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The map label for this gene is nfdA [H]

Identifier: 146302010

GI number: 146302010

Start: 5163207

End: 5164994

Strand: Direct

Name: nfdA [H]

Synonym: Fjoh_4274

Alternate gene names: 146302010

Gene position: 5163207-5164994 (Clockwise)

Preceding gene: 146302009

Following gene: 146302011

Centisome position: 84.69

GC content: 37.02

Gene sequence:

>1788_bases
ATGAAACTCTCTTTTAAAATAGCAGCTTTCCTTTTAATTATCTCCCTTCCTATTTTCGGGCAAAGCAAAAAAGCAACTCT
AATCGTTCATCATGCTGTAATTCATACTTTAGACAATAAAAATACAATCGTTGAAGCCATGGCTGTCGCCGATGGAAGAA
TCCTAAAAACAGGGAAAAACAGCGAAATCTTAAAATTAAAAGACAAAAAAACAACCCTAATCGATGCTAAAGGGAAAACC
ATTATTCCCGGAATATTCGATTCTCATATGCACATCATCCGTGGAGGAAGATTTTACAATACCGAATTACGCTGGGACGG
CGTTCGTTCCTTAAAAAGAGCTTTGGCCATGCTGAAAGAACAAGCACAAAGAACTCCAAAAGGACAATGGGTTCGTGTGG
TAGGCGGATGGAATGCTTATCAGTTTGAAGAAAAAAGACTTCCTACCTTAGCCGAAATCAACGAAGCAACTGGCGATGTT
CCAGCTTTTATTCTTCATTTATATGGACACGCCTATTTGAACAAAGCAGGTTTAGAAACTTTGAAAATTGATGCCAATAC
ACCAAATCCAAATGCGGGATTGATTGAAAAAGATTCAAACGGAAATCCAACCGGATTGTTAGCTGCAGAACCAAATGCTT
TTATTTTATACTCTACCCTTGCCAAACTTCCAGAATTGACGCAAGAAGAAAAATTCAATTCGACCAAACAATTTATGACC
GAAATGAACCGTCTTGGCGTAACCGCTATTATGGATGCAGGAGGCGGATTCCAAAACTTTCCAGACGATTACGGCGTAAC
AAACGGTTTGTGCAAAGACAGCGATTTAACGATCAGAATGCCTTATTATTTGTTTGCTCAAAAAGCAGGAACAGAATTAA
ATGATTACACAAAATGGATGCAGACAGTCGAAATTGGCGAAGGCTGTGACGACGATCATCATTCAGATAAAGTAGAATAT
CACGTTCAGGGTGCAGGAGAAAATTTAGTTATGAGTGCCGGAGATTTTGAAAACTTCGACAAACCAAGACCAGAATTAAG
CCCAGCGATGGAAGGTCAGTTAAAAGAAGTTTTATCCTTATTAGTAAAAAACAGATGGCCGTTTAGAATTCACGCAACTT
ATAACGAAAGCATTACAAGATTTTTAAATGTAATTGAAGACATTAATAAAGAAACACCTTTAAACGGACTTTTATGGTTC
TTTGATCACGCCGAAACAGTTTCTACAGAAAATTTAAAACGTATTAAAGCTTTAAACGGCGGTATTGCAATTCAGCACAG
AATGGCGTATCAAGGTGAAAGTTTCATTAAAAGATATGGAAAAACAGCTGCTGCAAACACAGTTCCGCTAAAGAAAATGT
TCGAATTAGGAATTAAAGTAGGAATGGGAACAGACGGAACCCGTGTAGCAAGTTACAATCCGTGGGTTGGTTTATACTGG
CTTACAACCGGAAAAACTTTAGGAGGTTTAAAATACATGAACGACGAGAATATTGTAGACCGAACAACAGCTTTAAAATT
GTTCACTTACGGAAGCGCACAATTAATCAATATTGAAAAAGACAGAGGAATGCTGACTGCAGATAAACTAGCTGATTTCT
CAATCCTTTCTGATGATTATTTTACTACTTCTGAAGAAAATATCTTAAACATCGAATCAAAACTTACAGTTGTAAACGGT
AAAGCAGTTTATGCTGATAATGACTTTAGAACTTTTGTAAACGAAAAACCAAAAGCAATTCCAGACTGGAGTCCGGTAAA
TTACTTTGGAGGTTATCAAAAAAATTAA

Upstream 100 bases:

