| Definition | Bradyrhizobium sp. ORS278 chromosome, complete genome. |
|---|---|
| Accession | NC_009445 |
| Length | 7,456,587 |
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The map label for this gene is hupV [C]
Identifier: 146338749
GI number: 146338749
Start: 1800154
End: 1801593
Strand: Direct
Name: hupV [C]
Synonym: BRADO1683
Alternate gene names: 146338749
Gene position: 1800154-1801593 (Clockwise)
Preceding gene: 146338748
Following gene: 146338750
Centisome position: 24.14
GC content: 67.5
Gene sequence:
>1440_bases ATGACGCGGATCACGGTCGGCCCGTTCAATCGCGTCGAGGGGGATCTCGAGGTCCGTCTCGACATCGAGAGCGGCCGCGT CGCGCGCGCCGAGGTGACGGCGCCGCTCTATCGCGGCTTCGAGCAGATCCTGGAAGGACGTCCGCCGCTCGACGCCCTCG TGATCGCCCCGCGCATCTGCGGCATCTGCTCGGTGTCGCAGTCGATCGCTGCCGCAGCCGCCCTGCGTCAGGCGATGGGC GTGGTCGTGGCGCCGAATGGCGTCCTGGCGACCAACATCGCCCACGCGGCTGAGAATGCCGCGGATCACCTCACGCACTT CTACATCTTCTTCATGCCCGACTTCGCCCGCGAGGCCTACGCGTCTCACTCTTGGCACGGTGCGGCGGCCGAACGCTTCA CCGCGACGAAGGGCTCGGCCGCGCGCGAGGCGCTGCCGGCGCGGGCTCGGCTGCTGGAAATCATGGGTGTGATCGCCGGG AAATGGCCGCACAGCCTCGCGTTCCAGCCGGGCGGTACGACGCGCGCCATCGAGCTCGGTGAGCGCGTCCGGCTGAACGC GATCGTGGCTTCGTTTCGCGGCTTTCTCGAGCGTGTCGTATTTGCCGACAAGCTCGAACACGTGCTGGCGTTGTCGACGC CGGACGAGCTCGATCGCTGGCGTGACGGCCGTAGCGGCGACTTCGCGCAGTTCCTGCGCATCGCAGACGCGCTCGATCTG GCGTATCTCGGTGCGGGGCCCGGCCTGCTGATGAGCTTCGGTGCGTATCGCGATGCCGATGGCGCGCGCTTCCGCGCCGG GCTTCGAACGCCTGATGGAAAGATCGAAGCGCTGCCGTCGGGCCAGATCACCGAGGACGTGTCACATGCGTGGATGCGAG AGACGTCGCGCGATCCCGCGGAAAGCCGCACCGAGCCGGACGCCGACAAGCCCGATGCCTACAGCTGGTGCAAAGCACCG CGGCTTGCGGGTGAGCCGGTCGAGGTTGGCGCGTTGGCACGTCAAGCGGTGCACGGACAACAGCTGATCACGGCACTGCT TTCCGAAAGCGGCAGCAATGTCCGCAACCGCGTCATTGCCCGCCTGATCGAGACAGCCGAGCTTGCAGTCTCGATTGAAG CTTGGATCAAGGCACTGCGGCTCAACGAGCCGTTCTGCGAAACGAGCCGCGAGGCGGCCGATGGCAACTACGTCGGCCTC GTTGAGGCCGCACGCGGCAGCCTCGGCCATTGGCTCGCAGTGCGCTCCGGCAGGATCGCGCGCTATCAGATCATCGCGCC GACCAGCTGGAACTTTTCGCCACGCGACGCCCGGGGCGTCGCGGGTCCACTCGAACAGGCGCTGGTCGGCACGGAGGTCG GCGAGGCTGGCGCGCGTGCCGTCAGCATCCAGCATGTCGTACGGTCGTTCGATCCCTGCATGGTCTGCACCGCGCATTGA
Upstream 100 bases:
>100_bases CCGCGCTGTCGAAATCGGCGACACCGCGCCGCGTCCGCGTCAACGCGACCGCCGATCATGTCGTGGTGCCGCCGAGCCGG TCCGGCGACAAGCATCGCTC
Downstream 100 bases:
>100_bases CCCCGCTGCAAAGGGCGGAAACAAGCTTGTCGGGTGCAAAGCGCGTGATTTCCGATATTCGGCCGATCAACGATCCTGAA CTGCTTGTCGATCTCCAACC
Product: uptake hydrogenase accessory protein HupV
