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The map label for this gene is crp [H]

Identifier: 146301789

GI number: 146301789

Start: 4838730

End: 4839365

Strand: Direct

Name: crp [H]

Synonym: Fjoh_4053

Alternate gene names: 146301789

Gene position: 4838730-4839365 (Clockwise)

Preceding gene: 146301788

Following gene: 146301791

Centisome position: 79.36

GC content: 32.7

Gene sequence:

>636_bases
ATGATCCTTAATCATTTTAATTGCAGTAATTGTCGACAAATGCATATTGATACTGATTTACTTTATACCTGGGGAGCCGT
TGCAAAAAAAGTCCCAAAACATGCCGTCATCTTTTTCGAGAATGACCCGCCCCTTTCCTATTATCAAATTTTAGAGGGAA
CCGTTAAAATGAGTTATACCAATGAAGACGGCAAAGATCTTACAATTGGTATTTTTAATGAAGGAAATAGTTTTGGAGAA
CCGCCCTTATTTATTGAGCAGCCCTACCCTGCTTCTGCAGAAGCTCAAACTGATTGTGTAATTTTAAAACTATCAAAAAC
CAATTTCTTTTCGCTGCTTGAAGAAAATCCTGAAATACAATCAAGGGTTTTAAAACTCTTTGCGTGGAAAATTTACAATA
AAATTGTTTTCTCCAAAAATATAATAAATCACAAACCAGAATACCGCATTCAATATTTTCTGGATAATTTAAAAAGACAG
CATAACAGCAAACCTTCTCAGAAAATGAAAATTCCTTATACAAGACAGGAGATTGCAGATTTCACCGGTCTTCGTGTAGA
AACAGTCATTAGAACAATTTCATTAATGAATAAAAACAAAAAAATCGAAATCATCGATCATAAATTGTTTTACTAA

Upstream 100 bases:

>100_bases
ATTTTAAAGTTCTCAAAACCTAACATTTATCATATTTTAATTTTAAGTCAATGCTTTCCTTTGCTGTAACACTACAAAAT
ATATTGGAGTTTAAAAAAGA

Downstream 100 bases:

>100_bases
CGTCACTTTACGATTAAGTTTAAAAATAATAAAGTATGATTCAAATCATATTTCTGAAAAAATGGTTTGTCTTTCTTTGT
AAAGGTTAAAGAAAATTATT

Product: CRP/FNR family transcriptional regulator

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MILNHFNCSNCRQMHIDTDLLYTWGAVAKKVPKHAVIFFENDPPLSYYQILEGTVKMSYTNEDGKDLTIGIFNEGNSFGE
PPLFIEQPYPASAEAQTDCVILKLSKTNFFSLLEENPEIQSRVLKLFAWKIYNKIVFSKNIINHKPEYRIQYFLDNLKRQ
HNSKPSQKMKIPYTRQEIADFTGLRVETVIRTISLMNKNKKIEIIDHKLFY

Sequences:

>Translated_211_residues
MILNHFNCSNCRQMHIDTDLLYTWGAVAKKVPKHAVIFFENDPPLSYYQILEGTVKMSYTNEDGKDLTIGIFNEGNSFGE
PPLFIEQPYPASAEAQTDCVILKLSKTNFFSLLEENPEIQSRVLKLFAWKIYNKIVFSKNIINHKPEYRIQYFLDNLKRQ
HNSKPSQKMKIPYTRQEIADFTGLRVETVIRTISLMNKNKKIEIIDHKLFY
>Mature_211_residues
MILNHFNCSNCRQMHIDTDLLYTWGAVAKKVPKHAVIFFENDPPLSYYQILEGTVKMSYTNEDGKDLTIGIFNEGNSFGE
PPLFIEQPYPASAEAQTDCVILKLSKTNFFSLLEENPEIQSRVLKLFAWKIYNKIVFSKNIINHKPEYRIQYFLDNLKRQ
HNSKPSQKMKIPYTRQEIADFTGLRVETVIRTISLMNKNKKIEIIDHKLFY

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=169, Percent_Identity=28.4023668639053, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24702; Mature: 24702

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILNHFNCSNCRQMHIDTDLLYTWGAVAKKVPKHAVIFFENDPPLSYYQILEGTVKMSYT
CEECCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCEEEEEEE
NEDGKDLTIGIFNEGNSFGEPPLFIEQPYPASAEAQTDCVILKLSKTNFFSLLEENPEIQ
CCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHCCHHHH
SRVLKLFAWKIYNKIVFSKNIINHKPEYRIQYFLDNLKRQHNSKPSQKMKIPYTRQEIAD
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHH
FTGLRVETVIRTISLMNKNKKIEIIDHKLFY
HCCCHHHHHHHHHHHHCCCCEEEEECCEECC
>Mature Secondary Structure
MILNHFNCSNCRQMHIDTDLLYTWGAVAKKVPKHAVIFFENDPPLSYYQILEGTVKMSYT
CEECCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCEEEEEEE
NEDGKDLTIGIFNEGNSFGEPPLFIEQPYPASAEAQTDCVILKLSKTNFFSLLEENPEIQ
CCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHCCHHHH
SRVLKLFAWKIYNKIVFSKNIINHKPEYRIQYFLDNLKRQHNSKPSQKMKIPYTRQEIAD
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHH
FTGLRVETVIRTISLMNKNKKIEIIDHKLFY
HCCCHHHHHHHHHHHHCCCCEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]