>100_bases
CAGTAAAATCCGCGGGCCAAAATATTTCCAGAAAAACAAAGAAAGGCTGTGTAACCGCACAGTCTTTTTTTTCTCCAAAA
CCAATTAAAATACCACAACC

Downstream 100 bases:

>100_bases
TTCAAAATGAAAAACACATTCTTCTTATTGTTGCTTTTGGGAATCTTTTTTTATGCAAACGCACAACAAAAACCAGTTTT
CCAAAAGCTGCGATATGAGG

Product: amidohydrolase 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MKLSFKIAAFLLIISLPIFGQSKKATLIVHHAVIHTLDNKNTIVEAMAVADGRILKTGKNSEILKLKDKKTTLIDAKGKT
IIPGIFDSHMHIIRGGRFYNTELRWDGVRSLKRALAMLKEQAQRTPKGQWVRVVGGWNAYQFEEKRLPTLAEINEATGDV
PAFILHLYGHAYLNKAGLETLKIDANTPNPNAGLIEKDSNGNPTGLLAAEPNAFILYSTLAKLPELTQEEKFNSTKQFMT
EMNRLGVTAIMDAGGGFQNFPDDYGVTNGLCKDSDLTIRMPYYLFAQKAGTELNDYTKWMQTVEIGEGCDDDHHSDKVEY
HVQGAGENLVMSAGDFENFDKPRPELSPAMEGQLKEVLSLLVKNRWPFRIHATYNESITRFLNVIEDINKETPLNGLLWF
FDHAETVSTENLKRIKALNGGIAIQHRMAYQGESFIKRYGKTAAANTVPLKKMFELGIKVGMGTDGTRVASYNPWVGLYW
LTTGKTLGGLKYMNDENIVDRTTALKLFTYGSAQLINIEKDRGMLTADKLADFSILSDDYFTTSEENILNIESKLTVVNG
KAVYADNDFRTFVNEKPKAIPDWSPVNYFGGYQKN

Sequences:

>Translated_595_residues
MKLSFKIAAFLLIISLPIFGQSKKATLIVHHAVIHTLDNKNTIVEAMAVADGRILKTGKNSEILKLKDKKTTLIDAKGKT
IIPGIFDSHMHIIRGGRFYNTELRWDGVRSLKRALAMLKEQAQRTPKGQWVRVVGGWNAYQFEEKRLPTLAEINEATGDV
PAFILHLYGHAYLNKAGLETLKIDANTPNPNAGLIEKDSNGNPTGLLAAEPNAFILYSTLAKLPELTQEEKFNSTKQFMT
EMNRLGVTAIMDAGGGFQNFPDDYGVTNGLCKDSDLTIRMPYYLFAQKAGTELNDYTKWMQTVEIGEGCDDDHHSDKVEY
HVQGAGENLVMSAGDFENFDKPRPELSPAMEGQLKEVLSLLVKNRWPFRIHATYNESITRFLNVIEDINKETPLNGLLWF
FDHAETVSTENLKRIKALNGGIAIQHRMAYQGESFIKRYGKTAAANTVPLKKMFELGIKVGMGTDGTRVASYNPWVGLYW
LTTGKTLGGLKYMNDENIVDRTTALKLFTYGSAQLINIEKDRGMLTADKLADFSILSDDYFTTSEENILNIESKLTVVNG
KAVYADNDFRTFVNEKPKAIPDWSPVNYFGGYQKN
>Mature_595_residues
MKLSFKIAAFLLIISLPIFGQSKKATLIVHHAVIHTLDNKNTIVEAMAVADGRILKTGKNSEILKLKDKKTTLIDAKGKT
IIPGIFDSHMHIIRGGRFYNTELRWDGVRSLKRALAMLKEQAQRTPKGQWVRVVGGWNAYQFEEKRLPTLAEINEATGDV
PAFILHLYGHAYLNKAGLETLKIDANTPNPNAGLIEKDSNGNPTGLLAAEPNAFILYSTLAKLPELTQEEKFNSTKQFMT
EMNRLGVTAIMDAGGGFQNFPDDYGVTNGLCKDSDLTIRMPYYLFAQKAGTELNDYTKWMQTVEIGEGCDDDHHSDKVEY
HVQGAGENLVMSAGDFENFDKPRPELSPAMEGQLKEVLSLLVKNRWPFRIHATYNESITRFLNVIEDINKETPLNGLLWF
FDHAETVSTENLKRIKALNGGIAIQHRMAYQGESFIKRYGKTAAANTVPLKKMFELGIKVGMGTDGTRVASYNPWVGLYW
LTTGKTLGGLKYMNDENIVDRTTALKLFTYGSAQLINIEKDRGMLTADKLADFSILSDDYFTTSEENILNIESKLTVVNG
KAVYADNDFRTFVNEKPKAIPDWSPVNYFGGYQKN

