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The map label for this gene is potH [H]

Identifier: 146338311

GI number: 146338311

Start: 1291770

End: 1292702

Strand: Direct

Name: potH [H]

Synonym: BRADO1216

Alternate gene names: 146338311

Gene position: 1291770-1292702 (Clockwise)

Preceding gene: 146338310

Following gene: 146338312

Centisome position: 17.32

GC content: 63.88

Gene sequence:

>933_bases
TTGCGTGGTGCTGGAGCGATGAGCGCGCGCCGTATCTTCTCGCGACCGGCGCGCTTTGCTGCGATCGCCCCCTATGTCTG
GATGGTCCTGTTCTTCCTGGTGCCGTTCGCCTTCGTCCTCAAGATCAGCCTGTCGCAGACCGCGATAGCGCAGCCGCCCT
ACACGCCGGTCTTCTCGTGGGCGGACGGCTTGTCGTCACTGCGTGATGCCTTCGCGGCGCTGTCGTTCGACAATTTCCGG
CTGCTGCTGTCGGACGATCTATATCTCTCGTCCTATCTGCGCAGCGTCGTCGTCGCGCTGACGTCGACGGCGGTCTTGCT
GCTGATCGGCTATCCCATCGCCTACGGCATGGCGCGGCTGCCGAAGCGCTGGCAGGGCGTGGCGATGATGCTGGTCATCG
TGCCGTTCTGGACCTCGTTCCTGATCCGCATCTATGCCTGGGTCAACATCCTCCAGCATGACGGCCTGCTCAACCAGATC
CTGCTCGCGCTGCACATTGTGTCTGCGCCAGTCGTATGGCTGTCGACCGACAGCGCGATGTATGTCGGCATCGTCTACTC
CTATCTGCCGTTCATGATCCTGCCGCTGTTCGCGACGCTCACCAAGCTGGAGCCGGCGCTGGAGGAGGCGTCCGCCGATC
TCGGCGCGACGCCGCGCGAGACCTTCTGGCTGGTCACCTTCCCGCTGTCATTGCCCGGCGTTGGCGCCGGTGCGCTGTTG
TGCTTCATCCCGATCGTGGGCGAGTTCGTCATTCCCGATCTCCTCGCCGGCTCGAAGTCGCTGATGATCGGCCAGACCCT
GTGGCTTGAGTTCTTCACGAATAAGGACTGGCCGGTGGCCTCGAGCATCGCCGTGGTGCTGTTGGCCGTGCTGCTGTTGC
CGCTGCTGCTCTACGACCGGCTGCAGCGCCGGCAGTTGGAGGAGAGCCGGTGA

Upstream 100 bases:

>100_bases
GCGGCGCCGTGCTGCACGCCTCACAGGCCAACACGGCGCGGCTCGACGCCGACGGCTATGGTCTGAATCAGCGCGTCGTG
GCCTGGTTCACGCCCGATGA

Downstream 100 bases:

>100_bases
TGGAGGGTGCGCTCCCTATGCTCGCTCCGCCGCTCACCCGCTTCTGCAGGGCAGGGCTGTCACATTTGGAAATGCGGAGC
TGTCCCGCCGAACCATCCTG

Product: putrescine ABC transporter membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRGAGAMSARRIFSRPARFAAIAPYVWMVLFFLVPFAFVLKISLSQTAIAQPPYTPVFSWADGLSSLRDAFAALSFDNFR
LLLSDDLYLSSYLRSVVVALTSTAVLLLIGYPIAYGMARLPKRWQGVAMMLVIVPFWTSFLIRIYAWVNILQHDGLLNQI
LLALHIVSAPVVWLSTDSAMYVGIVYSYLPFMILPLFATLTKLEPALEEASADLGATPRETFWLVTFPLSLPGVGAGALL
CFIPIVGEFVIPDLLAGSKSLMIGQTLWLEFFTNKDWPVASSIAVVLLAVLLLPLLLYDRLQRRQLEESR

Sequences:

>Translated_310_residues
MRGAGAMSARRIFSRPARFAAIAPYVWMVLFFLVPFAFVLKISLSQTAIAQPPYTPVFSWADGLSSLRDAFAALSFDNFR
LLLSDDLYLSSYLRSVVVALTSTAVLLLIGYPIAYGMARLPKRWQGVAMMLVIVPFWTSFLIRIYAWVNILQHDGLLNQI
LLALHIVSAPVVWLSTDSAMYVGIVYSYLPFMILPLFATLTKLEPALEEASADLGATPRETFWLVTFPLSLPGVGAGALL
CFIPIVGEFVIPDLLAGSKSLMIGQTLWLEFFTNKDWPVASSIAVVLLAVLLLPLLLYDRLQRRQLEESR
>Mature_310_residues
MRGAGAMSARRIFSRPARFAAIAPYVWMVLFFLVPFAFVLKISLSQTAIAQPPYTPVFSWADGLSSLRDAFAALSFDNFR
LLLSDDLYLSSYLRSVVVALTSTAVLLLIGYPIAYGMARLPKRWQGVAMMLVIVPFWTSFLIRIYAWVNILQHDGLLNQI
LLALHIVSAPVVWLSTDSAMYVGIVYSYLPFMILPLFATLTKLEPALEEASADLGATPRETFWLVTFPLSLPGVGAGALL
CFIPIVGEFVIPDLLAGSKSLMIGQTLWLEFFTNKDWPVASSIAVVLLAVLLLPLLLYDRLQRRQLEESR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=269, Percent_Identity=47.2118959107807, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1787369, Length=284, Percent_Identity=36.6197183098592, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1787713, Length=251, Percent_Identity=30.6772908366534, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34455; Mature: 34455

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGAGAMSARRIFSRPARFAAIAPYVWMVLFFLVPFAFVLKISLSQTAIAQPPYTPVFSW
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ADGLSSLRDAFAALSFDNFRLLLSDDLYLSSYLRSVVVALTSTAVLLLIGYPIAYGMARL
HHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PKRWQGVAMMLVIVPFWTSFLIRIYAWVNILQHDGLLNQILLALHIVSAPVVWLSTDSAM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCHH
YVGIVYSYLPFMILPLFATLTKLEPALEEASADLGATPRETFWLVTFPLSLPGVGAGALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHH
CFIPIVGEFVIPDLLAGSKSLMIGQTLWLEFFTNKDWPVASSIAVVLLAVLLLPLLLYDR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LQRRQLEESR
HHHHHHHCCC
>Mature Secondary Structure
MRGAGAMSARRIFSRPARFAAIAPYVWMVLFFLVPFAFVLKISLSQTAIAQPPYTPVFSW
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ADGLSSLRDAFAALSFDNFRLLLSDDLYLSSYLRSVVVALTSTAVLLLIGYPIAYGMARL
HHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PKRWQGVAMMLVIVPFWTSFLIRIYAWVNILQHDGLLNQILLALHIVSAPVVWLSTDSAM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCHH
YVGIVYSYLPFMILPLFATLTKLEPALEEASADLGATPRETFWLVTFPLSLPGVGAGALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHH
CFIPIVGEFVIPDLLAGSKSLMIGQTLWLEFFTNKDWPVASSIAVVLLAVLLLPLLLYDR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LQRRQLEESR
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]