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The map label for this gene is yvrD [H]

Identifier: 146339542

GI number: 146339542

Start: 2706513

End: 2707289

Strand: Reverse

Name: yvrD [H]

Synonym: BRADO2533

Alternate gene names: 146339542

Gene position: 2707289-2706513 (Counterclockwise)

Preceding gene: 146339544

Following gene: 146339541

Centisome position: 36.31

GC content: 67.44

Gene sequence:

>777_bases
ATGGATCTTCATCTGCGCGGCAAGCGCGTTCTCATCACCGGCGCCTCCAAGGGCATCGGCGCCGCAGCGGCCGAGGTGTT
CGCCGAGGAAGGCTGTCATGTCAGCCTTGCGGCGCGCAGCGGCGACCGGCTCGCGGCGCTGGTCGAGCAACTGCGCTCGA
CGCACCAGATCGACGCAAGGGCGCACGTCGTCGACCTGCGCAAATCCGAAGATGTGGCGCGGCTCGCGCAGGAGGCCGCC
GACATCGACATCCTCGTCAACAATGCCGGCGACATTCCCGGCGGCCCGATCGACAAGATCGACGAGGCGACCTGGCGGCA
CGCCTGGGACCTCAAGGTATTCGGCTTCATCAACCTCGCCCGGCTGGTCTACGCCCGGATGAAGGCGCGCGGCGCCGGCG
TGATCATCAACGACATCGGCGCGGCCGGAGAGAAGTTCGATTTCAACTACATCTGCGGCAGCGCCGGCAACGCGGCGCTG
ATGGCCTTCACCCGCGCGCTCGGCTCCCAGAGCCTCGCCGACAACATCCGCGTCGTCGGCATCAATCCCGGCCCGGTCGG
CACCGACCGCCACGTCACCCTGCTGAAGACCCGTGCCAAACAGCAGTTCGGCGACGAAAGCCGCTACACGGAATTCCAGA
AAGGCCTGCCGCTCGGCCGCCCCGCGCACGCCAGAGAGATCGCCGACCTGATGGCGTTCCTGGCATCCGACCGCGCTGGC
TACACCTCGGGCGTGATCTATACCGTCGATGGCGGCATCAGCGCGGGGTGGGGTTGA

Upstream 100 bases:

>100_bases
TGGACCAAACCGGAGGCAAGCGCAACGCTGCGGTGCAAGATTGACCGCATGGCTGGCGCGCAAGACAATCCGGTTTCCCT
CCCCTCAACGAGAGCCCTCC

Downstream 100 bases:

>100_bases
GCGCAGCGATCTTTCCACACCGGACGAAACGTGGTCTACAGCACGGCGCCGGAACGCTTCCAATCAGGAGAACCACGCCT
TGGCCGACCAGGAATTGATC

Product: short chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MDLHLRGKRVLITGASKGIGAAAAEVFAEEGCHVSLAARSGDRLAALVEQLRSTHQIDARAHVVDLRKSEDVARLAQEAA
DIDILVNNAGDIPGGPIDKIDEATWRHAWDLKVFGFINLARLVYARMKARGAGVIINDIGAAGEKFDFNYICGSAGNAAL
MAFTRALGSQSLADNIRVVGINPGPVGTDRHVTLLKTRAKQQFGDESRYTEFQKGLPLGRPAHAREIADLMAFLASDRAG
YTSGVIYTVDGGISAGWG

Sequences:

>Translated_258_residues
MDLHLRGKRVLITGASKGIGAAAAEVFAEEGCHVSLAARSGDRLAALVEQLRSTHQIDARAHVVDLRKSEDVARLAQEAA
DIDILVNNAGDIPGGPIDKIDEATWRHAWDLKVFGFINLARLVYARMKARGAGVIINDIGAAGEKFDFNYICGSAGNAAL
MAFTRALGSQSLADNIRVVGINPGPVGTDRHVTLLKTRAKQQFGDESRYTEFQKGLPLGRPAHAREIADLMAFLASDRAG
YTSGVIYTVDGGISAGWG
>Mature_258_residues
MDLHLRGKRVLITGASKGIGAAAAEVFAEEGCHVSLAARSGDRLAALVEQLRSTHQIDARAHVVDLRKSEDVARLAQEAA
DIDILVNNAGDIPGGPIDKIDEATWRHAWDLKVFGFINLARLVYARMKARGAGVIINDIGAAGEKFDFNYICGSAGNAAL
MAFTRALGSQSLADNIRVVGINPGPVGTDRHVTLLKTRAKQQFGDESRYTEFQKGLPLGRPAHAREIADLMAFLASDRAG
YTSGVIYTVDGGISAGWG

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=256, Percent_Identity=25.78125, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI10190704, Length=252, Percent_Identity=28.1746031746032, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI66933014, Length=252, Percent_Identity=30.5555555555556, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI32483357, Length=256, Percent_Identity=28.515625, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI19923817, Length=258, Percent_Identity=26.7441860465116, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI4503817, Length=190, Percent_Identity=27.3684210526316, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI5031737, Length=246, Percent_Identity=25.609756097561, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787905, Length=257, Percent_Identity=31.5175097276265, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786812, Length=263, Percent_Identity=28.8973384030418, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=31.6602316602317, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1787335, Length=255, Percent_Identity=30.9803921568627, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790717, Length=255, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789208, Length=257, Percent_Identity=26.4591439688716, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788459, Length=247, Percent_Identity=25.5060728744939, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI2367175, Length=258, Percent_Identity=25.968992248062, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082160, Length=268, Percent_Identity=26.1194029850746, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787820, Length=185, Percent_Identity=32.4324324324324, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=261, Percent_Identity=28.3524904214559, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71994604, Length=253, Percent_Identity=29.6442687747036, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71994600, Length=254, Percent_Identity=29.5275590551181, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI115534694, Length=249, Percent_Identity=31.3253012048193, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538480, Length=272, Percent_Identity=30.1470588235294, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562990, Length=262, Percent_Identity=29.0076335877863, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17560676, Length=257, Percent_Identity=28.7937743190661, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17562910, Length=265, Percent_Identity=27.5471698113208, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17536025, Length=255, Percent_Identity=27.4509803921569, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560150, Length=261, Percent_Identity=28.735632183908, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI72000259, Length=262, Percent_Identity=27.4809160305344, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25147288, Length=260, Percent_Identity=25.7692307692308, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=266, Percent_Identity=31.203007518797, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17563726, Length=259, Percent_Identity=25.4826254826255, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=266, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=270, Percent_Identity=28.1481481481481, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17536651, Length=254, Percent_Identity=25.1968503937008, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=265, Percent_Identity=27.9245283018868, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17544670, Length=272, Percent_Identity=26.4705882352941, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193204405, Length=277, Percent_Identity=29.9638989169675, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=247, Percent_Identity=32.3886639676113, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI23397609, Length=256, Percent_Identity=31.640625, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24644339, Length=255, Percent_Identity=30.1960784313725, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21357041, Length=254, Percent_Identity=29.1338582677165, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28571526, Length=256, Percent_Identity=27.734375, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24644337, Length=262, Percent_Identity=31.2977099236641, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355319, Length=260, Percent_Identity=26.5384615384615, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24643142, Length=250, Percent_Identity=26, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21358495, Length=204, Percent_Identity=32.3529411764706, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 27508; Mature: 27508

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLHLRGKRVLITGASKGIGAAAAEVFAEEGCHVSLAARSGDRLAALVEQLRSTHQIDAR
CCCEECCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCH
AHVVDLRKSEDVARLAQEAADIDILVNNAGDIPGGPIDKIDEATWRHAWDLKVFGFINLA
HEEEECCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHEECCCHHHHHHHHH
RLVYARMKARGAGVIINDIGAAGEKFDFNYICGSAGNAALMAFTRALGSQSLADNIRVVG
HHHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHCCEEEEE
INPGPVGTDRHVTLLKTRAKQQFGDESRYTEFQKGLPLGRPAHAREIADLMAFLASDRAG
ECCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
YTSGVIYTVDGGISAGWG
CCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MDLHLRGKRVLITGASKGIGAAAAEVFAEEGCHVSLAARSGDRLAALVEQLRSTHQIDAR
CCCEECCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCH
AHVVDLRKSEDVARLAQEAADIDILVNNAGDIPGGPIDKIDEATWRHAWDLKVFGFINLA
HEEEECCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHEECCCHHHHHHHHH
RLVYARMKARGAGVIINDIGAAGEKFDFNYICGSAGNAALMAFTRALGSQSLADNIRVVG
HHHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHCCEEEEE
INPGPVGTDRHVTLLKTRAKQQFGDESRYTEFQKGLPLGRPAHAREIADLMAFLASDRAG
ECCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
YTSGVIYTVDGGISAGWG
CCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]