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The map label for this gene is argF' [H]

Identifier: 146301172

GI number: 146301172

Start: 4097378

End: 4098325

Strand: Reverse

Name: argF' [H]

Synonym: Fjoh_3430

Alternate gene names: 146301172

Gene position: 4098325-4097378 (Counterclockwise)

Preceding gene: 146301173

Following gene: 146301171

Centisome position: 67.22

GC content: 36.29

Gene sequence:

>948_bases
ATGAACTACATCTCAATAAAAGATATCGACTCATTATCAAAATGGGTTAAAAGCGCGATCAAAATCAAAAAAGATCCGCT
TAAAAATAAAAAACTAGGTAAAAACAAAACCTTAGGAATGTTATTCTTCAATCCGAGTTTAAGAACGCGTTTGAGTACAC
AAAAAGCGGCTTTAAACTTAGGAATGAATGTTATGGTAATGAACTTTACCAATGAAGGCTGGACTTTAGAATTTGAAGAC
GGAGCGGTTATGAATTCTGGTGCTTCAGAACATATTAAAGAAGCTGCAGAAGTAGTTTCTCAATACTGTGATATTATCGC
AATTCGTGCTTTTGCTGGTTTGGTTGATAAAGAAAAAGATTATCAGGAAACTGTAATTTCAGGATTTTTGAAATACGCTA
CAGTGCCAATCGTAAACATGGAAAGTGCGGTTCGTCACCCATTGCAGTCTTTGGCAGATGCCATTACTATGGAAGAATAC
AAAACCAAACACAAACCAAAAGTGGTTCTTTCTTGGGCGCCGCACCCAAAAGCTTTGCCTCAGGCAGTTGCGAATTCATT
TGTAGAAATGATGCAGATGCAAAAAGACATGGATTTTGTAATTACACATCCAGAAGGTTACGAATTAAGCCCAGAAATCA
CACAAGACTGCAAAATCGAATACGATCAAGATAAAGCTTTTGAAAATGCCGATTTCGTTTACGTAAAAAACTGGAGTAAT
TTCAACGATTACGGAAAAGTAACCAACAGTGATCCAAACTGGACAGTTACGGCAGAAAAAATGGCTTTAACCAACAACGG
AAAATTCATGCACTGTCTTCCAGTTCGTCGTAACGTAATTGTAAGCGACGAAGTTTTAGATGGAGAAAACTCAATCGTAA
TCGAACAAGCGAATAACAGAACGTATTCAGCACAATTAGTTTTACAAAAGATTCTTAAGAAAATTTAA

Upstream 100 bases:

>100_bases
AAAAAAGAACATTAAAAAACAAAAATGGATAAAAGTTAGATTTGTTTAAGCAGTAAAACGATTAAACAAATCAACGATTA
ACCGATTAAACAAAAAAACA

Downstream 100 bases:

>100_bases
TTTAAGGTGCAAAGGTTCAAAGTTGCAAAGTTGCAGAGGTTTAAGATCTTGAGAAAAACCTTTGTACCTTTGTACCTCTG
TACCTCTGTACCTCTGAACC

Product: aspartate/ornithine carbamoyltransferase carbamoyl phosphate-binding subunit

Products: NA

Alternate protein names: AOTCase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MNYISIKDIDSLSKWVKSAIKIKKDPLKNKKLGKNKTLGMLFFNPSLRTRLSTQKAALNLGMNVMVMNFTNEGWTLEFED
GAVMNSGASEHIKEAAEVVSQYCDIIAIRAFAGLVDKEKDYQETVISGFLKYATVPIVNMESAVRHPLQSLADAITMEEY
KTKHKPKVVLSWAPHPKALPQAVANSFVEMMQMQKDMDFVITHPEGYELSPEITQDCKIEYDQDKAFENADFVYVKNWSN
FNDYGKVTNSDPNWTVTAEKMALTNNGKFMHCLPVRRNVIVSDEVLDGENSIVIEQANNRTYSAQLVLQKILKKI

Sequences:

>Translated_315_residues
MNYISIKDIDSLSKWVKSAIKIKKDPLKNKKLGKNKTLGMLFFNPSLRTRLSTQKAALNLGMNVMVMNFTNEGWTLEFED
GAVMNSGASEHIKEAAEVVSQYCDIIAIRAFAGLVDKEKDYQETVISGFLKYATVPIVNMESAVRHPLQSLADAITMEEY
KTKHKPKVVLSWAPHPKALPQAVANSFVEMMQMQKDMDFVITHPEGYELSPEITQDCKIEYDQDKAFENADFVYVKNWSN
FNDYGKVTNSDPNWTVTAEKMALTNNGKFMHCLPVRRNVIVSDEVLDGENSIVIEQANNRTYSAQLVLQKILKKI
>Mature_315_residues
MNYISIKDIDSLSKWVKSAIKIKKDPLKNKKLGKNKTLGMLFFNPSLRTRLSTQKAALNLGMNVMVMNFTNEGWTLEFED
GAVMNSGASEHIKEAAEVVSQYCDIIAIRAFAGLVDKEKDYQETVISGFLKYATVPIVNMESAVRHPLQSLADAITMEEY
KTKHKPKVVLSWAPHPKALPQAVANSFVEMMQMQKDMDFVITHPEGYELSPEITQDCKIEYDQDKAFENADFVYVKNWSN
FNDYGKVTNSDPNWTVTAEKMALTNNGKFMHCLPVRRNVIVSDEVLDGENSIVIEQANNRTYSAQLVLQKILKKI

Specific function: Catalyzes the conversion of N-acetylornithine to N- acetylcitrulline in an alternative arginine biosynthesis pathway. The enzyme has no activity with ornithine [H]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=315, Percent_Identity=24.1269841269841, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1790703, Length=341, Percent_Identity=24.633431085044, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI48994908, Length=288, Percent_Identity=25.6944444444444, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.9 [H]

Molecular weight: Translated: 35686; Mature: 35686

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYISIKDIDSLSKWVKSAIKIKKDPLKNKKLGKNKTLGMLFFNPSLRTRLSTQKAALNL
CCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHC
GMNVMVMNFTNEGWTLEFEDGAVMNSGASEHIKEAAEVVSQYCDIIAIRAFAGLVDKEKD
CCEEEEEEECCCCCEEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YQETVISGFLKYATVPIVNMESAVRHPLQSLADAITMEEYKTKHKPKVVLSWAPHPKALP
HHHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHH
QAVANSFVEMMQMQKDMDFVITHPEGYELSPEITQDCKIEYDQDKAFENADFVYVKNWSN
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEECCCC
FNDYGKVTNSDPNWTVTAEKMALTNNGKFMHCLPVRRNVIVSDEVLDGENSIVIEQANNR
CCCCCCCCCCCCCEEEEEEEEEEECCCCEEEEEECCCCEEEECCEECCCCCEEEEECCCC
TYSAQLVLQKILKKI
CHHHHHHHHHHHHHC
>Mature Secondary Structure
MNYISIKDIDSLSKWVKSAIKIKKDPLKNKKLGKNKTLGMLFFNPSLRTRLSTQKAALNL
CCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHC
GMNVMVMNFTNEGWTLEFEDGAVMNSGASEHIKEAAEVVSQYCDIIAIRAFAGLVDKEKD
CCEEEEEEECCCCCEEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YQETVISGFLKYATVPIVNMESAVRHPLQSLADAITMEEYKTKHKPKVVLSWAPHPKALP
HHHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHH
QAVANSFVEMMQMQKDMDFVITHPEGYELSPEITQDCKIEYDQDKAFENADFVYVKNWSN
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEECCCC
FNDYGKVTNSDPNWTVTAEKMALTNNGKFMHCLPVRRNVIVSDEVLDGENSIVIEQANNR
CCCCCCCCCCCCCEEEEEEEEEEECCCCEEEEEECCCCEEEECCEECCCCCEEEEECCCC
TYSAQLVLQKILKKI
CHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]