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The map label for this gene is potI [H]

Identifier: 146338312

GI number: 146338312

Start: 1293072

End: 1293884

Strand: Direct

Name: potI [H]

Synonym: BRADO1217

Alternate gene names: 146338312

Gene position: 1293072-1293884 (Clockwise)

Preceding gene: 146338311

Following gene: 146338318

Centisome position: 17.34

GC content: 67.28

Gene sequence:

>813_bases
ATGCCTAAGCCCTCCCGCCTGTCCCGTTTCAACGTCGCCTCGCTGACGCTCGGGCTCGCCTTCCTGTATCTGCCGATCCT
GATCCTGGTGATCTACTCGTTCAACGCCTCGCGGCTGGTGACGGTGTGGGGCGGCTGGTCGCTGCGCTGGTATCATGAGT
TCTTCAACGACCGGGCGATGATCGACGCGGCCTGGATGAGCCTGGGCGTCGGCGCCACTTCGGCCACGTTGGCGACGGTG
CTCGGCACGCTGGCGGCGGTGGCGCTGTCGCGCGGCCAGGCATTCCGCGGCCGCATCCTGTTCTCCGGCATGCTGTATGC
GCCGCTAGTCATGCCCGAGGTGATCACCGGCCTGTCGCTGCTCCTGCTGTTCGTCGCACTGAGCGCCGAGCGCGGCTTCT
GGACGGTGACCATCGCGCACACGACGCTGACGATGTGCTTCGTCGCCGTGGTCGTGCAGTCGCGACTGGTGTCGCTCGAC
CGCAGCCTGGAGGAGGCGGCCATGGATCTCGGCTGCGATCCCTTCCGCGCCTTCGTCATGGTGACCTTGCCGCTGATCGC
GCCGGCGGTGGTGGCGGGGTGGATGCTGGCCTTCACGCTGTCACTCGACGATCTCGTGATCGCGAGCTTCACCACGGGGC
CGGGGTCGGCGACCTTGCCGATCCGGATCTATTCCGAGGTGAGGCTCGGGGTGAAGCCGGAGATCAACGCGATCTGCACG
CTGGTCATCGGGCTCATCGCGGTGGTCATCGTGGTCGCGTCCCTGGCGTCGAAGCTGTCGAGCGACCGCGGCGAGAGCGC
GGCGCCGCTTTAG

Upstream 100 bases:

>100_bases
TCGTCAAATGCGATAGCCCTGGAAGGGAGGGGCGTCCACAGCCGAGGCTGCTTAGAGTCTTACTTCAAGCTGCGGCCTGC
TTCGCAGGAGTTGGCCGCGC

Downstream 100 bases:

>100_bases
AAGCCATCATTGCGAGCGCAGCGAAGCAATCCAGAGTCCTTGGGCGGCCCTGGATTGCTTCGCTGCGCTCGCAATGACGG
AGATGTCCTGAGAGCGCCAC

Product: putrescine ABC transporter membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MPKPSRLSRFNVASLTLGLAFLYLPILILVIYSFNASRLVTVWGGWSLRWYHEFFNDRAMIDAAWMSLGVGATSATLATV
LGTLAAVALSRGQAFRGRILFSGMLYAPLVMPEVITGLSLLLLFVALSAERGFWTVTIAHTTLTMCFVAVVVQSRLVSLD
RSLEEAAMDLGCDPFRAFVMVTLPLIAPAVVAGWMLAFTLSLDDLVIASFTTGPGSATLPIRIYSEVRLGVKPEINAICT
LVIGLIAVVIVVASLASKLSSDRGESAAPL

Sequences:

>Translated_270_residues
MPKPSRLSRFNVASLTLGLAFLYLPILILVIYSFNASRLVTVWGGWSLRWYHEFFNDRAMIDAAWMSLGVGATSATLATV
LGTLAAVALSRGQAFRGRILFSGMLYAPLVMPEVITGLSLLLLFVALSAERGFWTVTIAHTTLTMCFVAVVVQSRLVSLD
RSLEEAAMDLGCDPFRAFVMVTLPLIAPAVVAGWMLAFTLSLDDLVIASFTTGPGSATLPIRIYSEVRLGVKPEINAICT
LVIGLIAVVIVVASLASKLSSDRGESAAPL
>Mature_269_residues
PKPSRLSRFNVASLTLGLAFLYLPILILVIYSFNASRLVTVWGGWSLRWYHEFFNDRAMIDAAWMSLGVGATSATLATVL
GTLAAVALSRGQAFRGRILFSGMLYAPLVMPEVITGLSLLLLFVALSAERGFWTVTIAHTTLTMCFVAVVVQSRLVSLDR
SLEEAAMDLGCDPFRAFVMVTLPLIAPAVVAGWMLAFTLSLDDLVIASFTTGPGSATLPIRIYSEVRLGVKPEINAICTL
VIGLIAVVIVVASLASKLSSDRGESAAPL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=256, Percent_Identity=55.46875, Blast_Score=250, Evalue=7e-68,
Organism=Escherichia coli, GI1787368, Length=237, Percent_Identity=42.1940928270042, Blast_Score=194, Evalue=4e-51,
Organism=Escherichia coli, GI1787714, Length=250, Percent_Identity=33.6, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28957; Mature: 28826

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKPSRLSRFNVASLTLGLAFLYLPILILVIYSFNASRLVTVWGGWSLRWYHEFFNDRAM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCHH
IDAAWMSLGVGATSATLATVLGTLAAVALSRGQAFRGRILFSGMLYAPLVMPEVITGLSL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLFVALSAERGFWTVTIAHTTLTMCFVAVVVQSRLVSLDRSLEEAAMDLGCDPFRAFVM
HHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VTLPLIAPAVVAGWMLAFTLSLDDLVIASFTTGPGSATLPIRIYSEVRLGVKPEINAICT
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEEECHHCCCCCCHHHHHH
LVIGLIAVVIVVASLASKLSSDRGESAAPL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PKPSRLSRFNVASLTLGLAFLYLPILILVIYSFNASRLVTVWGGWSLRWYHEFFNDRAM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCHH
IDAAWMSLGVGATSATLATVLGTLAAVALSRGQAFRGRILFSGMLYAPLVMPEVITGLSL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLFVALSAERGFWTVTIAHTTLTMCFVAVVVQSRLVSLDRSLEEAAMDLGCDPFRAFVM
HHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VTLPLIAPAVVAGWMLAFTLSLDDLVIASFTTGPGSATLPIRIYSEVRLGVKPEINAICT
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEEECHHCCCCCCHHHHHH
LVIGLIAVVIVVASLASKLSSDRGESAAPL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]