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The map label for this gene is hupU [C]

Identifier: 146338748

GI number: 146338748

Start: 1799141

End: 1800157

Strand: Direct

Name: hupU [C]

Synonym: BRADO1682

Alternate gene names: 146338748

Gene position: 1799141-1800157 (Clockwise)

Preceding gene: 146338747

Following gene: 146338749

Centisome position: 24.13

GC content: 67.65

Gene sequence:

>1017_bases
ATGGCCGGCGCGGAGGGCGGGATCAGGATGCTGTGGTTGCAGGGCGCGAGTTGCGGCGGCTGCACCATGTCCATCCTGGA
GAGCGGCGCGTCCGGCTGGTTCGACGAACTGCGGCAATCGGGCATCGACGTGATCTGGCATCCCTCGGTCAGCGAGCAGA
CCGGTGACGAGGTGGTCGACCTGCTCGAAGCGATCCGCGACGGCCGCGAGCAACTGGATCTCCTGGTGCTCGAAGGCTCT
GTCGCACGCGGGCCGCGTCTGAGCGGCCGCTTCAACATGCTCGCCGGCACCAACCGCTCGATCTACCATTGGCTGCTCGA
TCTCGCGCCGCTTGCCAACTACGTCGTCGCGGTCGGGAGTTGTGCCGCCTATGGCGGCATTCCCGCGGCCGGGATCAATC
CGACCGATGCCGTCGGCTTGCAGTTCGAGGGCAGCGATGCGGGCGGTGCGCTGGGCGCGGGGTTCCGATCGAAGCGCGGG
CTACCTGTGATCAATGTCGCCGGCTGCGCGCCGCATCCGGGCTGGATGATGGAAACGTTGCTGGCGCTCACAGCAGGCGA
TCTCACCGCCGATGGCCTCGACGCGGTGGGACGTCCGACTTTCATCGCCAATCACCTCGCGCATCACGGCTGCTCGCGCA
ACGAGTTCTACGAGTTCAAGGCGAGCGCGGAAACGATGTCGGAGCGCGGCTGCCTGATGGAGCATCTCGGCTGCCGCGCG
ACCCAGGCCGTCGGCGACTGCAATCAGCGTTCGTGGAACGGCGGCGGCTCCTGCACCAAGGGCGGCTATGCCTGCATCGC
CTGCACGTCGCCGGGCTTCGAGAGCCAGCAGAACTATCTGCAGACGGCGAAGCTCGCGGGCATTCCCGTGGGCCTCCCCA
CCGACATGCCCAAGGCCTGGTTCGTCGCGTTGGCCGCGCTGTCGAAATCGGCGACACCGCGCCGCGTCCGCGTCAACGCG
ACCGCCGATCATGTCGTGGTGCCGCCGAGCCGGTCCGGCGACAAGCATCGCTCATGA

Upstream 100 bases:

>100_bases
CACCTCACATGCATCAGTCGAGGCGCTTGCCAGTGTTCGCGCCAGGACGCTAAACCCGACGGCGAAATAAGGCGGAACGC
ACGGGACGGAGCGACCCACG

Downstream 100 bases:

>100_bases
CGCGGATCACGGTCGGCCCGTTCAATCGCGTCGAGGGGGATCTCGAGGTCCGTCTCGACATCGAGAGCGGCCGCGTCGCG
CGCGCCGAGGTGACGGCGCC

Product: uptake hydrogenase accessory protein HupU

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MAGAEGGIRMLWLQGASCGGCTMSILESGASGWFDELRQSGIDVIWHPSVSEQTGDEVVDLLEAIRDGREQLDLLVLEGS
VARGPRLSGRFNMLAGTNRSIYHWLLDLAPLANYVVAVGSCAAYGGIPAAGINPTDAVGLQFEGSDAGGALGAGFRSKRG
LPVINVAGCAPHPGWMMETLLALTAGDLTADGLDAVGRPTFIANHLAHHGCSRNEFYEFKASAETMSERGCLMEHLGCRA
TQAVGDCNQRSWNGGGSCTKGGYACIACTSPGFESQQNYLQTAKLAGIPVGLPTDMPKAWFVALAALSKSATPRRVRVNA
TADHVVVPPSRSGDKHRS

