BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is fixR [H]

Identifier: 146342295

GI number: 146342295

Start: 5638081

End: 5638926

Strand: Reverse

Name: fixR [H]

Synonym: BRADO5450

Alternate gene names: 146342295

Gene position: 5638926-5638081 (Counterclockwise)

Preceding gene: 146342298

Following gene: 146342294

Centisome position: 75.62

GC content: 66.19

Gene sequence:

>846_bases
GTGAGCCTCGATCTCGACCAGGATGTTCGCGAGGGCGCCATCGACACGGCGGAGATCGAGTCGTGCCCGTTTCATCGGGA
CGCTGCGGCGGCCGAGCGTCCCGGACGCACCGAGCAGAAGACGCTGGTTCTGACCGGCGGCTCGCGCGGCATCGGCCACG
CGACGGCCCGGCTGTTTTCCGATGCCGGCTGGCGTATCATCACCTGCTCGCGCCAGCCGTTCGATGGCGGGCGCTGTCCC
TGGGATGCCGGGCCGGACAATCACGTTCAGGTCGATCTGAGCGACCGCAAGGCGATCCCGCGCGCGATCGCGGATATCAA
GGACCGGCTGGACGGCGCGCCGCTCCATGCCTTGATCAACAATGCCGCGATCTCGCCGAAGGCTGAGGATGGCGGCCGGC
TCGATTCGCTCGCGACCTCGGTCGAGACCTGGATGACGGTCTTTCACGTCAACTTTCTCGGGCCCATCCTGTTGGCGCGC
GGCCTGTTCCAGGAACTGAAACGCGGCCAGGGCGCGGTCGTCAACGTGACCTCGATCGTCGGTACCCGCGTGCACCCGTT
TGCCGGCACGGCCTATGCGACATCGAAGGCGGCGCTGGCCTGCCTGACCCGCGAGATGGCGCATGATTTCGCGCCGCATG
GCATCCGCGTGAACGCGATCGCACCCGGCGAAATCAAGACCGAAATGGTGTCGCCGGAGACCGAGGCGCGATTCTCACCG
TTGATCCCGATGCGCCGGATCGGCGCACCGGAAGAGGTCGCGAAAGTGCTGTTCTTCCTCTGCTCGGATGCGGCCAGCTA
TGTGACCGGCGAGGAAATCCAGATCAACGGAGGGCAGCACCTCTAG

Upstream 100 bases:

>100_bases
AATCGCGCCGTTTTTTCTCTGGCCCGGAATCTGCACAACCGCCGCATGACAATACCGCGCGTGGTTTCAGCGAGCGCGGT
GCAGGATCAGGACGAATGAG

Downstream 100 bases:

>100_bases
ATTGAATTGAGCGACACGCCGATGTGAGACGTGCGACGTCAAAAGCTGGCAGCCGCATGCGGCGAGTGCTACGGCGGATG
TGGAAAGTAGGTGTTCAGTT

Product: short-chain dehydrogenase FixR

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSLDLDQDVREGAIDTAEIESCPFHRDAAAAERPGRTEQKTLVLTGGSRGIGHATARLFSDAGWRIITCSRQPFDGGRCP
WDAGPDNHVQVDLSDRKAIPRAIADIKDRLDGAPLHALINNAAISPKAEDGGRLDSLATSVETWMTVFHVNFLGPILLAR
GLFQELKRGQGAVVNVTSIVGTRVHPFAGTAYATSKAALACLTREMAHDFAPHGIRVNAIAPGEIKTEMVSPETEARFSP
LIPMRRIGAPEEVAKVLFFLCSDAASYVTGEEIQINGGQHL

Sequences:

>Translated_281_residues
MSLDLDQDVREGAIDTAEIESCPFHRDAAAAERPGRTEQKTLVLTGGSRGIGHATARLFSDAGWRIITCSRQPFDGGRCP
WDAGPDNHVQVDLSDRKAIPRAIADIKDRLDGAPLHALINNAAISPKAEDGGRLDSLATSVETWMTVFHVNFLGPILLAR
GLFQELKRGQGAVVNVTSIVGTRVHPFAGTAYATSKAALACLTREMAHDFAPHGIRVNAIAPGEIKTEMVSPETEARFSP
LIPMRRIGAPEEVAKVLFFLCSDAASYVTGEEIQINGGQHL
>Mature_280_residues
SLDLDQDVREGAIDTAEIESCPFHRDAAAAERPGRTEQKTLVLTGGSRGIGHATARLFSDAGWRIITCSRQPFDGGRCPW
DAGPDNHVQVDLSDRKAIPRAIADIKDRLDGAPLHALINNAAISPKAEDGGRLDSLATSVETWMTVFHVNFLGPILLARG
LFQELKRGQGAVVNVTSIVGTRVHPFAGTAYATSKAALACLTREMAHDFAPHGIRVNAIAPGEIKTEMVSPETEARFSPL
IPMRRIGAPEEVAKVLFFLCSDAASYVTGEEIQINGGQHL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=254, Percent_Identity=31.8897637795276, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI40254992, Length=244, Percent_Identity=29.9180327868852, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI5031737, Length=260, Percent_Identity=31.1538461538462, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI15277342, Length=265, Percent_Identity=29.0566037735849, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI19923817, Length=268, Percent_Identity=25.7462686567164, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI7705925, Length=249, Percent_Identity=30.1204819277108, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI126723750, Length=173, Percent_Identity=34.1040462427746, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI59889578, Length=235, Percent_Identity=28.0851063829787, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI10190704, Length=261, Percent_Identity=31.4176245210728, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI109715829, Length=260, Percent_Identity=26.9230769230769, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI20149619, Length=206, Percent_Identity=32.5242718446602, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI119392083, Length=203, Percent_Identity=29.064039408867, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI66933014, Length=257, Percent_Identity=27.2373540856031, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI33667109, Length=207, Percent_Identity=30.4347826086957, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4503301, Length=204, Percent_Identity=27.9411764705882, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI40548397, Length=196, Percent_Identity=27.0408163265306, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI214010158, Length=196, Percent_Identity=27.0408163265306, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI214010156, Length=196, Percent_Identity=27.0408163265306, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI22758144, Length=196, Percent_Identity=27.0408163265306, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI2367175, Length=253, Percent_Identity=35.1778656126482, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787335, Length=253, Percent_Identity=29.6442687747036, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI87082100, Length=270, Percent_Identity=31.4814814814815, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788459, Length=256, Percent_Identity=32.03125, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786812, Length=257, Percent_Identity=29.9610894941634, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI2367365, Length=242, Percent_Identity=32.2314049586777, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787905, Length=262, Percent_Identity=29.0076335877863, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790717, Length=249, Percent_Identity=28.5140562248996, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789378, Length=260, Percent_Identity=28.4615384615385, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87082160, Length=253, Percent_Identity=28.4584980237154, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789208, Length=265, Percent_Identity=27.1698113207547, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786701, Length=195, Percent_Identity=30.2564102564103, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787891, Length=255, Percent_Identity=29.0196078431373, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=248, Percent_Identity=31.8548387096774, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560676, Length=252, Percent_Identity=32.5396825396825, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71994600, Length=255, Percent_Identity=32.156862745098, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17560332, Length=268, Percent_Identity=29.8507462686567, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI25147288, Length=248, Percent_Identity=30.241935483871, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI115534694, Length=255, Percent_Identity=28.2352941176471, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71994604, Length=253, Percent_Identity=30.8300395256917, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI72000259, Length=272, Percent_Identity=29.4117647058824, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562910, Length=267, Percent_Identity=28.0898876404494, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562990, Length=270, Percent_Identity=30, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560150, Length=272, Percent_Identity=29.7794117647059, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17538480, Length=268, Percent_Identity=28.3582089552239, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559104, Length=273, Percent_Identity=27.8388278388278, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17531453, Length=269, Percent_Identity=28.6245353159851, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17563726, Length=266, Percent_Identity=28.1954887218045, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17561402, Length=261, Percent_Identity=28.3524904214559, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17562906, Length=268, Percent_Identity=29.1044776119403, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17565030, Length=272, Percent_Identity=27.9411764705882, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562908, Length=268, Percent_Identity=28.7313432835821, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17544670, Length=285, Percent_Identity=27.3684210526316, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17551412, Length=270, Percent_Identity=27.4074074074074, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562904, Length=277, Percent_Identity=27.7978339350181, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17538486, Length=272, Percent_Identity=27.5735294117647, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17536651, Length=278, Percent_Identity=27.3381294964029, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17561272, Length=208, Percent_Identity=31.25, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17536025, Length=282, Percent_Identity=25.886524822695, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=267, Percent_Identity=26.5917602996255, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17508651, Length=252, Percent_Identity=25.3968253968254, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=201, Percent_Identity=28.3582089552239, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI23397609, Length=256, Percent_Identity=33.59375, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24639444, Length=247, Percent_Identity=30.3643724696356, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21355319, Length=259, Percent_Identity=33.5907335907336, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24643142, Length=249, Percent_Identity=31.7269076305221, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357041, Length=260, Percent_Identity=31.5384615384615, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571526, Length=273, Percent_Identity=29.3040293040293, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24644337, Length=256, Percent_Identity=32.03125, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24644339, Length=261, Percent_Identity=28.735632183908, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21358495, Length=199, Percent_Identity=28.1407035175879, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI17737361, Length=250, Percent_Identity=27.6, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 30213; Mature: 30081

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDLDQDVREGAIDTAEIESCPFHRDAAAAERPGRTEQKTLVLTGGSRGIGHATARLFS
CCCCCCCHHHHCCCCHHHHCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHH
DAGWRIITCSRQPFDGGRCPWDAGPDNHVQVDLSDRKAIPRAIADIKDRLDGAPLHALIN
CCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHH
NAAISPKAEDGGRLDSLATSVETWMTVFHVNFLGPILLARGLFQELKRGQGAVVNVTSIV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHH
GTRVHPFAGTAYATSKAALACLTREMAHDFAPHGIRVNAIAPGEIKTEMVSPETEARFSP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCCHHCCCCC
LIPMRRIGAPEEVAKVLFFLCSDAASYVTGEEIQINGGQHL
CCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
>Mature Secondary Structure 
SLDLDQDVREGAIDTAEIESCPFHRDAAAAERPGRTEQKTLVLTGGSRGIGHATARLFS
CCCCCCHHHHCCCCHHHHCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHH
DAGWRIITCSRQPFDGGRCPWDAGPDNHVQVDLSDRKAIPRAIADIKDRLDGAPLHALIN
CCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHH
NAAISPKAEDGGRLDSLATSVETWMTVFHVNFLGPILLARGLFQELKRGQGAVVNVTSIV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHH
GTRVHPFAGTAYATSKAALACLTREMAHDFAPHGIRVNAIAPGEIKTEMVSPETEARFSP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCCHHCCCCC
LIPMRRIGAPEEVAKVLFFLCSDAASYVTGEEIQINGGQHL
CCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3313281; 11157954; 12597275 [H]