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The map label for this gene is asnB [H]

Identifier: 146337968

GI number: 146337968

Start: 911520

End: 913289

Strand: Direct

Name: asnB [H]

Synonym: BRADO0860

Alternate gene names: 146337968

Gene position: 911520-913289 (Clockwise)

Preceding gene: 146337967

Following gene: 146337969

Centisome position: 12.22

GC content: 64.92

Gene sequence:

>1770_bases
ATGTGCGGCATTTGTGGTGAAATCAGGCATTCCGGCCAGCCCGCGCTCGCGGCGCTCAGCCTGGTCAACGATGCGATGCA
GGCGCGGGGACCCGATGCGGCCGGACTCTATGTCCAGGGCGGGATCGCACTGGGACATCGGCGTCTGAGCATCCTCGATC
TCTCCTCCGCCGCGCAGCAGCCGATGATCGATGCCGCGCTCGGGCTCGGTATCGCATTCAACGGCTGCATCTATAACTTC
CGCGAGCTGCGCACCGAGCTGAAGGCGAAGGGCTATCGCTTCTTCTCCGACGGCGACACGGAAGTCATCCTCAAGGCCTA
TCACGCCTGGGGCAAGAACTGCGTGCAACGCTTCAAGGGCATGTTTGCTTTTGCGCTCTGGGAGCGCGACTGCGGGCGTG
TCGTGATCGCGCGCGACCGGCTCGGCATCAAGCCGCTCTATTACACCGAAGGTGACGGCTTCTTTCGATTCGCGTCGTCC
CTGCCGGCGCTGCTCGCCGGTGGCGGAGTCGACACCTCGCTGGATCTCACGGCGCTGCACCACTTCTTCAGCTTTCACGC
GGCCGTTCCCGCCCCGCGCACGATCCTCAACGGCGTGAAGAAGCTCGAGCCGGCGACGCTGCTGACCATCGAGCCGGACG
GCACGCGGCGTCGCGAGCGCTATTGGCACTTCAATGTCGGCGAGCAGCGCCCGACGGATCGGGCCATGACTGCCGATGAA
TGGTCGGAGGCGGTGCTGGATGCAATGACGGCGGCCGTCGACCGACGCCGGGTCGCCGACGTGCCCGTGGGCGTGCTGCT
GTCCGGCGGGCTCGATTCGTCGCTGCTGGTCGCACTGCTGTCCAAGCTCGGCCACCAGGACATCCGCACCTTCTCGATCG
GCTTCGATGCTGTGGGCGGGCACAGCGGCGATGAGTTTCGCTACTCGGACATCATCGCGCGCACGTTTGGTACAAATCAC
GAGCAGATCAGGATCGGCGGCCATCGCGCGCTCGAGGCGCTGCCGCATGCGATCGCCGCGATGTCGGAGCCGATGGTCAG
CCATGACGCGGTTGCGTTCCATCTCTTGTCCTCGGAGGTCGCCAAGCGGGTCAAGGTGGTGCAGAGCGGGCAGGGCGCCG
ACGAGGTGTTCGGCGGCTACAGCTGGTACCCCGGCTTCCTCTCGGCGAACGATCCGGTGCAGTCATATCAGAGCACCTAT
TTCGATTGGCAGCATGCGGACCTGCAGCGGCTGCTGTTGCCGGACATGCTCGGCGACGATGTCAGCCTCGCATTCGTCGA
GCGCTTCTTCGCCGAACACGGCGGTGCCGCGGTCGACAAGGTGCTGCAGATCGACGCCGAGATCATGCTGGTCGACGACC
CCGTGAAGCGGGTCGACAACATGACGATGGCGTTCGGACTGGAAGCGCGCGTGCCATTCCTCGATCACGACGTGGTCGAG
CTCGCCGCGCGCATCCCGGCCGAGCTGAAGGTAAAGAACGGCGGTAAGCATATCCTGAAGGAGGCCGCGCGACGCTGCGT
GCCGGCGGAGGTGATCGACCGTCCGAAAGGCTATTTCCCGGTGCCGTCATTGCGCTATCTGCGCGGGCCATTCCTCGACT
ATGTGCGTGACGTGCTGAGCGACGACAAGGCGCGCACGCGCGGTCTCTACCAACGTGATTTCGTCGACCACATGCTGCAG
AACCCGGAAGCCGAGATGAGCCCGAAGGGCCATTCGCGCCTCTGGCAGGCGGCTCTGCTGGAAGCCTGGCTGCAGACGCA
CGGCATTTGA

Upstream 100 bases:

>100_bases
TCCGCTCTTTGTCGCAGGCGCGATCGAGCTCGAGGCGGAACGCAAGAGATCACAGAGGGTTATCGGAGGCATGTAGAAGA
ACGGAGTGGGATGTTCCGCG

Downstream 100 bases:

>100_bases
ATCTGACAGGATTGATCATATGGGATTGGACCCGAAGATCCTCGAACGGCTGCGGCTCGGCCTGAGCGACGACGAGCGTC
GGCTCGTGATCCGCTCCGCC

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MCGICGEIRHSGQPALAALSLVNDAMQARGPDAAGLYVQGGIALGHRRLSILDLSSAAQQPMIDAALGLGIAFNGCIYNF
RELRTELKAKGYRFFSDGDTEVILKAYHAWGKNCVQRFKGMFAFALWERDCGRVVIARDRLGIKPLYYTEGDGFFRFASS
LPALLAGGGVDTSLDLTALHHFFSFHAAVPAPRTILNGVKKLEPATLLTIEPDGTRRRERYWHFNVGEQRPTDRAMTADE
WSEAVLDAMTAAVDRRRVADVPVGVLLSGGLDSSLLVALLSKLGHQDIRTFSIGFDAVGGHSGDEFRYSDIIARTFGTNH
EQIRIGGHRALEALPHAIAAMSEPMVSHDAVAFHLLSSEVAKRVKVVQSGQGADEVFGGYSWYPGFLSANDPVQSYQSTY
FDWQHADLQRLLLPDMLGDDVSLAFVERFFAEHGGAAVDKVLQIDAEIMLVDDPVKRVDNMTMAFGLEARVPFLDHDVVE
LAARIPAELKVKNGGKHILKEAARRCVPAEVIDRPKGYFPVPSLRYLRGPFLDYVRDVLSDDKARTRGLYQRDFVDHMLQ
NPEAEMSPKGHSRLWQAALLEAWLQTHGI

Sequences:

>Translated_589_residues
MCGICGEIRHSGQPALAALSLVNDAMQARGPDAAGLYVQGGIALGHRRLSILDLSSAAQQPMIDAALGLGIAFNGCIYNF
RELRTELKAKGYRFFSDGDTEVILKAYHAWGKNCVQRFKGMFAFALWERDCGRVVIARDRLGIKPLYYTEGDGFFRFASS
LPALLAGGGVDTSLDLTALHHFFSFHAAVPAPRTILNGVKKLEPATLLTIEPDGTRRRERYWHFNVGEQRPTDRAMTADE
WSEAVLDAMTAAVDRRRVADVPVGVLLSGGLDSSLLVALLSKLGHQDIRTFSIGFDAVGGHSGDEFRYSDIIARTFGTNH
EQIRIGGHRALEALPHAIAAMSEPMVSHDAVAFHLLSSEVAKRVKVVQSGQGADEVFGGYSWYPGFLSANDPVQSYQSTY
FDWQHADLQRLLLPDMLGDDVSLAFVERFFAEHGGAAVDKVLQIDAEIMLVDDPVKRVDNMTMAFGLEARVPFLDHDVVE
LAARIPAELKVKNGGKHILKEAARRCVPAEVIDRPKGYFPVPSLRYLRGPFLDYVRDVLSDDKARTRGLYQRDFVDHMLQ
NPEAEMSPKGHSRLWQAALLEAWLQTHGI
>Mature_589_residues
MCGICGEIRHSGQPALAALSLVNDAMQARGPDAAGLYVQGGIALGHRRLSILDLSSAAQQPMIDAALGLGIAFNGCIYNF
RELRTELKAKGYRFFSDGDTEVILKAYHAWGKNCVQRFKGMFAFALWERDCGRVVIARDRLGIKPLYYTEGDGFFRFASS
LPALLAGGGVDTSLDLTALHHFFSFHAAVPAPRTILNGVKKLEPATLLTIEPDGTRRRERYWHFNVGEQRPTDRAMTADE
WSEAVLDAMTAAVDRRRVADVPVGVLLSGGLDSSLLVALLSKLGHQDIRTFSIGFDAVGGHSGDEFRYSDIIARTFGTNH
EQIRIGGHRALEALPHAIAAMSEPMVSHDAVAFHLLSSEVAKRVKVVQSGQGADEVFGGYSWYPGFLSANDPVQSYQSTY
FDWQHADLQRLLLPDMLGDDVSLAFVERFFAEHGGAAVDKVLQIDAEIMLVDDPVKRVDNMTMAFGLEARVPFLDHDVVE
LAARIPAELKVKNGGKHILKEAARRCVPAEVIDRPKGYFPVPSLRYLRGPFLDYVRDVLSDDKARTRGLYQRDFVDHMLQ
NPEAEMSPKGHSRLWQAALLEAWLQTHGI

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=542, Percent_Identity=28.9667896678967, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25147557, Length=547, Percent_Identity=27.9707495429616, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI71993933, Length=547, Percent_Identity=27.9707495429616, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17560178, Length=401, Percent_Identity=27.6807980049875, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25147560, Length=337, Percent_Identity=29.3768545994065, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321563, Length=628, Percent_Identity=26.1146496815287, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325403, Length=570, Percent_Identity=26.8421052631579, Blast_Score=120, Evalue=5e-28,
Organism=Drosophila melanogaster, GI45553209, Length=551, Percent_Identity=26.8602540834846, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 65043; Mature: 65043

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGICGEIRHSGQPALAALSLVNDAMQARGPDAAGLYVQGGIALGHRRLSILDLSSAAQQ
CCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCEEECCCEEEEEECHHHHCC
PMIDAALGLGIAFNGCIYNFRELRTELKAKGYRFFSDGDTEVILKAYHAWGKNCVQRFKG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
MFAFALWERDCGRVVIARDRLGIKPLYYTEGDGFFRFASSLPALLAGGGVDTSLDLTALH
HHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHCHHHHCCCCCCCCHHHHHHH
HFFSFHAAVPAPRTILNGVKKLEPATLLTIEPDGTRRRERYWHFNVGEQRPTDRAMTADE
HHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHEEEECCCCCCCCCCCCCHHH
WSEAVLDAMTAAVDRRRVADVPVGVLLSGGLDSSLLVALLSKLGHQDIRTFSIGFDAVGG
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
HSGDEFRYSDIIARTFGTNHEQIRIGGHRALEALPHAIAAMSEPMVSHDAVAFHLLSSEV
CCCCCCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AKRVKVVQSGQGADEVFGGYSWYPGFLSANDPVQSYQSTYFDWQHADLQRLLLPDMLGDD
HHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
VSLAFVERFFAEHGGAAVDKVLQIDAEIMLVDDPVKRVDNMTMAFGLEARVPFLDHDVVE
HHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCHHHHHCCCHHEECCCCCCCCCCHHHHH
LAARIPAELKVKNGGKHILKEAARRCVPAEVIDRPKGYFPVPSLRYLRGPFLDYVRDVLS
HHHHCCCEEEECCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHC
DDKARTRGLYQRDFVDHMLQNPEAEMSPKGHSRLWQAALLEAWLQTHGI
CCHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGICGEIRHSGQPALAALSLVNDAMQARGPDAAGLYVQGGIALGHRRLSILDLSSAAQQ
CCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCEEECCCEEEEEECHHHHCC
PMIDAALGLGIAFNGCIYNFRELRTELKAKGYRFFSDGDTEVILKAYHAWGKNCVQRFKG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
MFAFALWERDCGRVVIARDRLGIKPLYYTEGDGFFRFASSLPALLAGGGVDTSLDLTALH
HHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHCHHHHCCCCCCCCHHHHHHH
HFFSFHAAVPAPRTILNGVKKLEPATLLTIEPDGTRRRERYWHFNVGEQRPTDRAMTADE
HHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHEEEECCCCCCCCCCCCCHHH
WSEAVLDAMTAAVDRRRVADVPVGVLLSGGLDSSLLVALLSKLGHQDIRTFSIGFDAVGG
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
HSGDEFRYSDIIARTFGTNHEQIRIGGHRALEALPHAIAAMSEPMVSHDAVAFHLLSSEV
CCCCCCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AKRVKVVQSGQGADEVFGGYSWYPGFLSANDPVQSYQSTYFDWQHADLQRLLLPDMLGDD
HHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
VSLAFVERFFAEHGGAAVDKVLQIDAEIMLVDDPVKRVDNMTMAFGLEARVPFLDHDVVE
HHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCHHHHHCCCHHEECCCCCCCCCCHHHHH
LAARIPAELKVKNGGKHILKEAARRCVPAEVIDRPKGYFPVPSLRYLRGPFLDYVRDVLS
HHHHCCCEEEECCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHC
DDKARTRGLYQRDFVDHMLQNPEAEMSPKGHSRLWQAALLEAWLQTHGI
CCHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]