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The map label for this gene is azoR1 [H]

Identifier: 146341663

GI number: 146341663

Start: 4960786

End: 4961412

Strand: Reverse

Name: azoR1 [H]

Synonym: BRADO4768

Alternate gene names: 146341663

Gene position: 4961412-4960786 (Counterclockwise)

Preceding gene: 146341664

Following gene: 146341661

Centisome position: 66.54

GC content: 67.94

Gene sequence:

>627_bases
ATGCCCAAGCTGCTCCATCTCGCCGCCTCGCCGCGCGCGGATTCGGAATCCGGCGCGGCTTCACGCGTCTTTCTCGACCG
CTTCAGGCAGGCCCGGCCGGGTTTCGACATCGACGAGATGAACCTGTGGCGGGAGCATCTTCCCGAGTTCGACGGCGAGA
TCCTGCAGGCGAAATACGCGCGCATGGCCGGACGCGCGTTCACCGACGCTCAGCGCGCGGCCTTCGCCGTCGCCGAGCGC
ATGGCGGTGCGACTCGATCTCGCCGAGCGGGTGCTGATCTCGACGCCGATGTGGAATTTCGGCATTCCCTACAAGCTCAA
GCACTGGCTCGACATCATCAACCAGCCCGGGCTCACCTTTCGCTTCGATGCCGCCAGCGGCTATCTCCCGTTGTTGAAGG
ACCGGCCGACGCTGGTGATCCTTTCGAGCGGCGGTGATTTCTCGACCGGCATGAGCCGCGGCCGGACCGATCTCGCGACG
CCCTATCTGCGCGAGGCGCTGCGCTTCATGGGCCTGCGCGACGTCACGTTCGTCGCGATCGGTCCGACCAGCGGGCCGGC
CGCGCACACCGGCGCGGCGCGCGAGCGCGCGCATCGTCGGCTCGTGGAGATCGCCGCGCGGTTCTGA

Upstream 100 bases:

>100_bases
CATGAATGCGGACTGGCACAATGCGTCGGTCAACTTCGCCTTGCAGAACGTGTCGGTCGTCACCCGGGCCGATGACGTCA
TCAAGGCGCTGGGGTAGGGG

Downstream 100 bases:

>100_bases
CCGGAGCTTCGCACCGAGCTACGCGTCTGACAGCTCCGACCTGATCCAGTCGGCGAAGGCACGCACCGCCGAGCGCTGAT
GCTGCGTGCGTGGAACGACC

Product: putative [Acyl-carrier-protein] phosphodiesterase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 1; FMN-dependent NADH-azo compound oxidoreductase 1 [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MPKLLHLAASPRADSESGAASRVFLDRFRQARPGFDIDEMNLWREHLPEFDGEILQAKYARMAGRAFTDAQRAAFAVAER
MAVRLDLAERVLISTPMWNFGIPYKLKHWLDIINQPGLTFRFDAASGYLPLLKDRPTLVILSSGGDFSTGMSRGRTDLAT
PYLREALRFMGLRDVTFVAIGPTSGPAAHTGAARERAHRRLVEIAARF

Sequences:

>Translated_208_residues
MPKLLHLAASPRADSESGAASRVFLDRFRQARPGFDIDEMNLWREHLPEFDGEILQAKYARMAGRAFTDAQRAAFAVAER
MAVRLDLAERVLISTPMWNFGIPYKLKHWLDIINQPGLTFRFDAASGYLPLLKDRPTLVILSSGGDFSTGMSRGRTDLAT
PYLREALRFMGLRDVTFVAIGPTSGPAAHTGAARERAHRRLVEIAARF
>Mature_207_residues
PKLLHLAASPRADSESGAASRVFLDRFRQARPGFDIDEMNLWREHLPEFDGEILQAKYARMAGRAFTDAQRAAFAVAERM
AVRLDLAERVLISTPMWNFGIPYKLKHWLDIINQPGLTFRFDAASGYLPLLKDRPTLVILSSGGDFSTGMSRGRTDLATP
YLREALRFMGLRDVTFVAIGPTSGPAAHTGAARERAHRRLVEIAARF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=188, Percent_Identity=33.5106382978723, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 23244; Mature: 23113

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLLHLAASPRADSESGAASRVFLDRFRQARPGFDIDEMNLWREHLPEFDGEILQAKYA
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
RMAGRAFTDAQRAAFAVAERMAVRLDLAERVLISTPMWNFGIPYKLKHWLDIINQPGLTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEE
RFDAASGYLPLLKDRPTLVILSSGGDFSTGMSRGRTDLATPYLREALRFMGLRDVTFVAI
EECCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEE
GPTSGPAAHTGAARERAHRRLVEIAARF
CCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKLLHLAASPRADSESGAASRVFLDRFRQARPGFDIDEMNLWREHLPEFDGEILQAKYA
CCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
RMAGRAFTDAQRAAFAVAERMAVRLDLAERVLISTPMWNFGIPYKLKHWLDIINQPGLTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEE
RFDAASGYLPLLKDRPTLVILSSGGDFSTGMSRGRTDLATPYLREALRFMGLRDVTFVAI
EECCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEE
GPTSGPAAHTGAARERAHRRLVEIAARF
CCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]