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The map label for this gene is gnl [H]

Identifier: 146341664

GI number: 146341664

Start: 4961416

End: 4963005

Strand: Reverse

Name: gnl [H]

Synonym: BRADO4769

Alternate gene names: 146341664

Gene position: 4963005-4961416 (Counterclockwise)

Preceding gene: 146341665

Following gene: 146341663

Centisome position: 66.56

GC content: 66.79

Gene sequence:

>1590_bases
ATGACCCACGTCACCTTGCGCCCCGAGTTTGAGAGCCTGATCGATCCCTACGCGCCGGTGGCGCAGGTCGCGACCGGCTT
CGAGTTCACGGAGGGGCCGATCTGGCATCCCGCCGAGCAGTTCCTGCTGTTCTCCGACATGCCGGCCGATGTCAGGCGAC
GCTGGGACGCCAGGCGCGGCGTCGTCGAGATGCGGCGGCCTTCGAACAAGTGCAACGGCATGACCTACGATGCCGAGCTC
AATCTCATCGTCTGCGAGCACGCGACGTCATCGCTGGTGCGCGAGCGCCCCGACGGGCGCCGCGAGGTCCTCGCTTCGCA
CTATGAGAACCAGGAGCTCAACAGCCCCAACGATGTCTGCGTTCATTCCAGCGGCGCGATGTATTTTTCCGATCCCTGGT
ATGGCCGCATGCCGGTCTATGGCGTCGAGCGGCCGCGCCAGCTCGGCTTCCAGGGCGTCTTTCGTGTGCCGCCTGGCGGC
GGTGCGCCGCAGCTGCTCGTCGATCGCCATCTGTTCGATCAGCCCAACGGGCTGTGTTTCTCGCCTGACGAGAAGCTGCT
CTATGTCAACGACACGGTGCAGGCGCTGATCCGTGTGTTCGATGTCGGCAGCGACGGCGCGCTGTCGCACGGTCGCGTCT
TCGCCAGCGGCATCCGCTCTGAACTCGAGCCGGGCGTGCCCGACGGCATGAAATGCGATCAGCGCGGCAATGTCTGGGTC
ACCGCGCCTGGCGGGGCCTGGGTCTACGCGCCGACAGGCGAGCTGCTCGGCAAGGTGCGCGTGCCGGAGCTGGTGGCTAA
TCTGGCCTGGGGCGGTGATGATTTCCGGACGCTGTATCTCACGGCGACGCATTCGGTCTACGCGATCAAGACCAAGGTCG
GTCCACGCCACGAGCCGTATATGAGCGCGCGGCGTACGGCCGAGACGATATCGTCGGCATCGGTCGCGTCGGCGACTGCC
GCGCCTCCGCTGCTCGCCTCCGGCGACATGCAGCTCGATCCGCGTCGCTGCGCCATGATCATCCAGGACCTGCAGAACGA
CGTCATCATGGAGGGCGGCGCCTTTGCCGACTCCGGCGCGCCAGGACATGCCAAGCAGCAGCGCGTCGTCGACAACGTCC
GCCGGCTCGCCGAGGTGGCGCGCGCGCGCGGCGTCGTCATCATCCATGTGTGGTTCGTGGTCGAGCCGGGCGCGCCCGGC
GTGACCCTGAACGCGCCGCTGTTCGAAGGGCTGGTCGACAGCAGGGCCATGGTGAGAGGCAGCTGGGGCGCGGCGCCCGT
CGCCGGCCTCGAACCGCGCAGTGGCGACTTCGTGGTCGAGAAGATGCGGATGAGCGCGTGGGAGGGGACACGGCTCGAGA
CCATCCTGAAGGCGACCGGGCGCGACATCATCATCAACACCGGTGCCTGGACCAACATGTCGGTCGAGCACACCGCGCGC
ACTGGCGCCGACAAGGGCTACTTCATGGTGGTGCCGGAGGACTGCTGCTCGACCATGAATGCGGACTGGCACAATGCGTC
GGTCAACTTCGCCTTGCAGAACGTGTCGGTCGTCACCCGGGCCGATGACGTCATCAAGGCGCTGGGGTAG

Upstream 100 bases:

>100_bases
CGAGGGCGGAGTGGTGACGGGGCGGCTGCATGACCAGGTGCCGGGCCCGGTCGTGCAGTCGCTGCCGCAGCGGCATTGGT
TTCCGGCGTGAGGACGCGTC

Downstream 100 bases:

>100_bases
GGGATGCCCAAGCTGCTCCATCTCGCCGCCTCGCCGCGCGCGGATTCGGAATCCGGCGCGGCTTCACGCGTCTTTCTCGA
CCGCTTCAGGCAGGCCCGGC

Product: putative gluconolactonase

Products: NA

Alternate protein names: D-glucono-delta-lactone lactonohydrolase [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MTHVTLRPEFESLIDPYAPVAQVATGFEFTEGPIWHPAEQFLLFSDMPADVRRRWDARRGVVEMRRPSNKCNGMTYDAEL
NLIVCEHATSSLVRERPDGRREVLASHYENQELNSPNDVCVHSSGAMYFSDPWYGRMPVYGVERPRQLGFQGVFRVPPGG
GAPQLLVDRHLFDQPNGLCFSPDEKLLYVNDTVQALIRVFDVGSDGALSHGRVFASGIRSELEPGVPDGMKCDQRGNVWV
TAPGGAWVYAPTGELLGKVRVPELVANLAWGGDDFRTLYLTATHSVYAIKTKVGPRHEPYMSARRTAETISSASVASATA
APPLLASGDMQLDPRRCAMIIQDLQNDVIMEGGAFADSGAPGHAKQQRVVDNVRRLAEVARARGVVIIHVWFVVEPGAPG
VTLNAPLFEGLVDSRAMVRGSWGAAPVAGLEPRSGDFVVEKMRMSAWEGTRLETILKATGRDIIINTGAWTNMSVEHTAR
TGADKGYFMVVPEDCCSTMNADWHNASVNFALQNVSVVTRADDVIKALG

Sequences:

>Translated_529_residues
MTHVTLRPEFESLIDPYAPVAQVATGFEFTEGPIWHPAEQFLLFSDMPADVRRRWDARRGVVEMRRPSNKCNGMTYDAEL
NLIVCEHATSSLVRERPDGRREVLASHYENQELNSPNDVCVHSSGAMYFSDPWYGRMPVYGVERPRQLGFQGVFRVPPGG
GAPQLLVDRHLFDQPNGLCFSPDEKLLYVNDTVQALIRVFDVGSDGALSHGRVFASGIRSELEPGVPDGMKCDQRGNVWV
TAPGGAWVYAPTGELLGKVRVPELVANLAWGGDDFRTLYLTATHSVYAIKTKVGPRHEPYMSARRTAETISSASVASATA
APPLLASGDMQLDPRRCAMIIQDLQNDVIMEGGAFADSGAPGHAKQQRVVDNVRRLAEVARARGVVIIHVWFVVEPGAPG
VTLNAPLFEGLVDSRAMVRGSWGAAPVAGLEPRSGDFVVEKMRMSAWEGTRLETILKATGRDIIINTGAWTNMSVEHTAR
TGADKGYFMVVPEDCCSTMNADWHNASVNFALQNVSVVTRADDVIKALG
>Mature_528_residues
THVTLRPEFESLIDPYAPVAQVATGFEFTEGPIWHPAEQFLLFSDMPADVRRRWDARRGVVEMRRPSNKCNGMTYDAELN
LIVCEHATSSLVRERPDGRREVLASHYENQELNSPNDVCVHSSGAMYFSDPWYGRMPVYGVERPRQLGFQGVFRVPPGGG
APQLLVDRHLFDQPNGLCFSPDEKLLYVNDTVQALIRVFDVGSDGALSHGRVFASGIRSELEPGVPDGMKCDQRGNVWVT
APGGAWVYAPTGELLGKVRVPELVANLAWGGDDFRTLYLTATHSVYAIKTKVGPRHEPYMSARRTAETISSASVASATAA
PPLLASGDMQLDPRRCAMIIQDLQNDVIMEGGAFADSGAPGHAKQQRVVDNVRRLAEVARARGVVIIHVWFVVEPGAPGV
TLNAPLFEGLVDSRAMVRGSWGAAPVAGLEPRSGDFVVEKMRMSAWEGTRLETILKATGRDIIINTGAWTNMSVEHTART
GADKGYFMVVPEDCCSTMNADWHNASVNFALQNVSVVTRADDVIKALG

Specific function: Hydrolyzes the gluconolactone formed by glucose-fructose oxidoreductase, and that formed in aerobic conditions by the glucose dehydrogenase present [H]

COG id: COG3386

COG function: function code G; Gluconolactonase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081820, Length=196, Percent_Identity=31.6326530612245, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87081992, Length=202, Percent_Identity=29.2079207920792, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011042
- InterPro:   IPR013658
- InterPro:   IPR005511
- InterPro:   IPR006311 [H]

Pfam domain/function: PF08450 SGL [H]

EC number: =3.1.1.17 [H]

Molecular weight: Translated: 57874; Mature: 57743

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVTLRPEFESLIDPYAPVAQVATGFEFTEGPIWHPAEQFLLFSDMPADVRRRWDARRG
CCEEEECCCHHHHCCCCCCHHHHHCCCCCCCCCCCCCHHHEEEECCCCHHHHHHHHHHCC
VVEMRRPSNKCNGMTYDAELNLIVCEHATSSLVRERPDGRREVLASHYENQELNSPNDVC
HHHHCCCCCCCCCEEEECEEEEEEECCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCEE
VHSSGAMYFSDPWYGRMPVYGVERPRQLGFQGVFRVPPGGGAPQLLVDRHLFDQPNGLCF
EECCCCEEECCCCCCCCCCCCCCCHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCEE
SPDEKLLYVNDTVQALIRVFDVGSDGALSHGRVFASGIRSELEPGVPDGMKCDQRGNVWV
CCCCCEEEECHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
TAPGGAWVYAPTGELLGKVRVPELVANLAWGGDDFRTLYLTATHSVYAIKTKVGPRHEPY
ECCCCEEEEECCHHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCCCCHH
MSARRTAETISSASVASATAAPPLLASGDMQLDPRRCAMIIQDLQNDVIMEGGAFADSGA
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHCEEECCCCCCCCCC
PGHAKQQRVVDNVRRLAEVARARGVVIIHVWFVVEPGAPGVTLNAPLFEGLVDSRAMVRG
CCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCEEECCHHHHHHHCCCHHEEC
SWGAAPVAGLEPRSGDFVVEKMRMSAWEGTRLETILKATGRDIIINTGAWTNMSVEHTAR
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCCCEEHHHHC
TGADKGYFMVVPEDCCSTMNADWHNASVNFALQNVSVVTRADDVIKALG
CCCCCCEEEEECHHHHCCCCCCCCCCEEEEEEECEEEEEHHHHHHHHCC
>Mature Secondary Structure 
THVTLRPEFESLIDPYAPVAQVATGFEFTEGPIWHPAEQFLLFSDMPADVRRRWDARRG
CEEEECCCHHHHCCCCCCHHHHHCCCCCCCCCCCCCHHHEEEECCCCHHHHHHHHHHCC
VVEMRRPSNKCNGMTYDAELNLIVCEHATSSLVRERPDGRREVLASHYENQELNSPNDVC
HHHHCCCCCCCCCEEEECEEEEEEECCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCEE
VHSSGAMYFSDPWYGRMPVYGVERPRQLGFQGVFRVPPGGGAPQLLVDRHLFDQPNGLCF
EECCCCEEECCCCCCCCCCCCCCCHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCEE
SPDEKLLYVNDTVQALIRVFDVGSDGALSHGRVFASGIRSELEPGVPDGMKCDQRGNVWV
CCCCCEEEECHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
TAPGGAWVYAPTGELLGKVRVPELVANLAWGGDDFRTLYLTATHSVYAIKTKVGPRHEPY
ECCCCEEEEECCHHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCCCCHH
MSARRTAETISSASVASATAAPPLLASGDMQLDPRRCAMIIQDLQNDVIMEGGAFADSGA
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHCEEECCCCCCCCCC
PGHAKQQRVVDNVRRLAEVARARGVVIIHVWFVVEPGAPGVTLNAPLFEGLVDSRAMVRG
CCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCEEECCHHHHHHHCCCHHEEC
SWGAAPVAGLEPRSGDFVVEKMRMSAWEGTRLETILKATGRDIIINTGAWTNMSVEHTAR
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCCCEEHHHHC
TGADKGYFMVVPEDCCSTMNADWHNASVNFALQNVSVVTRADDVIKALG
CCCCCCEEEEECHHHHCCCCCCCCCCEEEEEEECEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1482681 [H]