Click here to switch to the map view.

The map label for this gene is fadH [C]

Identifier: 146301127

GI number: 146301127

Start: 4042607

End: 4043713

Strand: Direct

Name: fadH [C]

Synonym: Fjoh_3385

Alternate gene names: 146301127

Gene position: 4042607-4043713 (Clockwise)

Preceding gene: 146301126

Following gene: 146301128

Centisome position: 66.31

GC content: 39.57

Gene sequence:

>1107_bases
ATGAGCACAAACAACCTGTTTTCTCCGTTTAACCTAAAAACGTTAAACTTAAAAAACCGAATCGTAATGGCGCCTATGAC
GCGCTCATTTTCTCCAAACGGAGTTCCAACTGATGATGTAGCAGCTTACTACCAAAAAAGAGCTGAAGGTGAAGTTGGTT
TAATATTATCTGAAGGAACTGTTATCGACAGAAAATCATCATCTAATGATGCAAATGTTCCTCATTTTTATGGCGATCTA
GCTTTAAACGGCTGGAAAAAAGTTATTGACGAAGTTCATGCCGCCGGCGGAAAAATGGGCCCGCAGATCTGGCATATGGG
AATTATGGACAACCATCATTCCGGCTGGGTTCCTCCTGTTCCATTTGAAGGTCCGTCTGGATTAAACCGTCCTGATTTTA
GAAACGGAATTGCAATGTCTGAAAAAGATATTGAAGACACGATTCTTGCTTTTGGTAAAGCTGCTGCCGATGCTAAAAGA
CTTGGTTTTGATACTATCGAAATTCACGGTGCGCACGGTTATTTAATTGATCAGTTTTTTAGAGCCGAAACGAATTTACG
TGAAGACATTTATGGCGGAAAAACACTGCCGGAACGTAATCGTTTTGCTATTGAGGTTATAAAAGAAATCAGAAAACAAG
TAGGAAATGATTTTGCTGTAATCATGCGTTTTTCTCAATTTAAACCTTCTGATTACAATTATAAACTGGCTAAAAATCCG
CAGGAATTAGAAGCTTGGCTTACTCCTCTTGTTGATGCCGGAGTTGATATTATTCACGCTTCGCAGCGTCGTTTCTGGGA
ACCTGAATTTGAAGGATCTGATTTAAACTTTGCGGGATGGGCTAAAAAAGTAACCGGAGCGCCAACTATTACAGTAGGTT
CTGTTGGTCTATCTGGTGATTTCTTTGGTGCTTTTGCCGGAGAAAGTTCTGAGCCGACTTCTTTAGATGAATTAAACAGA
CGTTTCGACAGAGGCGATTTTGATTTAGTTGCCGTAGGAAGACCTCTTTTATCTGATCCTAACTGGGTTGCTAAAATTAA
AGCAGGAAAAACAGATCAGTTAAAAGGTTTCAGTAAAGAAGCTTTAGGAGCTTTAGTTTTAGAATAA

Upstream 100 bases:

>100_bases
TATCGAAGCCAAACGTGTTGGACAATGGACTTATTATAAACGTAACGAAGGTGCCTTTGAAGCACTCAGTAAATTAATTC
AATCTAATTTGTAAATTACT

Downstream 100 bases:

>100_bases
GTTTTTTAAAGGTTAAGAGTTACAAAGGTTCAAAGGAACAAAGGCTTTCTTTTTGAACTAAATATACAAAAACTAACAAA
ACTGAAATTCAATATTTTTA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSTNNLFSPFNLKTLNLKNRIVMAPMTRSFSPNGVPTDDVAAYYQKRAEGEVGLILSEGTVIDRKSSSNDANVPHFYGDL
ALNGWKKVIDEVHAAGGKMGPQIWHMGIMDNHHSGWVPPVPFEGPSGLNRPDFRNGIAMSEKDIEDTILAFGKAAADAKR
LGFDTIEIHGAHGYLIDQFFRAETNLREDIYGGKTLPERNRFAIEVIKEIRKQVGNDFAVIMRFSQFKPSDYNYKLAKNP
QELEAWLTPLVDAGVDIIHASQRRFWEPEFEGSDLNFAGWAKKVTGAPTITVGSVGLSGDFFGAFAGESSEPTSLDELNR
RFDRGDFDLVAVGRPLLSDPNWVAKIKAGKTDQLKGFSKEALGALVLE

Sequences:

>Translated_368_residues
MSTNNLFSPFNLKTLNLKNRIVMAPMTRSFSPNGVPTDDVAAYYQKRAEGEVGLILSEGTVIDRKSSSNDANVPHFYGDL
ALNGWKKVIDEVHAAGGKMGPQIWHMGIMDNHHSGWVPPVPFEGPSGLNRPDFRNGIAMSEKDIEDTILAFGKAAADAKR
LGFDTIEIHGAHGYLIDQFFRAETNLREDIYGGKTLPERNRFAIEVIKEIRKQVGNDFAVIMRFSQFKPSDYNYKLAKNP
QELEAWLTPLVDAGVDIIHASQRRFWEPEFEGSDLNFAGWAKKVTGAPTITVGSVGLSGDFFGAFAGESSEPTSLDELNR
RFDRGDFDLVAVGRPLLSDPNWVAKIKAGKTDQLKGFSKEALGALVLE
>Mature_367_residues
STNNLFSPFNLKTLNLKNRIVMAPMTRSFSPNGVPTDDVAAYYQKRAEGEVGLILSEGTVIDRKSSSNDANVPHFYGDLA
LNGWKKVIDEVHAAGGKMGPQIWHMGIMDNHHSGWVPPVPFEGPSGLNRPDFRNGIAMSEKDIEDTILAFGKAAADAKRL
GFDTIEIHGAHGYLIDQFFRAETNLREDIYGGKTLPERNRFAIEVIKEIRKQVGNDFAVIMRFSQFKPSDYNYKLAKNPQ
ELEAWLTPLVDAGVDIIHASQRRFWEPEFEGSDLNFAGWAKKVTGAPTITVGSVGLSGDFFGAFAGESSEPTSLDELNRR
FDRGDFDLVAVGRPLLSDPNWVAKIKAGKTDQLKGFSKEALGALVLE

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=370, Percent_Identity=28.6486486486486, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787939, Length=370, Percent_Identity=30, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17540738, Length=369, Percent_Identity=25.4742547425474, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17564188, Length=222, Percent_Identity=29.7297297297297, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559804, Length=361, Percent_Identity=25.7617728531856, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17565138, Length=221, Percent_Identity=26.2443438914027, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17559802, Length=222, Percent_Identity=28.3783783783784, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6321973, Length=244, Percent_Identity=26.6393442622951, Blast_Score=86, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 40665; Mature: 40534

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNNLFSPFNLKTLNLKNRIVMAPMTRSFSPNGVPTDDVAAYYQKRAEGEVGLILSEGT
CCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCE
VIDRKSSSNDANVPHFYGDLALNGWKKVIDEVHAAGGKMGPQIWHMGIMDNHHSGWVPPV
EEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEECCCCCCCCCC
PFEGPSGLNRPDFRNGIAMSEKDIEDTILAFGKAAADAKRLGFDTIEIHGAHGYLIDQFF
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
RAETNLREDIYGGKTLPERNRFAIEVIKEIRKQVGNDFAVIMRFSQFKPSDYNYKLAKNP
HHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCEEECCCH
QELEAWLTPLVDAGVDIIHASQRRFWEPEFEGSDLNFAGWAKKVTGAPTITVGSVGLSGD
HHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCC
FFGAFAGESSEPTSLDELNRRFDRGDFDLVAVGRPLLSDPNWVAKIKAGKTDQLKGFSKE
EEEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCHHHCCCCHH
ALGALVLE
HHHHHHCC
>Mature Secondary Structure 
STNNLFSPFNLKTLNLKNRIVMAPMTRSFSPNGVPTDDVAAYYQKRAEGEVGLILSEGT
CCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCE
VIDRKSSSNDANVPHFYGDLALNGWKKVIDEVHAAGGKMGPQIWHMGIMDNHHSGWVPPV
EEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEECCCCCCCCCC
PFEGPSGLNRPDFRNGIAMSEKDIEDTILAFGKAAADAKRLGFDTIEIHGAHGYLIDQFF
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
RAETNLREDIYGGKTLPERNRFAIEVIKEIRKQVGNDFAVIMRFSQFKPSDYNYKLAKNP
HHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCEEECCCH
QELEAWLTPLVDAGVDIIHASQRRFWEPEFEGSDLNFAGWAKKVTGAPTITVGSVGLSGD
HHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCC
FFGAFAGESSEPTSLDELNRRFDRGDFDLVAVGRPLLSDPNWVAKIKAGKTDQLKGFSKE
EEEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCHHHCCCCHH
ALGALVLE
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]