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The map label for this gene is nagB [C]

Identifier: 146302284

GI number: 146302284

Start: 5506632

End: 5508644

Strand: Reverse

Name: nagB [C]

Synonym: Fjoh_4557

Alternate gene names: 146302284

Gene position: 5508644-5506632 (Counterclockwise)

Preceding gene: 146302285

Following gene: 146302283

Centisome position: 90.35

GC content: 37.36

Gene sequence:

>2013_bases
ATGGTACAAGCAGTGAAAATTGCTTGTGCCATTTCAAAAACCAAAATTGTATATACCAAAAAAAGAAAAGAAATGAAAAG
TGCTTTAGAAATAAAACCTGATATCAGTTATAAGAGCGCCGGTAAATTTGAAGAAACCAGATTTGAGAAAATCCACAATG
AAATCTTCAAAAGTTCAGCTGAAGCTTCGGTAATTGTAGCACAGGAAATCGCTCAATTGATTAGATCTAAGCAGGAAAAG
AATAAATCTTGTGTGCTTGGACTTGCAACAGGCTCATCTCCAATAAAAGTATATGAAGAATTAGTGAGAATGCACAAAGA
AGAAGGACTGAGCTTTCATAATGTAATCACTTTCAACTTGGACGAATATTATCCAATGAGTAAAGAGAACAATCAGAGCT
ATCATTATTTCATGCATCAGCATCTTTTTAATCATATCGATATTAAACCTGAAAATGTTAATATTCCTGATGGAACAGTT
TCAATTGAAGAACTCAATCAATACTGCATCGACTATGAAATGAATATTAAACAAGCAGGCGGACTTGATTTTCAATTGCT
TGGAATTGGCCGTACAGGACACGTTGGTTTCAACGAGCCTGGATCGCACATTAATTCAGGAACCAGAATTATTACTCTGG
ATCATATTACAAGAGTAGACGCTTCATCTGACTTTAATGGAATCGACAACGTTCCAAAGAGAGCCATCACGATGGGAGTT
TCTACCATTATGAGATCAAAAAGAATTGTATTAATGGCTTGGGGACAAAATAAAGCAGATATCATCAAAAGAACCATTCA
GGGAGATATCAGCTCAGAAGTTCCGGCTACATTTCTGCAGAATCATCCAAACGCGACTTTCGTTCTGGATCAGTCTGCAG
CATCAGAATTAACACGCTTTAAAACACCTTGGCTGGTTGGAGAATGCATCTGGACACAGGAATTAAAAAGCAAAGCAATT
GTATGGTTATGCCAGAAAACCAAACAATCTATTTTAAAACTGACAGACCGTGATTACAACAATAACGGAATGTCTGATTT
ACTGGCACAGGAAGGTTCTGCTTATGATATGAACATCAATATGTTTAATATTCTGCAGCATACTATTACAGGCTGGCCGG
GAGGAAAACCAAACACAGATGATTCTCACCGTCCGGAAAGAGCCAATCCTGCTAAAAAACGAGTAATCCTGTTTAGTCCG
CATCCTGATGATGACGTGATTTCGATGGGAGGAACTTTTTCAAAATTGATAAAACAAGGCCACGATGTACATGTTGTATA
TCAAACCTCCGGAAATATTGCCGTAACTGACGATGAGGCATTGAAATTTGCAGAAGTATGCAACGATTTTGTTGGTGCTG
ATCTTCCAAAAGACATCAACTTCAAATCAGTAATTGAATTTTTGAATAACAAATCAGAAAATCAGATTGATTCTCTTGAA
GTTCGAAAACTAAAAGGATTAATCAGAAGAAGAGAATCTTACGCAGCAACAAGATACATTGGACTAAAAGATGAAAATAC
ACACTTTTTAGATCTTCCTTTTTATGAAACCGGACAGGTTAAAAAGAATCCGCTGGGGCAGGAGGATATCGAAATTGTAA
AAGAAATCATTGCCAGAATAAAACCGCATCAGGTTTTTGCAGCAGGAGATTTGGCAGATCCGCACGGAACACATGAAGTA
TGTCTGAATGCGATTTTTGCCGCAATGAAAGAGCTGAAACCAGAAAAATACATGGATGACTGCTGGTTATGGTTATACAG
AGGTGCATGGCACGAATGGGATATCCACGAAATTGATATGGCAGTGCCGTTAAGCCCTTCAGAAGTATTACTAAAACGCC
ATGCAATCTTATACCACCAGTCTCAAAAAGACAGAGTAATGTTCCAGGGAAATGATTCAAGAGAATTCTGGGTTAGAGCC
GAAGACCGAAATAAAAATACTGCAATTCTGTATGATGAATTAGGATTAGCAGAGTATGAAGCTATTGAAGCTTTTAAACG
TTTTGACTATTAA

Upstream 100 bases:

>100_bases
GACCAGATAATGGAGGAACCAGAGTTTGGTGGGATGTAAATGCTCCGAATTTCTAAAAAACAAAAATAAATAGATTACAG
ATTTTAAGTTTGGTTAGCAA

Downstream 100 bases:

>100_bases
AGTTGTTTTAAAACCGATTCGGGTGAGAGCGAATCAAATTTCAAAATTCAGTTTGACCTCGCTATAGGTGAGGTAAAGCA
GAAGTCATTCTGAGTTTTGT

Product: glucosamine-6-phosphate deaminase-like protein

Products: D-fructose 6-phosphate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MVQAVKIACAISKTKIVYTKKRKEMKSALEIKPDISYKSAGKFEETRFEKIHNEIFKSSAEASVIVAQEIAQLIRSKQEK
NKSCVLGLATGSSPIKVYEELVRMHKEEGLSFHNVITFNLDEYYPMSKENNQSYHYFMHQHLFNHIDIKPENVNIPDGTV
SIEELNQYCIDYEMNIKQAGGLDFQLLGIGRTGHVGFNEPGSHINSGTRIITLDHITRVDASSDFNGIDNVPKRAITMGV
STIMRSKRIVLMAWGQNKADIIKRTIQGDISSEVPATFLQNHPNATFVLDQSAASELTRFKTPWLVGECIWTQELKSKAI
VWLCQKTKQSILKLTDRDYNNNGMSDLLAQEGSAYDMNINMFNILQHTITGWPGGKPNTDDSHRPERANPAKKRVILFSP
HPDDDVISMGGTFSKLIKQGHDVHVVYQTSGNIAVTDDEALKFAEVCNDFVGADLPKDINFKSVIEFLNNKSENQIDSLE
VRKLKGLIRRRESYAATRYIGLKDENTHFLDLPFYETGQVKKNPLGQEDIEIVKEIIARIKPHQVFAAGDLADPHGTHEV
CLNAIFAAMKELKPEKYMDDCWLWLYRGAWHEWDIHEIDMAVPLSPSEVLLKRHAILYHQSQKDRVMFQGNDSREFWVRA
EDRNKNTAILYDELGLAEYEAIEAFKRFDY

Sequences:

>Translated_670_residues
MVQAVKIACAISKTKIVYTKKRKEMKSALEIKPDISYKSAGKFEETRFEKIHNEIFKSSAEASVIVAQEIAQLIRSKQEK
NKSCVLGLATGSSPIKVYEELVRMHKEEGLSFHNVITFNLDEYYPMSKENNQSYHYFMHQHLFNHIDIKPENVNIPDGTV
SIEELNQYCIDYEMNIKQAGGLDFQLLGIGRTGHVGFNEPGSHINSGTRIITLDHITRVDASSDFNGIDNVPKRAITMGV
STIMRSKRIVLMAWGQNKADIIKRTIQGDISSEVPATFLQNHPNATFVLDQSAASELTRFKTPWLVGECIWTQELKSKAI
VWLCQKTKQSILKLTDRDYNNNGMSDLLAQEGSAYDMNINMFNILQHTITGWPGGKPNTDDSHRPERANPAKKRVILFSP
HPDDDVISMGGTFSKLIKQGHDVHVVYQTSGNIAVTDDEALKFAEVCNDFVGADLPKDINFKSVIEFLNNKSENQIDSLE
VRKLKGLIRRRESYAATRYIGLKDENTHFLDLPFYETGQVKKNPLGQEDIEIVKEIIARIKPHQVFAAGDLADPHGTHEV
CLNAIFAAMKELKPEKYMDDCWLWLYRGAWHEWDIHEIDMAVPLSPSEVLLKRHAILYHQSQKDRVMFQGNDSREFWVRA
EDRNKNTAILYDELGLAEYEAIEAFKRFDY
>Mature_670_residues
MVQAVKIACAISKTKIVYTKKRKEMKSALEIKPDISYKSAGKFEETRFEKIHNEIFKSSAEASVIVAQEIAQLIRSKQEK
NKSCVLGLATGSSPIKVYEELVRMHKEEGLSFHNVITFNLDEYYPMSKENNQSYHYFMHQHLFNHIDIKPENVNIPDGTV
SIEELNQYCIDYEMNIKQAGGLDFQLLGIGRTGHVGFNEPGSHINSGTRIITLDHITRVDASSDFNGIDNVPKRAITMGV
STIMRSKRIVLMAWGQNKADIIKRTIQGDISSEVPATFLQNHPNATFVLDQSAASELTRFKTPWLVGECIWTQELKSKAI
VWLCQKTKQSILKLTDRDYNNNGMSDLLAQEGSAYDMNINMFNILQHTITGWPGGKPNTDDSHRPERANPAKKRVILFSP
HPDDDVISMGGTFSKLIKQGHDVHVVYQTSGNIAVTDDEALKFAEVCNDFVGADLPKDINFKSVIEFLNNKSENQIDSLE
VRKLKGLIRRRESYAATRYIGLKDENTHFLDLPFYETGQVKKNPLGQEDIEIVKEIIARIKPHQVFAAGDLADPHGTHEV
CLNAIFAAMKELKPEKYMDDCWLWLYRGAWHEWDIHEIDMAVPLSPSEVLLKRHAILYHQSQKDRVMFQGNDSREFWVRA
EDRNKNTAILYDELGLAEYEAIEAFKRFDY

Specific function: Catalyzes The Reversible Isomerization-Deamination Of Glucosamine 6-Phosphate (Glcn6p) To Form Fructose 6-Phosphate (Fru6p) And Ammonium Ion. [C]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923881, Length=232, Percent_Identity=38.3620689655172, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI13027378, Length=245, Percent_Identity=37.1428571428571, Blast_Score=161, Evalue=2e-39,
Organism=Escherichia coli, GI1786893, Length=222, Percent_Identity=38.7387387387387, Blast_Score=172, Evalue=9e-44,
Organism=Escherichia coli, GI1789530, Length=213, Percent_Identity=27.6995305164319, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI48994958, Length=215, Percent_Identity=26.9767441860465, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554876, Length=233, Percent_Identity=39.9141630901288, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24581960, Length=251, Percent_Identity=38.2470119521912, Blast_Score=173, Evalue=4e-43,
Organism=Drosophila melanogaster, GI19920764, Length=251, Percent_Identity=38.2470119521912, Blast_Score=173, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR003737
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso; PF02585 PIG-L [H]

EC number: 3.5.99.6

Molecular weight: Translated: 76363; Mature: 76363

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQAVKIACAISKTKIVYTKKRKEMKSALEIKPDISYKSAGKFEETRFEKIHNEIFKSSA
CCCEEEEEEEEECEEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
EASVIVAQEIAQLIRSKQEKNKSCVLGLATGSSPIKVYEELVRMHKEEGLSFHNVITFNL
CCEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEEEC
DEYYPMSKENNQSYHYFMHQHLFNHIDIKPENVNIPDGTVSIEELNQYCIDYEMNIKQAG
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHCCEEECCHHCC
GLDFQLLGIGRTGHVGFNEPGSHINSGTRIITLDHITRVDASSDFNGIDNVPKRAITMGV
CCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECEEEECCCCCCCCCCCCCHHHHHHHH
STIMRSKRIVLMAWGQNKADIIKRTIQGDISSEVPATFLQNHPNATFVLDQSAASELTRF
HHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHC
KTPWLVGECIWTQELKSKAIVWLCQKTKQSILKLTDRDYNNNGMSDLLAQEGSAYDMNIN
CCCCHHHHHHHHHHHHCHHEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEECCH
MFNILQHTITGWPGGKPNTDDSHRPERANPAKKRVILFSPHPDDDVISMGGTFSKLIKQG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEECCCHHHHHHHCC
HDVHVVYQTSGNIAVTDDEALKFAEVCNDFVGADLPKDINFKSVIEFLNNKSENQIDSLE
CCEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHH
VRKLKGLIRRRESYAATRYIGLKDENTHFLDLPFYETGQVKKNPLGQEDIEIVKEIIARI
HHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHC
KPHQVFAAGDLADPHGTHEVCLNAIFAAMKELKPEKYMDDCWLWLYRGAWHEWDIHEIDM
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEE
AVPLSPSEVLLKRHAILYHQSQKDRVMFQGNDSREFWVRAEDRNKNTAILYDELGLAEYE
ECCCCHHHHHHHHHHHHEECCCCCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCHHHH
AIEAFKRFDY
HHHHHHHCCC
>Mature Secondary Structure
MVQAVKIACAISKTKIVYTKKRKEMKSALEIKPDISYKSAGKFEETRFEKIHNEIFKSSA
CCCEEEEEEEEECEEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
EASVIVAQEIAQLIRSKQEKNKSCVLGLATGSSPIKVYEELVRMHKEEGLSFHNVITFNL
CCEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEEEC
DEYYPMSKENNQSYHYFMHQHLFNHIDIKPENVNIPDGTVSIEELNQYCIDYEMNIKQAG
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHCCEEECCHHCC
GLDFQLLGIGRTGHVGFNEPGSHINSGTRIITLDHITRVDASSDFNGIDNVPKRAITMGV
CCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECEEEECCCCCCCCCCCCCHHHHHHHH
STIMRSKRIVLMAWGQNKADIIKRTIQGDISSEVPATFLQNHPNATFVLDQSAASELTRF
HHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHC
KTPWLVGECIWTQELKSKAIVWLCQKTKQSILKLTDRDYNNNGMSDLLAQEGSAYDMNIN
CCCCHHHHHHHHHHHHCHHEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEECCH
MFNILQHTITGWPGGKPNTDDSHRPERANPAKKRVILFSPHPDDDVISMGGTFSKLIKQG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEECCCHHHHHHHCC
HDVHVVYQTSGNIAVTDDEALKFAEVCNDFVGADLPKDINFKSVIEFLNNKSENQIDSLE
CCEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHH
VRKLKGLIRRRESYAATRYIGLKDENTHFLDLPFYETGQVKKNPLGQEDIEIVKEIIARI
HHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHC
KPHQVFAAGDLADPHGTHEVCLNAIFAAMKELKPEKYMDDCWLWLYRGAWHEWDIHEIDM
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEE
AVPLSPSEVLLKRHAILYHQSQKDRVMFQGNDSREFWVRAEDRNKNTAILYDELGLAEYE
ECCCCHHHHHHHHHHHHEECCCCCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCHHHH
AIEAFKRFDY
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-glucosamine 6-phosphate; H2O

Specific reaction: D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3

General reaction: Group transfer (transmolecular Intramolecular oxidoreduction) [C]

Inhibitor: N3-(4-Methoxyfumaroyl)-L-2, 3-diaminopropanoic acid [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]