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The map label for this gene is tpiA

Identifier: 146301246

GI number: 146301246

Start: 4178433

End: 4179185

Strand: Direct

Name: tpiA

Synonym: Fjoh_3504

Alternate gene names: 146301246

Gene position: 4178433-4179185 (Clockwise)

Preceding gene: 146301245

Following gene: 146301249

Centisome position: 68.53

GC content: 36.79

Gene sequence:

>753_bases
ATGAGAAAATCGATTGTTGCAGGAAACTGGAAAATGCATAAAAATGCAGCGCAGACTGAAGAATTATTAAACGAATTAAT
TGCAAAAATTCCAGCAAAAACAAATGCACAAGTTATTGTAGCGCCAACTTTTGTAAACTTACAAGCTGCTGCTGCTAAAT
TAAAAAATACAACTATTGGAGTTTCTGCTCAAAACGTTCACCAAGCTGAAGGCGGTGCTTTTACAGGAGAAATTTCTGCA
GATATGTTAACAAGCATTGGTGTTAATACTGTAATCTTAGGTCACTCTGAGCGTAGAGCTATTTTCCACGAAACTGATGC
TTTAATCGCAAACAAAGTTGATACTGCTTTAAAACACGATATGACAGTAATTTTCTGTTTTGGAGAAGAATTAAAAGACC
GTCAGTCTGGAAATCATTTCAACATTGTTGAAAATCAATTACGTGACGGAGTTTTCCATATCGCAAAAGAATCTTGGTCT
AAAATTGTTTTAGCTTACGAACCAGTTTGGGCTATCGGAACTGGAGAAACTGCTTCACCAGAACAAGCTCAGGAAATGCA
CGAATTTATCAGAGAAACGATCCGCAAAGCTTTTGGAGCTGAAATCGCAGATGAAGTTTCTATTTTATACGGTGGTTCTG
TAAAACCAGAAAACGCTAAAGAAATCTTCTCTAAACCAGACGTAGATGGTGGTTTAATTGGTGGTGCAGCTTTAAAAGCT
GACGATTTCTTAGCAATTGTAACTGCTATCTAA

Upstream 100 bases:

>100_bases
GCTTAAAATAAATTTAAAATAACAGCAATTTTAAAATTGCTGTTATTTTAATCTACTTCATTCAAACAAAAAAATTAAAA
GAATAACAAAAACTACCAAA

Downstream 100 bases:

>100_bases
TTTTAGATTTTAGATTTTGAAATCCTCTTATAAAAAAAGCGTTCGCATAGCGAACGCTTTTTTTATATTCTCCATTGACT
TTTGACTTTTGACTTTCGAC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MRKSIVAGNWKMHKNAAQTEELLNELIAKIPAKTNAQVIVAPTFVNLQAAAAKLKNTTIGVSAQNVHQAEGGAFTGEISA
DMLTSIGVNTVILGHSERRAIFHETDALIANKVDTALKHDMTVIFCFGEELKDRQSGNHFNIVENQLRDGVFHIAKESWS
KIVLAYEPVWAIGTGETASPEQAQEMHEFIRETIRKAFGAEIADEVSILYGGSVKPENAKEIFSKPDVDGGLIGGAALKA
DDFLAIVTAI

Sequences:

>Translated_250_residues
MRKSIVAGNWKMHKNAAQTEELLNELIAKIPAKTNAQVIVAPTFVNLQAAAAKLKNTTIGVSAQNVHQAEGGAFTGEISA
DMLTSIGVNTVILGHSERRAIFHETDALIANKVDTALKHDMTVIFCFGEELKDRQSGNHFNIVENQLRDGVFHIAKESWS
KIVLAYEPVWAIGTGETASPEQAQEMHEFIRETIRKAFGAEIADEVSILYGGSVKPENAKEIFSKPDVDGGLIGGAALKA
DDFLAIVTAI
>Mature_250_residues
MRKSIVAGNWKMHKNAAQTEELLNELIAKIPAKTNAQVIVAPTFVNLQAAAAKLKNTTIGVSAQNVHQAEGGAFTGEISA
DMLTSIGVNTVILGHSERRAIFHETDALIANKVDTALKHDMTVIFCFGEELKDRQSGNHFNIVENQLRDGVFHIAKESWS
KIVLAYEPVWAIGTGETASPEQAQEMHEFIRETIRKAFGAEIADEVSILYGGSVKPENAKEIFSKPDVDGGLIGGAALKA
DDFLAIVTAI

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=249, Percent_Identity=46.1847389558233, Blast_Score=204, Evalue=5e-53,
Organism=Homo sapiens, GI226529917, Length=249, Percent_Identity=46.1847389558233, Blast_Score=204, Evalue=6e-53,
Organism=Escherichia coli, GI1790353, Length=250, Percent_Identity=42, Blast_Score=190, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI17536593, Length=252, Percent_Identity=46.8253968253968, Blast_Score=210, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6320255, Length=249, Percent_Identity=41.7670682730924, Blast_Score=189, Evalue=3e-49,
Organism=Drosophila melanogaster, GI28572008, Length=248, Percent_Identity=44.3548387096774, Blast_Score=203, Evalue=7e-53,
Organism=Drosophila melanogaster, GI28572006, Length=248, Percent_Identity=44.3548387096774, Blast_Score=203, Evalue=7e-53,
Organism=Drosophila melanogaster, GI28572004, Length=248, Percent_Identity=43.9516129032258, Blast_Score=202, Evalue=1e-52,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_FLAJ1 (A5FE44)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001195837.1
- ProteinModelPortal:   A5FE44
- SMR:   A5FE44
- STRING:   A5FE44
- GeneID:   5091182
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_3504
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   FJOH376686:FJOH_3504-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27036; Mature: 27036

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSIVAGNWKMHKNAAQTEELLNELIAKIPAKTNAQVIVAPTFVNLQAAAAKLKNTTIG
CCCCEEECCCEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHHHHHHHCCCEEE
VSAQNVHQAEGGAFTGEISADMLTSIGVNTVILGHSERRAIFHETDALIANKVDTALKHD
CCHHHHHHCCCCEEECCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
MTVIFCFGEELKDRQSGNHFNIVENQLRDGVFHIAKESWSKIVLAYEPVWAIGTGETASP
CEEEEEECHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCEEEEEECCEEEECCCCCCCH
EQAQEMHEFIRETIRKAFGAEIADEVSILYGGSVKPENAKEIFSKPDVDGGLIGGAALKA
HHHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCCCCCHHHHHCCCCCCCCEECCCEECC
DDFLAIVTAI
CCCEEEEECC
>Mature Secondary Structure
MRKSIVAGNWKMHKNAAQTEELLNELIAKIPAKTNAQVIVAPTFVNLQAAAAKLKNTTIG
CCCCEEECCCEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHHHHHHHCCCEEE
VSAQNVHQAEGGAFTGEISADMLTSIGVNTVILGHSERRAIFHETDALIANKVDTALKHD
CCHHHHHHCCCCEEECCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
MTVIFCFGEELKDRQSGNHFNIVENQLRDGVFHIAKESWSKIVLAYEPVWAIGTGETASP
CEEEEEECHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCEEEEEECCEEEECCCCCCCH
EQAQEMHEFIRETIRKAFGAEIADEVSILYGGSVKPENAKEIFSKPDVDGGLIGGAALKA
HHHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCCCCCHHHHHCCCCCCCCEECCCEECC
DDFLAIVTAI
CCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA