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The map label for this gene is gntR [H]

Identifier: 146340095

GI number: 146340095

Start: 3300385

End: 3301479

Strand: Reverse

Name: gntR [H]

Synonym: BRADO3104

Alternate gene names: 146340095

Gene position: 3301479-3300385 (Counterclockwise)

Preceding gene: 146340102

Following gene: 161353766

Centisome position: 44.28

GC content: 64.11

Gene sequence:

>1095_bases
ATGGGACGGAAACAGATCAACCCGGGCAAAGTCCGGCTGACCGAGGTTGCAGCACTGGCGGGCGTCAGCCCGATCACGGT
GTCACGCTTCTTCCGTAATCCCGACACCGTCTCGGCGGGCCGCCGCCTGCGCATCGAGAGTGCCGCCAAGGATCTCGGCT
ACGTTCCCAACCTCGCCGCGCGCGCGCTCGCATCGCAGCGCACCGAAGTGATCGGCGTGCTGATACCGTCGCTGACCAAC
AACGTGTTCTCCGACGTGCTGCGCGGCATCTATGACGCGTTCGAAGGCAGCCGCTACAGCATCCAACTTGCGAATACACG
CTATAGTGTGCTGCAGGAGGAGCGGCTGCTGCGGCTGTTCCTGATGCAGAAGCCGGCCGGCCTGATCGTGACGGGCGTCG
ACCAGACCGCAGAAGGCCGCTCCCTCGTGCAGGCGGCCGACTGTCCGGTGGTGCAGATCATGGAGCTGGGTGCCGATCCG
ATCGACATGATGATCGGGTTTTCGCATGTGGACGCTTGCTTCGCCGCGATTTCTCACCTTCTCGCCCAGGGTTGCCGGCG
CATCGGATTTCTCGGCGCACGCATGGATCCCAGGGTGCAGCGTCGCCTTCAGGGCTACCAGAACGCCATGAAGAATGCCG
GACTGTTCGATCCGCGACTGATCGTCACAACCGCCGTGCCGACGTCCACGACCGTGGGCGGCAGCCTGTTCTCGGATCTC
ATGCTGAGGGCGCCCGATCTCGATGCCGTATTCTGCGCCAATGACGACCTCGCGCTCGGGGTGCTGTTCGAGTGCCAGCG
GCGCCACATCGCGGTGCCCGAGCAGTTGGCGATCGTCGGCTTCAACGACATGGAGTTCATGGCATCGGCCGTTCCGACGC
TGACCAGCGTCAGGACCAACCGCTACGAGATGGGCCTGCGCGCGGCGAAGATGATCATGGACGAGGTCGAAGGCCGGCCT
GTCGCCGAGCGCGTGGTCGATCTCGGATTCGAGGTGATGGAGCGGCAGAGCTCGACCTTGCAGCGCGAGGTCGGAAGCGC
CGGGCGAGCGGGCCCCAGAAACAAAATGGTAGCGCAACCAAAATCGGTGATCTAG

Upstream 100 bases:

>100_bases
TCGGCAGTTTAGTTCTCTTTCGCAATGCTTCGCTTAGCAACCGCGTGATAGCGCTGTCAATATCGCTTGTGTACCAGACC
GCCTATGCTATGAGCGACGG

Downstream 100 bases:

>100_bases
AACCACGCGAACGAACGAGACCTTGAACCAAAGGCGTAGGCGAGCTGCGACGCCGGCTGGCGGCATCTGACTGCGGCGCC
TCATCCCGCGTGATCCCCAC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MGRKQINPGKVRLTEVAALAGVSPITVSRFFRNPDTVSAGRRLRIESAAKDLGYVPNLAARALASQRTEVIGVLIPSLTN
NVFSDVLRGIYDAFEGSRYSIQLANTRYSVLQEERLLRLFLMQKPAGLIVTGVDQTAEGRSLVQAADCPVVQIMELGADP
IDMMIGFSHVDACFAAISHLLAQGCRRIGFLGARMDPRVQRRLQGYQNAMKNAGLFDPRLIVTTAVPTSTTVGGSLFSDL
MLRAPDLDAVFCANDDLALGVLFECQRRHIAVPEQLAIVGFNDMEFMASAVPTLTSVRTNRYEMGLRAAKMIMDEVEGRP
VAERVVDLGFEVMERQSSTLQREVGSAGRAGPRNKMVAQPKSVI

Sequences:

