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The map label for this gene is hpcB [H]

Identifier: 146341564

GI number: 146341564

Start: 4855101

End: 4856018

Strand: Reverse

Name: hpcB [H]

Synonym: BRADO4665

Alternate gene names: 146341564

Gene position: 4856018-4855101 (Counterclockwise)

Preceding gene: 146341565

Following gene: 146341561

Centisome position: 65.12

GC content: 66.12

Gene sequence:

>918_bases
ATGGGCAAGCTCGCACTGGCGGCCAAGATCACGCATGTGCCGTCGATGTATCTCAGCGAGCTCGATGGTCCGGCCAAGGG
CAAGCGGCAGTCGGCGATCGATGGCCACATCGAGATCGGGCGGCGCTGCCGCGAGGCCGGCGTCGACACGATCGTCGTGT
TCGACACCCATTGGCTGGTCAATGCCGGGTATCACATCAACTGCGCGCCGCACTTCCAGGGGCTCTACACCTCGAACGAG
CTGCCGAATTTCATCGCCAACATGACCTACGAATACGACGGCAATCCGGAGCTCGGCCACATCCTGGCCAAGGGCGCCAA
CGCGCATGGCGTCGCCACGATGGCCCATGACGCGACGTCGCTGGCGCTGGAATACGGCACGCTGGTGCCGATGCGCTACA
TGAACGCCGACCGCCACTTCAGGGTGGTGTCGGTCTCGGCGCTATGCACCTCGCATTATCTGGCCGACAGCGCCAGGCTC
GGCTGGGCATTCCGCCGCGCCGTCGAAGACCATTATGACGGCACGGTCGCGTTCTTCGCCTCGGGCTCTCTCAGCCACCG
CTTCGCGCAGAACGGCACCGCCGACGAGTATCGCGACAAGATGTTCAGCCCGTTCCTGGAGCGGCTCGACCACGAGGTGA
TCCAGATGTGGGAGCAGGGCGAATGGCCCGACTTCTGCGACATGCTGCCGGAATACGCCAGCAAGGGGCATGGCGAGGGA
TTCATGCACGATACCGCGATGCTGCTCGGCGCGCTCGGCTGGAACCACTACGATCGCGGTGTCGAGGTCGTCACGCCGTT
CTTCCCGTCCTCGGGCACCGGGCAGATCAACGCGGTGTTCCCGGTGGCGCCGATGCCGAGCGCGCTGGACGACATCGGCC
GGCCGCGCGCCGCCGCGCTGGCCGGCGGGCGGCTCTAG

Upstream 100 bases:

>100_bases
TCGCGGCCCTTTTTTTCGCCGTCGCGCCCGGCTAATCTGCGCGCCAAGCAAGGCGGTCCGCGACGCCGGACCGCGGAACG
GCGGGAAGGGAGACGAGGCA

Downstream 100 bases:

>100_bases
CGGATTCCGGCCTCACCAGACTCCGGCGCGCGCCAGCATGTCGATGATATCCTCGGGGAACACCTGATCGGTCGTGGCCG
CGAGCTCCGCGGGCCGCCAC

Product: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

Products: NA

Alternate protein names: Homoprotocatechuate 2,3-dioxygenase; HPC dioxygenase [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MGKLALAAKITHVPSMYLSELDGPAKGKRQSAIDGHIEIGRRCREAGVDTIVVFDTHWLVNAGYHINCAPHFQGLYTSNE
LPNFIANMTYEYDGNPELGHILAKGANAHGVATMAHDATSLALEYGTLVPMRYMNADRHFRVVSVSALCTSHYLADSARL
GWAFRRAVEDHYDGTVAFFASGSLSHRFAQNGTADEYRDKMFSPFLERLDHEVIQMWEQGEWPDFCDMLPEYASKGHGEG
FMHDTAMLLGALGWNHYDRGVEVVTPFFPSSGTGQINAVFPVAPMPSALDDIGRPRAAALAGGRL

Sequences:

>Translated_305_residues
MGKLALAAKITHVPSMYLSELDGPAKGKRQSAIDGHIEIGRRCREAGVDTIVVFDTHWLVNAGYHINCAPHFQGLYTSNE
LPNFIANMTYEYDGNPELGHILAKGANAHGVATMAHDATSLALEYGTLVPMRYMNADRHFRVVSVSALCTSHYLADSARL
GWAFRRAVEDHYDGTVAFFASGSLSHRFAQNGTADEYRDKMFSPFLERLDHEVIQMWEQGEWPDFCDMLPEYASKGHGEG
FMHDTAMLLGALGWNHYDRGVEVVTPFFPSSGTGQINAVFPVAPMPSALDDIGRPRAAALAGGRL
>Mature_304_residues
GKLALAAKITHVPSMYLSELDGPAKGKRQSAIDGHIEIGRRCREAGVDTIVVFDTHWLVNAGYHINCAPHFQGLYTSNEL
PNFIANMTYEYDGNPELGHILAKGANAHGVATMAHDATSLALEYGTLVPMRYMNADRHFRVVSVSALCTSHYLADSARLG
WAFRRAVEDHYDGTVAFFASGSLSHRFAQNGTADEYRDKMFSPFLERLDHEVIQMWEQGEWPDFCDMLPEYASKGHGEGF
MHDTAMLLGALGWNHYDRGVEVVTPFFPSSGTGQINAVFPVAPMPSALDDIGRPRAAALAGGRL

Specific function: Transforms homoprotocatechuic acid (HPC) into 5- carboxymethyl-2-hydroxy-muconic semialdehyde (CHMS) [H]

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011984
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.15 [H]

Molecular weight: Translated: 33549; Mature: 33418

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLALAAKITHVPSMYLSELDGPAKGKRQSAIDGHIEIGRRCREAGVDTIVVFDTHWLV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHCCCCEEEEEECCEEE
NAGYHINCAPHFQGLYTSNELPNFIANMTYEYDGNPELGHILAKGANAHGVATMAHDATS
ECCEEEEECCCCCCCCCCCCCCHHHHHCEEEECCCCCHHHHEECCCCCCCEEEECCCHHH
LALEYGTLVPMRYMNADRHFRVVSVSALCTSHYLADSARLGWAFRRAVEDHYDGTVAFFA
HHHHHCCCCCHHHCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEE
SGSLSHRFAQNGTADEYRDKMFSPFLERLDHEVIQMWEQGEWPDFCDMLPEYASKGHGEG
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
FMHDTAMLLGALGWNHYDRGVEVVTPFFPSSGTGQINAVFPVAPMPSALDDIGRPRAAAL
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHCCCHHHHH
AGGRL
CCCCC
>Mature Secondary Structure 
GKLALAAKITHVPSMYLSELDGPAKGKRQSAIDGHIEIGRRCREAGVDTIVVFDTHWLV
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHCCCCEEEEEECCEEE
NAGYHINCAPHFQGLYTSNELPNFIANMTYEYDGNPELGHILAKGANAHGVATMAHDATS
ECCEEEEECCCCCCCCCCCCCCHHHHHCEEEECCCCCHHHHEECCCCCCCEEEECCCHHH
LALEYGTLVPMRYMNADRHFRVVSVSALCTSHYLADSARLGWAFRRAVEDHYDGTVAFFA
HHHHHCCCCCHHHCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEE
SGSLSHRFAQNGTADEYRDKMFSPFLERLDHEVIQMWEQGEWPDFCDMLPEYASKGHGEG
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
FMHDTAMLLGALGWNHYDRGVEVVTPFFPSSGTGQINAVFPVAPMPSALDDIGRPRAAAL
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHCCCHHHHH
AGGRL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2261999 [H]