Products: NA
Alternate protein names: NiFeSe hydrogenlyase large chain [H]
Number of amino acids: Translated: 479; Mature: 478
Protein sequence:
>479_residues MTRITVGPFNRVEGDLEVRLDIESGRVARAEVTAPLYRGFEQILEGRPPLDALVIAPRICGICSVSQSIAAAAALRQAMG VVVAPNGVLATNIAHAAENAADHLTHFYIFFMPDFAREAYASHSWHGAAAERFTATKGSAAREALPARARLLEIMGVIAG KWPHSLAFQPGGTTRAIELGERVRLNAIVASFRGFLERVVFADKLEHVLALSTPDELDRWRDGRSGDFAQFLRIADALDL AYLGAGPGLLMSFGAYRDADGARFRAGLRTPDGKIEALPSGQITEDVSHAWMRETSRDPAESRTEPDADKPDAYSWCKAP RLAGEPVEVGALARQAVHGQQLITALLSESGSNVRNRVIARLIETAELAVSIEAWIKALRLNEPFCETSREAADGNYVGL VEAARGSLGHWLAVRSGRIARYQIIAPTSWNFSPRDARGVAGPLEQALVGTEVGEAGARAVSIQHVVRSFDPCMVCTAH
Sequences:
>Translated_479_residues MTRITVGPFNRVEGDLEVRLDIESGRVARAEVTAPLYRGFEQILEGRPPLDALVIAPRICGICSVSQSIAAAAALRQAMG VVVAPNGVLATNIAHAAENAADHLTHFYIFFMPDFAREAYASHSWHGAAAERFTATKGSAAREALPARARLLEIMGVIAG KWPHSLAFQPGGTTRAIELGERVRLNAIVASFRGFLERVVFADKLEHVLALSTPDELDRWRDGRSGDFAQFLRIADALDL AYLGAGPGLLMSFGAYRDADGARFRAGLRTPDGKIEALPSGQITEDVSHAWMRETSRDPAESRTEPDADKPDAYSWCKAP RLAGEPVEVGALARQAVHGQQLITALLSESGSNVRNRVIARLIETAELAVSIEAWIKALRLNEPFCETSREAADGNYVGL VEAARGSLGHWLAVRSGRIARYQIIAPTSWNFSPRDARGVAGPLEQALVGTEVGEAGARAVSIQHVVRSFDPCMVCTAH >Mature_478_residues TRITVGPFNRVEGDLEVRLDIESGRVARAEVTAPLYRGFEQILEGRPPLDALVIAPRICGICSVSQSIAAAAALRQAMGV VVAPNGVLATNIAHAAENAADHLTHFYIFFMPDFAREAYASHSWHGAAAERFTATKGSAAREALPARARLLEIMGVIAGK WPHSLAFQPGGTTRAIELGERVRLNAIVASFRGFLERVVFADKLEHVLALSTPDELDRWRDGRSGDFAQFLRIADALDLA YLGAGPGLLMSFGAYRDADGARFRAGLRTPDGKIEALPSGQITEDVSHAWMRETSRDPAESRTEPDADKPDAYSWCKAPR LAGEPVEVGALARQAVHGQQLITALLSESGSNVRNRVIARLIETAELAVSIEAWIKALRLNEPFCETSREAADGNYVGLV EAARGSLGHWLAVRSGRIARYQIIAPTSWNFSPRDARGVAGPLEQALVGTEVGEAGARAVSIQHVVRSFDPCMVCTAH
Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]
COG id: COG0374
COG function: function code C; Ni,Fe-hydrogenase I large subunit
Gene ontology:
Cell location: Periplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]
Homologues:
Organism=Escherichia coli, GI1789368, Length=565, Percent_Identity=27.4336283185841, Blast_Score=187, Evalue=1e-48, Organism=Escherichia coli, GI1787207, Length=589, Percent_Identity=27.1646859083192, Blast_Score=169, Evalue=3e-43,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001501 - InterPro: IPR018194 [H]
Pfam domain/function: PF00374 NiFeSe_Hases [H]
EC number: =1.12.99.6 [H]
Molecular weight: Translated: 51714; Mature: 51583
Theoretical pI: Translated: 6.65; Mature: 6.65
Prosite motif: PS00507 NI_HGENASE_L_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 1.3 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTRITVGPFNRVEGDLEVRLDIESGRVARAEVTAPLYRGFEQILEGRPPLDALVIAPRIC CCEEECCCHHHCCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH GICSVSQSIAAAAALRQAMGVVVAPNGVLATNIAHAAENAADHLTHFYIFFMPDFAREAY HHHHHHHHHHHHHHHHHHCCEEECCCCEEHHHHHHHHHHHHHHHHEEEEEECCHHHHHHH ASHSWHGAAAERFTATKGSAAREALPARARLLEIMGVIAGKWPHSLAFQPGGTTRAIELG HCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHC ERVRLNAIVASFRGFLERVVFADKLEHVLALSTPDELDRWRDGRSGDFAQFLRIADALDL CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHH AYLGAGPGLLMSFGAYRDADGARFRAGLRTPDGKIEALPSGQITEDVSHAWMRETSRDPA HHHCCCCHHHHHHCCCCCCCCCCEECCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCC ESRTEPDADKPDAYSWCKAPRLAGEPVEVGALARQAVHGQQLITALLSESGSNVRNRVIA CCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH RLIETAELAVSIEAWIKALRLNEPFCETSREAADGNYVGLVEAARGSLGHWLAVRSGRIA HHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEECCCCCCHHEEEECCCCEE RYQIIAPTSWNFSPRDARGVAGPLEQALVGTEVGEAGARAVSIQHVVRSFDPCMVCTAH EEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECC >Mature Secondary Structure TRITVGPFNRVEGDLEVRLDIESGRVARAEVTAPLYRGFEQILEGRPPLDALVIAPRIC CEEECCCHHHCCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH GICSVSQSIAAAAALRQAMGVVVAPNGVLATNIAHAAENAADHLTHFYIFFMPDFAREAY HHHHHHHHHHHHHHHHHHCCEEECCCCEEHHHHHHHHHHHHHHHHEEEEEECCHHHHHHH ASHSWHGAAAERFTATKGSAAREALPARARLLEIMGVIAGKWPHSLAFQPGGTTRAIELG HCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHC ERVRLNAIVASFRGFLERVVFADKLEHVLALSTPDELDRWRDGRSGDFAQFLRIADALDL CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHH AYLGAGPGLLMSFGAYRDADGARFRAGLRTPDGKIEALPSGQITEDVSHAWMRETSRDPA HHHCCCCHHHHHHCCCCCCCCCCEECCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCC ESRTEPDADKPDAYSWCKAPRLAGEPVEVGALARQAVHGQQLITALLSESGSNVRNRVIA CCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH RLIETAELAVSIEAWIKALRLNEPFCETSREAADGNYVGLVEAARGSLGHWLAVRSGRIA HHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEECCCCCCHHEEEECCCCEE RYQIIAPTSWNFSPRDARGVAGPLEQALVGTEVGEAGARAVSIQHVVRSFDPCMVCTAH EEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 3316183; 10378275 [H]