Sequences:

>Translated_338_residues
MAGAEGGIRMLWLQGASCGGCTMSILESGASGWFDELRQSGIDVIWHPSVSEQTGDEVVDLLEAIRDGREQLDLLVLEGS
VARGPRLSGRFNMLAGTNRSIYHWLLDLAPLANYVVAVGSCAAYGGIPAAGINPTDAVGLQFEGSDAGGALGAGFRSKRG
LPVINVAGCAPHPGWMMETLLALTAGDLTADGLDAVGRPTFIANHLAHHGCSRNEFYEFKASAETMSERGCLMEHLGCRA
TQAVGDCNQRSWNGGGSCTKGGYACIACTSPGFESQQNYLQTAKLAGIPVGLPTDMPKAWFVALAALSKSATPRRVRVNA
TADHVVVPPSRSGDKHRS
>Mature_337_residues
AGAEGGIRMLWLQGASCGGCTMSILESGASGWFDELRQSGIDVIWHPSVSEQTGDEVVDLLEAIRDGREQLDLLVLEGSV
ARGPRLSGRFNMLAGTNRSIYHWLLDLAPLANYVVAVGSCAAYGGIPAAGINPTDAVGLQFEGSDAGGALGAGFRSKRGL
PVINVAGCAPHPGWMMETLLALTAGDLTADGLDAVGRPTFIANHLAHHGCSRNEFYEFKASAETMSERGCLMEHLGCRAT
QAVGDCNQRSWNGGGSCTKGGYACIACTSPGFESQQNYLQTAKLAGIPVGLPTDMPKAWFVALAALSKSATPRRVRVNAT
ADHVVVPPSRSGDKHRS

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=284, Percent_Identity=28.169014084507, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787206, Length=287, Percent_Identity=25.0871080139373, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 35474; Mature: 35342

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGAEGGIRMLWLQGASCGGCTMSILESGASGWFDELRQSGIDVIWHPSVSEQTGDEVVD
CCCCCCCEEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHH
LLEAIRDGREQLDLLVLEGSVARGPRLSGRFNMLAGTNRSIYHWLLDLAPLANYVVAVGS
HHHHHHCCHHHEEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
CAAYGGIPAAGINPTDAVGLQFEGSDAGGALGAGFRSKRGLPVINVAGCAPHPGWMMETL
HHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHH
LALTAGDLTADGLDAVGRPTFIANHLAHHGCSRNEFYEFKASAETMSERGCLMEHLGCRA
HHHHCCCCCCCCHHHCCCCHHHHHHHHHCCCCCCHHEEEHHHHHHHHHCCHHHHHHCCHH
TQAVGDCNQRSWNGGGSCTKGGYACIACTSPGFESQQNYLQTAKLAGIPVGLPTDMPKAW
HHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHH
FVALAALSKSATPRRVRVNATADHVVVPPSRSGDKHRS
HHHHHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
AGAEGGIRMLWLQGASCGGCTMSILESGASGWFDELRQSGIDVIWHPSVSEQTGDEVVD
CCCCCCEEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHH
LLEAIRDGREQLDLLVLEGSVARGPRLSGRFNMLAGTNRSIYHWLLDLAPLANYVVAVGS
HHHHHHCCHHHEEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
CAAYGGIPAAGINPTDAVGLQFEGSDAGGALGAGFRSKRGLPVINVAGCAPHPGWMMETL
HHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHH
LALTAGDLTADGLDAVGRPTFIANHLAHHGCSRNEFYEFKASAETMSERGCLMEHLGCRA
HHHHCCCCCCCCHHHCCCCHHHHHHHHHCCCCCCHHEEEHHHHHHHHHCCHHHHHHCCHH
TQAVGDCNQRSWNGGGSCTKGGYACIACTSPGFESQQNYLQTAKLAGIPVGLPTDMPKAW
HHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHH
FVALAALSKSATPRRVRVNATADHVVVPPSRSGDKHRS
HHHHHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]