAPGLLRAEPAPVKVGLLQPISGAFALDGDLAKIGAEFAIKEINDAGGIKALGGAKL
ELIVGDSRSNAEAGAQATEELQSAGVAAVLGGFASGIALTATQTASRYDLPFVVDCAVADTITERGLKNTFRFNPNFSMA
TAVALKNLVKLNDDAGKPIKTVAIVHEDGLFGSGLAKIMQEKLPPLGFQIVETIAHPTPARDMTNVVLRLRALNPDLIVP
SHYFNEFVLMARTLQQQRVRPKGIYAVFGGAASSYRFVNEFPEAAQGVMDCNHWGDPKSPITAKLRETVTAAGKFYAYNT
PINYSLVKVFAQAVENAGSADRAKIIEALAANEFDSGVMPYGKTKFDAKGQNTSALPLNTQVQGKDIKVIYPADYADAKP
VFPING

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=341, Percent_Identity=24.9266862170088, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI48994941, Length=256, Percent_Identity=26.171875, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 43143; Mature: 43012

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSSKSLSRRTVLKSGVALTSMLAAPGLLRAEPAPVKVGLLQPISGAFALDGDLAKIGA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEECCCHHHHHH
EFAIKEINDAGGIKALGGAKLELIVGDSRSNAEAGAQATEELQSAGVAAVLGGFASGIAL
HHHHHHHCCCCCEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCEEE
TATQTASRYDLPFVVDCAVADTITERGLKNTFRFNPNFSMATAVALKNLVKLNDDAGKPI
EEECCCCCCCCCEEEEHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCE
KTVAIVHEDGLFGSGLAKIMQEKLPPLGFQIVETIAHPTPARDMTNVVLRLRALNPDLIV
EEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHEECCCCEEC
PSHYFNEFVLMARTLQQQRVRPKGIYAVFGGAASSYRFVNEFPEAAQGVMDCNHWGDPKS
CHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
PITAKLRETVTAAGKFYAYNTPINYSLVKVFAQAVENAGSADRAKIIEALAANEFDSGVM
CHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
PYGKTKFDAKGQNTSALPLNTQVQGKDIKVIYPADYADAKPVFPING
CCCCCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLSSKSLSRRTVLKSGVALTSMLAAPGLLRAEPAPVKVGLLQPISGAFALDGDLAKIGA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEECCCHHHHHH
EFAIKEINDAGGIKALGGAKLELIVGDSRSNAEAGAQATEELQSAGVAAVLGGFASGIAL
HHHHHHHCCCCCEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCEEE
TATQTASRYDLPFVVDCAVADTITERGLKNTFRFNPNFSMATAVALKNLVKLNDDAGKPI
EEECCCCCCCCCEEEEHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCE
KTVAIVHEDGLFGSGLAKIMQEKLPPLGFQIVETIAHPTPARDMTNVVLRLRALNPDLIV
EEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHEECCCCEEC
PSHYFNEFVLMARTLQQQRVRPKGIYAVFGGAASSYRFVNEFPEAAQGVMDCNHWGDPKS
CHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
PITAKLRETVTAAGKFYAYNTPINYSLVKVFAQAVENAGSADRAKIIEALAANEFDSGVM
CHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
PYGKTKFDAKGQNTSALPLNTQVQGKDIKVIYPADYADAKPVFPING
CCCCCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA