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The map label for this gene is yfkN [H]

Identifier: 146301215

GI number: 146301215

Start: 4138989

End: 4139900

Strand: Direct

Name: yfkN [H]

Synonym: Fjoh_3473

Alternate gene names: 146301215

Gene position: 4138989-4139900 (Clockwise)

Preceding gene: 146301214

Following gene: 146301225

Centisome position: 67.89

GC content: 33.55

Gene sequence:

>912_bases
ATGAAAAGAAGAGAATTTATCGAAAAAACAGCTGCAAGTACTGCCTTATTAAGCTTAGGATTGTCTTTGAGCAGTTTTGA
AACTAACGATATTAAACACTTAACTGTTCTTCATACTAATGATGTTCACAGTCATATTGATCCTTTTCCGGCTGATGATC
CTCGTAATCCAAATAAAGGCGGTGTTTCTCGTCGTGCAGCACTTATTGAAACTATTCGTCAGGAAAATCCAAATGTGCTT
TTATTAGATGCTGGAGATATTTTTCAGGGAACTCCGTATTTTAATTATTACGGCGGCGAACTTGAGTTCAAACTGATGAG
CATGATGAAATATGATGCTTCTACAATTGGAAATCATGATTTTGATAATGGTCTTGATGGTTTGTATGCTCAAATGCCTC
ATGCAACTTTTGAATTTATAAATTCTAATTACGATTTTAAAAATACGGTTATGAACGGCCTTGTTAAACCGTATAAAATC
TTTAATAAAAACGGAATTAAGGTTGGTGTTTTTGGTGTAGGAATTGAACTTCAGGGTTTAGTTGACAAACAATTATATAA
GGAAACTGTTTATAATGATCCTGTTGAAATTGCTCAGGATATGACGAAATTATTAAAGAAAGAAGAAAAATGTGATTTGG
TTATCTGCCTTTCGCATCTTGGGTATAAATATAAAGATGACGAAAATAAGATTTCAGATTTAAAATTTGCTGAACTTACA
CAGGATATTGATTTGATTATTGGCGGTCACACGCATACTTTTCTTGACAAACCTACAATTGTAAAAAACAAAGCAGGACA
AGAGGTACTGGTTAATCAGGTTGGTTGTTACGGAATTAATTTAGGCCGTATCGATTTTTATTTTGACAAAGATAAAGCGC
ACAGCAACCAGGCGAGATCAATTGTTGTATAA

Upstream 100 bases:

>100_bases
AAGTTAAGACAATCCCGGTATCAAAAGATATTAGAATTACAGAAGAATAAACAAATCAAATTAAGAAAAAACAAAAAGCG
TTACGCAAAAAATATACAAG

Downstream 100 bases:

>100_bases
GGATCTTCGCTTCATTTTAGTTTTTTCGAGAAAATATTCTACCATAAAATAGTAGGCGAGAATTTGTGTTACATCGCGAA
TTAAAACCAGCACAACTGAA

Product: metallophosphoesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKRREFIEKTAASTALLSLGLSLSSFETNDIKHLTVLHTNDVHSHIDPFPADDPRNPNKGGVSRRAALIETIRQENPNVL
LLDAGDIFQGTPYFNYYGGELEFKLMSMMKYDASTIGNHDFDNGLDGLYAQMPHATFEFINSNYDFKNTVMNGLVKPYKI
FNKNGIKVGVFGVGIELQGLVDKQLYKETVYNDPVEIAQDMTKLLKKEEKCDLVICLSHLGYKYKDDENKISDLKFAELT
QDIDLIIGGHTHTFLDKPTIVKNKAGQEVLVNQVGCYGINLGRIDFYFDKDKAHSNQARSIVV

Sequences:

>Translated_303_residues
MKRREFIEKTAASTALLSLGLSLSSFETNDIKHLTVLHTNDVHSHIDPFPADDPRNPNKGGVSRRAALIETIRQENPNVL
LLDAGDIFQGTPYFNYYGGELEFKLMSMMKYDASTIGNHDFDNGLDGLYAQMPHATFEFINSNYDFKNTVMNGLVKPYKI
FNKNGIKVGVFGVGIELQGLVDKQLYKETVYNDPVEIAQDMTKLLKKEEKCDLVICLSHLGYKYKDDENKISDLKFAELT
QDIDLIIGGHTHTFLDKPTIVKNKAGQEVLVNQVGCYGINLGRIDFYFDKDKAHSNQARSIVV
>Mature_303_residues
MKRREFIEKTAASTALLSLGLSLSSFETNDIKHLTVLHTNDVHSHIDPFPADDPRNPNKGGVSRRAALIETIRQENPNVL
LLDAGDIFQGTPYFNYYGGELEFKLMSMMKYDASTIGNHDFDNGLDGLYAQMPHATFEFINSNYDFKNTVMNGLVKPYKI
FNKNGIKVGVFGVGIELQGLVDKQLYKETVYNDPVEIAQDMTKLLKKEEKCDLVICLSHLGYKYKDDENKISDLKFAELT
QDIDLIIGGHTHTFLDKPTIVKNKAGQEVLVNQVGCYGINLGRIDFYFDKDKAHSNQARSIVV

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=301, Percent_Identity=31.8936877076412, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1786687, Length=287, Percent_Identity=27.5261324041812, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790658, Length=297, Percent_Identity=25.9259259259259, Blast_Score=79, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922446, Length=285, Percent_Identity=30.1754385964912, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24654424, Length=285, Percent_Identity=30.1754385964912, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28573524, Length=281, Percent_Identity=28.8256227758007, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI221329836, Length=276, Percent_Identity=26.8115942028986, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24641187, Length=276, Percent_Identity=26.8115942028986, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922444, Length=293, Percent_Identity=27.6450511945392, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19921980, Length=233, Percent_Identity=30.4721030042918, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24652512, Length=233, Percent_Identity=30.4721030042918, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI161076508, Length=233, Percent_Identity=30.4721030042918, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 34137; Mature: 34137

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRREFIEKTAASTALLSLGLSLSSFETNDIKHLTVLHTNDVHSHIDPFPADDPRNPNKG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHCCCCCCCCCCCCCCCC
GVSRRAALIETIRQENPNVLLLDAGDIFQGTPYFNYYGGELEFKLMSMMKYDASTIGNHD
CHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCEEHHHHHHHHHHHHHHCCCCC
FDNGLDGLYAQMPHATFEFINSNYDFKNTVMNGLVKPYKIFNKNGIKVGVFGVGIELQGL
CCCCCCHHHHHCCCHHHHHHCCCCCHHHHHHHHCCCCCEEECCCCCEEEEEEECEEEEHH
VDKQLYKETVYNDPVEIAQDMTKLLKKEEKCDLVICLSHLGYKYKDDENKISDLKFAELT
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEHHCCCEECCCCCHHHCCHHHHHH
QDIDLIIGGHTHTFLDKPTIVKNKAGQEVLVNQVGCYGINLGRIDFYFDKDKAHSNQARS
CCEEEEECCCEEEECCCCCEECCCCCHHHHHHCCCEEEEEECCEEEEECCCCCCCCCCCE
IVV
ECC
>Mature Secondary Structure
MKRREFIEKTAASTALLSLGLSLSSFETNDIKHLTVLHTNDVHSHIDPFPADDPRNPNKG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHCCCCCCCCCCCCCCCC
GVSRRAALIETIRQENPNVLLLDAGDIFQGTPYFNYYGGELEFKLMSMMKYDASTIGNHD
CHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCEEHHHHHHHHHHHHHHCCCCC
FDNGLDGLYAQMPHATFEFINSNYDFKNTVMNGLVKPYKIFNKNGIKVGVFGVGIELQGL
CCCCCCHHHHHCCCHHHHHHCCCCCHHHHHHHHCCCCCEEECCCCCEEEEEEECEEEEHH
VDKQLYKETVYNDPVEIAQDMTKLLKKEEKCDLVICLSHLGYKYKDDENKISDLKFAELT
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEHHCCCEECCCCCHHHCCHHHHHH
QDIDLIIGGHTHTFLDKPTIVKNKAGQEVLVNQVGCYGINLGRIDFYFDKDKAHSNQARS
CCEEEEECCCEEEECCCCCEECCCCCHHHHHHCCCEEEEEECCEEEEECCCCCCCCCCCE
IVV
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]