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The map label for this gene is tyrS2 [H]

Identifier: 146301128

GI number: 146301128

Start: 4044092

End: 4045294

Strand: Direct

Name: tyrS2 [H]

Synonym: Fjoh_3386

Alternate gene names: 146301128

Gene position: 4044092-4045294 (Clockwise)

Preceding gene: 146301127

Following gene: 146301133

Centisome position: 66.33

GC content: 32.5

Gene sequence:

>1203_bases
ATGGAAACAATTCAAAAACTTAAAGAAAACACCGCAATCTTCTTACCGGAAAATGGTCTGGAAGAAAAATTAAATCAGGC
TGAAAAAGAAAACAGAAAACTGATTATTAAACTGGGTTTTGATCCTACTGCTCCTGATTTGCATTTAGGACATGCCGTTG
TATTAAAAAAATTAAAGCAATTTCAGGATTTAGGACACCAAATTGTGATTTTGGTGGGAAGTTTTACAGCCAGAATTGGC
GATCCAACCGGAAAAAACAAAAGCAGAAAACCTCTAAATGTCCAAGAAGTACAGCACAATGCCGAAACTTATATTGCTCA
ATTATCAAAAATCATTGATGTAGAGAAAGCGCAGATTGTTTTTAATTCTGACTGGCTGGATTCACTTTCTTTTTCTGAAG
TAATTCAGATCATGTCAAACGTAACGGTTGCGCAGCTCATGCAGCGAAATGATTTTAATAAACGTTTTACTGAAAATACA
CCTATTGCCATGCATGAATTAGTGTACCCAATTTTGCAGGGTTTTGATTCGGTTAAAATTAATGCCGATATTGAAATGGG
CGGTACAGACCAGCTTTTTAATTGCACTATGGGAAGACAATTGCAGGAAACTTTTAAAAAATCTCCGCAAATTGTAATGT
GCATGCCTTTATTAAGAGGATTGGACGGAAAGGAAAAAATGAGCAAATCGCTCAATAATATCATTGGTTTAACAGATGAA
CCTAATGAAATGTTTGGCAAAACAATGTCAATTCCTGATGATCTTATTTTGGAATTCTTAGAACTCACAACTGATTTTTC
TACAAAAGAAAAACAGGAAATCAAAAATCGGTTGAAAGAAGAAAACCCAATGAACATCAAAAAAATAATTGCAAAAAATA
TCATCAATCAATACCATGATGAGAAAAAAGCAGATAAAGCAGAAGATTTTTTCGTAAATCAGTTTCAGAACAAAAACTTT
GAGGAAAAATCGTTTGTTCCTGTTTTGATTTCTTCTTTAGAAAATAAATCTGATCAAATGGCTTTAATTGATCTTTGTGC
TTTTATAAAAAATGACATTACAAAATCAGCAAACAGAAGACTGATCGAAAGCGGTGCTGTTCAAGTAAATAATTTAAAAA
TTACAAATCCGTACGAATTGATTGAGTTGAAAAAGGAAACCAAAATTAAAATTGGAAAACGAAATTTCTATGAATTGGTT
TAG

Upstream 100 bases:

>100_bases
TGCATATAATTCAACGTATTCACGACTGTAAATTAGTATTATAAATTATATAAACCTTAAAGCCCGGGCATTTTGTCCGG
GCTTTTTTATTTTCAAAAAA

Downstream 100 bases:

>100_bases
ATTTTAGGTTTAGAGAAACAGAGTGACATAGAGACAAAGGTTTCTTTGATTGTCTTTTTTCGAAAAACAAAAGGGATAAA
CAATTAAGTCTATCCCTTTT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase 2; TyrRS 2 [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
METIQKLKENTAIFLPENGLEEKLNQAEKENRKLIIKLGFDPTAPDLHLGHAVVLKKLKQFQDLGHQIVILVGSFTARIG
DPTGKNKSRKPLNVQEVQHNAETYIAQLSKIIDVEKAQIVFNSDWLDSLSFSEVIQIMSNVTVAQLMQRNDFNKRFTENT
PIAMHELVYPILQGFDSVKINADIEMGGTDQLFNCTMGRQLQETFKKSPQIVMCMPLLRGLDGKEKMSKSLNNIIGLTDE
PNEMFGKTMSIPDDLILEFLELTTDFSTKEKQEIKNRLKEENPMNIKKIIAKNIINQYHDEKKADKAEDFFVNQFQNKNF
EEKSFVPVLISSLENKSDQMALIDLCAFIKNDITKSANRRLIESGAVQVNNLKITNPYELIELKKETKIKIGKRNFYELV

Sequences:

>Translated_400_residues
METIQKLKENTAIFLPENGLEEKLNQAEKENRKLIIKLGFDPTAPDLHLGHAVVLKKLKQFQDLGHQIVILVGSFTARIG
DPTGKNKSRKPLNVQEVQHNAETYIAQLSKIIDVEKAQIVFNSDWLDSLSFSEVIQIMSNVTVAQLMQRNDFNKRFTENT
PIAMHELVYPILQGFDSVKINADIEMGGTDQLFNCTMGRQLQETFKKSPQIVMCMPLLRGLDGKEKMSKSLNNIIGLTDE
PNEMFGKTMSIPDDLILEFLELTTDFSTKEKQEIKNRLKEENPMNIKKIIAKNIINQYHDEKKADKAEDFFVNQFQNKNF
EEKSFVPVLISSLENKSDQMALIDLCAFIKNDITKSANRRLIESGAVQVNNLKITNPYELIELKKETKIKIGKRNFYELV
>Mature_400_residues
METIQKLKENTAIFLPENGLEEKLNQAEKENRKLIIKLGFDPTAPDLHLGHAVVLKKLKQFQDLGHQIVILVGSFTARIG
DPTGKNKSRKPLNVQEVQHNAETYIAQLSKIIDVEKAQIVFNSDWLDSLSFSEVIQIMSNVTVAQLMQRNDFNKRFTENT
PIAMHELVYPILQGFDSVKINADIEMGGTDQLFNCTMGRQLQETFKKSPQIVMCMPLLRGLDGKEKMSKSLNNIIGLTDE
PNEMFGKTMSIPDDLILEFLELTTDFSTKEKQEIKNRLKEENPMNIKKIIAKNIINQYHDEKKADKAEDFFVNQFQNKNF
EEKSFVPVLISSLENKSDQMALIDLCAFIKNDITKSANRRLIESGAVQVNNLKITNPYELIELKKETKIKIGKRNFYELV

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=433, Percent_Identity=24.9422632794457, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1787925, Length=412, Percent_Identity=27.4271844660194, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI25145802, Length=285, Percent_Identity=28.7719298245614, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325160, Length=327, Percent_Identity=25.3822629969419, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20130383, Length=403, Percent_Identity=24.317617866005, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45768; Mature: 45768

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIQKLKENTAIFLPENGLEEKLNQAEKENRKLIIKLGFDPTAPDLHLGHAVVLKKLKQ
CCHHHHHHCCCEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
FQDLGHQIVILVGSFTARIGDPTGKNKSRKPLNVQEVQHNAETYIAQLSKIIDVEKAQIV
HHHCCCEEEEEEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEE
FNSDWLDSLSFSEVIQIMSNVTVAQLMQRNDFNKRFTENTPIAMHELVYPILQGFDSVKI
ECCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHCCCCCEEE
NADIEMGGTDQLFNCTMGRQLQETFKKSPQIVMCMPLLRGLDGKEKMSKSLNNIIGLTDE
EEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCHHHHHHHHHHHCCCCCC
PNEMFGKTMSIPDDLILEFLELTTDFSTKEKQEIKNRLKEENPMNIKKIIAKNIINQYHD
CHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EKKADKAEDFFVNQFQNKNFEEKSFVPVLISSLENKSDQMALIDLCAFIKNDITKSANRR
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LIESGAVQVNNLKITNPYELIELKKETKIKIGKRNFYELV
HHHCCCEEEEEEEECCCHHHHHHHHHHHEEECCCHHHHCC
>Mature Secondary Structure
METIQKLKENTAIFLPENGLEEKLNQAEKENRKLIIKLGFDPTAPDLHLGHAVVLKKLKQ
CCHHHHHHCCCEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
FQDLGHQIVILVGSFTARIGDPTGKNKSRKPLNVQEVQHNAETYIAQLSKIIDVEKAQIV
HHHCCCEEEEEEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEE
FNSDWLDSLSFSEVIQIMSNVTVAQLMQRNDFNKRFTENTPIAMHELVYPILQGFDSVKI
ECCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHCCCCCEEE
NADIEMGGTDQLFNCTMGRQLQETFKKSPQIVMCMPLLRGLDGKEKMSKSLNNIIGLTDE
EEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCHHHHHHHHHHHCCCCCC
PNEMFGKTMSIPDDLILEFLELTTDFSTKEKQEIKNRLKEENPMNIKKIIAKNIINQYHD
CHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EKKADKAEDFFVNQFQNKNFEEKSFVPVLISSLENKSDQMALIDLCAFIKNDITKSANRR
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LIESGAVQVNNLKITNPYELIELKKETKIKIGKRNFYELV
HHHCCCEEEEEEEECCCHHHHHHHHHHHEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]