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The map label for this gene is celE [H]

Identifier: 146302692

GI number: 146302692

Start: 6001626

End: 6002726

Strand: Reverse

Name: celE [H]

Synonym: Fjoh_4965

Alternate gene names: 146302692

Gene position: 6002726-6001626 (Counterclockwise)

Preceding gene: 146302694

Following gene: 146302691

Centisome position: 98.46

GC content: 32.43

Gene sequence:

>1101_bases
ATGTTTCTCAAAAAAAGAGCTCTTTTAATTTTGTTTTTGTTGAATTCAATTATTTCAACCTCACAAATTCAAAATTCAGC
AAAAACTTTTTTATACGAAGGCCGAATAGATAAACTTCAAAATGATAACGTCATTTTAATTGGAACTGCTTCTTCTGTAA
CTTTTAATTTTACAGGAAACGAATGTTCAATTTCACTTCAAAGCGTAGATTCTTATGAACATCATAATTATGTTCAGTTG
GTTTTGGATGGAAAATATATTGGAAAAATCAGAATTGAAAAAGGGGCAGCACAGTCATTTCCAATAAAAGTAACTTCAAA
TAAAAAAGAACATCGTTTAGAAATTTATAAAAATACTGAAGCGCACAGCGGTGGTATTTTATTTACCGGAACAACAGCAA
AACTGACTGCAATTTCTTTTAAAAAGAAAAAGAAAATCGAATTCATTGGAGATTCTATTACCTGCGGCGCCGCAAGTGAT
CCGTCTGATGTTCCTTGTGATAAAGGTGAATATTTAGATCATCATAATGGTTATTATGCTTATGGACCAACACTTTCAAG
AGCAATTGGAGCAGCATATTTAATGAGCTCAGTTTCGGGAATTGGAATGTACAGAAACTGGAATGATGAAAACAAAGATG
AAGTCATAATGCCGGACGCTTATCCGAATTTATATTTAACCAAAGATTCTTCAAAACCTAAATACGATTTTGCTTTTCAG
CCTGATATTATCAGTATTGCTTTAGGAACAAATGATTTTTCGGACGGAGACGGCAAAAAAGAACGTCTGCCTTTTAATGC
TTCTAAATATGTTTCAAATTACATCAATTTTATAAAAATGCTGTACGAGCATAATCCAAATGTGCAGATTGTCATTACAA
ACAGTCCAATGGTTAACGGCGAAAAAGGAGTAGTTTTTGAAGAGTGCCTCAAAAAAGTAAAAAATGCTTTTGCAGAAGAT
AAAAACCACAAAGCAATCCAGATTTTTAAATTTAAACCTATGACGCCAAAAGGCTGTACAGGTCATCCAGATGTTGCCGA
TCATAAAGTTCTGGCAGATGAATATGGACCATTTTTAAAAAAGCTTTTAAATGAAAAATAA

Upstream 100 bases:

>100_bases
ATGTGGTGTTTTTTTATTGCTTTTTGACGTTTTAGTATCTTTTTTGAAACAGTATTTGAAATATATTTGTGAATTGCATT
TATATTTTAACAAATAAATT

Downstream 100 bases:

>100_bases
TCTACTATTTGTTTTCTTTTTAATGATTTGTGTTTCAGTAAAGGCAAATATTACGCTTCCTAATATTTTTGGAGATAATA
TGGTTTTACAGCGCAATTCT

Product: GDSL family lipase

Products: NA

Alternate protein names: Cellulase E; Endo-1,4-beta-glucanase E; EgE [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MFLKKRALLILFLLNSIISTSQIQNSAKTFLYEGRIDKLQNDNVILIGTASSVTFNFTGNECSISLQSVDSYEHHNYVQL
VLDGKYIGKIRIEKGAAQSFPIKVTSNKKEHRLEIYKNTEAHSGGILFTGTTAKLTAISFKKKKKIEFIGDSITCGAASD
PSDVPCDKGEYLDHHNGYYAYGPTLSRAIGAAYLMSSVSGIGMYRNWNDENKDEVIMPDAYPNLYLTKDSSKPKYDFAFQ
PDIISIALGTNDFSDGDGKKERLPFNASKYVSNYINFIKMLYEHNPNVQIVITNSPMVNGEKGVVFEECLKKVKNAFAED
KNHKAIQIFKFKPMTPKGCTGHPDVADHKVLADEYGPFLKKLLNEK

Sequences:

>Translated_366_residues
MFLKKRALLILFLLNSIISTSQIQNSAKTFLYEGRIDKLQNDNVILIGTASSVTFNFTGNECSISLQSVDSYEHHNYVQL
VLDGKYIGKIRIEKGAAQSFPIKVTSNKKEHRLEIYKNTEAHSGGILFTGTTAKLTAISFKKKKKIEFIGDSITCGAASD
PSDVPCDKGEYLDHHNGYYAYGPTLSRAIGAAYLMSSVSGIGMYRNWNDENKDEVIMPDAYPNLYLTKDSSKPKYDFAFQ
PDIISIALGTNDFSDGDGKKERLPFNASKYVSNYINFIKMLYEHNPNVQIVITNSPMVNGEKGVVFEECLKKVKNAFAED
KNHKAIQIFKFKPMTPKGCTGHPDVADHKVLADEYGPFLKKLLNEK
>Mature_366_residues
MFLKKRALLILFLLNSIISTSQIQNSAKTFLYEGRIDKLQNDNVILIGTASSVTFNFTGNECSISLQSVDSYEHHNYVQL
VLDGKYIGKIRIEKGAAQSFPIKVTSNKKEHRLEIYKNTEAHSGGILFTGTTAKLTAISFKKKKKIEFIGDSITCGAASD
PSDVPCDKGEYLDHHNGYYAYGPTLSRAIGAAYLMSSVSGIGMYRNWNDENKDEVIMPDAYPNLYLTKDSSKPKYDFAFQ
PDIISIALGTNDFSDGDGKKERLPFNASKYVSNYINFIKMLYEHNPNVQIVITNSPMVNGEKGVVFEECLKKVKNAFAED
KNHKAIQIFKFKPMTPKGCTGHPDVADHKVLADEYGPFLKKLLNEK

Specific function: This enzyme catalyzes the endohydrolysis of 1,4-beta- glucosidic linkages in cellulose, lichenin and cereal beta-D- glucans [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 5 (cellulase A) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016134
- InterPro:   IPR002105
- InterPro:   IPR018242
- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001547
- InterPro:   IPR018087
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR001087 [H]

Pfam domain/function: PF00150 Cellulase; PF00404 Dockerin_1; PF00657 Lipase_GDSL [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 40950; Mature: 40950

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLKKRALLILFLLNSIISTSQIQNSAKTFLYEGRIDKLQNDNVILIGTASSVTFNFTGN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHCCCCCEEEEEECCEEEEEEECC
ECSISLQSVDSYEHHNYVQLVLDGKYIGKIRIEKGAAQSFPIKVTSNKKEHRLEIYKNTE
EEEEEEECCCCCCCCCEEEEEECCEEEEEEEEECCCCCCCCEEEECCCCCEEEEEEECCC
AHSGGILFTGTTAKLTAISFKKKKKIEFIGDSITCGAASDPSDVPCDKGEYLDHHNGYYA
CCCCCEEEECCCCEEEEEECCCCCCEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCEEE
YGPTLSRAIGAAYLMSSVSGIGMYRNWNDENKDEVIMPDAYPNLYLTKDSSKPKYDFAFQ
ECCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCCCCEEEEECCCCCCEEEEEC
PDIISIALGTNDFSDGDGKKERLPFNASKYVSNYINFIKMLYEHNPNVQIVITNSPMVNG
CCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
EKGVVFEECLKKVKNAFAEDKNHKAIQIFKFKPMTPKGCTGHPDVADHKVLADEYGPFLK
CCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KLLNEK
HHHCCC
>Mature Secondary Structure
MFLKKRALLILFLLNSIISTSQIQNSAKTFLYEGRIDKLQNDNVILIGTASSVTFNFTGN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHCCCCCEEEEEECCEEEEEEECC
ECSISLQSVDSYEHHNYVQLVLDGKYIGKIRIEKGAAQSFPIKVTSNKKEHRLEIYKNTE
EEEEEEECCCCCCCCCEEEEEECCEEEEEEEEECCCCCCCCEEEECCCCCEEEEEEECCC
AHSGGILFTGTTAKLTAISFKKKKKIEFIGDSITCGAASDPSDVPCDKGEYLDHHNGYYA
CCCCCEEEECCCCEEEEEECCCCCCEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCEEE
YGPTLSRAIGAAYLMSSVSGIGMYRNWNDENKDEVIMPDAYPNLYLTKDSSKPKYDFAFQ
ECCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCCCCEEEEECCCCCCEEEEEC
PDIISIALGTNDFSDGDGKKERLPFNASKYVSNYINFIKMLYEHNPNVQIVITNSPMVNG
CCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
EKGVVFEECLKKVKNAFAEDKNHKAIQIFKFKPMTPKGCTGHPDVADHKVLADEYGPFLK
CCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KLLNEK
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3066698 [H]