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The map label for this gene is helA [H]

Identifier: 146338004

GI number: 146338004

Start: 947949

End: 949259

Strand: Reverse

Name: helA [H]

Synonym: BRADO0897

Alternate gene names: 146338004

Gene position: 949259-947949 (Counterclockwise)

Preceding gene: 146338006

Following gene: 146338002

Centisome position: 12.73

GC content: 68.65

Gene sequence:

>1311_bases
ATGGCGCCGTCGCAAGGCGACGGCACCATCATGCTGAAGCCGCGCAGCGAGTGGCCTGATCCGGCGAAGCCGAAAGCCGA
CCTCGTTGCCGAGATCGAGCGAGCCGCCGACGAGATCCCGGGCAGCAACTACGAGATCTCGCAACCTATTCAGCTGCGCT
TCAACGAGCTAATCTCCGGCGTGCGCAGCAATGTCGGCGTCAAGATCTTCGGCGACGATCTCGACATCCTGCAGGGCGCC
GCGCGCCAGGTCGAAGCCGCGATCCGCGGCATCGATGGCGCCACCGACATCAAGACCGAGCAGGTCGCCGGCCTGCCGAT
CCTGACCGTGAAGATCGACCGCCAGGCGCTGTCGCGCTACGGCCTCAGCATCGCCGAGGTTCAGAACGTGGTCGAGATCG
CGATCGGCGGCAAGGCTGTCGGCAAGCTGTTCGACGGCGACCGCCGCTTCGACATCGTCGTCCGCCTGCCCGAGCGTCTG
CGCGGCAATCTCGATACGATCCGCGCTATCCCGATCCCGCTGCCTGCGGCTGAGGACGCAAGCGCGGCGCCACTGCGCAC
TGCATTCAACGCCGCCGCGCAGCCGCGCTACGTGCCGCTGTCGTCGGTGGCCGCGGTCGCCTCGGCGCCTGGGCCGAACC
AGGTCAGCCGCGAGAACGGCAAGCGCCGCATCGTCGTGACGGCCAATGTGCGTGCCCGCGACCTCGGCTCGTTCGTCGCC
GAAACGCGGGCAGCAGTGGCCGAAAAGGTGAAGCTGCCGCCGGGCTATTGGATCGGCTGGGGCGGCCAGTTCGAGCAGCT
GCTCTCCGCCACCAAGCGGCTCACCATCGTGGTGCCGATCGCGCTGGCGCTGGTGTTCCTGCTGCTGTTCATGAGCATGG
GCTCGCTCGCGGATGCCGCGCTGGTGTTCTCCGGCGTGCCGCTGGCGCTGACCGGCGGCATCGCCGCGCTGCTGCTGCGC
GGCCTGCCGCTGTCGATCTCGGCGGGCGTCGGCTTCATCGCGCTGTCGGGCGTCGCCGTGCTCAACGGCCTCGTCATCAT
TGCCTTCATCGAACGGCTGCGCCAGGGGGGACGGAACATCATGGCCGCCGTTGAGGACGGCGCGCTGGCGCGACTACGGC
CGGTGCTGATGACGGCATTGGTCGCCTCCCTCGGCTTCGTGCCGATGGCACTCGCCACCGGCGCCGGCGCCGAGGTGCAG
CGCCCGCTCGCTACCGTCGTCATCGGCGGCATCATCTCCTCGACGATACTGACCCTGCTGGTGCTGCCAGCGCTCTACGT
CTTATTCAGGCGGGAGACGCGACCCGCCTGA

Upstream 100 bases:

>100_bases
GGTCGATCTGCAGAAGGCGCTCGAGGCGCGGCTGCGGCAGATCCCCGAAGTCAAGGACGTTTTCACCCGCATCGGCACCG
CCGAGGTCGCGACCGACCCG

Downstream 100 bases:

>100_bases
ACGATCTCACGACTGATGACGCTTGGTGATCGCCACCCGCGTCGCCTGGCCATAGAGCCCCGCCATCTGGTCGAGCTGCG
CGGTCGTCGCCGCGCAACTG

Product: multidrug ABC transporter transmembrane protein

Products: Proton [Cytoplasm]; silver [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MAPSQGDGTIMLKPRSEWPDPAKPKADLVAEIERAADEIPGSNYEISQPIQLRFNELISGVRSNVGVKIFGDDLDILQGA
ARQVEAAIRGIDGATDIKTEQVAGLPILTVKIDRQALSRYGLSIAEVQNVVEIAIGGKAVGKLFDGDRRFDIVVRLPERL
RGNLDTIRAIPIPLPAAEDASAAPLRTAFNAAAQPRYVPLSSVAAVASAPGPNQVSRENGKRRIVVTANVRARDLGSFVA
ETRAAVAEKVKLPPGYWIGWGGQFEQLLSATKRLTIVVPIALALVFLLLFMSMGSLADAALVFSGVPLALTGGIAALLLR
GLPLSISAGVGFIALSGVAVLNGLVIIAFIERLRQGGRNIMAAVEDGALARLRPVLMTALVASLGFVPMALATGAGAEVQ
RPLATVVIGGIISSTILTLLVLPALYVLFRRETRPA

Sequences:

>Translated_436_residues
MAPSQGDGTIMLKPRSEWPDPAKPKADLVAEIERAADEIPGSNYEISQPIQLRFNELISGVRSNVGVKIFGDDLDILQGA
ARQVEAAIRGIDGATDIKTEQVAGLPILTVKIDRQALSRYGLSIAEVQNVVEIAIGGKAVGKLFDGDRRFDIVVRLPERL
RGNLDTIRAIPIPLPAAEDASAAPLRTAFNAAAQPRYVPLSSVAAVASAPGPNQVSRENGKRRIVVTANVRARDLGSFVA
ETRAAVAEKVKLPPGYWIGWGGQFEQLLSATKRLTIVVPIALALVFLLLFMSMGSLADAALVFSGVPLALTGGIAALLLR
GLPLSISAGVGFIALSGVAVLNGLVIIAFIERLRQGGRNIMAAVEDGALARLRPVLMTALVASLGFVPMALATGAGAEVQ
RPLATVVIGGIISSTILTLLVLPALYVLFRRETRPA
>Mature_435_residues
APSQGDGTIMLKPRSEWPDPAKPKADLVAEIERAADEIPGSNYEISQPIQLRFNELISGVRSNVGVKIFGDDLDILQGAA
RQVEAAIRGIDGATDIKTEQVAGLPILTVKIDRQALSRYGLSIAEVQNVVEIAIGGKAVGKLFDGDRRFDIVVRLPERLR
GNLDTIRAIPIPLPAAEDASAAPLRTAFNAAAQPRYVPLSSVAAVASAPGPNQVSRENGKRRIVVTANVRARDLGSFVAE
TRAAVAEKVKLPPGYWIGWGGQFEQLLSATKRLTIVVPIALALVFLLLFMSMGSLADAALVFSGVPLALTGGIAALLLRG
LPLSISAGVGFIALSGVAVLNGLVIIAFIERLRQGGRNIMAAVEDGALARLRPVLMTALVASLGFVPMALATGAGAEVQR
PLATVVIGGIISSTILTLLVLPALYVLFRRETRPA

Specific function: Presumed to function with HelC and HelB in efflux of an unidentified substrate [H]

COG id: COG3696

COG function: function code P; Putative silver efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AcrB/AcrD/AcrF (TC 2.A.6) family [H]

Homologues:

Organism=Escherichia coli, GI1786788, Length=434, Percent_Identity=31.7972350230415, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1788391, Length=425, Percent_Identity=24, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788814, Length=420, Percent_Identity=25.7142857142857, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1788390, Length=432, Percent_Identity=28.2407407407407, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786667, Length=330, Percent_Identity=26.3636363636364, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789930, Length=428, Percent_Identity=23.1308411214953, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789666, Length=332, Percent_Identity=24.3975903614458, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001036
- InterPro:   IPR004763 [H]

Pfam domain/function: PF00873 ACR_tran [H]

EC number: NA

Molecular weight: Translated: 45909; Mature: 45778

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPSQGDGTIMLKPRSEWPDPAKPKADLVAEIERAADEIPGSNYEISQPIQLRFNELISG
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VRSNVGVKIFGDDLDILQGAARQVEAAIRGIDGATDIKTEQVAGLPILTVKIDRQALSRY
HHHCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEEECHHHHHHH
GLSIAEVQNVVEIAIGGKAVGKLFDGDRRFDIVVRLPERLRGNLDTIRAIPIPLPAAEDA
CCCHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCHHHCCCCCCEEEECCCCCCCCCC
SAAPLRTAFNAAAQPRYVPLSSVAAVASAPGPNQVSRENGKRRIVVTANVRARDLGSFVA
CCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHCCCCEEEEEEECCCHHHHHHHHH
ETRAAVAEKVKLPPGYWIGWGGQFEQLLSATKRLTIVVPIALALVFLLLFMSMGSLADAA
HHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHCHHHHHH
LVFSGVPLALTGGIAALLLRGLPLSISAGVGFIALSGVAVLNGLVIIAFIERLRQGGRNI
HHHCCCCHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
MAAVEDGALARLRPVLMTALVASLGFVPMALATGAGAEVQRPLATVVIGGIISSTILTLL
EEEECCCHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VLPALYVLFRRETRPA
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
APSQGDGTIMLKPRSEWPDPAKPKADLVAEIERAADEIPGSNYEISQPIQLRFNELISG
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VRSNVGVKIFGDDLDILQGAARQVEAAIRGIDGATDIKTEQVAGLPILTVKIDRQALSRY
HHHCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEEECHHHHHHH
GLSIAEVQNVVEIAIGGKAVGKLFDGDRRFDIVVRLPERLRGNLDTIRAIPIPLPAAEDA
CCCHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCHHHCCCCCCEEEECCCCCCCCCC
SAAPLRTAFNAAAQPRYVPLSSVAAVASAPGPNQVSRENGKRRIVVTANVRARDLGSFVA
CCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHCCCCEEEEEEECCCHHHHHHHHH
ETRAAVAEKVKLPPGYWIGWGGQFEQLLSATKRLTIVVPIALALVFLLLFMSMGSLADAA
HHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHCHHHHHH
LVFSGVPLALTGGIAALLLRGLPLSISAGVGFIALSGVAVLNGLVIIAFIERLRQGGRNI
HHHCCCCHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
MAAVEDGALARLRPVLMTALVASLGFVPMALATGAGAEVQRPLATVVIGGIISSTILTLL
EEEECCCHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VLPALYVLFRRETRPA
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; silver [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + silver [Cytoplasm] = Proton [Cytoplasm] + silver [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8613357 [H]