Click here to switch to the map view.

The map label for this gene is nifA [H]

Identifier: 146342294

GI number: 146342294

Start: 5635928

End: 5637667

Strand: Reverse

Name: nifA [H]

Synonym: BRADO5449

Alternate gene names: 146342294

Gene position: 5637667-5635928 (Counterclockwise)

Preceding gene: 146342295

Following gene: 146342293

Centisome position: 75.61

GC content: 65.63

Gene sequence:

>1740_bases
ATGGTCCAAACGGAAACACGTCAGGTCGAAGCTCAGGACATCAGGTCCCATGCCGCCCCGATACCGCTCAGCGAGATCGC
GCTGACCGGAATTTTCGAAATCTCGAAAATTCTCGCCGCGCCGACGCGGCTGGAAATCACCCTCGCCAACGTCGTCAATC
TGCTGCAGTCGTTCATGCAGATGCGCCACGGCGTGGTGTCGTTGCTCGCCGATGACGGCATTCCGGATCTCGCGGTGGGA
GCCGGTTGGAGCGAGGGCAGCGATCAGCGCTATCGCGAGCGCCTGCCGCAGAAGGCGATCGACCAGATCGTCGCGACCGC
CGTGCCGCTCATTGCTGAGAACGTGGCTGATCATTCCGCCTTCAGCGCGGCTGATGCGGCAACGCTGGGGGCAGGCGCCG
ACACGCGGGTGTCGTTCCTGGGCGTGCCCATCCGGATCGACCAGCGTGTCGTCGGCACCTTGACCATCGATCGCGTCTGG
GACGGTCGCGCCGTGTTCCGGCTCGACTCCGATGTCCGCTTCCTGACGATGGTCGCCAATCTGATCGGCCAGACCATCAA
GCTGCATCGTGTCGTCGCGCGCGACCGCGAGCGGCTGATGGCAGAGAGCCACCGGCTGCAGAAGGAGTTGATGGAGCTGA
AGCCGGCGCCCGCCAAGGGCAAGAAGGTGCTGGTCGACGGTATCGTCGGCGACAGCCCGGCGCTGCGCGCGCTGCTCGAC
AAGATCAACGTCGTCGCCAAGTCGAATGCGCCGGTGCTGCTGCGCGGCGAATCCGGCACCGGCAAGGAGCTTGTGGCGAA
GGCCATCCACGAACTCTCCAACCGCGCCAAGCGGCCCTTCATCAAGATCAATTGCGCGGCGCTACCGGAGACGGTGCTCG
AATCCGAGCTGTTCGGCCACGAGAAGGGGGCCTTCACCGGCGCGATCAACGCGCGCAAAGGTCGCTTCGAGCTCGCCGAC
AAGGGCACCTTGTTCCTCGACGAGATCGGCGAGATCTCGCCGGCGTTCCAGGCCAAGCTGTTGCGCGTGCTGCAGGAGCA
GGAGTTCGAGCGCGTCGGCGGCAACCAGACCATCAAGGTCGATGTCCGCATCGTCACCGCGACCAACCGCAACCTGGAAG
AGGCGGTGGCGCGCAACGAATTCCGTGCCGACCTCTACTACCGGATCAGCGTGGTGCCGATCAAACTGCCGCCGCTGCGC
GATCGGCGCAGCGACATCCCGCAGCTGGCGCGCGAGTTCCTGCGCCGCTTCAATTGCGAGAACGAGCGCGATCTCACCTT
CGACGTCAGCGCCATCGAGGTGCTGATGCATTGCGGCTTCCCCGGCAATGTGCGCGAGCTGGAGAACTGCGTGCAGCGGA
CGGCGACCCTGGCTGCGGGATCGGCGATCGGCCAGCACGATTTCGCCTGCAGCCGCAATGAATGCATGTCGGCGATCCTG
TGGAAGGGCAACACCGTGACCCCGCCGCCGCCGCGCGGCCAGCCGATGGTGCCGCTGCCGATCCTGCCGCGGACATCAGC
ACCGATCGAGACTCCAGTCGATCTGCCTGCGGTTGAGGCCGACGATGGTGAGTACTCCGAGGCGATGGATGGCGGCGCTG
TCCCCGAGCGCGAGCGCATCGTCCAGGCGATGGAGCGCTCCGGCTGGGTCCAGGCCAAGGCGGCGCGGCTGCTGGGCCTG
ACACCGCGCCAGATCGGCTACGCGCTGAAGAAGTACAACATCGAGGTCAAGCGGTTCTGA

Upstream 100 bases:

>100_bases
GTTAGGCACGACAATCATCTGCAATTAAGGAAGGATGTTAGAACGCCTACAATCGCGGGTGTTCTCGTCAAGAGCGTCCG
TGACCATCAGAGAAGCGACC

Downstream 100 bases:

>100_bases
GCGGATTGATTTTGCTCGGATCAAAAGGCGCGCGGATGTGCGCCTTTTCTGTTATGGGTGCATGCATCGTCATCGATCCG
ATGGTCGTGAAATCCGAGCT

Product: Mo/Fe nitrogenase specific transcriptional regulator, NifA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MVQTETRQVEAQDIRSHAAPIPLSEIALTGIFEISKILAAPTRLEITLANVVNLLQSFMQMRHGVVSLLADDGIPDLAVG
AGWSEGSDQRYRERLPQKAIDQIVATAVPLIAENVADHSAFSAADAATLGAGADTRVSFLGVPIRIDQRVVGTLTIDRVW
DGRAVFRLDSDVRFLTMVANLIGQTIKLHRVVARDRERLMAESHRLQKELMELKPAPAKGKKVLVDGIVGDSPALRALLD
KINVVAKSNAPVLLRGESGTGKELVAKAIHELSNRAKRPFIKINCAALPETVLESELFGHEKGAFTGAINARKGRFELAD
KGTLFLDEIGEISPAFQAKLLRVLQEQEFERVGGNQTIKVDVRIVTATNRNLEEAVARNEFRADLYYRISVVPIKLPPLR
DRRSDIPQLAREFLRRFNCENERDLTFDVSAIEVLMHCGFPGNVRELENCVQRTATLAAGSAIGQHDFACSRNECMSAIL
WKGNTVTPPPPRGQPMVPLPILPRTSAPIETPVDLPAVEADDGEYSEAMDGGAVPERERIVQAMERSGWVQAKAARLLGL
TPRQIGYALKKYNIEVKRF

Sequences:

>Translated_579_residues
MVQTETRQVEAQDIRSHAAPIPLSEIALTGIFEISKILAAPTRLEITLANVVNLLQSFMQMRHGVVSLLADDGIPDLAVG
AGWSEGSDQRYRERLPQKAIDQIVATAVPLIAENVADHSAFSAADAATLGAGADTRVSFLGVPIRIDQRVVGTLTIDRVW
DGRAVFRLDSDVRFLTMVANLIGQTIKLHRVVARDRERLMAESHRLQKELMELKPAPAKGKKVLVDGIVGDSPALRALLD
KINVVAKSNAPVLLRGESGTGKELVAKAIHELSNRAKRPFIKINCAALPETVLESELFGHEKGAFTGAINARKGRFELAD
KGTLFLDEIGEISPAFQAKLLRVLQEQEFERVGGNQTIKVDVRIVTATNRNLEEAVARNEFRADLYYRISVVPIKLPPLR
DRRSDIPQLAREFLRRFNCENERDLTFDVSAIEVLMHCGFPGNVRELENCVQRTATLAAGSAIGQHDFACSRNECMSAIL
WKGNTVTPPPPRGQPMVPLPILPRTSAPIETPVDLPAVEADDGEYSEAMDGGAVPERERIVQAMERSGWVQAKAARLLGL
TPRQIGYALKKYNIEVKRF
>Mature_579_residues
MVQTETRQVEAQDIRSHAAPIPLSEIALTGIFEISKILAAPTRLEITLANVVNLLQSFMQMRHGVVSLLADDGIPDLAVG
AGWSEGSDQRYRERLPQKAIDQIVATAVPLIAENVADHSAFSAADAATLGAGADTRVSFLGVPIRIDQRVVGTLTIDRVW
DGRAVFRLDSDVRFLTMVANLIGQTIKLHRVVARDRERLMAESHRLQKELMELKPAPAKGKKVLVDGIVGDSPALRALLD
KINVVAKSNAPVLLRGESGTGKELVAKAIHELSNRAKRPFIKINCAALPETVLESELFGHEKGAFTGAINARKGRFELAD
KGTLFLDEIGEISPAFQAKLLRVLQEQEFERVGGNQTIKVDVRIVTATNRNLEEAVARNEFRADLYYRISVVPIKLPPLR
DRRSDIPQLAREFLRRFNCENERDLTFDVSAIEVLMHCGFPGNVRELENCVQRTATLAAGSAIGQHDFACSRNECMSAIL
WKGNTVTPPPPRGQPMVPLPILPRTSAPIETPVDLPAVEADDGEYSEAMDGGAVPERERIVQAMERSGWVQAKAARLLGL
TPRQIGYALKKYNIEVKRF

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=349, Percent_Identity=44.4126074498567, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1790437, Length=370, Percent_Identity=43.7837837837838, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI87082117, Length=272, Percent_Identity=49.2647058823529, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1789087, Length=295, Percent_Identity=47.7966101694915, Blast_Score=259, Evalue=5e-70,
Organism=Escherichia coli, GI87082152, Length=240, Percent_Identity=53.75, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1790299, Length=357, Percent_Identity=41.1764705882353, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1788905, Length=253, Percent_Identity=47.8260869565217, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI87081872, Length=351, Percent_Identity=38.7464387464387, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1789233, Length=233, Percent_Identity=48.068669527897, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1786524, Length=251, Percent_Identity=45.4183266932271, Blast_Score=208, Evalue=8e-55,
Organism=Escherichia coli, GI1787583, Length=243, Percent_Identity=43.2098765432099, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789828, Length=256, Percent_Identity=39.0625, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI87081858, Length=225, Percent_Identity=36, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 63702; Mature: 63702

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQTETRQVEAQDIRSHAAPIPLSEIALTGIFEISKILAAPTRLEITLANVVNLLQSFMQ
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
MRHGVVSLLADDGIPDLAVGAGWSEGSDQRYRERLPQKAIDQIVATAVPLIAENVADHSA
HHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
FSAADAATLGAGADTRVSFLGVPIRIDQRVVGTLTIDRVWDGRAVFRLDSDVRFLTMVAN
HHHHHHHHCCCCCCCEEEEEECCEEECCEEEEEEEEEEEECCEEEEEECCCHHHHHHHHH
LIGQTIKLHRVVARDRERLMAESHRLQKELMELKPAPAKGKKVLVDGIVGDSPALRALLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHH
KINVVAKSNAPVLLRGESGTGKELVAKAIHELSNRAKRPFIKINCAALPETVLESELFGH
HHHHEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHCCHHHHHHHHHCC
EKGAFTGAINARKGRFELADKGTLFLDEIGEISPAFQAKLLRVLQEQEFERVGGNQTIKV
CCCCEEEECCCCCCCEEECCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
DVRIVTATNRNLEEAVARNEFRADLYYRISVVPIKLPPLRDRRSDIPQLAREFLRRFNCE
EEEEEEECCCCHHHHHHHHHHCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
NERDLTFDVSAIEVLMHCGFPGNVRELENCVQRTATLAAGSAIGQHDFACSRNECMSAIL
CCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
WKGNTVTPPPPRGQPMVPLPILPRTSAPIETPVDLPAVEADDGEYSEAMDGGAVPERERI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHCCCCCCCHHHHH
VQAMERSGWVQAKAARLLGLTPRQIGYALKKYNIEVKRF
HHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCEEEECC
>Mature Secondary Structure
MVQTETRQVEAQDIRSHAAPIPLSEIALTGIFEISKILAAPTRLEITLANVVNLLQSFMQ
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
MRHGVVSLLADDGIPDLAVGAGWSEGSDQRYRERLPQKAIDQIVATAVPLIAENVADHSA
HHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
FSAADAATLGAGADTRVSFLGVPIRIDQRVVGTLTIDRVWDGRAVFRLDSDVRFLTMVAN
HHHHHHHHCCCCCCCEEEEEECCEEECCEEEEEEEEEEEECCEEEEEECCCHHHHHHHHH
LIGQTIKLHRVVARDRERLMAESHRLQKELMELKPAPAKGKKVLVDGIVGDSPALRALLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHH
KINVVAKSNAPVLLRGESGTGKELVAKAIHELSNRAKRPFIKINCAALPETVLESELFGH
HHHHEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHCCHHHHHHHHHCC
EKGAFTGAINARKGRFELADKGTLFLDEIGEISPAFQAKLLRVLQEQEFERVGGNQTIKV
CCCCEEEECCCCCCCEEECCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
DVRIVTATNRNLEEAVARNEFRADLYYRISVVPIKLPPLRDRRSDIPQLAREFLRRFNCE
EEEEEEECCCCHHHHHHHHHHCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
NERDLTFDVSAIEVLMHCGFPGNVRELENCVQRTATLAAGSAIGQHDFACSRNECMSAIL
CCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
WKGNTVTPPPPRGQPMVPLPILPRTSAPIETPVDLPAVEADDGEYSEAMDGGAVPERERI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHCCCCCCCHHHHH
VQAMERSGWVQAKAARLLGLTPRQIGYALKKYNIEVKRF
HHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3313281; 12597275; 3357773; 2792368 [H]