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The map label for this gene is nifS [H]

Identifier: 146342269

GI number: 146342269

Start: 5613659

End: 5614873

Strand: Reverse

Name: nifS [H]

Synonym: BRADO5423

Alternate gene names: 146342269

Gene position: 5614873-5613659 (Counterclockwise)

Preceding gene: 146342270

Following gene: 146342268

Centisome position: 75.3

GC content: 68.48

Gene sequence:

>1215_bases
GTGCGTCCGGTGTACCTCGACAACAACGCCACGACGCGCGTCGACCCCGAGGTCGTCGCGGCGATGTTGCCGTTCTTCAC
GGACCAGTTCGGCAATGCCTCGTCCTCGCATGCCTTCGGCAGCGAGGTCGCGGGCGCCGTCCGCCAGGCGCGCAAGAGCC
TGCAGGCGCTGCTCGGCGCCGCGTTCGACCACGAGATCGTCTACACCTCGGGCGGGACGGAATCGGACAACACCGCGATC
CTCTCGGCGCTCGAGACTCAAGACGGGCGCGACGAGATCGTCACCACCGCGGTCGAGCATCCCGCGATCCTGACGCTGGT
CGAGCATCTCCGCGACAAGCGCGGCGTCACAGTCCACCTGATCGGCGTCGATGCCGCCGGGCGGCTCGATGTCGACGCCT
ACAAGCGCGCGCTTGGGCCGCGCACCGCGATCGCGTCCGTGATGTGGGCCAACAACGAGACCGGCACGCTGAACCCCATT
CCGGAGCTGGCCAAGCTCGCGCATGATGCCGGCGCGCTGTTCCATACCGATGCCGTGCAGGCCGTCGGCAAGATCGCCAT
TGACCTGAAAGCCACTGCCGTCGACATGCTGTCGCTGTCCGGACACAAGCTGCACGGGCCGAAGGGCATCGGTGCGCTGT
ATGTCAGGAAGGGCACGCCGTTCGCGCCGCTGGTGCGCGGTGGCCCGCAGGAGCGCCGCCGGCGTGGCGGCACCGAGAAT
GTTCCCGCCATCATCGGCCTTGGCAAGGCGGCCGAACTCGCCGCCAAGCATCTCGCCGACGAGCAGGGCCGAGTCCGCGC
GCTGCGCGACCGGCTGGAGCAGGGAATCACCCAGGGCGTCGGCCATTGCGCCATGCTCGGCGATGTTGCGCAGCGACTCC
CGAACACCGCCAATATCGCGTTCCAGCATCTCGAAGGCGAGCCGATCGTGATGCGGCTGAACCAGGCCGGTATCGCCGCC
TCGCTCGGCTCCGCCTGCGCCTCCGGCTCGATGGAGCCGTCGCACGTGCTCAGGGCGATGAACGTACCGCCGGAGCTGCT
GCGCGGCGCGGTGCGCTTCTCGTTGTCGCGCGACACCACCGACGACGATATCGATGGTGTGCTGGCCGTGCTGCCCGACA
TCGTCGGCCGTCTCCGCGAGACCTCGCCGTCCTGGCTGCAACATCATCCGTCAAACGCGGCGGACGCCCTTCGTCCCACG
GAGCTTACGCAATGA

Upstream 100 bases:

>100_bases
CCTGGAAGGCATCCAGGCCCGCATGATCGAGAAGCTCGGCGAGTTCGTCCGTCTGGTTCCGGTGGCCGGCGCTGCGAAGG
CGCGGCATTAAGGAGCGGCC

Downstream 100 bases:

>100_bases
AAGTCATGATCCGCCGCTCGCCGGAAACCGGCCTCTCCATCTACGTCCCCAAGAAGGACCTGGAGGAGCCGATCGTCGAG
ACCGAGCACGAGACCTTGTG

Product: cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MRPVYLDNNATTRVDPEVVAAMLPFFTDQFGNASSSHAFGSEVAGAVRQARKSLQALLGAAFDHEIVYTSGGTESDNTAI
LSALETQDGRDEIVTTAVEHPAILTLVEHLRDKRGVTVHLIGVDAAGRLDVDAYKRALGPRTAIASVMWANNETGTLNPI
PELAKLAHDAGALFHTDAVQAVGKIAIDLKATAVDMLSLSGHKLHGPKGIGALYVRKGTPFAPLVRGGPQERRRRGGTEN
VPAIIGLGKAAELAAKHLADEQGRVRALRDRLEQGITQGVGHCAMLGDVAQRLPNTANIAFQHLEGEPIVMRLNQAGIAA
SLGSACASGSMEPSHVLRAMNVPPELLRGAVRFSLSRDTTDDDIDGVLAVLPDIVGRLRETSPSWLQHHPSNAADALRPT
ELTQ

Sequences:

>Translated_404_residues
MRPVYLDNNATTRVDPEVVAAMLPFFTDQFGNASSSHAFGSEVAGAVRQARKSLQALLGAAFDHEIVYTSGGTESDNTAI
LSALETQDGRDEIVTTAVEHPAILTLVEHLRDKRGVTVHLIGVDAAGRLDVDAYKRALGPRTAIASVMWANNETGTLNPI
PELAKLAHDAGALFHTDAVQAVGKIAIDLKATAVDMLSLSGHKLHGPKGIGALYVRKGTPFAPLVRGGPQERRRRGGTEN
VPAIIGLGKAAELAAKHLADEQGRVRALRDRLEQGITQGVGHCAMLGDVAQRLPNTANIAFQHLEGEPIVMRLNQAGIAA
SLGSACASGSMEPSHVLRAMNVPPELLRGAVRFSLSRDTTDDDIDGVLAVLPDIVGRLRETSPSWLQHHPSNAADALRPT
ELTQ
>Mature_404_residues
MRPVYLDNNATTRVDPEVVAAMLPFFTDQFGNASSSHAFGSEVAGAVRQARKSLQALLGAAFDHEIVYTSGGTESDNTAI
LSALETQDGRDEIVTTAVEHPAILTLVEHLRDKRGVTVHLIGVDAAGRLDVDAYKRALGPRTAIASVMWANNETGTLNPI
PELAKLAHDAGALFHTDAVQAVGKIAIDLKATAVDMLSLSGHKLHGPKGIGALYVRKGTPFAPLVRGGPQERRRRGGTEN
VPAIIGLGKAAELAAKHLADEQGRVRALRDRLEQGITQGVGHCAMLGDVAQRLPNTANIAFQHLEGEPIVMRLNQAGIAA
SLGSACASGSMEPSHVLRAMNVPPELLRGAVRFSLSRDTTDDDIDGVLAVLPDIVGRLRETSPSWLQHHPSNAADALRPT
ELTQ

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=393, Percent_Identity=39.1857506361323, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI156713448, Length=418, Percent_Identity=36.1244019138756, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI48994898, Length=393, Percent_Identity=41.7302798982188, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1789175, Length=386, Percent_Identity=27.4611398963731, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787970, Length=271, Percent_Identity=27.3062730627306, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI25143064, Length=393, Percent_Identity=39.1857506361323, Blast_Score=280, Evalue=7e-76,
Organism=Caenorhabditis elegans, GI17533177, Length=313, Percent_Identity=29.7124600638978, Blast_Score=151, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6319831, Length=388, Percent_Identity=39.1752577319588, Blast_Score=273, Evalue=4e-74,
Organism=Drosophila melanogaster, GI20129463, Length=388, Percent_Identity=39.4329896907216, Blast_Score=271, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42878; Mature: 42878

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPVYLDNNATTRVDPEVVAAMLPFFTDQFGNASSSHAFGSEVAGAVRQARKSLQALLGA
CCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AFDHEIVYTSGGTESDNTAILSALETQDGRDEIVTTAVEHPAILTLVEHLRDKRGVTVHL
HCCCEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEE
IGVDAAGRLDVDAYKRALGPRTAIASVMWANNETGTLNPIPELAKLAHDAGALFHTDAVQ
EEECCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
AVGKIAIDLKATAVDMLSLSGHKLHGPKGIGALYVRKGTPFAPLVRGGPQERRRRGGTEN
HHHHHHEEEHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHCCCHHHHHHCCCCCC
VPAIIGLGKAAELAAKHLADEQGRVRALRDRLEQGITQGVGHCAMLGDVAQRLPNTANIA
CCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHE
FQHLEGEPIVMRLNQAGIAASLGSACASGSMEPSHVLRAMNVPPELLRGAVRFSLSRDTT
EEECCCCCEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHEECCCCC
DDDIDGVLAVLPDIVGRLRETSPSWLQHHPSNAADALRPTELTQ
CHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHCCCCCCCCC
>Mature Secondary Structure
MRPVYLDNNATTRVDPEVVAAMLPFFTDQFGNASSSHAFGSEVAGAVRQARKSLQALLGA
CCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AFDHEIVYTSGGTESDNTAILSALETQDGRDEIVTTAVEHPAILTLVEHLRDKRGVTVHL
HCCCEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEE
IGVDAAGRLDVDAYKRALGPRTAIASVMWANNETGTLNPIPELAKLAHDAGALFHTDAVQ
EEECCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
AVGKIAIDLKATAVDMLSLSGHKLHGPKGIGALYVRKGTPFAPLVRGGPQERRRRGGTEN
HHHHHHEEEHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHCCCHHHHHHCCCCCC
VPAIIGLGKAAELAAKHLADEQGRVRALRDRLEQGITQGVGHCAMLGDVAQRLPNTANIA
CCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHE
FQHLEGEPIVMRLNQAGIAASLGSACASGSMEPSHVLRAMNVPPELLRGAVRFSLSRDTT
EEECCCCCEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHEECCCCC
DDDIDGVLAVLPDIVGRLRETSPSWLQHHPSNAADALRPTELTQ
CHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11092875 [H]