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The map label for this gene is b2999 [C]

Identifier: 146338002

GI number: 146338002

Start: 945898

End: 947142

Strand: Reverse

Name: b2999 [C]

Synonym: BRADO0895

Alternate gene names: 146338002

Gene position: 947142-945898 (Counterclockwise)

Preceding gene: 146338004

Following gene: 146338001

Centisome position: 12.7

GC content: 65.22

Gene sequence:

>1245_bases
ATGGCTGGCAAAAGCGCTGGACAGTGGATCGACATCGCCGCCGCCGATGGCGGCACGTTCAAGGGCTACCTCGCGGTGCC
CGCGTCGGGCTCCGGTCCCGGCATCGTGCTCCTGCAGGAAATCTTCGGCGTCAACGCATCGATGCGGGACGTTGCCGACT
ACTACGCCGAGGAAGGCTATGTCGTGCTCGCGCCGGACCTGTTCTGGCGCTTCGAGCCGGGTATCGAGCTCGGCTATGGC
GAGGCCGACTTCGGCAAGGCCTTCGGCTACTACCAGCGCTTCGACGTGAATCTCGCGATCAAGGACACGGCCGACGCGCT
GAAGGTGCTGCGCGCCCGTCCCGAATGCAAGGGCAAGGTCGGTGCGCTCGGCTTCTGCCTCGGCGGCAAGCTCGCCTACC
TCACCGCCGCCCGCACCGATGTCGATTGCGCCGTGTCCTATTATGGCGTCGGCATCGAGGCCGATATCGCGGAAGCCAAG
AACATCAAGGGCCCGATCGTCTTCCACTTCGCCGAGCTCGACAAGTTCGCGCCTGCCGAGGCGCGCGAGGCGATCAAGGC
CGGCTTTGCCGGCCGCGACGACGCCGAGTTCTATCTCTACCCCGGCTGTGATCACGCCTTCGCCGCGCCCGAGCGCGCCA
GCTTCGACAAGCCCGCGACCCTGATGGCGCATTCGCGCTCGATCGCCCTGTTCCGGAAGGTTCTGGGCCCGCATTACGAT
CTGTCGTCGTTGTGGGACAAGCACACCGAGCTCGAATTCGCCACCCGCTCGGCCGAGGCGACGATGGGCACGATGGTCGC
CGAGCCCTATGTCAACCACATCCCGACCATGACCGGCGGCGTCGGCTACAAGGACCTGCTGCGGTTCTACGCCAACCACT
TCATCCCGAAGACGCCCGCGGATACCAAGCTGGTGCCGATCTCGCGCACCATCGGCGCCGACCGCGTCGTCGACGAAATG
CTGTTCTGCTTCACCCACGACATCGAGATCGACTGGATGCTGCCGGGCATCCCGCCGACCGGCAAATATGTCGAGATCCC
GCTGGTCGCGATCGTGCGCTTCCGCGGCGACAAGCTCTACAACGAGCACATCTACTGGGATCAGGCCTCGGTGCTGGTGC
AGATCGGCCTGCTCGATCCGTCCAAGCTGCCGGTCGCAGGTGTCGAGACCGCCAAGAAGCTGGTCGATGAAAGCCTGCCC
TCGAACACGCTGATGAAGCGCTGGGCCGAGAGCGCGCCGCACTGA

Upstream 100 bases:

>100_bases
CTACTGTTTACATCCGACATTTTTACATTAAAAATTCAATGAAGAAAACACATGAGGCCCGGCGTCACTGTGGACGCTGG
CAGGACCGGGGAGCATGACG

Downstream 100 bases:

>100_bases
TCATCAGGCGGGGATGGGGCCGGCAGGGGCGCCATTCCCCAATCTGTCATTTGCTCACTCATCGTTCGCTCATTTGGAGT
TGCATCACCATGCGTGACCA

Product: putative carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MAGKSAGQWIDIAAADGGTFKGYLAVPASGSGPGIVLLQEIFGVNASMRDVADYYAEEGYVVLAPDLFWRFEPGIELGYG
EADFGKAFGYYQRFDVNLAIKDTADALKVLRARPECKGKVGALGFCLGGKLAYLTAARTDVDCAVSYYGVGIEADIAEAK
NIKGPIVFHFAELDKFAPAEAREAIKAGFAGRDDAEFYLYPGCDHAFAAPERASFDKPATLMAHSRSIALFRKVLGPHYD
LSSLWDKHTELEFATRSAEATMGTMVAEPYVNHIPTMTGGVGYKDLLRFYANHFIPKTPADTKLVPISRTIGADRVVDEM
LFCFTHDIEIDWMLPGIPPTGKYVEIPLVAIVRFRGDKLYNEHIYWDQASVLVQIGLLDPSKLPVAGVETAKKLVDESLP
SNTLMKRWAESAPH

Sequences:

