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The map label for this gene is lcfB [H]

Identifier: 146338035

GI number: 146338035

Start: 991001

End: 992536

Strand: Reverse

Name: lcfB [H]

Synonym: BRADO0928

Alternate gene names: 146338035

Gene position: 992536-991001 (Counterclockwise)

Preceding gene: 146338036

Following gene: 146338034

Centisome position: 13.31

GC content: 63.54

Gene sequence:

>1536_bases
ATGACCCACCCGTCCATTCATGCCCAGAGCCATCCGGACAAGATCGCCTATCAGATGGCGGGGACCGGCAAGGCCATCAC
CTATCGCGAGCTCGACGAGCTATCCAACCAGGGCGCCCAGCTGTTTCGCGCGCTCGGCCTCAAGGCCGGCGACCATGTCG
CGTTCCTGATGGAGAACCGGCTGGCCTTCATGGAGATCGCCTGGGCCGCGCAGCGCTCCGGCCTCTACTACACCGCGATC
AGCCGCTATCTCACCAAGGACGAAATCGCCTATATCGTCAGGGATTGCGGCGCCAAGGTCTTCATCACCTCGCCGAAATG
CGCCGAGCAGGTGCGCGATCTCGTCGCGGCGGACGGCCCGCTGTTCTTCATGGTCGACGAGCCCGAGCCCGGGTTTCGCT
CATGGGACAAGGAGGCCGGCGCGCAGCCCAAGACGCCGATCGCCGACCAGGTCGCCGGCTACGACATGCTGTATTCGTCG
GGCACCACCGGACGGCCGAAGGGAATCAAGAAGGATTTCGAAGGCAATCCGATCGATCAGCCCAACCAGTTCCTGCGCGT
GCTCTGCGCCGACATGTGCGGCATGAATGCCGAGAGCATCTATCTGTCGCCGGCGCCGCTGTATCATGCAGCGCCGCTGC
GCTTCAACATGATGGCGATCACGCTCGGCGGCACGTCGATCATCATGGAGCATTTCGACGCGGAGGAGTTCCTCAAGCTC
GTCGAGAAGTACAAGATCACGCAGTCGCAGCTGGTGCCGACGATGTTCGTGCGCATGCTAAAGCTGCCGGAGGACGTCCG
CCAGCGCTACGATGTTTCGACCCTGAAGGGCGCGATCCATGCGGCCGCGCCCTGCCCGGTCGACGTCAAGGCGAGGATGA
TCGAGTGGTGGGGGCCGATCTTGATCGAGTACTACGCGGGCTCGGAAGGCAATGGCGTCACGGTCTGCAACTCGAAGCAA
TGGCTGGAGCACCGCGGCAGCGTCGGCCGCGCTGTCGTCGGCAAGATCAAGATCCTCGGCGAGGATGATCAGGAAATGCC
GACCGGCGAGATCGGCACGGTCTATTTCGCCGATGCGCCTGTATTCTCCTATCACAACGATCCGGAGAAGACGAAGCGCG
CCTATAACGACAAGGGCTGGTCGACGCTCGGCGATGTCGGCTATCTCGACGCCGACGGCTTCCTCTATCTCACCGACCGC
AAGTCCTACATGATCATCTCCGGCGGCGTAAACATCTATCCGCAGGAGACCGAGGACGTCCTCATCACGCATCCGGATGT
CGCCGATGTCGCGGTGTTCGGCGTGCCGAACGAGGAGATGGGCGAGGAGGTCAAGGCCGTGGTGCAGCCGCACGACATGG
CGCGCGCCGGCAAGGCGCTCGAGGAGCAGCTGATCCTGTACTGCCGCAAGCACCTGTCGCCGCTGAAATGCCCGCGCTCG
ATCGACTTCGAGCCCGAGCTGCCGCGCACCCCGACCGGCAAGCTGGTCAAGCGCCATCTGCGCGAGCGCTACTGGCCGAA
GAAGGGCGCGGCGTAA

