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The map label for this gene is smpB

Identifier: 146301185

GI number: 146301185

Start: 4110999

End: 4111454

Strand: Direct

Name: smpB

Synonym: Fjoh_3443

Alternate gene names: 146301185

Gene position: 4110999-4111454 (Clockwise)

Preceding gene: 146301182

Following gene: 146301186

Centisome position: 67.43

GC content: 32.24

Gene sequence:

>456_bases
ATGTTAAAATCAGTCAATATACTTAATAAAAGAGCCCGGTTTGATTACGAAATAATCGACACCTATACTGCCGGAATTGT
TTTGGCAGGAACCGAAATTAAATCGATACGTTTAGGAAAAGCGAATATTACAGAAAGCTTCTGCGAATTCAGCGGGATTG
AACTTTTTGCAATCAATACTTATATAGAGGAATATTCATTTGGGAATCAATTCAACCATAAATCAAGAAGTGAACGAAAA
TTGCTTTTAAATAAAAAAGAATTAAAAAGCCTTCATAAAAGTGTTCAGGCAAAAGGTCTTACCATTGTACCTTTAAAATT
GTTTACCAATGAAAAAGGTCTTGCAAAATTGCAGATTGGTCTTTGTAAAGGAAAGAAAAACTACGACAAACGTGAATCTC
TAAAAGAGCAGGATACAAAACGCGACTTAGACAGAATTAAAAAAGCATATAACTAA

Upstream 100 bases:

>100_bases
TAAGCATTTTTGAGAAGGATGATTAAAATCATAGATTAAAAAAAATCTTTAATTATATTTGAGCAGTTTATATTCCTGCA
ATTTTCTTACTTTTGTACCC

Downstream 100 bases:

>100_bases
ACACGGATTTAATTACTTAAATCTGTGTTTATCAAAATTTTATAGTTATTTTTACTACAAACATATAACTGTAAAATATG
AAAAAGTACTTAATTCTACT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MLKSVNILNKRARFDYEIIDTYTAGIVLAGTEIKSIRLGKANITESFCEFSGIELFAINTYIEEYSFGNQFNHKSRSERK
LLLNKKELKSLHKSVQAKGLTIVPLKLFTNEKGLAKLQIGLCKGKKNYDKRESLKEQDTKRDLDRIKKAYN

Sequences:

>Translated_151_residues
MLKSVNILNKRARFDYEIIDTYTAGIVLAGTEIKSIRLGKANITESFCEFSGIELFAINTYIEEYSFGNQFNHKSRSERK
LLLNKKELKSLHKSVQAKGLTIVPLKLFTNEKGLAKLQIGLCKGKKNYDKRESLKEQDTKRDLDRIKKAYN
>Mature_151_residues
MLKSVNILNKRARFDYEIIDTYTAGIVLAGTEIKSIRLGKANITESFCEFSGIELFAINTYIEEYSFGNQFNHKSRSERK
LLLNKKELKSLHKSVQAKGLTIVPLKLFTNEKGLAKLQIGLCKGKKNYDKRESLKEQDTKRDLDRIKKAYN

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=143, Percent_Identity=40.5594405594406, Blast_Score=96, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_FLAJ1 (A5FEB8)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001195776.1
- ProteinModelPortal:   A5FEB8
- SMR:   A5FEB8
- STRING:   A5FEB8
- GeneID:   5089644
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_3443
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   FJOH376686:FJOH_3443-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17365; Mature: 17365

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSVNILNKRARFDYEIIDTYTAGIVLAGTEIKSIRLGKANITESFCEFSGIELFAINT
CCCCCHHHHHHHHCCHHHHHHHCCCEEEECCCEEEEECCCCHHHHHHHHCCCEEEEEEHH
YIEEYSFGNQFNHKSRSERKLLLNKKELKSLHKSVQAKGLTIVPLKLFTNEKGLAKLQIG
HHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEE
LCKGKKNYDKRESLKEQDTKRDLDRIKKAYN
EECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKSVNILNKRARFDYEIIDTYTAGIVLAGTEIKSIRLGKANITESFCEFSGIELFAINT
CCCCCHHHHHHHHCCHHHHHHHCCCEEEECCCEEEEECCCCHHHHHHHHCCCEEEEEEHH
YIEEYSFGNQFNHKSRSERKLLLNKKELKSLHKSVQAKGLTIVPLKLFTNEKGLAKLQIG
HHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEE
LCKGKKNYDKRESLKEQDTKRDLDRIKKAYN
EECCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA