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The map label for this gene is yfkN [H]

Identifier: 146301214

GI number: 146301214

Start: 4138159

End: 4138938

Strand: Direct

Name: yfkN [H]

Synonym: Fjoh_3472

Alternate gene names: 146301214

Gene position: 4138159-4138938 (Clockwise)

Preceding gene: 146301196

Following gene: 146301215

Centisome position: 67.87

GC content: 30.13

Gene sequence:

>780_bases
ATGGTAAAACTAAAAAAGTATAACCGATTTTTAAAACTTTTTGTTATATTCTTAACACTATTTTCAATAATCTCTTGCAG
CAACAAGAATTATAATTTAACAAAAATAGAAGGAAAACAGATTCCGGTTACTGAAAAAGGTACCGAAACTCCTGAAATTG
AAAAATTTATTAAGCCTTATCGTGATCATATTAATAAAGATCTGGACAGTGTTCTTGCCTACTGCCCTGAAAATTTAGAT
AAAAACTCGGGGAAATGGCAGACTACTATTGGCAACATGCTTGCTGATGCTTGTGTACAGCGCGGTGATATTATTTTTAA
ATCCAGAGAAAATAAAACCATTGACTTCTGCCTTTTAAATTTTGGAGGTATTAGAGCTGGTCTTCCAAAAGGAAATGTTA
CCAGCAGAAGTGCTTTTGAAATTATGCCGTTTGAAAACAATTTAGTTGTACTGGCATTAAAAGGAGAGCAAGTTCTTGAA
ATAACTTCTTATTTTATTAAAACACAAAAAGCACAACCATTATCTGGTATGACTTTTACTATTGCAAAAGATAAAACTGT
TAAAAATATTTTGATACAAGGCAAACCTTTTGATATAAACAAAACATATTATGTTGCTACGAATGATTATTTAGCAAATG
GTGGTGACAATATGAGTTTTTTCGCAAAAAACACACAGAAATTTGATTTGAATTATAAACTTCGAAATGTGCTGATTGAT
TATTTTAAACAAGTTAAGACAATCCCGGTATCAAAAGATATTAGAATTACAGAAGAATAA

Upstream 100 bases:

>100_bases
GTTATTTTTTAATGATTTTTTTACAAAAAATTCCTTTATATAATTTAAAAACATAGTACTTTTACAAATTACAAAATTAA
TTATTTAAGCCGCATTTAAG

Downstream 100 bases:

>100_bases
ACAAATCAAATTAAGAAAAAACAAAAAGCGTTACGCAAAAAATATACAAGATGAAAAGAAGAGAATTTATCGAAAAAACA
GCTGCAAGTACTGCCTTATT

Product: 5'-nucleotidase domain-containing protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MVKLKKYNRFLKLFVIFLTLFSIISCSNKNYNLTKIEGKQIPVTEKGTETPEIEKFIKPYRDHINKDLDSVLAYCPENLD
KNSGKWQTTIGNMLADACVQRGDIIFKSRENKTIDFCLLNFGGIRAGLPKGNVTSRSAFEIMPFENNLVVLALKGEQVLE
ITSYFIKTQKAQPLSGMTFTIAKDKTVKNILIQGKPFDINKTYYVATNDYLANGGDNMSFFAKNTQKFDLNYKLRNVLID
YFKQVKTIPVSKDIRITEE

Sequences:

>Translated_259_residues
MVKLKKYNRFLKLFVIFLTLFSIISCSNKNYNLTKIEGKQIPVTEKGTETPEIEKFIKPYRDHINKDLDSVLAYCPENLD
KNSGKWQTTIGNMLADACVQRGDIIFKSRENKTIDFCLLNFGGIRAGLPKGNVTSRSAFEIMPFENNLVVLALKGEQVLE
ITSYFIKTQKAQPLSGMTFTIAKDKTVKNILIQGKPFDINKTYYVATNDYLANGGDNMSFFAKNTQKFDLNYKLRNVLID
YFKQVKTIPVSKDIRITEE
>Mature_259_residues
MVKLKKYNRFLKLFVIFLTLFSIISCSNKNYNLTKIEGKQIPVTEKGTETPEIEKFIKPYRDHINKDLDSVLAYCPENLD
KNSGKWQTTIGNMLADACVQRGDIIFKSRENKTIDFCLLNFGGIRAGLPKGNVTSRSAFEIMPFENNLVVLALKGEQVLE
ITSYFIKTQKAQPLSGMTFTIAKDKTVKNILIQGKPFDINKTYYVATNDYLANGGDNMSFFAKNTQKFDLNYKLRNVLID
YFKQVKTIPVSKDIRITEE

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1786687, Length=124, Percent_Identity=29.8387096774194, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 29605; Mature: 29605

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLKKYNRFLKLFVIFLTLFSIISCSNKNYNLTKIEGKQIPVTEKGTETPEIEKFIKPY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHH
RDHINKDLDSVLAYCPENLDKNSGKWQTTIGNMLADACVQRGDIIFKSRENKTIDFCLLN
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEE
FGGIRAGLPKGNVTSRSAFEIMPFENNLVVLALKGEQVLEITSYFIKTQKAQPLSGMTFT
CCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEE
IAKDKTVKNILIQGKPFDINKTYYVATNDYLANGGDNMSFFAKNTQKFDLNYKLRNVLID
EECCCCEEEEEECCCCCCCCCEEEEEECCEEECCCCCEEEEECCCEEEEECHHHHHHHHH
YFKQVKTIPVSKDIRITEE
HHHHHHCCCCCCCEEEECC
>Mature Secondary Structure
MVKLKKYNRFLKLFVIFLTLFSIISCSNKNYNLTKIEGKQIPVTEKGTETPEIEKFIKPY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHH
RDHINKDLDSVLAYCPENLDKNSGKWQTTIGNMLADACVQRGDIIFKSRENKTIDFCLLN
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEE
FGGIRAGLPKGNVTSRSAFEIMPFENNLVVLALKGEQVLEITSYFIKTQKAQPLSGMTFT
CCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEE
IAKDKTVKNILIQGKPFDINKTYYVATNDYLANGGDNMSFFAKNTQKFDLNYKLRNVLID
EECCCCEEEEEECCCCCCCCCEEEEEECCEEECCCCCEEEEECCCEEEEECHHHHHHHHH
YFKQVKTIPVSKDIRITEE
HHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]