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The map label for this gene is intA [H]

Identifier: 146338350

GI number: 146338350

Start: 1345830

End: 1347104

Strand: Reverse

Name: intA [H]

Synonym: BRADO1258

Alternate gene names: 146338350

Gene position: 1347104-1345830 (Counterclockwise)

Preceding gene: 146338351

Following gene: 146338344

Centisome position: 18.07

GC content: 61.1

Gene sequence:

>1275_bases
ATGGCGAATTTGGGTATTACGGTTCGAACGGTGCAATCGCTCGGTCCTGGTGAAGCGATTTGGGATGCGGATCACAAAGA
GGCGGTGCGGGGATTTGGCGTGCGACGTCAACGGGGTACGCCCGTCTATGTCCTGAAGTTTCGCGCGTCCGGCCGACAGC
GCTTCTTTACCATTGGTCCCCATGGGTCCCCGTGGACCCCTGAAAAGGCGCGGAAGGAAGCAAAGCGCCTGCTTGGGCTG
GTTGCTCAGGGAAAGGATCCGGCTGCCGAACGTGAGCTCTTGACGTTACAGACGGCGGACACGTTCCGAAAGGTTGCCGA
CGAGTACCTAAAGACCGCCAAGCAAAAGCAGCGCCCCCGGACTTACTCGGAAATCGAACGGTATCTGCTCGGCGTGTGGA
AACCTCTGCACCAGCTCCCGATGTCGCAAATCAGCCGTCGCCATGTTGCCGCTCGTGTCGGCGATATCGCGACGAACAAT
GGGGCGGTCACGGCGGCTCGCGCGCGCTCGGCATTGTCAGCCATGTTCAACTGGGCCATTCGAGAGGGCCTTGAGATTCC
GGCGAACCCAGTGCTCGGGACCAACCGCCCGGCCGCACCAAGATCGAGGGAGCGGGTCCTAACCGATGGAGAACTGGCCG
AGATCTGGACAGGGCTCGAGGACGACGATTACGGACGCATCGTCAAGCTGCTCATCTTGACTGCCCAGCGTAGAGACGAA
GTTGGCGGAATGCGTTGGGCCGAAATCGACGGCTCAAGGTGTCTCTGGACCATCCCCGGTTCCCGAACGAAAAACCATCG
CGAGCACGCACTTCCACTGACAGAAGCGGCGATGCTGCTACTTCCCGCCGCCTCAGTCGGCAGAGAGTTCGTCTTCGGGG
ACGGACCGCGACGGAAGGGCGATGCACCGCGCGGCTTCTCAGGATGGTCGAAATCGAAGGCAGCGCTTGATGAGGGTCTA
CTAGCTAAGCGCAAGGAGAGGGCCGGTGTGGGGGAAACGGTCAATCCCATGCCCGAATGGTGGCTGCACGATCTTCGTCG
CACGGCCGCGACAGTGATGGCCGATCAGCTGGGAGTGCTGCCTCATATCATCGAAGCGGTGCTCAACCACGTCAGCGGCC
ATCGGGCGGGAGTGGCCGCCGTTTACAACCTTGCGCGATATGAAGCCGAAATGCGGGCTGCACTGACCGCATGGGCAAGC
CATGTCGACCGGCTGGTGCGAAGCAAGCCTTCGGTGGAGAAAGTTTCAGGCAGCTCGGCATGCCTCGATACGTGA

Upstream 100 bases:

>100_bases
TCAATAATTTCAATATCCCGCTCCGCCAAGTCCCGCCATACCTACGCTCCAATTTCCGCTACGGTGGTTGCTAGGTGGTA
GCTAGCAACCAAGGCTTCCG

Downstream 100 bases:

>100_bases
GCCGAATTCGGATCTCACGAACAATATCTGACCATGCTTGCCAGGCAAAGCCCGTCTCGCGGCCTCTTAGCCGCCTTCCG
CAAGATCCTAACGTTTGAAG

Product: putative phage-like integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MANLGITVRTVQSLGPGEAIWDADHKEAVRGFGVRRQRGTPVYVLKFRASGRQRFFTIGPHGSPWTPEKARKEAKRLLGL
VAQGKDPAAERELLTLQTADTFRKVADEYLKTAKQKQRPRTYSEIERYLLGVWKPLHQLPMSQISRRHVAARVGDIATNN
GAVTAARARSALSAMFNWAIREGLEIPANPVLGTNRPAAPRSRERVLTDGELAEIWTGLEDDDYGRIVKLLILTAQRRDE
VGGMRWAEIDGSRCLWTIPGSRTKNHREHALPLTEAAMLLLPAASVGREFVFGDGPRRKGDAPRGFSGWSKSKAALDEGL
LAKRKERAGVGETVNPMPEWWLHDLRRTAATVMADQLGVLPHIIEAVLNHVSGHRAGVAAVYNLARYEAEMRAALTAWAS
HVDRLVRSKPSVEKVSGSSACLDT

