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The map label for this gene is yoaD [H]

Identifier: 146341689

GI number: 146341689

Start: 4990729

End: 4991769

Strand: Reverse

Name: yoaD [H]

Synonym: BRADO4794

Alternate gene names: 146341689

Gene position: 4991769-4990729 (Counterclockwise)

Preceding gene: 146341690

Following gene: 146341688

Centisome position: 66.94

GC content: 67.63

Gene sequence:

>1041_bases
ATGTCTAGCTCCATCGCCATCATCGGCGACCGTTTCATGCTGCCCTCGGTGTTCGCCGAACGCATCACGGCGATCTGCGG
CGATCATCTCGACATCCGCATGCTCGAGCAGCCCTGGCCGGACGAGCCGATGGAGCATGGCTATGCCGGCTCCAAGCTCG
ACGGTCTCAAGGAGTTCATGGGCGATCCCGATGAGCTCGCCGATTTCATCGGCGAGGCACCGCTGCTGGTGACCCATCTG
GCGCCGATTTCGCGCGCGATGCTGCAGCGGCTGCCGCGGCTGAAATTCATCGCGGTGTCGCGCGGCGGCCCGGTGAATGT
CGACATGCAGGCGGCGCGTGATCACGGTGTGCTCGTCGTCAACACACCCGGTCGTAACGCCAGCGCGGTCGCCGAATTCA
CCATCGGCGCGATGCTCGCCGAGACTCGGCTGATCCGCAGCGGCCATGAATCGATGCGGGGCGGGGAGTGGCGCGGCGAT
CTCTACCGTGCCGATCGCACGGGCCGCGAGCTCGGCGAGATGACCGTCGGCATCGTCGGCTATGGCGCGATCGGCACCCG
CGTGGTGAAGTTGCTGAAAGCGTTCGGCTGCAGGATCCTGGTGACGGACCCCTACGTCCAACTCACCGCCCAGGACCGCA
ATGACGGCGTCGAGCATGTCGCGCTGGCCGAGCTGCTGTCGCGCGCCGACGTCATCAGCCTGCATGCCAGGGTGACGTCG
GAGACCACCGGCTTCATCGATCGCGAGGCGCTGGCGCGGATCAAGCCGGGCGCGATCCTGATCAACACGGCGCGCGGGCC
GCTGGTCGACTACAAGGCGCTGTTCGAGGTGCTGAGCTCGGGGCGGCTCGCCGGTGCGATGCTCGACACCTTCGCGATCG
AGCCGGTGCCGCCGGACTGGCCGCTGCTGCAACTGCCCAACGTGACGCTGACGCCGCACATCGCCGGCGCCTCCGTCCGA
ACCGTCACCATCGCCGCCGACCAGGCGGCCGAAGAGGTTCGCCGCTATCTCGCCGGCGAGCCGCCGCTCAATCCCTGCTG
A

Upstream 100 bases:

>100_bases
GCGGAGCGCTATGCGCGGCTGTTCCCGGCCTATGTCGAGGCACGCCTGGCGGCGCGACCCGTCTGGAAGCAGCTTGCGGC
GCAGCGCGCGGGAGCCAACC

Downstream 100 bases:

>100_bases
CGAAAGGTTTTCCAAATGAATCGCGAGGAACGACAGCTCCGCGAGACCATCATCGCCAAGTGCCGGTGGATGAACGCCTC
GGGTCTGAATCAGGGCACGT

Product: putative D-3-phosphoglycerate dehydrogenase

Products: 3-phosphohydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSSSIAIIGDRFMLPSVFAERITAICGDHLDIRMLEQPWPDEPMEHGYAGSKLDGLKEFMGDPDELADFIGEAPLLVTHL
APISRAMLQRLPRLKFIAVSRGGPVNVDMQAARDHGVLVVNTPGRNASAVAEFTIGAMLAETRLIRSGHESMRGGEWRGD
LYRADRTGRELGEMTVGIVGYGAIGTRVVKLLKAFGCRILVTDPYVQLTAQDRNDGVEHVALAELLSRADVISLHARVTS
ETTGFIDREALARIKPGAILINTARGPLVDYKALFEVLSSGRLAGAMLDTFAIEPVPPDWPLLQLPNVTLTPHIAGASVR
TVTIAADQAAEEVRRYLAGEPPLNPC

Sequences:

>Translated_346_residues
MSSSIAIIGDRFMLPSVFAERITAICGDHLDIRMLEQPWPDEPMEHGYAGSKLDGLKEFMGDPDELADFIGEAPLLVTHL
APISRAMLQRLPRLKFIAVSRGGPVNVDMQAARDHGVLVVNTPGRNASAVAEFTIGAMLAETRLIRSGHESMRGGEWRGD
LYRADRTGRELGEMTVGIVGYGAIGTRVVKLLKAFGCRILVTDPYVQLTAQDRNDGVEHVALAELLSRADVISLHARVTS
ETTGFIDREALARIKPGAILINTARGPLVDYKALFEVLSSGRLAGAMLDTFAIEPVPPDWPLLQLPNVTLTPHIAGASVR
TVTIAADQAAEEVRRYLAGEPPLNPC
>Mature_345_residues
SSSIAIIGDRFMLPSVFAERITAICGDHLDIRMLEQPWPDEPMEHGYAGSKLDGLKEFMGDPDELADFIGEAPLLVTHLA
PISRAMLQRLPRLKFIAVSRGGPVNVDMQAARDHGVLVVNTPGRNASAVAEFTIGAMLAETRLIRSGHESMRGGEWRGDL
YRADRTGRELGEMTVGIVGYGAIGTRVVKLLKAFGCRILVTDPYVQLTAQDRNDGVEHVALAELLSRADVISLHARVTSE
TTGFIDREALARIKPGAILINTARGPLVDYKALFEVLSSGRLAGAMLDTFAIEPVPPDWPLLQLPNVTLTPHIAGASVRT
VTIAADQAAEEVRRYLAGEPPLNPC

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=269, Percent_Identity=33.8289962825279, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI61743967, Length=273, Percent_Identity=32.967032967033, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI4557497, Length=273, Percent_Identity=32.967032967033, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI145580578, Length=273, Percent_Identity=32.967032967033, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4557499, Length=273, Percent_Identity=32.967032967033, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI145580575, Length=273, Percent_Identity=32.967032967033, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI6912396, Length=253, Percent_Identity=31.2252964426877, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1787645, Length=213, Percent_Identity=34.7417840375587, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789279, Length=220, Percent_Identity=34.5454545454545, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87082289, Length=259, Percent_Identity=30.5019305019305, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081824, Length=150, Percent_Identity=38, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788660, Length=294, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=247, Percent_Identity=32.3886639676113, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25147481, Length=275, Percent_Identity=30.1818181818182, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6322116, Length=267, Percent_Identity=29.2134831460674, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=258, Percent_Identity=29.0697674418605, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=267, Percent_Identity=28.8389513108614, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6324055, Length=221, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324980, Length=195, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28574286, Length=257, Percent_Identity=32.295719844358, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19921140, Length=241, Percent_Identity=32.3651452282158, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28574284, Length=256, Percent_Identity=30.46875, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585514, Length=256, Percent_Identity=30.46875, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28574282, Length=256, Percent_Identity=30.46875, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45552429, Length=256, Percent_Identity=30.46875, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45551003, Length=256, Percent_Identity=30.46875, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24646446, Length=276, Percent_Identity=31.5217391304348, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24646448, Length=276, Percent_Identity=31.5217391304348, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24646452, Length=276, Percent_Identity=31.5217391304348, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24646450, Length=276, Percent_Identity=31.5217391304348, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI62472511, Length=276, Percent_Identity=31.5217391304348, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585516, Length=271, Percent_Identity=26.5682656826568, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI28571528, Length=249, Percent_Identity=31.7269076305221, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR015508
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.95

Molecular weight: Translated: 37460; Mature: 37328

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSIAIIGDRFMLPSVFAERITAICGDHLDIRMLEQPWPDEPMEHGYAGSKLDGLKEFM
CCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHCCCCCCHHHHHHHHC
GDPDELADFIGEAPLLVTHLAPISRAMLQRLPRLKFIAVSRGGPVNVDMQAARDHGVLVV
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEHHHCCCCCEEEE
NTPGRNASAVAEFTIGAMLAETRLIRSGHESMRGGEWRGDLYRADRTGRELGEMTVGIVG
ECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEE
YGAIGTRVVKLLKAFGCRILVTDPYVQLTAQDRNDGVEHVALAELLSRADVISLHARVTS
CCHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEHEEECC
ETTGFIDREALARIKPGAILINTARGPLVDYKALFEVLSSGRLAGAMLDTFAIEPVPPDW
CCCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
PLLQLPNVTLTPHIAGASVRTVTIAADQAAEEVRRYLAGEPPLNPC
CCEECCCEEECCCCCCCCEEEEEEECHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSSIAIIGDRFMLPSVFAERITAICGDHLDIRMLEQPWPDEPMEHGYAGSKLDGLKEFM
CCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHCCCCCCHHHHHHHHC
GDPDELADFIGEAPLLVTHLAPISRAMLQRLPRLKFIAVSRGGPVNVDMQAARDHGVLVV
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEHHHCCCCCEEEE
NTPGRNASAVAEFTIGAMLAETRLIRSGHESMRGGEWRGDLYRADRTGRELGEMTVGIVG
ECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEE
YGAIGTRVVKLLKAFGCRILVTDPYVQLTAQDRNDGVEHVALAELLSRADVISLHARVTS
CCHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEHEEECC
ETTGFIDREALARIKPGAILINTARGPLVDYKALFEVLSSGRLAGAMLDTFAIEPVPPDW
CCCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
PLLQLPNVTLTPHIAGASVRTVTIAADQAAEEVRRYLAGEPPLNPC
CCEECCCEEECCCCCCCCEEEEEEECHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-phosphoglycerate; NAD+

Specific reaction: 3-phosphoglycerate + NAD+ = 3-phosphohydroxypyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]