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The map label for this gene is degA [H]

Identifier: 146301993

GI number: 146301993

Start: 5141837

End: 5142865

Strand: Direct

Name: degA [H]

Synonym: Fjoh_4257

Alternate gene names: 146301993

Gene position: 5141837-5142865 (Clockwise)

Preceding gene: 146301979

Following gene: 146302002

Centisome position: 84.34

GC content: 35.96

Gene sequence:

>1029_bases
ATGAGCGAAAAAGTAACCATTTATGATATTGCCGAAAAACTAAATATCACTGCAGCTACTGTTTCCAGAGCGTTGAATAA
CAACCCGAAAATAAAAGAAAGCACTCGTGAGTTGGTTATTAAAACTGCTGCCTCAATGAACTACAAGCAAAACAAACTTG
CTTTAGCATTAAAAAGCGGCCGAAGTAATAATATTGGAGTGATTGTACCACGTATTGACAGCAACTTTTTTGCTTCTGTT
ATTCGTGGTATTGAAGAAGAATTACAGCCTCACGGTTATCAGGTAATTATCTGTCAGACTCATGAAAGCAAACAGAGAGA
AAATGAAAATCTATATACTTTAATTGATGCTCAGGTTGACGGCATTCTGATGTCGGTTACCGATGTAACCAGCGAAAATG
ATGATGCTTTTAACAATGTGTTACAAAAGAATGTACCTCTGATCTTTTTTGACAGGAGCAAACATATTGACGGCGTGAGC
TCTGTAACTATTAACGATTTTAGAGGCGGTTATTTAACCACTAAACATTTAATTAACGAAGGATGCCGTTATATTGCCCA
TTTTTCTGGAGATCAGTCCCTGGAAATCTTTAAAAACAGGTTTTTAGGCTACAAACAAGCTCTTTTAGATCACGGAATTG
AATTTAAAGAAGAATATGTTATACCAGTAAAAAGTATGGTTGACGCTGGAAAAGAAGCAGTCGATAAACTGCTGCAATTA
GAAACACCGCCTGATGCTATATTCTCGTCGAGTGACTTTGCTGCTTTAGGAGCAATTCAGGAATTAAAAGAGAGAAATAT
TAGCATTCCGAATGAGTTTTGTGTGGCTGGTTTTAGTAACGAACCTTTTACCAAATTCATGGAATTATCAATTACATCTG
TAGATCAGTCACCGCTTGAAATGGGAAAAATGGCTGCACGTGTTTTCTTAGAACAAGTCGATAAAACTGATACTATTAAA
ATAGAAAAAAAGGTGGTTTTAGCACCTGAATTACACATTCGTAAATCATCTTCGAGAACAAACTTTTAA

Upstream 100 bases:

>100_bases
CGTTGTAGTAATTCGTTATGGTAATCGATAACATTAAACAACAGATTATATAAAAAAAATGTATTTTTGTCGTAGAATTT
AACTTGTAAACCCCTTTTTA

Downstream 100 bases:

>100_bases
AATATATTTTACCTTAATAAATTATAAGCTCATTTAACGATGAGCTTATTTTTTTGCCATAAACAATACTAAATCACTCC
TATGGAGCTTATATCATGAT

Product: periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSEKVTIYDIAEKLNITAATVSRALNNNPKIKESTRELVIKTAASMNYKQNKLALALKSGRSNNIGVIVPRIDSNFFASV
IRGIEEELQPHGYQVIICQTHESKQRENENLYTLIDAQVDGILMSVTDVTSENDDAFNNVLQKNVPLIFFDRSKHIDGVS
SVTINDFRGGYLTTKHLINEGCRYIAHFSGDQSLEIFKNRFLGYKQALLDHGIEFKEEYVIPVKSMVDAGKEAVDKLLQL
ETPPDAIFSSSDFAALGAIQELKERNISIPNEFCVAGFSNEPFTKFMELSITSVDQSPLEMGKMAARVFLEQVDKTDTIK
IEKKVVLAPELHIRKSSSRTNF

Sequences:

>Translated_342_residues
MSEKVTIYDIAEKLNITAATVSRALNNNPKIKESTRELVIKTAASMNYKQNKLALALKSGRSNNIGVIVPRIDSNFFASV
IRGIEEELQPHGYQVIICQTHESKQRENENLYTLIDAQVDGILMSVTDVTSENDDAFNNVLQKNVPLIFFDRSKHIDGVS
SVTINDFRGGYLTTKHLINEGCRYIAHFSGDQSLEIFKNRFLGYKQALLDHGIEFKEEYVIPVKSMVDAGKEAVDKLLQL
ETPPDAIFSSSDFAALGAIQELKERNISIPNEFCVAGFSNEPFTKFMELSITSVDQSPLEMGKMAARVFLEQVDKTDTIK
IEKKVVLAPELHIRKSSSRTNF
>Mature_341_residues
SEKVTIYDIAEKLNITAATVSRALNNNPKIKESTRELVIKTAASMNYKQNKLALALKSGRSNNIGVIVPRIDSNFFASVI
RGIEEELQPHGYQVIICQTHESKQRENENLYTLIDAQVDGILMSVTDVTSENDDAFNNVLQKNVPLIFFDRSKHIDGVSS
VTINDFRGGYLTTKHLINEGCRYIAHFSGDQSLEIFKNRFLGYKQALLDHGIEFKEEYVIPVKSMVDAGKEAVDKLLQLE
TPPDAIFSSSDFAALGAIQELKERNISIPNEFCVAGFSNEPFTKFMELSITSVDQSPLEMGKMAARVFLEQVDKTDTIKI
EKKVVLAPELHIRKSSSRTNF

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=311, Percent_Identity=30.5466237942122, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1790194, Length=335, Percent_Identity=26.865671641791, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788474, Length=315, Percent_Identity=26.6666666666667, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1787948, Length=328, Percent_Identity=22.5609756097561, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787906, Length=326, Percent_Identity=24.5398773006135, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=26.6666666666667, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI48994940, Length=318, Percent_Identity=24.8427672955975, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789202, Length=290, Percent_Identity=25.5172413793103, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789068, Length=299, Percent_Identity=25.0836120401338, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790715, Length=324, Percent_Identity=22.8395061728395, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790689, Length=319, Percent_Identity=26.3322884012539, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786268, Length=311, Percent_Identity=21.2218649517685, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=23.2142857142857, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38345; Mature: 38214

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKVTIYDIAEKLNITAATVSRALNNNPKIKESTRELVIKTAASMNYKQNKLALALKSG
CCCCEEEEEHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECC
RSNNIGVIVPRIDSNFFASVIRGIEEELQPHGYQVIICQTHESKQRENENLYTLIDAQVD
CCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHCCCCEEEEEECCCC
GILMSVTDVTSENDDAFNNVLQKNVPLIFFDRSKHIDGVSSVTINDFRGGYLTTKHLINE
CEEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHH
GCRYIAHFSGDQSLEIFKNRFLGYKQALLDHGIEFKEEYVIPVKSMVDAGKEAVDKLLQL
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHEECCHHHHHHCCHHHHHHHHHC
ETPPDAIFSSSDFAALGAIQELKERNISIPNEFCVAGFSNEPFTKFMELSITSVDQSPLE
CCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCHHEEECCCCCCHHHHHHEEEECCCCCHHH
MGKMAARVFLEQVDKTDTIKIEKKVVLAPELHIRKSSSRTNF
HHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEECCCCCCCC
>Mature Secondary Structure 
SEKVTIYDIAEKLNITAATVSRALNNNPKIKESTRELVIKTAASMNYKQNKLALALKSG
CCCEEEEEHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECC
RSNNIGVIVPRIDSNFFASVIRGIEEELQPHGYQVIICQTHESKQRENENLYTLIDAQVD
CCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHCCCCEEEEEECCCC
GILMSVTDVTSENDDAFNNVLQKNVPLIFFDRSKHIDGVSSVTINDFRGGYLTTKHLINE
CEEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHH
GCRYIAHFSGDQSLEIFKNRFLGYKQALLDHGIEFKEEYVIPVKSMVDAGKEAVDKLLQL
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHEECCHHHHHHCCHHHHHHHHHC
ETPPDAIFSSSDFAALGAIQELKERNISIPNEFCVAGFSNEPFTKFMELSITSVDQSPLE
CCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCHHEEECCCCCCHHHHHHEEEECCCCCHHH
MGKMAARVFLEQVDKTDTIKIEKKVVLAPELHIRKSSSRTNF
HHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]