Sequences:

>Translated_424_residues
MANLGITVRTVQSLGPGEAIWDADHKEAVRGFGVRRQRGTPVYVLKFRASGRQRFFTIGPHGSPWTPEKARKEAKRLLGL
VAQGKDPAAERELLTLQTADTFRKVADEYLKTAKQKQRPRTYSEIERYLLGVWKPLHQLPMSQISRRHVAARVGDIATNN
GAVTAARARSALSAMFNWAIREGLEIPANPVLGTNRPAAPRSRERVLTDGELAEIWTGLEDDDYGRIVKLLILTAQRRDE
VGGMRWAEIDGSRCLWTIPGSRTKNHREHALPLTEAAMLLLPAASVGREFVFGDGPRRKGDAPRGFSGWSKSKAALDEGL
LAKRKERAGVGETVNPMPEWWLHDLRRTAATVMADQLGVLPHIIEAVLNHVSGHRAGVAAVYNLARYEAEMRAALTAWAS
HVDRLVRSKPSVEKVSGSSACLDT
>Mature_423_residues
ANLGITVRTVQSLGPGEAIWDADHKEAVRGFGVRRQRGTPVYVLKFRASGRQRFFTIGPHGSPWTPEKARKEAKRLLGLV
AQGKDPAAERELLTLQTADTFRKVADEYLKTAKQKQRPRTYSEIERYLLGVWKPLHQLPMSQISRRHVAARVGDIATNNG
AVTAARARSALSAMFNWAIREGLEIPANPVLGTNRPAAPRSRERVLTDGELAEIWTGLEDDDYGRIVKLLILTAQRRDEV
GGMRWAEIDGSRCLWTIPGSRTKNHREHALPLTEAAMLLLPAASVGREFVFGDGPRRKGDAPRGFSGWSKSKAALDEGLL
AKRKERAGVGETVNPMPEWWLHDLRRTAATVMADQLGVLPHIIEAVLNHVSGHRAGVAAVYNLARYEAEMRAALTAWASH
VDRLVRSKPSVEKVSGSSACLDT

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=174, Percent_Identity=32.7586206896552, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 46853; Mature: 46722

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLGITVRTVQSLGPGEAIWDADHKEAVRGFGVRRQRGTPVYVLKFRASGRQRFFTIGP
CCCCCEEHHHHHHCCCCCHHCCCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEECC
HGSPWTPEKARKEAKRLLGLVAQGKDPAAERELLTLQTADTFRKVADEYLKTAKQKQRPR
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCC
TYSEIERYLLGVWKPLHQLPMSQISRRHVAARVGDIATNNGAVTAARARSALSAMFNWAI
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
REGLEIPANPVLGTNRPAAPRSRERVLTDGELAEIWTGLEDDDYGRIVKLLILTAQRRDE
HCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VGGMRWAEIDGSRCLWTIPGSRTKNHREHALPLTEAAMLLLPAASVGREFVFGDGPRRKG
HCCCEEEEECCCEEEEECCCCCCCCCHHHCCCHHHHHHHHHCCHHHCCEEEECCCCCCCC
DAPRGFSGWSKSKAALDEGLLAKRKERAGVGETVNPMPEWWLHDLRRTAATVMADQLGVL
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
PHIIEAVLNHVSGHRAGVAAVYNLARYEAEMRAALTAWASHVDRLVRSKPSVEKVSGSSA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCC
CLDT
CCCC
>Mature Secondary Structure 
ANLGITVRTVQSLGPGEAIWDADHKEAVRGFGVRRQRGTPVYVLKFRASGRQRFFTIGP
CCCCEEHHHHHHCCCCCHHCCCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEECC
HGSPWTPEKARKEAKRLLGLVAQGKDPAAERELLTLQTADTFRKVADEYLKTAKQKQRPR
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCC
TYSEIERYLLGVWKPLHQLPMSQISRRHVAARVGDIATNNGAVTAARARSALSAMFNWAI
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
REGLEIPANPVLGTNRPAAPRSRERVLTDGELAEIWTGLEDDDYGRIVKLLILTAQRRDE
HCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VGGMRWAEIDGSRCLWTIPGSRTKNHREHALPLTEAAMLLLPAASVGREFVFGDGPRRKG
HCCCEEEEECCCEEEEECCCCCCCCCHHHCCCHHHHHHHHHCCHHHCCEEEECCCCCCCC
DAPRGFSGWSKSKAALDEGLLAKRKERAGVGETVNPMPEWWLHDLRRTAATVMADQLGVL
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
PHIIEAVLNHVSGHRAGVAAVYNLARYEAEMRAALTAWASHVDRLVRSKPSVEKVSGSSA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCC
CLDT
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]