Click here to switch to the map view.

The map label for this gene is proB [H]

Identifier: 146301173

GI number: 146301173

Start: 4098466

End: 4099227

Strand: Reverse

Name: proB [H]

Synonym: Fjoh_3431

Alternate gene names: 146301173

Gene position: 4099227-4098466 (Counterclockwise)

Preceding gene: 146301174

Following gene: 146301172

Centisome position: 67.23

GC content: 32.28

Gene sequence:

>762_bases
ATGGGTAAGAAACGGATTTTATTAAAAATAGGAAGTAATACTTTAACCAAAGAAACCAATCATATTTCGCGGGGAAAGAT
TGAAGACCTCGGAATACAGATTGCTGCTTTAAATGACGAATATGAGTTTATCATAGTTAGTTCAGGAGCAATTGCAGCAG
CAAAGCAGTTTGTGAAACTTGATAATCAGGATAAAGATGTTTTTGTAAAACAGGCTTTGGCTTCAATTGGACAGCCTCAT
TTAATGCGGATTTACAATGAAAATTTTAAGGATTTAGGATTAAATACTTCTCAGTGTTTGCTTTCTTATTCTGATTTTGA
AAAAGAACAGACTAAAAAGAACATTGTAAATACAATTAATGTATTAGTTAAAAACAATTATATTCCGATTATCAATGAAA
ATGATACCGTTGCCACAGATGAGATTCGGTTTGGAGATAATGATAAATTAGCGGCTTTAACAGCAGTTCTTTTAAATGTT
GATATTCTGATAATTGCCACTAATACAAACGGAATTTACACAAAGAATTCAATTCATGATGAAAATCCGGAGACGATAAA
ATTGGTAAATGATTTAAAATCACTCGAAAAAGAAATCGGCGAATCAAAATCATCACACGGAACAGGAGGAATGCAATCTA
AAATAGAAGCAGCAGGAATTTCAAAAGCTGCCAACATTGAAACCTGGATTGTCAATGGACTGAATGATAATTTTATTTTA
AAGGCACTCAAGGACGAAATTCCTTTTACCAAGATTGTGTAA

Upstream 100 bases:

>100_bases
TTAAAGATATAGCTTTGCGAACTTAGCGTTTGTAAACATAAAGTATATAGAAAAACCTTGCGCACTTTGCGGTTAAAAAA
ATCGCAGCAAAACAACAACA

Downstream 100 bases:

>100_bases
AAAGGTTTAATTGTTTAATCGTTAATTCGGTTAATCGATTAAAAAAGAACATTAAAAAACAAAAATGGATAAAAGTTAGA
TTTGTTTAAGCAGTAAAACG

Product: glutamate 5-kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MGKKRILLKIGSNTLTKETNHISRGKIEDLGIQIAALNDEYEFIIVSSGAIAAAKQFVKLDNQDKDVFVKQALASIGQPH
LMRIYNENFKDLGLNTSQCLLSYSDFEKEQTKKNIVNTINVLVKNNYIPIINENDTVATDEIRFGDNDKLAALTAVLLNV
DILIIATNTNGIYTKNSIHDENPETIKLVNDLKSLEKEIGESKSSHGTGGMQSKIEAAGISKAANIETWIVNGLNDNFIL
KALKDEIPFTKIV

Sequences:

>Translated_253_residues
MGKKRILLKIGSNTLTKETNHISRGKIEDLGIQIAALNDEYEFIIVSSGAIAAAKQFVKLDNQDKDVFVKQALASIGQPH
LMRIYNENFKDLGLNTSQCLLSYSDFEKEQTKKNIVNTINVLVKNNYIPIINENDTVATDEIRFGDNDKLAALTAVLLNV
DILIIATNTNGIYTKNSIHDENPETIKLVNDLKSLEKEIGESKSSHGTGGMQSKIEAAGISKAANIETWIVNGLNDNFIL
KALKDEIPFTKIV
>Mature_252_residues
GKKRILLKIGSNTLTKETNHISRGKIEDLGIQIAALNDEYEFIIVSSGAIAAAKQFVKLDNQDKDVFVKQALASIGQPHL
MRIYNENFKDLGLNTSQCLLSYSDFEKEQTKKNIVNTINVLVKNNYIPIINENDTVATDEIRFGDNDKLAALTAVLLNVD
ILIIATNTNGIYTKNSIHDENPETIKLVNDLKSLEKEIGESKSSHGTGGMQSKIEAAGISKAANIETWIVNGLNDNFILK
ALKDEIPFTKIV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=264, Percent_Identity=28.4090909090909, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI62912457, Length=262, Percent_Identity=28.6259541984733, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1786437, Length=236, Percent_Identity=36.0169491525424, Blast_Score=149, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17569969, Length=259, Percent_Identity=31.2741312741313, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17569967, Length=259, Percent_Identity=31.2741312741313, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321822, Length=241, Percent_Identity=34.0248962655602, Blast_Score=125, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=231, Percent_Identity=35.0649350649351, Blast_Score=121, Evalue=8e-29,
Organism=Drosophila melanogaster, GI21357643, Length=269, Percent_Identity=31.5985130111524, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 27946; Mature: 27815

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKRILLKIGSNTLTKETNHISRGKIEDLGIQIAALNDEYEFIIVSSGAIAAAKQFVKL
CCCCEEEEEECCCCCCCCCCCCCCCCCHHHCEEEEEECCCEEEEEEECCHHHHHHHHHHC
DNQDKDVFVKQALASIGQPHLMRIYNENFKDLGLNTSQCLLSYSDFEKEQTKKNIVNTIN
CCCCHHHHHHHHHHHCCCCCEEHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLVKNNYIPIINENDTVATDEIRFGDNDKLAALTAVLLNVDILIIATNTNGIYTKNSIHD
HEEECCEEEEECCCCCEEECEEEECCCCHHHHHHHHHHCCEEEEEEECCCCEEEECCCCC
ENPETIKLVNDLKSLEKEIGESKSSHGTGGMQSKIEAAGISKAANIETWIVNGLNDNFIL
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCHHHH
KALKDEIPFTKIV
HHHHHCCCEECCC
>Mature Secondary Structure 
GKKRILLKIGSNTLTKETNHISRGKIEDLGIQIAALNDEYEFIIVSSGAIAAAKQFVKL
CCCEEEEEECCCCCCCCCCCCCCCCCHHHCEEEEEECCCEEEEEEECCHHHHHHHHHHC
DNQDKDVFVKQALASIGQPHLMRIYNENFKDLGLNTSQCLLSYSDFEKEQTKKNIVNTIN
CCCCHHHHHHHHHHHCCCCCEEHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLVKNNYIPIINENDTVATDEIRFGDNDKLAALTAVLLNVDILIIATNTNGIYTKNSIHD
HEEECCEEEEECCCCCEEECEEEECCCCHHHHHHHHHHCCEEEEEEECCCCEEEECCCCC
ENPETIKLVNDLKSLEKEIGESKSSHGTGGMQSKIEAAGISKAANIETWIVNGLNDNFIL
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCHHHH
KALKDEIPFTKIV
HHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]