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 66610; Mature: 66610

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSFKIAAFLLIISLPIFGQSKKATLIVHHAVIHTLDNKNTIVEAMAVADGRILKTGKN
CCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEEEHEECCCCCHHHHHHHHCCCEEEECCCC
SEILKLKDKKTTLIDAKGKTIIPGIFDSHMHIIRGGRFYNTELRWDGVRSLKRALAMLKE
CCEEEEECCCEEEEECCCCEEECCHHCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHHH
QAQRTPKGQWVRVVGGWNAYQFEEKRLPTLAEINEATGDVPAFILHLYGHAYLNKAGLET
HHHHCCCCCEEEEEECCCCEEEHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEE
LKIDANTPNPNAGLIEKDSNGNPTGLLAAEPNAFILYSTLAKLPELTQEEKFNSTKQFMT
EEEECCCCCCCCCEEEECCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHCCHHHHHHH
EMNRLGVTAIMDAGGGFQNFPDDYGVTNGLCKDSDLTIRMPYYLFAQKAGTELNDYTKWM
HHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCHHEEHHHCCCCHHHHHHHH
QTVEIGEGCDDDHHSDKVEYHVQGAGENLVMSAGDFENFDKPRPELSPAMEGQLKEVLSL
HHHHCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
LVKNRWPFRIHATYNESITRFLNVIEDINKETPLNGLLWFFDHAETVSTENLKRIKALNG
HHCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCC
GIAIQHRMAYQGESFIKRYGKTAAANTVPLKKMFELGIKVGMGTDGTRVASYNPWVGLYW
CEEEEEEHHHCCHHHHHHHCCCCCCCCCCHHHHHHCCEEEECCCCCCEEEECCCCEEEEE
LTTGKTLGGLKYMNDENIVDRTTALKLFTYGSAQLINIEKDRGMLTADKLADFSILSDDY
EECCCCCCCEEECCCCCCHHHHEEEEEEECCCEEEEEEECCCCCEEHHHHCCEEEECCCC
FTTSEENILNIESKLTVVNGKAVYADNDFRTFVNEKPKAIPDWSPVNYFGGYQKN
CCCCCCCEEEEEEEEEEEECEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLSFKIAAFLLIISLPIFGQSKKATLIVHHAVIHTLDNKNTIVEAMAVADGRILKTGKN
CCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEEEHEECCCCCHHHHHHHHCCCEEEECCCC
SEILKLKDKKTTLIDAKGKTIIPGIFDSHMHIIRGGRFYNTELRWDGVRSLKRALAMLKE
CCEEEEECCCEEEEECCCCEEECCHHCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHHH
QAQRTPKGQWVRVVGGWNAYQFEEKRLPTLAEINEATGDVPAFILHLYGHAYLNKAGLET
HHHHCCCCCEEEEEECCCCEEEHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEE
LKIDANTPNPNAGLIEKDSNGNPTGLLAAEPNAFILYSTLAKLPELTQEEKFNSTKQFMT
EEEECCCCCCCCCEEEECCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHCCHHHHHHH
EMNRLGVTAIMDAGGGFQNFPDDYGVTNGLCKDSDLTIRMPYYLFAQKAGTELNDYTKWM
HHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCHHEEHHHCCCCHHHHHHHH
QTVEIGEGCDDDHHSDKVEYHVQGAGENLVMSAGDFENFDKPRPELSPAMEGQLKEVLSL
HHHHCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
LVKNRWPFRIHATYNESITRFLNVIEDINKETPLNGLLWFFDHAETVSTENLKRIKALNG
HHCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCC
GIAIQHRMAYQGESFIKRYGKTAAANTVPLKKMFELGIKVGMGTDGTRVASYNPWVGLYW
CEEEEEEHHHCCHHHHHHHCCCCCCCCCCHHHHHHCCEEEECCCCCCEEEECCCCEEEEE
LTTGKTLGGLKYMNDENIVDRTTALKLFTYGSAQLINIEKDRGMLTADKLADFSILSDDY
EECCCCCCCEEECCCCCCHHHHEEEEEEECCCEEEEEEECCCCCEEHHHHCCEEEECCCC
FTTSEENILNIESKLTVVNGKAVYADNDFRTFVNEKPKAIPDWSPVNYFGGYQKN
CCCCCCCEEEEEEEEEEEECEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA