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The map label for this gene is tam [H]

Identifier: 146301677

GI number: 146301677

Start: 4693024

End: 4693791

Strand: Reverse

Name: tam [H]

Synonym: Fjoh_3939

Alternate gene names: 146301677

Gene position: 4693791-4693024 (Counterclockwise)

Preceding gene: 146301679

Following gene: 146301676

Centisome position: 76.99

GC content: 33.46

Gene sequence:

>768_bases
ATGCCTTGGAATCCAGAAATATACAATACATTTAAAGACATTCGTTATAAACCATTTTATGATTTGGCTGATTTGATAAA
GCCAGTCAAAAATAAGAAAGCCATTGATTTAGGATGCGGTACAGGCGAACAAACCGCTATTTTAGCAGATAAATTTAAAG
AAGTCCATTTTCTTGGTGTTGATTCTTCGGCAGAAATGCTCGAAAAATCAAAAGCATTAGAAACAGAAAACCTGCATTTT
CGCAGAGCATCAACCGAAGAAATGATTGAAAATGCCGAAAAATGGGATTTAATTTTTAGCAATGCCGCTTTGCAATGGTC
GAATAATCATGAAATTTTGTTTCCTAAATTGATACACCTTCTTAATTCAAAAGGGCAGTTTGCAGTTCAAATGCCGATGC
AACCAGAAAATAAACTCAACAAAATTTTAATCGAATTGGTTAACGAAGAACCTTTTAAAACGTATCTAAAAGGCTTTAAA
AGAGATTCACCCGTTTTGAGTATTGATGAATATGCGCAGATACTATTTGACAGCGGACTCGAAGATATTCAAATCATGCA
GAAAGTGTATCCCATAATTGCAAACGATCACGATACGTTGTATAATTTCATTTCAGGTTCAGCTTTAATTCCATACATGG
AAAAACTTGAAGGCGGACAGAAAGAACTTTTTATAAAAACATACAAAGAAAGAATAGCAGAAAGTTTTCCTAAACTTCCG
GCAATTTATTCTTTTAAAAGACTTTTGCTTTACGGAACAAAAGCATAA

Upstream 100 bases:

>100_bases
AATTCTGAATGCTAAAACAAGCAGGAAATTTGTACTTTAGTAAAACACTTGCATTTAGTTAACGCTGTAAGTTGTTTTTT
TAATATAAAAATTGATATAA

Downstream 100 bases:

>100_bases
ATACTTATTTTCTAAACACATTACAATATGAAAGCATTAGTAATTGGGGCGACAGGCTCAACAGGAAAGTTTTTAACAGA
AGAGCTTCTTAATGATAACG

Product: trans-aconitate 2-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MPWNPEIYNTFKDIRYKPFYDLADLIKPVKNKKAIDLGCGTGEQTAILADKFKEVHFLGVDSSAEMLEKSKALETENLHF
RRASTEEMIENAEKWDLIFSNAALQWSNNHEILFPKLIHLLNSKGQFAVQMPMQPENKLNKILIELVNEEPFKTYLKGFK
RDSPVLSIDEYAQILFDSGLEDIQIMQKVYPIIANDHDTLYNFISGSALIPYMEKLEGGQKELFIKTYKERIAESFPKLP
AIYSFKRLLLYGTKA

Sequences:

>Translated_255_residues
MPWNPEIYNTFKDIRYKPFYDLADLIKPVKNKKAIDLGCGTGEQTAILADKFKEVHFLGVDSSAEMLEKSKALETENLHF
RRASTEEMIENAEKWDLIFSNAALQWSNNHEILFPKLIHLLNSKGQFAVQMPMQPENKLNKILIELVNEEPFKTYLKGFK
RDSPVLSIDEYAQILFDSGLEDIQIMQKVYPIIANDHDTLYNFISGSALIPYMEKLEGGQKELFIKTYKERIAESFPKLP
AIYSFKRLLLYGTKA
>Mature_254_residues
PWNPEIYNTFKDIRYKPFYDLADLIKPVKNKKAIDLGCGTGEQTAILADKFKEVHFLGVDSSAEMLEKSKALETENLHFR
RASTEEMIENAEKWDLIFSNAALQWSNNHEILFPKLIHLLNSKGQFAVQMPMQPENKLNKILIELVNEEPFKTYLKGFKR
DSPVLSIDEYAQILFDSGLEDIQIMQKVYPIIANDHDTLYNFISGSALIPYMEKLEGGQKELFIKTYKERIAESFPKLPA
IYSFKRLLLYGTKA

Specific function: Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate [H]

COG id: COG4106

COG function: function code R; Trans-aconitate methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Tam family [H]

Homologues:

Organism=Escherichia coli, GI1787798, Length=236, Percent_Identity=29.2372881355932, Blast_Score=118, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR023149 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.144 [H]

Molecular weight: Translated: 29465; Mature: 29334

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWNPEIYNTFKDIRYKPFYDLADLIKPVKNKKAIDLGCGTGEQTAILADKFKEVHFLGV
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHEEEEEC
DSSAEMLEKSKALETENLHFRRASTEEMIENAEKWDLIFSNAALQWSNNHEILFPKLIHL
CCHHHHHHHHHCCCCCCCEEECCCHHHHHCCHHHHHEEEECCEEEECCCCCCHHHHHHHH
LNSKGQFAVQMPMQPENKLNKILIELVNEEPFKTYLKGFKRDSPVLSIDEYAQILFDSGL
HCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
EDIQIMQKVYPIIANDHDTLYNFISGSALIPYMEKLEGGQKELFIKTYKERIAESFPKLP
HHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCH
AIYSFKRLLLYGTKA
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
PWNPEIYNTFKDIRYKPFYDLADLIKPVKNKKAIDLGCGTGEQTAILADKFKEVHFLGV
CCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHEEEEEC
DSSAEMLEKSKALETENLHFRRASTEEMIENAEKWDLIFSNAALQWSNNHEILFPKLIHL
CCHHHHHHHHHCCCCCCCEEECCCHHHHHCCHHHHHEEEECCEEEECCCCCCHHHHHHHH
LNSKGQFAVQMPMQPENKLNKILIELVNEEPFKTYLKGFKRDSPVLSIDEYAQILFDSGL
HCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
EDIQIMQKVYPIIANDHDTLYNFISGSALIPYMEKLEGGQKELFIKTYKERIAESFPKLP
HHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCH
AIYSFKRLLLYGTKA
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]