>Translated_414_residues
MAGKSAGQWIDIAAADGGTFKGYLAVPASGSGPGIVLLQEIFGVNASMRDVADYYAEEGYVVLAPDLFWRFEPGIELGYG
EADFGKAFGYYQRFDVNLAIKDTADALKVLRARPECKGKVGALGFCLGGKLAYLTAARTDVDCAVSYYGVGIEADIAEAK
NIKGPIVFHFAELDKFAPAEAREAIKAGFAGRDDAEFYLYPGCDHAFAAPERASFDKPATLMAHSRSIALFRKVLGPHYD
LSSLWDKHTELEFATRSAEATMGTMVAEPYVNHIPTMTGGVGYKDLLRFYANHFIPKTPADTKLVPISRTIGADRVVDEM
LFCFTHDIEIDWMLPGIPPTGKYVEIPLVAIVRFRGDKLYNEHIYWDQASVLVQIGLLDPSKLPVAGVETAKKLVDESLP
SNTLMKRWAESAPH
>Mature_413_residues
AGKSAGQWIDIAAADGGTFKGYLAVPASGSGPGIVLLQEIFGVNASMRDVADYYAEEGYVVLAPDLFWRFEPGIELGYGE
ADFGKAFGYYQRFDVNLAIKDTADALKVLRARPECKGKVGALGFCLGGKLAYLTAARTDVDCAVSYYGVGIEADIAEAKN
IKGPIVFHFAELDKFAPAEAREAIKAGFAGRDDAEFYLYPGCDHAFAAPERASFDKPATLMAHSRSIALFRKVLGPHYDL
SSLWDKHTELEFATRSAEATMGTMVAEPYVNHIPTMTGGVGYKDLLRFYANHFIPKTPADTKLVPISRTIGADRVVDEML
FCFTHDIEIDWMLPGIPPTGKYVEIPLVAIVRFRGDKLYNEHIYWDQASVLVQIGLLDPSKLPVAGVETAKKLVDESLPS
NTLMKRWAESAPH

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 45282; Mature: 45151

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKSAGQWIDIAAADGGTFKGYLAVPASGSGPGIVLLQEIFGVNASMRDVADYYAEEGY
CCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCEEEEHHHHCCCCCHHHHHHHHHCCCE
VVLAPDLFWRFEPGIELGYGEADFGKAFGYYQRFDVNLAIKDTADALKVLRARPECKGKV
EEECCCEEEEECCCCEECCCCCHHHHHHCCEEEEEEEEEECCHHHHHHHHHCCCCCCCCC
GALGFCLGGKLAYLTAARTDVDCAVSYYGVGIEADIAEAKNIKGPIVFHFAELDKFAPAE
CEEEEECCCCEEEEEECCCCCCEEEEEECCCEEECHHHHCCCCCCEEEEEEHHHHCCCHH
AREAIKAGFAGRDDAEFYLYPGCDHAFAAPERASFDKPATLMAHSRSIALFRKVLGPHYD
HHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCC
LSSLWDKHTELEFATRSAEATMGTMVAEPYVNHIPTMTGGVGYKDLLRFYANHFIPKTPA
HHHHHHHHCCHHHHHCCHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCC
DTKLVPISRTIGADRVVDEMLFCFTHDIEIDWMLPGIPPTGKYVEIPLVAIVRFRGDKLY
CCEEEEEHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHEEEEEECCCEEE
NEHIYWDQASVLVQIGLLDPSKLPVAGVETAKKLVDESLPSNTLMKRWAESAPH
CCCEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
AGKSAGQWIDIAAADGGTFKGYLAVPASGSGPGIVLLQEIFGVNASMRDVADYYAEEGY
CCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCEEEEHHHHCCCCCHHHHHHHHHCCCE
VVLAPDLFWRFEPGIELGYGEADFGKAFGYYQRFDVNLAIKDTADALKVLRARPECKGKV
EEECCCEEEEECCCCEECCCCCHHHHHHCCEEEEEEEEEECCHHHHHHHHHCCCCCCCCC
GALGFCLGGKLAYLTAARTDVDCAVSYYGVGIEADIAEAKNIKGPIVFHFAELDKFAPAE
CEEEEECCCCEEEEEECCCCCCEEEEEECCCEEECHHHHCCCCCCEEEEEEHHHHCCCHH
AREAIKAGFAGRDDAEFYLYPGCDHAFAAPERASFDKPATLMAHSRSIALFRKVLGPHYD
HHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCC
LSSLWDKHTELEFATRSAEATMGTMVAEPYVNHIPTMTGGVGYKDLLRFYANHFIPKTPA
HHHHHHHHCCHHHHHCCHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCC
DTKLVPISRTIGADRVVDEMLFCFTHDIEIDWMLPGIPPTGKYVEIPLVAIVRFRGDKLY
CCEEEEEHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHEEEEEECCCEEE
NEHIYWDQASVLVQIGLLDPSKLPVAGVETAKKLVDESLPSNTLMKRWAESAPH
CCCEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8138139 [H]