Upstream 100 bases:

>100_bases
CTGGAGCCACAGCTCGCTTTCACCACAGCCCCCTGTGGTTATGGGTCCCGGCTCGAGGCCGGGACGACGCCTTTTCCTGG
TTTAGCAGCGGTGAGCCCTC

Downstream 100 bases:

>100_bases
GCGGGCCGCGCCCGCAGCTCAAAGCAACGGTGTCGTCCCGGCCTTGAGCCGGGACCCATAGCCACCGTCCCGAGTTTTGC
GAAAGCTGGAGCTGCCAGCG

Product: long-chain-fatty-acid--CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MTHPSIHAQSHPDKIAYQMAGTGKAITYRELDELSNQGAQLFRALGLKAGDHVAFLMENRLAFMEIAWAAQRSGLYYTAI
SRYLTKDEIAYIVRDCGAKVFITSPKCAEQVRDLVAADGPLFFMVDEPEPGFRSWDKEAGAQPKTPIADQVAGYDMLYSS
GTTGRPKGIKKDFEGNPIDQPNQFLRVLCADMCGMNAESIYLSPAPLYHAAPLRFNMMAITLGGTSIIMEHFDAEEFLKL
VEKYKITQSQLVPTMFVRMLKLPEDVRQRYDVSTLKGAIHAAAPCPVDVKARMIEWWGPILIEYYAGSEGNGVTVCNSKQ
WLEHRGSVGRAVVGKIKILGEDDQEMPTGEIGTVYFADAPVFSYHNDPEKTKRAYNDKGWSTLGDVGYLDADGFLYLTDR
KSYMIISGGVNIYPQETEDVLITHPDVADVAVFGVPNEEMGEEVKAVVQPHDMARAGKALEEQLILYCRKHLSPLKCPRS
IDFEPELPRTPTGKLVKRHLRERYWPKKGAA

Sequences:

>Translated_511_residues
MTHPSIHAQSHPDKIAYQMAGTGKAITYRELDELSNQGAQLFRALGLKAGDHVAFLMENRLAFMEIAWAAQRSGLYYTAI
SRYLTKDEIAYIVRDCGAKVFITSPKCAEQVRDLVAADGPLFFMVDEPEPGFRSWDKEAGAQPKTPIADQVAGYDMLYSS
GTTGRPKGIKKDFEGNPIDQPNQFLRVLCADMCGMNAESIYLSPAPLYHAAPLRFNMMAITLGGTSIIMEHFDAEEFLKL
VEKYKITQSQLVPTMFVRMLKLPEDVRQRYDVSTLKGAIHAAAPCPVDVKARMIEWWGPILIEYYAGSEGNGVTVCNSKQ
WLEHRGSVGRAVVGKIKILGEDDQEMPTGEIGTVYFADAPVFSYHNDPEKTKRAYNDKGWSTLGDVGYLDADGFLYLTDR
KSYMIISGGVNIYPQETEDVLITHPDVADVAVFGVPNEEMGEEVKAVVQPHDMARAGKALEEQLILYCRKHLSPLKCPRS
IDFEPELPRTPTGKLVKRHLRERYWPKKGAA
>Mature_510_residues
THPSIHAQSHPDKIAYQMAGTGKAITYRELDELSNQGAQLFRALGLKAGDHVAFLMENRLAFMEIAWAAQRSGLYYTAIS
RYLTKDEIAYIVRDCGAKVFITSPKCAEQVRDLVAADGPLFFMVDEPEPGFRSWDKEAGAQPKTPIADQVAGYDMLYSSG
TTGRPKGIKKDFEGNPIDQPNQFLRVLCADMCGMNAESIYLSPAPLYHAAPLRFNMMAITLGGTSIIMEHFDAEEFLKLV
EKYKITQSQLVPTMFVRMLKLPEDVRQRYDVSTLKGAIHAAAPCPVDVKARMIEWWGPILIEYYAGSEGNGVTVCNSKQW
LEHRGSVGRAVVGKIKILGEDDQEMPTGEIGTVYFADAPVFSYHNDPEKTKRAYNDKGWSTLGDVGYLDADGFLYLTDRK
SYMIISGGVNIYPQETEDVLITHPDVADVAVFGVPNEEMGEEVKAVVQPHDMARAGKALEEQLILYCRKHLSPLKCPRSI
DFEPELPRTPTGKLVKRHLRERYWPKKGAA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=542, Percent_Identity=25.830258302583, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI38505220, Length=527, Percent_Identity=27.8937381404175, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI115511026, Length=521, Percent_Identity=24.9520153550864, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI157311624, Length=526, Percent_Identity=25.0950570342205, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI157311622, Length=526, Percent_Identity=25.0950570342205, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI187761345, Length=531, Percent_Identity=25.4237288135593, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI187761343, Length=531, Percent_Identity=25.4237288135593, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI58082049, Length=526, Percent_Identity=25.4752851711027, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI122937307, Length=525, Percent_Identity=26.8571428571429, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI42544132, Length=506, Percent_Identity=25.4940711462451, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI40807357, Length=550, Percent_Identity=25.4545454545455, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI38524616, Length=549, Percent_Identity=25.3187613843352, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI28416953, Length=566, Percent_Identity=24.0282685512367, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI62865631, Length=546, Percent_Identity=23.6263736263736, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI13325055, Length=546, Percent_Identity=23.6263736263736, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI13375727, Length=541, Percent_Identity=21.996303142329, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788107, Length=531, Percent_Identity=28.060263653484, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI221142682, Length=514, Percent_Identity=26.2645914396887, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI145693145, Length=518, Percent_Identity=28.957528957529, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1786810, Length=513, Percent_Identity=23.1968810916179, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790505, Length=559, Percent_Identity=22.3613595706619, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786529, Length=529, Percent_Identity=21.9281663516068, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=494, Percent_Identity=28.7449392712551, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17531443, Length=533, Percent_Identity=28.1425891181989, Blast_Score=168, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI32563687, Length=502, Percent_Identity=27.4900398406374, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17559526, Length=522, Percent_Identity=27.7777777777778, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71994694, Length=538, Percent_Identity=26.9516728624535, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71994690, Length=538, Percent_Identity=26.9516728624535, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71994703, Length=538, Percent_Identity=26.9516728624535, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17557194, Length=529, Percent_Identity=27.7882797731569, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=514, Percent_Identity=24.7081712062257, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=525, Percent_Identity=26.6666666666667, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17551278, Length=555, Percent_Identity=24.5045045045045, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=493, Percent_Identity=23.5294117647059, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI115534440, Length=561, Percent_Identity=25.1336898395722, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=503, Percent_Identity=21.6699801192843, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=377, Percent_Identity=22.8116710875332, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=538, Percent_Identity=24.5353159851301, Blast_Score=120, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6319264, Length=558, Percent_Identity=21.6845878136201, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648260, Length=518, Percent_Identity=27.027027027027, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI21356441, Length=513, Percent_Identity=25.3411306042885, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24648253, Length=469, Percent_Identity=25.5863539445629, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24648255, Length=469, Percent_Identity=25.5863539445629, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21355181, Length=489, Percent_Identity=25.7668711656442, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24581924, Length=532, Percent_Identity=24.812030075188, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19922652, Length=515, Percent_Identity=25.0485436893204, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI18859661, Length=508, Percent_Identity=25.1968503937008, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21358303, Length=474, Percent_Identity=25.3164556962025, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24648257, Length=519, Percent_Identity=23.5067437379576, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI281365686, Length=323, Percent_Identity=26.3157894736842, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356947, Length=512, Percent_Identity=22.8515625, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24656500, Length=511, Percent_Identity=23.091976516634, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161076582, Length=510, Percent_Identity=21.9607843137255, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI20130357, Length=498, Percent_Identity=24.2971887550201, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24653035, Length=495, Percent_Identity=21.2121212121212, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24667955, Length=556, Percent_Identity=23.9208633093525, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21355225, Length=367, Percent_Identity=24.2506811989101, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI62472339, Length=554, Percent_Identity=23.4657039711191, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24667959, Length=370, Percent_Identity=25.4054054054054, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 57044; Mature: 56913

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPSIHAQSHPDKIAYQMAGTGKAITYRELDELSNQGAQLFRALGLKAGDHVAFLMENR
CCCCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHCC
LAFMEIAWAAQRSGLYYTAISRYLTKDEIAYIVRDCGAKVFITSPKCAEQVRDLVAADGP
HHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCC
LFFMVDEPEPGFRSWDKEAGAQPKTPIADQVAGYDMLYSSGTTGRPKGIKKDFEGNPIDQ
EEEEECCCCCCCCCCCHHCCCCCCCCCHHHHCCCHHEECCCCCCCCCCCCCCCCCCCCCC
PNQFLRVLCADMCGMNAESIYLSPAPLYHAAPLRFNMMAITLGGTSIIMEHFDAEEFLKL
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEEEECCHHHHHHHCCHHHHHHH
VEKYKITQSQLVPTMFVRMLKLPEDVRQRYDVSTLKGAIHAAAPCPVDVKARMIEWWGPI
HHHHHCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCE
LIEYYAGSEGNGVTVCNSKQWLEHRGSVGRAVVGKIKILGEDDQEMPTGEIGTVYFADAP
EEEEECCCCCCEEEEECCHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEECCC
VFSYHNDPEKTKRAYNDKGWSTLGDVGYLDADGFLYLTDRKSYMIISGGVNIYPQETEDV
EECCCCCHHHHHHHHCCCCCCCCCCCCEECCCCEEEEECCCCEEEEECCCEECCCCCCCE
LITHPDVADVAVFGVPNEEMGEEVKAVVQPHDMARAGKALEEQLILYCRKHLSPLKCPRS
EEECCCHHHEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IDFEPELPRTPTGKLVKRHLRERYWPKKGAA
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
THPSIHAQSHPDKIAYQMAGTGKAITYRELDELSNQGAQLFRALGLKAGDHVAFLMENR
CCCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHCC
LAFMEIAWAAQRSGLYYTAISRYLTKDEIAYIVRDCGAKVFITSPKCAEQVRDLVAADGP
HHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCC
LFFMVDEPEPGFRSWDKEAGAQPKTPIADQVAGYDMLYSSGTTGRPKGIKKDFEGNPIDQ
EEEEECCCCCCCCCCCHHCCCCCCCCCHHHHCCCHHEECCCCCCCCCCCCCCCCCCCCCC
PNQFLRVLCADMCGMNAESIYLSPAPLYHAAPLRFNMMAITLGGTSIIMEHFDAEEFLKL
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEEEECCHHHHHHHCCHHHHHHH
VEKYKITQSQLVPTMFVRMLKLPEDVRQRYDVSTLKGAIHAAAPCPVDVKARMIEWWGPI
HHHHHCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCE
LIEYYAGSEGNGVTVCNSKQWLEHRGSVGRAVVGKIKILGEDDQEMPTGEIGTVYFADAP
EEEEECCCCCCEEEEECCHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEECCC
VFSYHNDPEKTKRAYNDKGWSTLGDVGYLDADGFLYLTDRKSYMIISGGVNIYPQETEDV
EECCCCCHHHHHHHHCCCCCCCCCCCCEECCCCEEEEECCCCEEEEECCCEECCCCCCCE
LITHPDVADVAVFGVPNEEMGEEVKAVVQPHDMARAGKALEEQLILYCRKHLSPLKCPRS
EEECCCHHHEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IDFEPELPRTPTGKLVKRHLRERYWPKKGAA
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]