BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is dkgB [H]

Identifier: 146339169

GI number: 146339169

Start: 2263425

End: 2264246

Strand: Direct

Name: dkgB [H]

Synonym: BRADO2128

Alternate gene names: 146339169

Gene position: 2263425-2264246 (Clockwise)

Preceding gene: 146339168

Following gene: 146339172

Centisome position: 30.35

GC content: 66.42

Gene sequence:

>822_bases
ATGAACGTCATCGAAACGCAGGGCGTCCGGCTGCCCAAGCTCGGGCTCGGCACCTTCCGCATGCAGGGCACAGTATGCCG
CGACGCGGTCGAGAGCGCGCTCGCGCTCGGCTACCGCCACATCGACACCGCCGAGATGTACGCCAATGAGGATGCGATCG
GCGCGGCGCTTGCGGCAGCTCAGATCGCCCGCGAGGATTTGCACGTCACGACAAAGGTCTGGCCGGAGAACCTCGCGCCG
GACGCCATCCGCCGCGCGTTCGACACCAGCTTGAAGAAGCTCAGGCTCGACTTCGTCGATCTCTATCTCATTCACTGGCC
GGCGACGGGCATGAACCTGCCGGCCGCGATGGAGACGTTGATGGCGCTGAAGCAGGAGGGACGCACGCGCGCGATCGGCG
TCGCCAACTTCACCGTCGCGCTGCTCAAGCAGGCCGTCGAGGACATCCAGGCGCCGGTTGCCTGCAATCAGGTCGAGTAT
CACGTGATGCTCGATCAGTCGAAGCTGATGGCCTACATGAAGAGCCGCTCGATTCCCCTGGTGGCCTACTGCCCCCTCGC
ACAGGGCCGGGCGGCCGGCGACGAGACGCTGGCCGGGATCGGCCGCAAGCACGGCGCTGGCGCCGCCCAGGTCGCGCTGA
AATGGCTGCTCGACCAGGATGGCGTCGCCGCCATCCCGAAGGCCGCGCGCCCGGAGAGTCAGCAGGCGAACTGGGATGCG
ATGAAGGTTCAACTGGATGATGAGGACCGCGCGACCATCGCCGCGTTGCCGAAGGACAAGCGGTTCGTCAATCCGGGCTT
CGCGCCGGATTGGGATCGCTGA

Upstream 100 bases:

>100_bases
GGGCCTCAGGCAGTCTGAGCGTATCCCGTCAAGGTCTTGATATACCAAGGCTGCGCCAGTGCGAATCTGGCCACAGGTTT
GCGTCGTCCCAGGAGAGATC

Downstream 100 bases:

>100_bases
ACGTTCGTCGGCGACAAACTCTGCGCAGAAAAATGGCCCCTCGCGGGGCCATTTCCATCTCTGCGGCGCGGATCAGTCGC
GGCGCTTGATGATCACGGTG

Product: 2,5-diketo-D-gluconate reductase B

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNVIETQGVRLPKLGLGTFRMQGTVCRDAVESALALGYRHIDTAEMYANEDAIGAALAAAQIAREDLHVTTKVWPENLAP
DAIRRAFDTSLKKLRLDFVDLYLIHWPATGMNLPAAMETLMALKQEGRTRAIGVANFTVALLKQAVEDIQAPVACNQVEY
HVMLDQSKLMAYMKSRSIPLVAYCPLAQGRAAGDETLAGIGRKHGAGAAQVALKWLLDQDGVAAIPKAARPESQQANWDA
MKVQLDDEDRATIAALPKDKRFVNPGFAPDWDR

Sequences:

>Translated_273_residues
MNVIETQGVRLPKLGLGTFRMQGTVCRDAVESALALGYRHIDTAEMYANEDAIGAALAAAQIAREDLHVTTKVWPENLAP
DAIRRAFDTSLKKLRLDFVDLYLIHWPATGMNLPAAMETLMALKQEGRTRAIGVANFTVALLKQAVEDIQAPVACNQVEY
HVMLDQSKLMAYMKSRSIPLVAYCPLAQGRAAGDETLAGIGRKHGAGAAQVALKWLLDQDGVAAIPKAARPESQQANWDA
MKVQLDDEDRATIAALPKDKRFVNPGFAPDWDR
>Mature_273_residues
MNVIETQGVRLPKLGLGTFRMQGTVCRDAVESALALGYRHIDTAEMYANEDAIGAALAAAQIAREDLHVTTKVWPENLAP
DAIRRAFDTSLKKLRLDFVDLYLIHWPATGMNLPAAMETLMALKQEGRTRAIGVANFTVALLKQAVEDIQAPVACNQVEY
HVMLDQSKLMAYMKSRSIPLVAYCPLAQGRAAGDETLAGIGRKHGAGAAQVALKWLLDQDGVAAIPKAARPESQQANWDA
MKVQLDDEDRATIAALPKDKRFVNPGFAPDWDR

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI310109922, Length=276, Percent_Identity=32.2463768115942, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI310109920, Length=295, Percent_Identity=31.1864406779661, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI24497585, Length=306, Percent_Identity=31.6993464052288, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI45446745, Length=295, Percent_Identity=31.1864406779661, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI4503285, Length=295, Percent_Identity=31.1864406779661, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI223468663, Length=293, Percent_Identity=31.740614334471, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI5453543, Length=295, Percent_Identity=30.8474576271186, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI5174695, Length=299, Percent_Identity=30.7692307692308, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI24497583, Length=296, Percent_Identity=30.4054054054054, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI24497577, Length=305, Percent_Identity=28.8524590163934, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI5174391, Length=305, Percent_Identity=28.8524590163934, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI291291012, Length=271, Percent_Identity=31.3653136531365, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI300116273, Length=278, Percent_Identity=29.8561151079137, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI300116271, Length=277, Percent_Identity=29.2418772563177, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI93277124, Length=304, Percent_Identity=26.9736842105263, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4502049, Length=294, Percent_Identity=30.2721088435374, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI310109926, Length=109, Percent_Identity=39.4495412844037, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI207028673, Length=109, Percent_Identity=39.4495412844037, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI310109924, Length=153, Percent_Identity=30.0653594771242, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786400, Length=266, Percent_Identity=42.1052631578947, Blast_Score=217, Evalue=7e-58,
Organism=Escherichia coli, GI87082198, Length=264, Percent_Identity=35.9848484848485, Blast_Score=167, Evalue=5e-43,
Organism=Escherichia coli, GI1788081, Length=278, Percent_Identity=34.1726618705036, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788070, Length=311, Percent_Identity=26.3665594855305, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787674, Length=282, Percent_Identity=25.886524822695, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081735, Length=287, Percent_Identity=26.4808362369338, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789375, Length=296, Percent_Identity=25.3378378378378, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71998625, Length=263, Percent_Identity=31.5589353612167, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17537077, Length=293, Percent_Identity=33.7883959044369, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17537075, Length=294, Percent_Identity=33.6734693877551, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17566692, Length=293, Percent_Identity=30.0341296928328, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17561298, Length=275, Percent_Identity=29.0909090909091, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17561300, Length=281, Percent_Identity=31.6725978647687, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17538386, Length=275, Percent_Identity=30.9090909090909, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17564128, Length=300, Percent_Identity=31, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17552492, Length=264, Percent_Identity=28.7878787878788, Blast_Score=127, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17550248, Length=294, Percent_Identity=30.2721088435374, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17537079, Length=302, Percent_Identity=26.158940397351, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17562292, Length=288, Percent_Identity=29.5138888888889, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324694, Length=295, Percent_Identity=33.2203389830508, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320576, Length=283, Percent_Identity=32.5088339222615, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6321896, Length=312, Percent_Identity=27.5641025641026, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6319625, Length=302, Percent_Identity=30.1324503311258, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6322556, Length=255, Percent_Identity=26.6666666666667, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320079, Length=233, Percent_Identity=27.4678111587983, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356425, Length=296, Percent_Identity=33.445945945946, Blast_Score=160, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24644950, Length=303, Percent_Identity=33.6633663366337, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI221378297, Length=323, Percent_Identity=31.5789473684211, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24657054, Length=292, Percent_Identity=33.2191780821918, Blast_Score=151, Evalue=4e-37,
Organism=Drosophila melanogaster, GI45553081, Length=292, Percent_Identity=32.1917808219178, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI20129731, Length=285, Percent_Identity=30.5263157894737, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24663317, Length=293, Percent_Identity=29.0102389078498, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24662789, Length=301, Percent_Identity=29.9003322259136, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI281366140, Length=261, Percent_Identity=31.4176245210728, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24662785, Length=279, Percent_Identity=31.1827956989247, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24662781, Length=280, Percent_Identity=31.0714285714286, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 29834; Mature: 29834

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVIETQGVRLPKLGLGTFRMQGTVCRDAVESALALGYRHIDTAEMYANEDAIGAALAAA
CCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHH
QIAREDLHVTTKVWPENLAPDAIRRAFDTSLKKLRLDFVDLYLIHWPATGMNLPAAMETL
HHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHH
MALKQEGRTRAIGVANFTVALLKQAVEDIQAPVACNQVEYHVMLDQSKLMAYMKSRSIPL
HHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHCCCCE
VAYCPLAQGRAAGDETLAGIGRKHGAGAAQVALKWLLDQDGVAAIPKAARPESQQANWDA
EEECCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCE
MKVQLDDEDRATIAALPKDKRFVNPGFAPDWDR
EEEEECCCCCEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNVIETQGVRLPKLGLGTFRMQGTVCRDAVESALALGYRHIDTAEMYANEDAIGAALAAA
CCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHH
QIAREDLHVTTKVWPENLAPDAIRRAFDTSLKKLRLDFVDLYLIHWPATGMNLPAAMETL
HHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHH
MALKQEGRTRAIGVANFTVALLKQAVEDIQAPVACNQVEYHVMLDQSKLMAYMKSRSIPL
HHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHCCCCE
VAYCPLAQGRAAGDETLAGIGRKHGAGAAQVALKWLLDQDGVAAIPKAARPESQQANWDA
EEECCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCE
MKVQLDDEDRATIAALPKDKRFVNPGFAPDWDR
EEEEECCCCCEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796; 11108008 [H]