>Translated_364_residues
MGRKQINPGKVRLTEVAALAGVSPITVSRFFRNPDTVSAGRRLRIESAAKDLGYVPNLAARALASQRTEVIGVLIPSLTN
NVFSDVLRGIYDAFEGSRYSIQLANTRYSVLQEERLLRLFLMQKPAGLIVTGVDQTAEGRSLVQAADCPVVQIMELGADP
IDMMIGFSHVDACFAAISHLLAQGCRRIGFLGARMDPRVQRRLQGYQNAMKNAGLFDPRLIVTTAVPTSTTVGGSLFSDL
MLRAPDLDAVFCANDDLALGVLFECQRRHIAVPEQLAIVGFNDMEFMASAVPTLTSVRTNRYEMGLRAAKMIMDEVEGRP
VAERVVDLGFEVMERQSSTLQREVGSAGRAGPRNKMVAQPKSVI
>Mature_363_residues
GRKQINPGKVRLTEVAALAGVSPITVSRFFRNPDTVSAGRRLRIESAAKDLGYVPNLAARALASQRTEVIGVLIPSLTNN
VFSDVLRGIYDAFEGSRYSIQLANTRYSVLQEERLLRLFLMQKPAGLIVTGVDQTAEGRSLVQAADCPVVQIMELGADPI
DMMIGFSHVDACFAAISHLLAQGCRRIGFLGARMDPRVQRRLQGYQNAMKNAGLFDPRLIVTTAVPTSTTVGGSLFSDLM
LRAPDLDAVFCANDDLALGVLFECQRRHIAVPEQLAIVGFNDMEFMASAVPTLTSVRTNRYEMGLRAAKMIMDEVEGRPV
AERVVDLGFEVMERQSSTLQREVGSAGRAGPRNKMVAQPKSVI

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=318, Percent_Identity=38.0503144654088, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1790715, Length=328, Percent_Identity=33.8414634146341, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1790369, Length=312, Percent_Identity=30.7692307692308, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=27.8787878787879, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1789068, Length=311, Percent_Identity=28.9389067524116, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789202, Length=322, Percent_Identity=27.9503105590062, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1788474, Length=304, Percent_Identity=27.6315789473684, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787948, Length=337, Percent_Identity=22.8486646884273, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1786540, Length=317, Percent_Identity=26.4984227129338, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=24.6987951807229, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787906, Length=336, Percent_Identity=22.9166666666667, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39730; Mature: 39598

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRKQINPGKVRLTEVAALAGVSPITVSRFFRNPDTVSAGRRLRIESAAKDLGYVPNLAA
CCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEHHHHHHHCCCCCCHHH
RALASQRTEVIGVLIPSLTNNVFSDVLRGIYDAFEGSRYSIQLANTRYSVLQEERLLRLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHH
LMQKPAGLIVTGVDQTAEGRSLVQAADCPVVQIMELGADPIDMMIGFSHVDACFAAISHL
HHCCCCCEEEECCCCCCCCHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LAQGCRRIGFLGARMDPRVQRRLQGYQNAMKNAGLFDPRLIVTTAVPTSTTVGGSLFSDL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHH
MLRAPDLDAVFCANDDLALGVLFECQRRHIAVPEQLAIVGFNDMEFMASAVPTLTSVRTN
HHCCCCCCEEEECCCCCEEHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
RYEMGLRAAKMIMDEVEGRPVAERVVDLGFEVMERQSSTLQREVGSAGRAGPRNKMVAQP
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KSVI
CCCC
>Mature Secondary Structure 
GRKQINPGKVRLTEVAALAGVSPITVSRFFRNPDTVSAGRRLRIESAAKDLGYVPNLAA
CCCCCCCCCEEHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEHHHHHHHCCCCCCHHH
RALASQRTEVIGVLIPSLTNNVFSDVLRGIYDAFEGSRYSIQLANTRYSVLQEERLLRLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHH
LMQKPAGLIVTGVDQTAEGRSLVQAADCPVVQIMELGADPIDMMIGFSHVDACFAAISHL
HHCCCCCEEEECCCCCCCCHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LAQGCRRIGFLGARMDPRVQRRLQGYQNAMKNAGLFDPRLIVTTAVPTSTTVGGSLFSDL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHH
MLRAPDLDAVFCANDDLALGVLFECQRRHIAVPEQLAIVGFNDMEFMASAVPTLTSVRTN
HHCCCCCCEEEECCCCCEEHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
RYEMGLRAAKMIMDEVEGRPVAERVVDLGFEVMERQSSTLQREVGSAGRAGPRNKMVAQP
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KSVI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]